Restriction map for
pFN21AE0990
(Circular) (Six-base) MAPSORT of: pFN21AE0990.seq Check: 1400 from: 1 to: 6580
pFN21AE0990 6580 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3132 3132
Size: 6580
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2676 3137 4390 278
Size: 53 83 186 936 1140 461 1253 2468
Fragments arranged by size:
2468 1253 1140 936 461 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2676 3923 1937
Size: 739 1247 4594
Fragments arranged by size:
4594 1247 739
Acc65I G'GTAC_C
Cuts at: 3901 3901
Size: 6580
AclI AA'CG_TT
Cuts at: 1563 4707 5080 1563
Size: 3144 373 3063
Fragments arranged by size:
3144 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2496 2521 3184 3296 4643 6027 6295 1632
Size: 864 25 663 112 1347 1384 268 1917
Fragments arranged by size:
1917 1384 1347 864 663 268 112 25
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6383
AflIII A'CryG_T
Cuts at: 1203 5479 6456 1203
Size: 4276 977 1327
Fragments arranged by size:
4276 1327 977
AgeI A'CCGG_T
Cuts at: 5411 5411
Size: 6580
AhdI GACnn_n'nnGTC
Cuts at: 5309 5309
Size: 6580
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6580
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6580
AlwNI CAG_nnn'CTG
Cuts at: 2360 2949 3656 4323 5895 2360
Size: 589 707 667 1572 3045
Fragments arranged by size:
3045 1572 707 667 589
ApaLI G'TGCA_C
Cuts at: 4637 5793 4637
Size: 1156 5424
ApoI r'AATT_y
Cuts at: 1493 3297 3889 4115 4267 6376 1493
Size: 1804 592 226 152 2109 1697
Fragments arranged by size:
2109 1804 1697 592 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5134 160
Size: 872 4102 1606
Fragments arranged by size:
4102 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
AvaI C'yCGr_G
Cuts at: 1870 1943 3905 1870
Size: 73 1962 4545
Fragments arranged by size:
4545 1962 73
BamHI G'GATC_C
Cuts at: 3910 4277 3910
Size: 367 6213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3640 3901 5356 618
Size: 325 217 627 1853 261 1455 1842
Fragments arranged by size:
1853 1842 1455 627 325 261 217
BanII G_rGCy'C
Cuts at: 1391 2253 2449 3020 3870 3899 1391
Size: 862 196 571 850 29 4072
Fragments arranged by size:
4072 862 850 571 196 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2701 3122 3338 928
Size: 971 802 421 216 4170
Fragments arranged by size:
4170 971 802 421 216
BbvCI CC'TCA_GC
Cuts at: 2373 2373
Size: 6580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4771 4805 1903
Size: 34 2834 34 3678
Fragments arranged by size:
3678 2834 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4472 5682 4472
Size: 1210 5370
BclI T'GATC_A
Cuts at: 1548 2109 1548
Size: 561 6019
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5191 136
Size: 107 122 71 631 605 3519 1525
Fragments arranged by size:
3519 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3363 6575 3363
Size: 3212 3368
BlpI GC'TnA_GC
Cuts at: 2072 3997 2072
Size: 1925 4655
Bme1580I G_kGCm'C
Cuts at: 1790 2688 4641 5797 1790
Size: 898 1953 1156 2573
Fragments arranged by size:
2573 1953 1156 898
BmgBI CAC'GTC
Cuts at: 1318 2611 1318
Size: 1293 5287
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4311 5269 442
Size: 416 53 83 399 24 2894 958 1753
Fragments arranged by size:
2894 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6580
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 3742 2116
Size: 1626 4954
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 2421 2450 2601 2617 3011 3310 3401
Size: 1028 154 29 151 16 394 299 91
Cuts at: 3401 3773 5240 1239
Size: 372 1467 2579
Fragments arranged by size:
2579 1467 1028 394 372 299 154 151
91 29 16
Bpu10I CC'TnA_GC
Cuts at: 2373 2553 2953 2373
Size: 180 400 6000
Fragments arranged by size:
6000 400 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2518 4705 5570 5868 2518
Size: 2187 865 298 3230
Fragments arranged by size:
3230 2187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5243 882
Size: 617 3744 2219
Fragments arranged by size:
3744 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2107 4371 493
Size: 711 903 2264 2702
Fragments arranged by size:
2702 2264 903 711
BsaBI GATnn'nnATC
Cuts at: 6574 6574
Size: 6580
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2673 3134 4387 4769
Size: 53 83 186 936 1140 461 1253 382
Cuts at: 4769 5404 275
Size: 635 1451
Fragments arranged by size:
1451 1253 1140 936 635 461 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3129 3403 5013 5411 5685 5832 6243 3129
Size: 274 1610 398 274 147 411 3466
Fragments arranged by size:
3466 1610 411 398 274 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2171 2201 1178
Size: 30 165 30 768 30 5557
Fragments arranged by size:
5557 768 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3278 3613 3616 3718 3721 1186
Size: 2092 335 3 102 3 4045
Fragments arranged by size:
4045 2092 335 102 3 3
BseYI C'CCAG_C
Cuts at: 2124 2821 2934 5783 6279 2124
Size: 697 113 2849 496 2425
Fragments arranged by size:
2849 2425 697 496 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2177 1163
Size: 573 441 5566
Fragments arranged by size:
5566 573 441
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4030 4791 5819 1540
Size: 275 186 2029 761 1028 2301
Fragments arranged by size:
2301 2029 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2253 3020 3870 4641 4726 5797 2253
Size: 767 850 771 85 1071 3036
Fragments arranged by size:
3036 1071 850 771 767 85
BsmI GAATG_Cn'
Cuts at: 4091 4184 4091
Size: 93 6487
BsmBI CGTCTCn'nnnn_
Cuts at: 3518 3518
Size: 6580
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2253 2449 2688 3020 3870 3899 4641
Size: 399 463 196 239 332 850 29 742
Cuts at: 4641 4726 5797 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 850 742 463 399 332 239
196 85 29
BspEI T'CCGG_A
Cuts at: 3129 3403 6243 3129
Size: 274 2840 3466
Fragments arranged by size:
3466 2840 274
BspHI T'CATG_A
Cuts at: 4469 6199 4469
Size: 1730 4850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2988 3132 3935 844
Size: 2144 144 803 3489
Fragments arranged by size:
3489 2144 803 144
BsrBI CCG'CTC
Cuts at: 1394 1823 2152 3508 4467 1394
Size: 429 329 1356 959 3507
Fragments arranged by size:
3507 1356 959 429 329
BsrDI GCAATG_nn'
Cuts at: 65 1248 3252 5075 5249 65
Size: 1183 2004 1823 174 1396
Fragments arranged by size:
2004 1823 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2287 2455 5224 5411 1700
Size: 110 477 168 2769 187 2869
Fragments arranged by size:
2869 2769 477 187 168 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6580
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6580
BssSI C'ACGA_G
Cuts at: 4640 5652 4640
Size: 1012 5568
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3656 794
Size: 2862 3718
BstBI TT'CG_AA
Cuts at: 5384 5384
Size: 6580
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6580
BstXI CCAn_nnnn'nTGG
Cuts at: 3637 3637
Size: 6580
BstYI r'GATC_y
Cuts at: 1972 3363 3910 4277 4662 4679 6131 6217 6229
Size: 1391 547 367 385 17 1452 86 12
Cuts at: 6229 6575 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1391 547 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2145 3429 513
Size: 552 939 141 1284 3664
Fragments arranged by size:
