Restriction map for
pFN21AE0986
(Circular) (Six-base) MAPSORT of: pFN21AE0986.seq Check: 1838 from: 1 to: 6508
pFN21AE0986 6508 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2244 2629 2698 2829 2244
Size: 385 69 131 5923
Fragments arranged by size:
5923 385 131 69
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4318 278
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3141 3851 1937
Size: 1204 710 4594
Fragments arranged by size:
4594 1204 710
Acc65I G'GTAC_C
Cuts at: 3829 3829
Size: 6508
AclI AA'CG_TT
Cuts at: 1563 4635 5008 1563
Size: 3072 373 3063
Fragments arranged by size:
3072 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 2947 3524 4571 5955 6223 1632
Size: 437 878 577 1047 1384 268 1917
Fragments arranged by size:
1917 1384 1047 878 577 437 268
AfeI AGC'GCT
Cuts at: 2777 2777
Size: 6508
AflII C'TTAA_G
Cuts at: 820 1017 3050 820
Size: 197 2033 4278
Fragments arranged by size:
4278 2033 197
AflIII A'CryG_T
Cuts at: 1203 3144 5407 6384 1203
Size: 1941 2263 977 1327
Fragments arranged by size:
2263 1941 1327 977
AgeI A'CCGG_T
Cuts at: 3674 5339 3674
Size: 1665 4843
AhdI GACnn_n'nnGTC
Cuts at: 5237 5237
Size: 6508
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6508
AlwNI CAG_nnn'CTG
Cuts at: 3337 4251 5823 3337
Size: 914 1572 4022
Fragments arranged by size:
4022 1572 914
ApaI G_GGCC'C
Cuts at: 2598 3473 2598
Size: 875 5633
ApaLI G'TGCA_C
Cuts at: 4565 5721 4565
Size: 1156 5352
ApoI r'AATT_y
Cuts at: 1493 2872 2951 3364 3817 4043 4195 6304 1493
Size: 1379 79 413 453 226 152 2109 1697
Fragments arranged by size:
2109 1697 1379 453 413 226 152 79
AseI AT'TA_AT
Cuts at: 160 1032 5062 160
Size: 872 4030 1606
Fragments arranged by size:
4030 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 3056 3833 1870
Size: 73 152 961 777 4545
Fragments arranged by size:
4545 961 777 152 73
BamHI G'GATC_C
Cuts at: 3559 3838 4205 3559
Size: 279 367 5862
Fragments arranged by size:
5862 367 279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2368 2641 3597 3829 5284
Size: 325 217 627 581 273 956 232 1455
Cuts at: 5284 618
Size: 1842
Fragments arranged by size:
1842 1455 956 627 581 325 273 232
217
BanII G_rGCy'C
Cuts at: 1391 2103 2414 2598 2937 3175 3249 3377 3473
Size: 712 311 184 339 238 74 128 96
Cuts at: 3473 3827 1391
Size: 354 4072
Fragments arranged by size:
4072 712 354 339 311 238 184 128
96 74
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5537
BbvCI CC'TCA_GC
Cuts at: 3608 3608
Size: 6508
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4699 4733 1903
Size: 34 2762 34 3678
Fragments arranged by size:
3678 2762 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4400 5610 4400
Size: 1210 5298
BclI T'GATC_A
Cuts at: 1548 2230 1548
Size: 682 5826
BfrBI ATG'CAT
Cuts at: 3347 3347
Size: 6508
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2377 5119 136
Size: 107 122 71 631 605 705 2742 1525
Fragments arranged by size:
2742 1525 705 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6503 6503
Size: 6508
BlpI GC'TnA_GC
Cuts at: 2915 3718 3925 2915
Size: 803 207 5498
Fragments arranged by size:
5498 803 207
Bme1580I G_kGCm'C
Cuts at: 1790 2598 2646 3473 4569 5725 1790
Size: 808 48 827 1096 1156 2573
Fragments arranged by size:
2573 1156 1096 827 808 48
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6508
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4239 5197 442
Size: 416 53 83 399 24 2822 958 1753
Fragments arranged by size:
2822 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2888 3100 3159 5168 1239
Size: 1649 212 59 2009 2579
Fragments arranged by size:
2579 2009 1649 212 59
Bpu10I CC'TnA_GC
Cuts at: 3608 3608
Size: 6508
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2663 4633 5498 5796 2663
Size: 1970 865 298 3375
Fragments arranged by size:
3375 1970 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5171 882
Size: 617 3672 2219
Fragments arranged by size:
3672 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2656 2746 3147 4299 493
Size: 711 1452 90 401 1152 2702
Fragments arranged by size:
2702 1452 1152 711 401 90
BsaBI GATnn'nnATC
Cuts at: 2807 6502 2807
Size: 3695 2813
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4315 4697 5332 275
Size: 53 83 186 936 2782 382 635 1451
Fragments arranged by size:
2782 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3475 3674 4941 5339 5613 5760 6171 3475
Size: 199 1267 398 274 147 411 3812
Fragments arranged by size:
3812 1267 411 398 274 199 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3632 3662 1178
Size: 30 165 30 2229 30 4024
Fragments arranged by size:
4024 2229 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2813 3625 1186
Size: 1627 812 4069
Fragments arranged by size:
4069 1627 812
BseYI C'CCAG_C
Cuts at: 5711 6207 5711
Size: 496 6012
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2127 2657 3382 1163
Size: 573 391 530 725 4289
Fragments arranged by size:
4289 725 573 530 391
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2028 2052 2056 3958 4719 5747
Size: 275 186 27 24 4 1902 761 1028
Cuts at: 5747 1540
Size: 2301
Fragments arranged by size:
2301 1902 1028 761 275 186 27 24
4
BsiHKAI G_wGCw'C
Cuts at: 2316 2414 2937 3249 3377 3674 4569 4654 5725
Size: 98 523 312 128 297 895 85 1071
Cuts at: 5725 2316
Size: 3099
Fragments arranged by size:
3099 1071 895 523 312 297 128 98
85
BsmI GAATG_Cn'
Cuts at: 2391 4019 4112 2391
Size: 1628 93 4787
Fragments arranged by size:
4787 1628 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2316 2414 2598 2646 2937 3175
Size: 399 313 213 98 184 48 291 238
Cuts at: 3175 3249 3377 3473 3674 3827 4569 4654 5725
Size: 74 128 96 201 153 742 85 1071
Cuts at: 5725 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 313 291 238 213
201 184 153 128 98 96 85 74
48
BspEI T'CCGG_A
Cuts at: 6171 6171
Size: 6508
BspHI T'CATG_A
Cuts at: 4397 6127 4397
Size: 1730 4778
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 2629 2698 2829 3863 844
Size: 1400 385 69 131 1034 3489
Fragments arranged by size:
3489 1400 1034 385 131 69
BsrBI CCG'CTC
Cuts at: 1394 1823 2033 2124 2377 4395 1394
Size: 429 210 91 253 2018 3507
Fragments arranged by size:
3507 2018 429 253 210 91
BsrDI GCAATG_nn'
Cuts at: 65 1248 2487 5003 5177 65
Size: 1183 1239 2516 174 1396
Fragments arranged by size:
2516 1396 1239 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 2784 3674 5152 5339 1700
Size: 110 241 733 890 1478 187 2869
Fragments arranged by size:
2869 1478 890 733 241 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6508
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6508
BssSI C'ACGA_G
Cuts at: 4568 5580 4568
Size: 1012 5496
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2494 794
Size: 1700 4808
BstBI TT'CG_AA
Cuts at: 2514 5312 2514
Size: 2798 3710
BstEII G'GTnAC_C
Cuts at: 1184 3257 1184
Size: 2073 4435
BstXI CCAn_nnnn'nTGG
Cuts at: 2204 2204
Size: 6508
BstYI r'GATC_y
Cuts at: 1972 2265 2802 3559 3838 4205 4590 4607 6059
Size: 293 537 757 279 367 385 17 1452
Cuts at: 6059 6145 6157 6503 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 757 537 385 367 346 293
279 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2430 513
Size: 552 1365 4591
Fragments arranged by size:
4591 1365 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2137 487
Size: 683 56 911 4858
Fragments arranged by size:
4858 911 683 56
BtsI GCAGTG_nn'
Cuts at: 3011 3573 4024 4818 4838 3011
Size: 562 451 794 20 4681
Fragments arranged by size:
4681 794 562 451 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6508
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2587 2622 529
Size: 35 2023 35 4415
Fragments arranged by size:
4415 2023 35 35
DraI TTT'AAA
Cuts at: 3812 3812
Size: 6508
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5515 809
Size: 507 4199 1802
Fragments arranged by size:
4199 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2053 3955
Size: 54 1425 50 275 18 195 28 1902
Cuts at: 3955 4844 6473 8
Size: 889 1629 43
Fragments arranged by size:
1902 1629 1425 889 275 195 54 50
43 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 2025 2053 3955 1537
Size: 275 213 28 1902 4090
Fragments arranged by size:
4090 1902 275 213 28
EarI CTCTTCn'nnn_
Cuts at: 1353 4438 1353
Size: 3085 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5095 5469 5615 387
Size: 1064 138 3506 374 146 1280
Fragments arranged by size:
3506 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2412 2935 3247 3375 2412
Size: 523 312 128 5545
Fragments arranged by size:
5545 523 312 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2888 2947 3100 3159 3524 4571
Size: 393 437 819 59 153 59 365 1047
Cuts at: 4571 5168 5955 6223 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1047 819 787 597 437 393 365
268 153 59 59
EcoNI CCTnn'n_nnAGG
Cuts at: 3607 3607
Size: 6508
EcoO109I rG'GnC_Cy
Cuts at: 2674 3469 2674
Size: 795 5713
EcoRI G'AATT_C
Cuts at: 3817 4195 3817
Size: 378 6130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2716 3203 2716
Size: 487 6021
FspI TGC'GCA
Cuts at: 3572 5014 3572
Size: 1442 5066
FspAI rTGC'GCAy
Cuts at: 3572 3572
Size: 6508
HaeII r_GCGC'y
Cuts at: 1678 2073 2578 2779 5655 1678
Size: 395 505 201 2876 2531
Fragments arranged by size:
2876 2531 505 395 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2612 2644 3098 3130 3632
Size: 32 135 32 1040 32 454 32 502
Cuts at: 3632 3664 5149 5181 5223 5255 1373
Size: 32 1485 32 42 32 2626
Fragments arranged by size:
2626 1485 1040 502 454 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 3852 4098 669
Size: 988 281 72 1842 246 3079
Fragments arranged by size:
3079 1842 988 281 246 72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6508
HpaI GTT'AAC
Cuts at: 2010 4098 2010
Size: 2088 4420
KpnI G_GTAC'C
Cuts at: 3833 3833
Size: 6508
MfeI C'AATT_G
Cuts at: 4107 4107
Size: 6508
MluI A'CGCG_T
Cuts at: 6384 6384
Size: 6508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5622 5806 1305
Size: 4317 184 2007
Fragments arranged by size:
4317 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4686
Fragments arranged by size:
4686 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2238 2462 2861 2897 3135 3215 4466
Size: 648 1072 224 399 36 238 80 1251
Cuts at: 4466 4825 4984 518
Size: 359 159 2042
Fragments arranged by size:
2042 1251 1072 648 399 359 238 224
159 80 36
MspA1I CmG'CkG
Cuts at: 1606 2383 2795 3295 3924 4601 5749 5994 1606
Size: 777 412 500 629 677 1148 245 2120
Fragments arranged by size:
2120 1148 777 677 629 500 412 245
NcoI C'CATG_G
Cuts at: 513 1065 2430 513
Size: 552 1365 4591
Fragments arranged by size:
4591 1365 552
NdeI CA'TA_TG
Cuts at: 387 2632 2862 387
Size: 2245 230 4033
Fragments arranged by size:
4033 2245 230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6508
NotI GC'GGCC_GC
Cuts at: 3955 3955
Size: 6508
NsiI A_TGCA'T
Cuts at: 3349 3349
Size: 6508
NspI r_CATG'y
Cuts at: 1132 1229 2243 2497 3208 3325 3866 5411 1132
Size: 97 1014 254 711 117 541 1545 2229
Fragments arranged by size:
2229 1545 1014 711 541 254 117 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6508
PciI A'CATG_T
Cuts at: 5407 5407
Size: 6508
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2746 2866 1108
Size: 1638 120 4750
Fragments arranged by size:
4750 1638 120
PfoI T'CCnGG_A
Cuts at: 2811 3449 2811
Size: 638 5870
PmeI GTTT'AAAC
Cuts at: 3812 3812
Size: 6508
PmlI CAC'GTG
Cuts at: 2656 2746 2656
Size: 90 6418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4577 4609 6090 6122 4577
Size: 32 1481 32 4963
Fragments arranged by size:
4963 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2674 2674
Size: 6508
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6508
PsiI TTA'TAA
Cuts at: 4078 4078
Size: 6508
PspOMI G'GGCC_C
Cuts at: 2594 3469 2594
Size: 875 5633
PstI C_TGCA'G
Cuts at: 830 3006 3568 3860 830
Size: 2176 562 292 3478
Fragments arranged by size:
3478 2176 562 292
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6508
PvuII CAG'CTG
Cuts at: 2383 3295 2383
Size: 912 5596
SacI G_AGCT'C
Cuts at: 2414 2937 3249 3377 2414
Size: 523 312 128 5545
Fragments arranged by size:
5545 523 312 128
SalI G'TCGA_C
Cuts at: 1936 3850 1936
Size: 1914 4594
SbfI CC_TGCA'GG
Cuts at: 3860 3860
Size: 6508
ScaI AGT'ACT
Cuts at: 4756 6468 4756
Size: 1712 4796
SfcI C'TryA_G
Cuts at: 826 1045 3002 3564 3856 4991 5672 5863 826
Size: 219 1957 562 292 1135 681 191 1471
Fragments arranged by size:
1957 1471 1135 681 562 292 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
SgrAI Cr'CCGG_yG
Cuts at: 1700 3674 1700
Size: 1974 4534
SmaI CCC'GGG
Cuts at: 1872 2097 3835 1872
Size: 225 1738 4545
Fragments arranged by size:
4545 1738 225
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2678 3050 4612 5513 5775 820
Size: 197 926 735 372 1562 901 262 1553
Fragments arranged by size:
1562 1553 926 901 735 372 262 197
SnaBI TAC'GTA
Cuts at: 493 4299 493
Size: 3806 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6508
SphI G_CATG'C
Cuts at: 1132 3208 3325 3866 1132
Size: 2076 117 541 3774
Fragments arranged by size:
3774 2076 541 117
SspI AAT'ATT
Cuts at: 5 52 3772 4432 5
Size: 47 3720 660 2081
Fragments arranged by size:
3720 2081 660 47
StuI AGG'CCT
Cuts at: 1517 2720 1517
Size: 1203 5305
StyI C'CwwG_G
Cuts at: 513 1065 2430 3187 3378 3947 513
Size: 552 1365 757 191 569 3074
Fragments arranged by size:
3074 1365 757 569 552 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4535 4552 4705 4890 5335 325
Size: 1255 2955 17 153 185 445 1498
Fragments arranged by size:
2955 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4754 6466 96
Size: 274 80 33 51 1443 2777 1712 138
Fragments arranged by size:
2777 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2619 2651 6468 6500 2619
Size: 32 3817 32 2627
Fragments arranged by size:
3817 2627 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6508
XbaI T'CTAG_A
Cuts at: 3844 3844
Size: 6508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2437 2437
Size: 6508
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6508
XmaI C'CCGG_G
Cuts at: 1870 2095 3833 1870
Size: 225 1738 4545
Fragments arranged by size:
4545 1738 225
XmnI GAAnn'nnTTC
Cuts at: 2777 2951 4637 2777
Size: 174 1686 4648
Fragments arranged by size:
4648 1686 174
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4316 276
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FseI KasI NaeI NarI
NgoMIV NruI PacI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI