Restriction map for
pFN21AE0988
(Circular) (Six-base) MAPSORT of: pFN21AE0988.seq Check: 1409 from: 1 to: 6562
pFN21AE0988 6562 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2034 2437 2034
Size: 403 6159
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4372 278
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3905 1937
Size: 1968 4594
Acc65I G'GTAC_C
Cuts at: 2493 3883 2493
Size: 1390 5172
AclI AA'CG_TT
Cuts at: 1563 4689 5062 1563
Size: 3126 373 3063
Fragments arranged by size:
3126 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2741 2747 2774 3020 3137 4625 6009
Size: 464 645 6 27 246 117 1488 1384
Cuts at: 6009 6277 1632
Size: 268 1917
Fragments arranged by size:
1917 1488 1384 645 464 268 246 117
27 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6365
AflIII A'CryG_T
Cuts at: 1203 2510 5461 6438 1203
Size: 1307 2951 977 1327
Fragments arranged by size:
2951 1327 1307 977
AgeI A'CCGG_T
Cuts at: 5393 5393
Size: 6562
AhdI GACnn_n'nnGTC
Cuts at: 5291 5291
Size: 6562
AleI CACnn'nnGTG
Cuts at: 1166 2942 3691 1166
Size: 1776 749 4037
Fragments arranged by size:
4037 1776 749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2269 2582 3422 3547 1184
Size: 1085 313 840 125 4199
Fragments arranged by size:
4199 1085 840 313 125
AlwNI CAG_nnn'CTG
Cuts at: 2122 2798 4305 5877 2122
Size: 676 1507 1572 2807
Fragments arranged by size:
2807 1572 1507 676
ApaI G_GGCC'C
Cuts at: 3825 3825
Size: 6562
ApaLI G'TGCA_C
Cuts at: 2156 3377 4619 5775 2156
Size: 1221 1242 1156 2943
Fragments arranged by size:
2943 1242 1221 1156
ApoI r'AATT_y
Cuts at: 1493 3871 4097 4249 6358 1493
Size: 2378 226 152 2109 1697
Fragments arranged by size:
2378 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5116 160
Size: 872 4084 1606
Fragments arranged by size:
4084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
AvaI C'yCGr_G
Cuts at: 1870 1943 2327 2675 3887 1870
Size: 73 384 348 1212 4545
Fragments arranged by size:
4545 1212 384 348 73
BamHI G'GATC_C
Cuts at: 3892 4259 3892
Size: 367 6195
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2204 2493 2681 3464 3883
Size: 325 217 627 417 289 188 783 419
Cuts at: 3883 5338 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 783 627 419 417 325 289
217 188
BanII G_rGCy'C
Cuts at: 1391 2103 2253 2742 2957 2979 3825 3881 1391
Size: 712 150 489 215 22 846 56 4072
Fragments arranged by size:
4072 846 712 489 215 150 56 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2338 2627 928
Size: 971 439 289 4863
Fragments arranged by size:
4863 971 439 289
BbvCI CC'TCA_GC
Cuts at: 2570 3846 2570
Size: 1276 5286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4753 4787 1903
Size: 34 50 34 2732 34 3678
Fragments arranged by size:
3678 2732 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2870 4454 5664 2870
Size: 1584 1210 3768
Fragments arranged by size:
3768 1584 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6562
BfrBI ATG'CAT
Cuts at: 3534 3534
Size: 6562
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2795 5173 136
Size: 107 122 71 631 605 1123 2378 1525
Fragments arranged by size:
2378 1525 1123 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6557 6557
Size: 6562
BlpI GC'TnA_GC
Cuts at: 3493 3979 3493
Size: 486 6076
Bme1580I G_kGCm'C
Cuts at: 1790 2160 2594 2684 3128 3381 3686 3825 4623
Size: 370 434 90 444 253 305 139 798
Cuts at: 4623 5779 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 798 444 434 370 305 253
139 90
BmgBI CAC'GTC
Cuts at: 1318 2394 3100 1318
Size: 1076 706 4780
Fragments arranged by size:
4780 1076 706
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3470 4293 5251
Size: 416 53 83 399 24 2053 823 958
Cuts at: 5251 442
Size: 1753
Fragments arranged by size:
2053 1753 958 823 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6562
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2281 2281
Size: 6562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 2438 3380 3538 3592 5222 1239
Size: 1184 15 942 158 54 1630 2579
Fragments arranged by size:
2579 1630 1184 942 158 54 15
Bpu10I CC'TnA_GC
Cuts at: 2570 2886 3846 2570
Size: 316 960 5286
Fragments arranged by size:
5286 960 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2963 3497 4687 5552 5850 2963
Size: 534 1190 865 298 3675
Fragments arranged by size:
3675 1190 865 534 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5225 882
Size: 617 3726 2219
Fragments arranged by size:
3726 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2383 4353 493
Size: 711 1179 1970 2702
Fragments arranged by size:
2702 1970 1179 711
BsaBI GATnn'nnATC
Cuts at: 6556 6556
Size: 6562
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2364 3213 4369 4751
Size: 53 83 186 936 831 849 1156 382
Cuts at: 4751 5386 275
Size: 635 1451
Fragments arranged by size:
1451 1156 936 849 831 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4995 5393 5667 5814 6225 4995
Size: 398 274 147 411 5332
Fragments arranged by size:
5332 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3587 3617 1178
Size: 30 165 30 2184 30 4123
Fragments arranged by size:
4123 2184 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2531 2987 3005 3311 3377 1186
Size: 895 450 456 18 306 66 4371
Fragments arranged by size:
4371 895 456 450 306 66 18
BseYI C'CCAG_C
Cuts at: 2131 2815 3254 5765 6261 2131
Size: 684 439 2511 496 2432
Fragments arranged by size:
2511 2432 684 496 439
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3254 3314 1163
Size: 573 1518 60 4411
Fragments arranged by size:
4411 1518 573 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2318 2924 4012 4773 5801 1540
Size: 275 186 317 606 1088 761 1028 2301
Fragments arranged by size:
2301 1088 1028 761 606 317 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 2160 2253 2979 3381 4623 4708 5779 2073
Size: 87 93 726 402 1242 85 1071 2856
Fragments arranged by size:
2856 1242 1071 726 402 93 87 85
BsmI GAATG_Cn'
Cuts at: 2670 3536 4073 4166 2670
Size: 866 537 93 5066
Fragments arranged by size:
5066 866 537 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2355 2842 2355
Size: 487 6075
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2103 2160 2253 2594 2684 2742
Size: 399 283 30 57 93 341 90 58
Cuts at: 2742 2957 2979 3128 3381 3686 3825 3881 4623
Size: 215 22 149 253 305 139 56 742
Cuts at: 4623 4708 5779 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 341 305 283 253
215 149 139 93 90 85 58 57
56 30 22
BspEI T'CCGG_A
Cuts at: 6225 6225
Size: 6562
BspHI T'CATG_A
Cuts at: 3717 4451 6181 3717
Size: 734 1730 4098
Fragments arranged by size:
4098 1730 734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2034 2304 2437 3917 844
Size: 1190 270 133 1480 3489
Fragments arranged by size:
3489 1480 1190 270 133
BsrBI CCG'CTC
Cuts at: 1394 1823 2482 3492 3511 4449 1394
Size: 429 659 1010 19 938 3507
Fragments arranged by size:
3507 1010 938 659 429 19
BsrDI GCAATG_nn'
Cuts at: 65 1248 2214 2460 3319 5057 5231 65
Size: 1183 966 246 859 1738 174 1396
Fragments arranged by size:
1738 1396 1183 966 859 246 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2219 2242 2313 3417 5206 5393 1700
Size: 110 409 23 71 1104 1789 187 2869
Fragments arranged by size:
2869 1789 1104 409 187 110 71 23
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6562
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6562
BssSI C'ACGA_G
Cuts at: 2153 2477 2938 4622 5634 2153
Size: 324 461 1684 1012 3081
Fragments arranged by size:
3081 1684 1012 461 324
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2164 2307 2667 3172 3464 3781 794
Size: 1370 143 360 505 292 317 3575
Fragments arranged by size:
3575 1370 505 360 317 292 143
BstBI TT'CG_AA
Cuts at: 5366 5366
Size: 6562
BstEII G'GTnAC_C
Cuts at: 1184 3583 1184
Size: 2399 4163
BstXI CCAn_nnnn'nTGG
Cuts at: 3460 3814 3460
Size: 354 6208
BstYI r'GATC_y
Cuts at: 1972 3300 3732 3892 4259 4644 4661 6113 6199
Size: 1328 432 160 367 385 17 1452 86
Cuts at: 6199 6211 6557 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1328 432 385 367 346 160
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2791 2791
Size: 6562
BtgI C'CryG_G
Cuts at: 513 1065 2016 2367 2576 2918 2931 3342 3768
Size: 552 951 351 209 342 13 411 426
Cuts at: 3768 513
Size: 3307
Fragments arranged by size:
3307 951 552 426 411 351 342 209
13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3579 487
Size: 683 56 2353 3470
Fragments arranged by size:
3470 2353 683 56
BtsI GCAGTG_nn'
Cuts at: 2782 3483 4078 4872 4892 2782
Size: 701 595 794 20 4452
Fragments arranged by size:
4452 794 701 595 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6527
DraI TTT'AAA
Cuts at: 3866 3866
Size: 6562
DraIII CAC_nnn'GTG
Cuts at: 2164 3337 3464 3591 2164
Size: 1173 127 127 5135
Fragments arranged by size:
5135 1173 127 127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5569 809
Size: 507 4253 1802
Fragments arranged by size:
4253 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2315 2921 3102
Size: 54 1425 50 275 18 485 606 181
Cuts at: 3102 3318 3506 3610 3804 4009 4898 6527 8
Size: 216 188 104 194 205 889 1629 43
Fragments arranged by size:
1629 1425 889 606 485 275 216 205
194 188 181 104 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2315 2921 4009 1537
Size: 275 503 606 1088 4090
Fragments arranged by size:
4090 1088 606 503 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 2999 3141 4492 1353
Size: 755 891 142 1351 3423
Fragments arranged by size:
3423 1351 891 755 142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2108 2747 5149 5523 5669 387
Size: 1064 138 519 639 2402 374 146 1280
Fragments arranged by size:
2402 1280 1064 639 519 374 146 138
EcoICRI GAG'CTC
Cuts at: 2251 2977 2251
Size: 726 5836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2423 2438 2741 2747 2774 3020
Size: 393 464 327 15 303 6 27 246
Cuts at: 3020 3137 3380 3538 3592 4625 5222 6009 6277
Size: 117 243 158 54 1033 597 787 268
Cuts at: 6277 1239
Size: 1524
Fragments arranged by size:
1524 1033 787 597 464 393 327 303
268 246 243 158 117 54 27 15
6
EcoNI CCTnn'n_nnAGG
Cuts at: 2420 2756 2420
Size: 336 6226
EcoO109I rG'GnC_Cy
Cuts at: 2413 2440 3229 3821 3822 2413
Size: 27 789 592 1 5153
Fragments arranged by size:
5153 789 592 27 1
EcoRI G'AATT_C
Cuts at: 3871 4249 3871
Size: 378 6184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3010 3010
Size: 6562
FspI TGC'GCA
Cuts at: 2300 3083 5068 2300
Size: 783 1985 3794
Fragments arranged by size:
3794 1985 783
HaeII r_GCGC'y
Cuts at: 1678 2194 5709 1678
Size: 516 3515 2531
Fragments arranged by size:
3515 2531 516
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2827 2859 3587 3619 5203
Size: 32 135 32 1255 32 728 32 1584
Cuts at: 5203 5235 5277 5309 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1584 1255 728 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2835 3415 3906 4152 669
Size: 988 281 897 580 491 246 3079
Fragments arranged by size:
3079 988 897 580 491 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6562
HpaI GTT'AAC
Cuts at: 3415 4152 3415
Size: 737 5825
KpnI G_GTAC'C
Cuts at: 2497 3887 2497
Size: 1390 5172
MfeI C'AATT_G
Cuts at: 4161 4161
Size: 6562
MluI A'CGCG_T
Cuts at: 2510 6438 2510
Size: 3928 2634
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5676 5860 1305
Size: 4371 184 2007
Fragments arranged by size:
4371 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3104 3806 10
Size: 54 1425 343 1272 702 2766
Fragments arranged by size:
2766 1425 1272 702 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2942 3110 3248 3347 3529 3691 4520
Size: 648 1776 168 138 99 182 162 829
Cuts at: 4520 4879 5038 518
Size: 359 159 2042
Fragments arranged by size:
2042 1776 829 648 359 182 168 162
159 138 99
MspA1I CmG'CkG
Cuts at: 1606 2018 2578 2707 2920 3181 3268 3978 4655
Size: 412 560 129 213 261 87 710 677
Cuts at: 4655 5803 6048 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 710 677 560 412 261 245
213 129 87
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 6562
NcoI C'CATG_G
Cuts at: 513 1065 2367 2931 3342 513
Size: 552 1302 564 411 3733
Fragments arranged by size:
3733 1302 564 552 411
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6562
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 6562
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6562
NotI GC'GGCC_GC
Cuts at: 4009 4009
Size: 6562
NsiI A_TGCA'T
Cuts at: 3536 3536
Size: 6562
NspI r_CATG'y
Cuts at: 1132 1229 3082 3784 3920 5465 1132
Size: 97 1853 702 136 1545 2229
Fragments arranged by size:
2229 1853 1545 702 136 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6562
PciI A'CATG_T
Cuts at: 5461 5461
Size: 6562
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6562
PmeI GTTT'AAAC
Cuts at: 3866 3866
Size: 6562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4663 6144 6176 4631
Size: 32 1481 32 5017
Fragments arranged by size:
5017 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6562
PsiI TTA'TAA
Cuts at: 4132 4132
Size: 6562
PspOMI G'GGCC_C
Cuts at: 3821 3821
Size: 6562
PspXI vC'TCGA_Gb
Cuts at: 2327 2327
Size: 6562
PstI C_TGCA'G
Cuts at: 830 3914 830
Size: 3084 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6562
PvuII CAG'CTG
Cuts at: 2707 3181 2707
Size: 474 6088
SacI G_AGCT'C
Cuts at: 2253 2979 2253
Size: 726 5836
SacII CC_GC'GG
Cuts at: 2019 2579 2921 2019
Size: 560 342 5660
Fragments arranged by size:
5660 560 342
SalI G'TCGA_C
Cuts at: 1936 3904 1936
Size: 1968 4594
SapI GCTCTTCn'nnn_
Cuts at: 2108 2999 2108
Size: 891 5671
SbfI CC_TGCA'GG
Cuts at: 3914 3914
Size: 6562
ScaI AGT'ACT
Cuts at: 2666 4810 6522 2666
Size: 2144 1712 2706
Fragments arranged by size:
2706 2144 1712
SexAI A'CCwGG_T
Cuts at: 3333 3333
Size: 6562
SfcI C'TryA_G
Cuts at: 826 1045 2959 3796 3910 5045 5726 5917 826
Size: 219 1914 837 114 1135 681 191 1471
Fragments arranged by size:
1914 1471 1135 837 681 219 191 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6562
SmaI CCC'GGG
Cuts at: 1872 3889 1872
Size: 2017 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2327 2978 3512 4666 5567 5829
Size: 197 926 384 651 534 1154 901 262
Cuts at: 5829 820
Size: 1553
Fragments arranged by size:
1553 1154 926 901 651 534 384 262
197
SnaBI TAC'GTA
Cuts at: 493 4353 493
Size: 3860 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6562
SphI G_CATG'C
Cuts at: 1132 3082 3784 3920 1132
Size: 1950 702 136 3774
Fragments arranged by size:
3774 1950 702 136
SspI AAT'ATT
Cuts at: 5 52 4486 5
Size: 47 4434 2081
Fragments arranged by size:
4434 2081 47
StuI AGG'CCT
Cuts at: 1517 2762 3599 1517
Size: 1245 837 4480
Fragments arranged by size:
4480 1245 837
StyI C'CwwG_G
Cuts at: 513 1065 2367 2931 3342 4001 513
Size: 552 1302 564 411 659 3074
Fragments arranged by size:
3074 1302 659 564 552 411
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2567 2838 3452 4589 4606 4759 4944
Size: 1255 987 271 614 1137 17 153 185
Cuts at: 4944 5389 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1137 987 614 445 271 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2664 4808 6520
Size: 274 80 33 51 1443 687 2144 1712
Cuts at: 6520 96
Size: 138
Fragments arranged by size:
2144 1712 1443 687 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3323 3355 6522 6554 3323
Size: 32 3167 32 3331
Fragments arranged by size:
3331 3167 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6562
XbaI T'CTAG_A
Cuts at: 3898 3898
Size: 6562
XhoI C'TCGA_G
Cuts at: 1943 2327 1943
Size: 384 6178
XmaI C'CCGG_G
Cuts at: 1870 3887 1870
Size: 2017 4545
XmnI GAAnn'nnTTC
Cuts at: 2993 4691 2993
Size: 1698 4864
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4370 276
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
EcoRV FseI FspAI KasI NarI NruI PacI PfoI
PmlI PpuMI PsrI RsrII SanDI SfiI SfoI SrfI
SwaI XcmI