3664 1284 939 552 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5841
Fragments arranged by size:
5841 683 56
BtsI GCAGTG_nn'
Cuts at: 4096 4890 4910 4096
Size: 794 20 5766
Fragments arranged by size:
5766 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3171 3206 529
Size: 35 2607 35 3903
Fragments arranged by size:
3903 2607 35 35
DraI TTT'AAA
Cuts at: 3884 3884
Size: 6580
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2901 5587 809
Size: 507 1585 2686 1802
Fragments arranged by size:
2686 1802 1585 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2409 3219 3426
Size: 54 1425 50 275 18 579 810 207
Cuts at: 3426 4027 4916 6545 8
Size: 601 889 1629 43
Fragments arranged by size:
1629 1425 889 810 601 579 275 207
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4027 1537
Size: 275 2215 4090
Fragments arranged by size:
4090 2215 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2382 3498 3571 4510 1353
Size: 1029 1116 73 939 3423
Fragments arranged by size:
3423 1116 1029 939 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5167 5541 5687 387
Size: 1064 138 3578 374 146 1280
Fragments arranged by size:
3578 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2251 3018 3868 2251
Size: 767 850 4963
Fragments arranged by size:
4963 850 767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2421 2450 2496 2521 2601 2617
Size: 393 635 154 29 46 25 80 16
Cuts at: 2617 3011 3184 3296 3310 3401 3773 4643 5240
Size: 394 173 112 14 91 372 870 597
Cuts at: 5240 6027 6295 1239
Size: 787 268 1524
Fragments arranged by size:
1524 870 787 635 597 394 393 372
268 173 154 112 91 80 46 29
25 16 14
EcoNI CCTnn'n_nnAGG
Cuts at: 3383 3473 3383
Size: 90 6490
EcoO109I rG'GnC_Cy
Cuts at: 2430 2661 3226 3421 2430
Size: 231 565 195 5589
Fragments arranged by size:
5589 565 231 195
EcoRI G'AATT_C
Cuts at: 3889 4267 3889
Size: 378 6202
FspI TGC'GCA
Cuts at: 5086 5086
Size: 6580
HaeII r_GCGC'y
Cuts at: 1678 5727 1678
Size: 4049 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3103 3135 3180 3212 3608
Size: 32 135 32 1531 32 45 32 396
Cuts at: 3608 3640 3716 3748 5221 5253 5295 5327 1373
Size: 32 76 32 1473 32 42 32 2626
Fragments arranged by size:
2626 1531 1473 396 135 76 45 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3088 3601 3924 4170 669
Size: 988 281 1150 513 323 246 3079
Fragments arranged by size:
3079 1150 988 513 323 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6580
HpaI GTT'AAC
Cuts at: 4170 4170
Size: 6580
KpnI G_GTAC'C
Cuts at: 3905 3905
Size: 6580
MfeI C'AATT_G
Cuts at: 4179 4179
Size: 6580
MluI A'CGCG_T
Cuts at: 6456 6456
Size: 6580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3597 5694 5878 1305
Size: 2292 2097 184 2007
Fragments arranged by size:
2292 2097 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2411 3428 10
Size: 54 1425 343 579 1017 3162
Fragments arranged by size:
3162 1425 1017 579 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2805 3110 3167 3635 4538 4897 5056
Size: 648 1639 305 57 468 903 359 159
Cuts at: 5056 518
Size: 2042
Fragments arranged by size:
2042 1639 903 648 468 359 305 159
57
MspA1I CmG'CkG
Cuts at: 1606 2026 2128 3178 3204 3996 4673 5821 6066
Size: 420 102 1050 26 792 677 1148 245
Cuts at: 6066 1606
Size: 2120
Fragments arranged by size:
2120 1148 1050 792 677 420 245 102
26
NaeI GCC'GGC
Cuts at: 2457 2457
Size: 6580
NcoI C'CATG_G
Cuts at: 513 1065 2004 2145 513
Size: 552 939 141 4948
Fragments arranged by size:
4948 939 552 141
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6580
NgoMIV G'CCGG_C
Cuts at: 2455 2455
Size: 6580
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6580
NotI GC'GGCC_GC
Cuts at: 4027 4027
Size: 6580
NspI r_CATG'y
Cuts at: 1132 1229 2203 3938 5483 1132
Size: 97 974 1735 1545 2229
Fragments arranged by size:
2229 1735 1545 974 97
PasI CC'CwG_GG
Cuts at: 1348 2426 3645 1348
Size: 1078 1219 4283
Fragments arranged by size:
4283 1219 1078
PciI A'CATG_T
Cuts at: 5479 5479
Size: 6580
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6580
PfoI T'CCnGG_A
Cuts at: 2743 3326 2743
Size: 583 5997
PmeI GTTT'AAAC
Cuts at: 3884 3884
Size: 6580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4649 4681 6162 6194 4649
Size: 32 1481 32 5035
Fragments arranged by size:
5035 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2430 2661 3226 2430
Size: 231 565 5784
Fragments arranged by size:
5784 565 231
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6580
PsiI TTA'TAA
Cuts at: 4150 4150
Size: 6580
PstI C_TGCA'G
Cuts at: 830 2901 3474 3932 830
Size: 2071 573 458 3478
Fragments arranged by size:
3478 2071 573 458
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6580
PvuII CAG'CTG
Cuts at: 2026 2128 3178 3204 2026
Size: 102 1050 26 5402
Fragments arranged by size:
5402 1050 102 26
SacI G_AGCT'C
Cuts at: 2253 3020 3870 2253
Size: 767 850 4963
Fragments arranged by size:
4963 850 767
SalI G'TCGA_C
Cuts at: 1936 3922 1936
Size: 1986 4594
SapI GCTCTTCn'nnn_
Cuts at: 3498 3571 3498
Size: 73 6507
SbfI CC_TGCA'GG
Cuts at: 3474 3932 3474
Size: 458 6122
ScaI AGT'ACT
Cuts at: 4828 6540 4828
Size: 1712 4868
SfcI C'TryA_G
Cuts at: 826 1045 2677 2885 2897 3470 3928 5063 5744
Size: 219 1632 208 12 573 458 1135 681
Cuts at: 5744 5935 826
Size: 191 1471
Fragments arranged by size:
1632 1471 1135 681 573 458 219 208
191 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6580
SmaI CCC'GGG
Cuts at: 1872 3907 1872
Size: 2035 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2497 4684 5585 5847 820
Size: 197 926 554 2187 901 262 1553
Fragments arranged by size:
2187 1553 926 901 554 262 197
SnaBI TAC'GTA
Cuts at: 493 4371 493
Size: 3878 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6580
SphI G_CATG'C
Cuts at: 1132 2203 3938 1132
Size: 1071 1735 3774
Fragments arranged by size:
3774 1735 1071
SspI AAT'ATT
Cuts at: 5 52 3829 4504 5
Size: 47 3777 675 2081
Fragments arranged by size:
3777 2081 675 47
StuI AGG'CCT
Cuts at: 1517 3740 1517
Size: 2223 4357
StyI C'CwwG_G
Cuts at: 513 1065 2004 2145 3536 4019 513
Size: 552 939 141 1391 483 3074
Fragments arranged by size:
3074 1391 939 552 483 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 3078 4607 4624 4777 4962 5407
Size: 1255 446 1052 1529 17 153 185 445
Cuts at: 5407 325
Size: 1498
Fragments arranged by size:
1529 1498 1255 1052 446 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4826 6538 96
Size: 274 80 33 51 1443 2849 1712 138
Fragments arranged by size:
2849 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 2171 2203 2222 2254 2533 2565 6540
Size: 32 103 32 19 32 279 32 3975
Cuts at: 6540 6572 2036
Size: 32 2044
Fragments arranged by size:
3975 2044 279 103 32 32 32 32
32 19
Tth111I GACn'n_nGTC
Cuts at: 1316 2612 1316
Size: 1296 5284
XbaI T'CTAG_A
Cuts at: 3916 3916
Size: 6580
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6580
XmaI C'CCGG_G
Cuts at: 1870 3905 1870
Size: 2035 4545
XmnI GAAnn'nnTTC
Cuts at: 2702 4709 2702
Size: 2007 4573
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2674 3135 4388 276
Size: 53 83 186 936 1140 461 1253 2468
Fragments arranged by size:
2468 1253 1140 936 461 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI