Restriction map for
pFN21AE0993
(Circular) (Six-base) MAPSORT of: pFN21AE0993.seq Check: 7339 from: 1 to: 6640
pFN21AE0993 6640 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2991 2991
Size: 6640
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4450 278
Size: 53 83 186 936 2914 2468
Fragments arranged by size:
2914 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3983 1937
Size: 2046 4594
Acc65I G'GTAC_C
Cuts at: 3961 3961
Size: 6640
AclI AA'CG_TT
Cuts at: 1563 4767 5140 1563
Size: 3204 373 3063
Fragments arranged by size:
3204 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3450 3672 4703 6087 6355 1632
Size: 1818 222 1031 1384 268 1917
Fragments arranged by size:
1917 1818 1384 1031 268 222
AflII C'TTAA_G
Cuts at: 820 1017 2183 820
Size: 197 1166 5277
Fragments arranged by size:
5277 1166 197
AflIII A'CryG_T
Cuts at: 1203 3657 5539 6516 1203
Size: 2454 1882 977 1327
Fragments arranged by size:
2454 1882 1327 977
AgeI A'CCGG_T
Cuts at: 5471 5471
Size: 6640
AhdI GACnn_n'nnGTC
Cuts at: 2482 2638 2650 5369 2482
Size: 156 12 2719 3753
Fragments arranged by size:
3753 2719 156 12
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6640
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3143 3813 1184
Size: 1959 670 4011
Fragments arranged by size:
4011 1959 670
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3909 3941 3909
Size: 32 6608
AlwNI CAG_nnn'CTG
Cuts at: 2431 2992 3160 3739 3855 3877 3898 4383 5955
Size: 561 168 579 116 22 21 485 1572
Cuts at: 5955 2431
Size: 3116
Fragments arranged by size:
3116 1572 579 561 485 168 116 22
21
ApaLI G'TGCA_C
Cuts at: 4697 5853 4697
Size: 1156 5484
ApoI r'AATT_y
Cuts at: 1493 2012 2361 2844 2923 3949 4175 4327 6436
Size: 519 349 483 79 1026 226 152 2109
Cuts at: 6436 1493
Size: 1697
Fragments arranged by size:
2109 1697 1026 519 483 349 226 152
79
AseI AT'TA_AT
Cuts at: 160 1032 2958 5194 160
Size: 872 1926 2236 1606
Fragments arranged by size:
2236 1926 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6640
AvaI C'yCGr_G
Cuts at: 1870 1943 3965 1870
Size: 73 2022 4545
Fragments arranged by size:
4545 2022 73
BamHI G'GATC_C
Cuts at: 2555 2976 3533 3970 4337 2555
Size: 421 557 437 367 4858
Fragments arranged by size:
4858 557 437 421 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3838 3961 5416 618
Size: 325 217 627 2051 123 1455 1842
Fragments arranged by size:
2051 1842 1455 627 325 217 123
BanII G_rGCy'C
Cuts at: 1391 3959 1391
Size: 2568 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2922 3238 3420 3660 928
Size: 971 1023 316 182 240 3908
Fragments arranged by size:
3908 1023 971 316 240 182
BbvCI CC'TCA_GC
Cuts at: 3044 3044
Size: 6640
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4831 4865 1903
Size: 34 50 34 2810 34 3678
Fragments arranged by size:
3678 2810 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2457 3389 4532 5742 2457
Size: 932 1143 1210 3355
Fragments arranged by size:
3355 1210 1143 932
BclI T'GATC_A
Cuts at: 1548 3900 1548
Size: 2352 4288
BfrBI ATG'CAT
Cuts at: 2452 2664 2696 3449 2452
Size: 212 32 753 5643
Fragments arranged by size:
5643 753 212 32
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5251 136
Size: 107 122 71 631 605 3579 1525
Fragments arranged by size:
3579 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2347 3140 6635 2347
Size: 793 3495 2352
Fragments arranged by size:
3495 2352 793
BlpI GC'TnA_GC
Cuts at: 4057 4057
Size: 6640
Bme1580I G_kGCm'C
Cuts at: 1790 3001 4701 5857 1790
Size: 1211 1700 1156 2573
Fragments arranged by size:
2573 1700 1211 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6640
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4371 5329 442
Size: 416 53 83 399 24 2954 958 1753
Fragments arranged by size:
2954 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3804 1056
Size: 2748 3892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3038 3911 5300 1239
Size: 1799 873 1389 2579
Fragments arranged by size:
2579 1799 1389 873
Bpu10I CC'TnA_GC
Cuts at: 3044 3044
Size: 6640
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3761 3828 4765 5630 5928 3761
Size: 67 937 865 298 4473
Fragments arranged by size:
4473 937 865 298 67
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5303 882
Size: 617 3804 2219
Fragments arranged by size:
3804 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2092 4431 493
Size: 711 888 2339 2702
Fragments arranged by size:
2702 2339 888 711
BsaBI GATnn'nnATC
Cuts at: 6634 6634
Size: 6640
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3023 4447 4829 5464
Size: 53 83 186 936 1490 1424 382 635
Cuts at: 5464 275
Size: 1451
Fragments arranged by size:
1490 1451 1424 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5073 5471 5745 5892 6303 5073
Size: 398 274 147 411 5410
Fragments arranged by size:
5410 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2572 2602 2742 2772 3909
Size: 30 165 30 1169 30 140 30 1137
Cuts at: 3909 3939 1178
Size: 30 3879
Fragments arranged by size:
3879 1169 1137 165 140 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2178 2240 2473 3230 3494 1186
Size: 992 62 233 757 264 4332
Fragments arranged by size:
4332 992 757 264 233 62
BseYI C'CCAG_C
Cuts at: 2269 2434 3537 3739 5843 6339 2269
Size: 165 1103 202 2104 496 2570
Fragments arranged by size:
2570 2104 1103 496 202 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6067
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4090 4851 5879 1540
Size: 275 186 2089 761 1028 2301
Fragments arranged by size:
2301 2089 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4701 4786 5857 4701
Size: 85 1071 5484
Fragments arranged by size:
5484 1071 85
BsmI GAATG_Cn'
Cuts at: 2696 3132 3802 4151 4244 2696
Size: 436 670 349 93 5092
Fragments arranged by size:
5092 670 436 349 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3001 3959 4701 4786 5857 1391
Size: 399 1211 958 742 85 1071 2174
Fragments arranged by size:
2174 1211 1071 958 742 399 85
BspEI T'CCGG_A
Cuts at: 6303 6303
Size: 6640
BspHI T'CATG_A
Cuts at: 4529 6259 4529
Size: 1730 4910
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2991 3324 3995 844
Size: 2147 333 671 3489
Fragments arranged by size:
3489 2147 671 333
BsrBI CCG'CTC
Cuts at: 1394 1823 4527 1394
Size: 429 2704 3507
Fragments arranged by size:
3507 2704 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5135 5309 65
Size: 1183 3887 174 1396
Fragments arranged by size:
3887 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5284 5471 1700
Size: 110 3474 187 2869
Fragments arranged by size:
3474 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2224 96
Size: 2128 4512
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6640
BssSI C'ACGA_G
Cuts at: 4700 5712 4700
Size: 1012 5628
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6640
BstBI TT'CG_AA
Cuts at: 5444 5444
Size: 6640
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6640
BstXI CCAn_nnnn'nTGG
Cuts at: 3595 3595
Size: 6640
BstYI r'GATC_y
Cuts at: 1972 2347 2555 2976 3140 3405 3533 3813 3970
Size: 375 208 421 164 265 128 280 157
Cuts at: 3970 4337 4722 4739 6191 6277 6289 6635 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 421 385 375 367 346 280
265 208 164 157 128 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2607 3225 2607
Size: 618 6022
BtgI C'CryG_G
Cuts at: 513 1065 3342 3645 513
Size: 552 2277 303 3508
Fragments arranged by size:
3508 2277 552 303
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5901
Fragments arranged by size:
5901 683 56
BtsI GCAGTG_nn'
Cuts at: 2119 4156 4950 4970 2119
Size: 2037 794 20 3789
Fragments arranged by size:
3789 2037 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6640
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2280 2315 529
Size: 35 1716 35 4854
Fragments arranged by size:
4854 1716 35 35
DraI TTT'AAA
Cuts at: 2730 2850 3195 3944 2730
Size: 120 345 749 5426
Fragments arranged by size:
5426 749 345 120
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5647 809
Size: 507 4331 1802
Fragments arranged by size:
4331 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2568 3585 3693
Size: 54 1425 50 275 18 738 1017 108
Cuts at: 3693 4087 4976 6605 8
Size: 394 889 1629 43
Fragments arranged by size:
1629 1425 1017 889 738 394 275 108
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4087 1537
Size: 275 2275 4090
Fragments arranged by size:
4090 2275 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3258 3279 3426 3462 3753 3882 4570 1353
Size: 1905 21 147 36 291 129 688 3423
Fragments arranged by size:
3423 1905 688 291 147 129 36 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5227 5601 5747 387
Size: 1064 138 3638 374 146 1280
Fragments arranged by size:
3638 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3038 3450 3672 3911 4703 5300 6087
Size: 393 1406 412 222 239 792 597 787
Cuts at: 6087 6355 1239
Size: 268 1524
Fragments arranged by size:
1524 1406 792 787 597 412 393 268
239 222
EcoRI G'AATT_C
Cuts at: 3949 4327 3949
Size: 378 6262
EcoRV GAT'ATC
Cuts at: 2771 3574 2771
Size: 803 5837
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3255 3437 3255
Size: 182 6458
FspI TGC'GCA
Cuts at: 5146 5146
Size: 6640
HaeII r_GCGC'y
Cuts at: 1678 5787 1678
Size: 4109 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2455 2468 2487 2500 2624
Size: 32 135 32 883 13 19 13 124
Cuts at: 2624 2636 2656 2668 3368 3400 5281 5313 5355
Size: 12 20 12 700 32 1881 32 42
Cuts at: 5355 5387 1373
Size: 32 2626
Fragments arranged by size:
2626 1881 883 700 135 124 42 32
32 32 32 32 20 19 13 13
12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2443 3984 4230 669
Size: 988 281 505 1541 246 3079
Fragments arranged by size:
3079 1541 988 505 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6640
HpaI GTT'AAC
Cuts at: 4230 4230
Size: 6640
KpnI G_GTAC'C
Cuts at: 3965 3965
Size: 6640
MfeI C'AATT_G
Cuts at: 3366 4239 3366
Size: 873 5767
MluI A'CGCG_T
Cuts at: 6516 6516
Size: 6640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2179 2622 5754 5938 1305
Size: 874 443 3132 184 2007
Fragments arranged by size:
3132 2007 874 443 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2570 3587 3695 10
Size: 54 1425 343 738 1017 108 2955
Fragments arranged by size:
2955 1425 1017 738 343 108 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2659 3644 3795 4598 4957 5116 518
Size: 648 1493 985 151 803 359 159 2042
Fragments arranged by size:
2042 1493 985 803 648 359 159 151
MspA1I CmG'CkG
Cuts at: 1606 2428 3160 4056 4733 5881 6126 1606
Size: 822 732 896 677 1148 245 2120
Fragments arranged by size:
2120 1148 896 822 732 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3342 3645 513
Size: 552 2277 303 3508
Fragments arranged by size:
3508 2277 552 303
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6640
NheI G'CTAG_C
Cuts at: 1052 3800 1052
Size: 2748 3892
NotI GC'GGCC_GC
Cuts at: 4087 4087
Size: 6640
NsiI A_TGCA'T
Cuts at: 2454 2666 2698 3451 2454
Size: 212 32 753 5643
Fragments arranged by size:
5643 753 212 32
NspI r_CATG'y
Cuts at: 1132 1229 2452 2664 2806 3661 3998 5543 1132
Size: 97 1223 212 142 855 337 1545 2229
Fragments arranged by size:
2229 1545 1223 855 337 212 142 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6640
PciI A'CATG_T
Cuts at: 3657 5539 3657
Size: 1882 4758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6640
PmeI GTTT'AAAC
Cuts at: 3944 3944
Size: 6640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3909 3941 4709 4741 6222 6254 3909
Size: 32 768 32 1481 32 4295
Fragments arranged by size:
4295 1481 768 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6640
PsiI TTA'TAA
Cuts at: 4210 4210
Size: 6640
PstI C_TGCA'G
Cuts at: 830 2988 3992 830
Size: 2158 1004 3478
Fragments arranged by size:
3478 2158 1004
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6640
PvuII CAG'CTG
Cuts at: 2428 3160 2428
Size: 732 5908
SalI G'TCGA_C
Cuts at: 1936 3982 1936
Size: 2046 4594
SapI GCTCTTCn'nnn_
Cuts at: 3882 3882
Size: 6640
SbfI CC_TGCA'GG
Cuts at: 3992 3992
Size: 6640
ScaI AGT'ACT
Cuts at: 2333 3437 4888 6600 2333
Size: 1104 1451 1712 2373
Fragments arranged by size:
2373 1712 1451 1104
SfcI C'TryA_G
Cuts at: 826 1045 2876 2984 3988 5123 5804 5995 826
Size: 219 1831 108 1004 1135 681 191 1471
Fragments arranged by size:
1831 1471 1135 1004 681 219 191 108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6640
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6640
SmaI CCC'GGG
Cuts at: 1872 3967 1872
Size: 2095 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 3776 3843 4744 5645 5907
Size: 197 926 240 1593 67 901 901 262
Cuts at: 5907 820
Size: 1553
Fragments arranged by size:
1593 1553 926 901 901 262 240 197
67
SnaBI TAC'GTA
Cuts at: 493 4431 493
Size: 3938 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6640
SphI G_CATG'C
Cuts at: 1132 2664 3998 1132
Size: 1532 1334 3774
Fragments arranged by size:
3774 1532 1334
SspI AAT'ATT
Cuts at: 5 52 4564 5
Size: 47 4512 2081
Fragments arranged by size:
4512 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6640
StyI C'CwwG_G
Cuts at: 513 1065 2623 3342 3391 3645 4079 513
Size: 552 1558 719 49 254 434 3074
Fragments arranged by size:
3074 1558 719 552 434 254 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2283 4667 4684 4837 5022 5467 325
Size: 1255 703 2384 17 153 185 445 1498
Fragments arranged by size:
2384 1498 1255 703 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2224 2331 3010
Size: 274 80 33 51 1443 247 107 679
Cuts at: 3010 3172 3435 3879 4886 6598 96
Size: 162 263 444 1007 1712 138
Fragments arranged by size:
1712 1443 1007 679 444 274 263 247
162 138 107 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2742 2774 6600 6632 2742
Size: 32 3826 32 2750
Fragments arranged by size:
3826 2750 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6640
XbaI T'CTAG_A
Cuts at: 3622 3976 3622
Size: 354 6286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3643 3736 3643
Size: 93 6547
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6640
XmaI C'CCGG_G
Cuts at: 1870 3965 1870
Size: 2095 4545
XmnI GAAnn'nnTTC
Cuts at: 3235 4769 3235
Size: 1534 5106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4448 276
Size: 53 83 186 936 2914 2468
Fragments arranged by size:
2914 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI