Restriction map for
pFN21AE0987
(Circular) (Six-base) MAPSORT of: pFN21AE0987.seq Check: 9504 from: 1 to: 6553
pFN21AE0987 6553 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2350 3487 2350
Size: 1137 5416
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4363 278
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3896 1937
Size: 1959 4594
Acc65I G'GTAC_C
Cuts at: 3874 3874
Size: 6553
AclI AA'CG_TT
Cuts at: 1563 4680 5053 1563
Size: 3117 373 3063
Fragments arranged by size:
3117 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2192 2226 2443 3490 4616 6000 6268 1632
Size: 560 34 217 1047 1126 1384 268 1917
Fragments arranged by size:
1917 1384 1126 1047 560 268 217 34
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6356
AflIII A'CryG_T
Cuts at: 1203 2595 5452 6429 1203
Size: 1392 2857 977 1327
Fragments arranged by size:
2857 1392 1327 977
AgeI A'CCGG_T
Cuts at: 5384 5384
Size: 6553
AhdI GACnn_n'nnGTC
Cuts at: 2599 5282 2599
Size: 2683 3870
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6553
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2870 1184
Size: 1686 4867
AlwNI CAG_nnn'CTG
Cuts at: 2701 3058 3555 3570 3683 3705 4296 5868 2701
Size: 357 497 15 113 22 591 1572 3386
Fragments arranged by size:
3386 1572 591 497 357 113 22 15
ApaI G_GGCC'C
Cuts at: 2554 3416 3642 3761 2554
Size: 862 226 119 5346
Fragments arranged by size:
5346 862 226 119
ApaLI G'TGCA_C
Cuts at: 4610 5766 4610
Size: 1156 5397
ApoI r'AATT_y
Cuts at: 1493 3862 4088 4240 6349 1493
Size: 2369 226 152 2109 1697
Fragments arranged by size:
2369 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5107 160
Size: 872 4075 1606
Fragments arranged by size:
4075 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
AvaI C'yCGr_G
Cuts at: 1870 1943 3409 3590 3810 3878 1870
Size: 73 1466 181 220 68 4545
Fragments arranged by size:
4545 1466 220 181 73 68
BamHI G'GATC_C
Cuts at: 2483 3883 4250 2483
Size: 1400 367 4786
Fragments arranged by size:
4786 1400 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3259 3846 3874 5329 618
Size: 325 217 627 1472 587 28 1455 1842
Fragments arranged by size:
1842 1472 1455 627 587 325 217 28
BanII G_rGCy'C
Cuts at: 1391 2554 3416 3423 3642 3690 3761 3769 3777
Size: 1163 862 7 219 48 71 8 8
Cuts at: 3777 3872 1391
Size: 95 4072
Fragments arranged by size:
4072 1163 862 219 95 71 48 8
8 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5582
BbvCI CC'TCA_GC
Cuts at: 3192 3192
Size: 6553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4744 4778 1903
Size: 34 2807 34 3678
Fragments arranged by size:
3678 2807 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4445 5655 4445
Size: 1210 5343
BclI T'GATC_A
Cuts at: 1548 2973 1548
Size: 1425 5128
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2735 3453 5164
Size: 107 122 71 631 605 1063 718 1711
Cuts at: 5164 136
Size: 1525
Fragments arranged by size:
1711 1525 1063 718 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6548 6548
Size: 6553
BlpI GC'TnA_GC
Cuts at: 2622 3524 3603 3970 2622
Size: 902 79 367 5205
Fragments arranged by size:
5205 902 367 79
Bme1580I G_kGCm'C
Cuts at: 1790 2554 3070 3405 3416 3642 3761 3849 4614
Size: 764 516 335 11 226 119 88 765
Cuts at: 4614 5770 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 765 764 516 335 226 119
88 11
BmgBI CAC'GTC
Cuts at: 1318 2218 2596 1318
Size: 900 378 5275
Fragments arranged by size:
5275 900 378
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3012 4284 5242
Size: 416 53 83 399 24 1595 1272 958
Cuts at: 5242 442
Size: 1753
Fragments arranged by size:
1753 1595 1272 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6553
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3605
Size: 6553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 3732 3764 3815 5213 1239
Size: 1061 1432 32 51 1398 2579
Fragments arranged by size:
2579 1432 1398 1061 51 32
Bpu10I CC'TnA_GC
Cuts at: 3192 3417 3684 3768 3192
Size: 225 267 84 5977
Fragments arranged by size:
5977 267 225 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2114 2237 2252 2369 2399 2777 2843 2939 3359
Size: 123 15 117 30 378 66 96 420
Cuts at: 3359 4678 5543 5841 2114
Size: 1319 865 298 2826
Fragments arranged by size:
2826 1319 865 420 378 298 123 117
96 66 30 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3068 3329 3358 3538 5216 882
Size: 617 1569 261 29 180 1678 2219
Fragments arranged by size:
2219 1678 1569 617 261 180 29
BsaAI yAC'GTr
Cuts at: 493 1204 2515 4344 493
Size: 711 1311 1829 2702
Fragments arranged by size:
2702 1829 1311 711
BsaBI GATnn'nnATC
Cuts at: 6547 6547
Size: 6553
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2139 2247 4360 4742
Size: 53 83 186 936 606 108 2113 382
Cuts at: 4742 5377 275
Size: 635 1451
Fragments arranged by size:
2113 1451 936 635 606 382 186 108
83 53
BsaWI w'CCGG_w
Cuts at: 4986 5384 5658 5805 6216 4986
Size: 398 274 147 411 5323
Fragments arranged by size:
5323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6328
Fragments arranged by size:
6328 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3209 3266 1186
Size: 2023 57 4473
Fragments arranged by size:
4473 2023 57
BseYI C'CCAG_C
Cuts at: 2566 3314 3570 3698 5756 6252 2566
Size: 748 256 128 2058 496 2867
Fragments arranged by size:
2867 2058 748 496 256 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2165 2612 3325 3721 1163
Size: 573 429 447 713 396 3995
Fragments arranged by size:
3995 713 573 447 429 396
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4003 4764 5792 1540
Size: 275 186 2002 761 1028 2301
Fragments arranged by size:
2301 2002 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2102 2367 2946 3690 4614 4699 5770 2102
Size: 265 579 744 924 85 1071 2885
Fragments arranged by size:
2885 1071 924 744 579 265 85
BsmI GAATG_Cn'
Cuts at: 4064 4157 4064
Size: 93 6460
BsmBI CGTCTCn'nnnn_
Cuts at: 2813 2813
Size: 6553
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2367 2554 2946 3070 3405 3416
Size: 399 312 265 187 392 124 335 11
Cuts at: 3416 3423 3642 3690 3761 3769 3777 3849 3872
Size: 7 219 48 71 8 8 72 23
Cuts at: 3872 4614 4699 5770 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 392 335 312 265
219 187 124 85 72 71 48 23
11 8 8 7
BspEI T'CCGG_A
Cuts at: 6216 6216
Size: 6553
BspHI T'CATG_A
Cuts at: 4442 6172 4442
Size: 1730 4823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2350 3347 3487 3908 844
Size: 1506 997 140 421 3489
Fragments arranged by size:
3489 1506 997 421 140
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 4440 1394
Size: 429 1966 651 3507
Fragments arranged by size:
3507 1966 651 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5048 5222 65
Size: 1183 3800 174 1396
Fragments arranged by size:
3800 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3227 5197 5384 1700
Size: 110 1417 1970 187 2869
Fragments arranged by size:
2869 1970 1417 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6553
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6553
BssSI C'ACGA_G
Cuts at: 3071 4613 5625 3071
Size: 1542 1012 3999
Fragments arranged by size:
3999 1542 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2188 3058 794
Size: 1394 870 4289
Fragments arranged by size:
4289 1394 870
BstBI TT'CG_AA
Cuts at: 5357 5357
Size: 6553
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6553
BstXI CCAn_nnnn'nTGG
Cuts at: 3800 3800
Size: 6553
BstYI r'GATC_y
Cuts at: 1972 2124 2483 2559 3883 4250 4635 4652 6104
Size: 152 359 76 1324 367 385 17 1452
Cuts at: 6104 6190 6202 6548 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1324 385 367 359 346 152
86 76 17 12
Bsu36I CC'TnA_GG
Cuts at: 3540 3540
Size: 6553
BtgI C'CryG_G
Cuts at: 513 1065 3660 3761 513
Size: 552 2595 101 3305
Fragments arranged by size:
3305 2595 552 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5814
Fragments arranged by size:
5814 683 56
BtsI GCAGTG_nn'
Cuts at: 2182 2902 3070 3226 4069 4863 4883 2182
Size: 720 168 156 843 794 20 3852
Fragments arranged by size:
3852 843 794 720 168 156 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6553
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2700 2735 529
Size: 35 2136 35 4347
Fragments arranged by size:
4347 2136 35 35
DraI TTT'AAA
Cuts at: 3857 3857
Size: 6553
DraIII CAC_nnn'GTG
Cuts at: 3400 3400
Size: 6553
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2995 3365 5560 809
Size: 507 1679 370 2195 1802
Fragments arranged by size:
2195 1802 1679 507 370
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2814 2901
Size: 54 1425 50 275 18 285 699 87
Cuts at: 2901 3156 3225 3391 3612 4000 4889 6518 8
Size: 255 69 166 221 388 889 1629 43
Fragments arranged by size:
1629 1425 889 699 388 285 275 255
221 166 87 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4000 1537
Size: 275 2188 4090
Fragments arranged by size:
4090 2188 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2202 3695 4483 1353
Size: 849 1493 788 3423
Fragments arranged by size:
3423 1493 849 788
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2110 2476 2954 5140 5514 5660
Size: 1064 138 521 366 478 2186 374 146
Cuts at: 5660 387
Size: 1280
Fragments arranged by size:
2186 1280 1064 521 478 374 366 146
138
EcoICRI GAG'CTC
Cuts at: 3688 3688
Size: 6553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2226 2300 2443 3490 3732 3764
Size: 393 560 34 74 143 1047 242 32
Cuts at: 3764 3815 4616 5213 6000 6268 1239
Size: 51 801 597 787 268 1524
Fragments arranged by size:
1524 1047 801 787 597 560 393 268
242 143 74 51 34 32
EcoO109I rG'GnC_Cy
Cuts at: 2064 2551 2698 3171 3413 3552 3594 3757 2064
Size: 487 147 473 242 139 42 163 4860
Fragments arranged by size:
4860 487 473 242 163 147 139 42
EcoRI G'AATT_C
Cuts at: 3862 4240 3862
Size: 378 6175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 6553
FspI TGC'GCA
Cuts at: 3062 3104 5059 3062
Size: 42 1955 4556
Fragments arranged by size:
4556 1955 42
HaeII r_GCGC'y
Cuts at: 1678 2845 2936 5700 1678
Size: 1167 91 2764 2531
Fragments arranged by size:
2764 2531 1167 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 2585 2617 5194
Size: 32 135 32 896 32 85 32 2577
Cuts at: 5194 5226 5268 5300 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2577 896 135 85 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3897 4143 669
Size: 988 281 1959 246 3079
Fragments arranged by size:
3079 1959 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6553
HpaI GTT'AAC
Cuts at: 4143 4143
Size: 6553
KpnI G_GTAC'C
Cuts at: 3878 3878
Size: 6553
MfeI C'AATT_G
Cuts at: 4152 4152
Size: 6553
MluI A'CGCG_T
Cuts at: 6429 6429
Size: 6553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2001 2847 3381 3519 3525 5667 5851 1305
Size: 696 846 534 138 6 2142 184 2007
Fragments arranged by size:
2142 2007 846 696 534 184 138 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 2903 3393 3614 10
Size: 54 1425 343 285 786 490 221 2949
Fragments arranged by size:
2949 1425 786 490 343 285 221 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 3659 3798 4511 4870 5029 518
Size: 648 1269 1224 139 713 359 159 2042
Fragments arranged by size:
2042 1269 1224 713 648 359 159 139
MspA1I CmG'CkG
Cuts at: 1606 2451 3058 3289 3570 3705 3969 4646 5794
Size: 845 607 231 281 135 264 677 1148
Cuts at: 5794 6039 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 845 677 607 281 264 245
231 135
NaeI GCC'GGC
Cuts at: 3229 3229
Size: 6553
NcoI C'CATG_G
Cuts at: 513 1065 3660 513
Size: 552 2595 3406
Fragments arranged by size:
3406 2595 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6553
NgoMIV G'CCGG_C
Cuts at: 3227 3227
Size: 6553
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6553
NotI GC'GGCC_GC
Cuts at: 4000 4000
Size: 6553
NspI r_CATG'y
Cuts at: 1132 1229 2191 2986 3839 3911 5456 1132
Size: 97 962 795 853 72 1545 2229
Fragments arranged by size:
2229 1545 962 853 795 97 72
PasI CC'CwG_GG
Cuts at: 1348 2605 2695 1348
Size: 1257 90 5206
Fragments arranged by size:
5206 1257 90
PciI A'CATG_T
Cuts at: 5452 5452
Size: 6553
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2088 2784 1108
Size: 980 696 4877
Fragments arranged by size:
4877 980 696
PfoI T'CCnGG_A
Cuts at: 3709 3709
Size: 6553
PmeI GTTT'AAAC
Cuts at: 3857 3857
Size: 6553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4622 4654 6135 6167 4622
Size: 32 1481 32 5008
Fragments arranged by size:
5008 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2064 3171 2064
Size: 1107 5446
PshAI GACnn'nnGTC
Cuts at: 1613 3300 1613
Size: 1687 4866
PsiI TTA'TAA
Cuts at: 4123 4123
Size: 6553
PspOMI G'GGCC_C
Cuts at: 2550 3412 3638 3757 2550
Size: 862 226 119 5346
Fragments arranged by size:
5346 862 226 119
PstI C_TGCA'G
Cuts at: 830 2361 3309 3624 3905 830
Size: 1531 948 315 281 3478
Fragments arranged by size:
3478 1531 948 315 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6553
PvuII CAG'CTG
Cuts at: 3058 3289 3570 3705 3058
Size: 231 281 135 5906
Fragments arranged by size:
5906 281 231 135
SacI G_AGCT'C
Cuts at: 3690 3690
Size: 6553
SalI G'TCGA_C
Cuts at: 1936 3895 1936
Size: 1959 4594
SanDI GG'GwC_CC
Cuts at: 3171 3171
Size: 6553
SapI GCTCTTCn'nnn_
Cuts at: 3695 3695
Size: 6553
SbfI CC_TGCA'GG
Cuts at: 2361 3905 2361
Size: 1544 5009
ScaI AGT'ACT
Cuts at: 4801 6513 4801
Size: 1712 4841
SexAI A'CCwGG_T
Cuts at: 2262 2670 3262 2262
Size: 408 592 5553
Fragments arranged by size:
5553 592 408
SfcI C'TryA_G
Cuts at: 826 1045 2357 3305 3436 3620 3901 5036 5717
Size: 219 1312 948 131 184 281 1135 681
Cuts at: 5717 5908 826
Size: 191 1471
Fragments arranged by size:
1471 1312 1135 948 681 281 219 191
184 131
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6553
SmaI CCC'GGG
Cuts at: 1872 3812 3880 1872
Size: 1940 68 4545
Fragments arranged by size:
4545 1940 68
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2129 2231 2252 2384 2414 2792
Size: 197 926 186 102 21 132 30 378
Cuts at: 2792 2858 2954 3374 4657 5558 5820 820
Size: 66 96 420 1283 901 262 1553
Fragments arranged by size:
1553 1283 926 901 420 378 262 197
186 132 102 96 66 30 21
SnaBI TAC'GTA
Cuts at: 493 4344 493
Size: 3851 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6553
SphI G_CATG'C
Cuts at: 1132 2191 2986 3911 1132
Size: 1059 795 925 3774
Fragments arranged by size:
3774 1059 925 795
SspI AAT'ATT
Cuts at: 5 52 4477 5
Size: 47 4425 2081
Fragments arranged by size:
4425 2081 47
StuI AGG'CCT
Cuts at: 1517 2196 1517
Size: 679 5874
StyI C'CwwG_G
Cuts at: 513 1065 2082 3660 3992 513
Size: 552 1017 1578 332 3074
Fragments arranged by size:
3074 1578 1017 552 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3095 4580 4597 4750 4935 5380 325
Size: 1255 1515 1485 17 153 185 445 1498
Fragments arranged by size:
1515 1498 1485 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4799 6511 96
Size: 274 80 33 51 1443 2822 1712 138
Fragments arranged by size:
2822 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3600 3632 6513 6545 3600
Size: 32 2881 32 3608
Fragments arranged by size:
3608 2881 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6553
XbaI T'CTAG_A
Cuts at: 3889 3889
Size: 6553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3151 3286 3151
Size: 135 6418
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6553
XmaI C'CCGG_G
Cuts at: 1870 3810 3878 1870
Size: 1940 68 4545
Fragments arranged by size:
4545 1940 68
XmnI GAAnn'nnTTC
Cuts at: 2315 4682 2315
Size: 2367 4186
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4361 276
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoNI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI
SfoI SrfI SwaI