Restriction map for
pFN21AE0984
(Circular) (Six-base) MAPSORT of: pFN21AE0984.seq Check: 5430 from: 1 to: 6481
pFN21AE0984 6481 bp
With 182 enzymes: SgfI *
March 28, 2012 13:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4291 278
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3824 1937
Size: 1887 4594
Acc65I G'GTAC_C
Cuts at: 3802 3802
Size: 6481
AclI AA'CG_TT
Cuts at: 1563 4608 4981 1563
Size: 3045 373 3063
Fragments arranged by size:
3063 3045 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3665 4544 5928 6196 1632
Size: 2033 879 1384 268 1917
Fragments arranged by size:
2033 1917 1384 879 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6284
AflIII A'CryG_T
Cuts at: 1203 5380 6357 1203
Size: 4177 977 1327
Fragments arranged by size:
4177 1327 977
AgeI A'CCGG_T
Cuts at: 5312 5312
Size: 6481
AhdI GACnn_n'nnGTC
Cuts at: 5210 5210
Size: 6481
AleI CACnn'nnGTG
Cuts at: 1166 3360 1166
Size: 2194 4287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6481
AlwNI CAG_nnn'CTG
Cuts at: 2462 4224 5796 2462
Size: 1762 1572 3147
Fragments arranged by size:
3147 1762 1572
ApaLI G'TGCA_C
Cuts at: 4538 5694 4538
Size: 1156 5325
ApoI r'AATT_y
Cuts at: 1493 2226 2243 2885 3541 3566 3751 3790 4016
Size: 733 17 642 656 25 185 39 226
Cuts at: 4016 4168 6277 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 733 656 642 226 185 152
39 25 17
AseI AT'TA_AT
Cuts at: 160 1032 2939 5035 160
Size: 872 1907 2096 1606
Fragments arranged by size:
2096 1907 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
AvaI C'yCGr_G
Cuts at: 1870 1943 3806 1870
Size: 73 1863 4545
Fragments arranged by size:
4545 1863 73
BamHI G'GATC_C
Cuts at: 3445 3811 4178 3445
Size: 366 367 5748
Fragments arranged by size:
5748 367 366
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3802 5257 618
Size: 325 217 627 2015 1455 1842
Fragments arranged by size:
2015 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2509 2699 3800 1391
Size: 1118 190 1101 4072
Fragments arranged by size:
4072 1118 1101 190
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5510
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4672 4706 1903
Size: 34 2735 34 3678
Fragments arranged by size:
3678 2735 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2413 4373 5583 2413
Size: 1960 1210 3311
Fragments arranged by size:
3311 1960 1210
BclI T'GATC_A
Cuts at: 1548 2574 1548
Size: 1026 5455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5092 136
Size: 107 122 71 631 605 3420 1525
Fragments arranged by size:
3420 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3551 6476 3551
Size: 2925 3556
BlpI GC'TnA_GC
Cuts at: 3898 3898
Size: 6481
Bme1580I G_kGCm'C
Cuts at: 1790 4542 5698 1790
Size: 2752 1156 2573
Fragments arranged by size:
2752 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6481
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2091 2401 3354
Size: 416 53 83 399 24 674 310 953
Cuts at: 3354 4212 5170 442
Size: 858 958 1753
Fragments arranged by size:
1753 958 953 858 674 416 399 310
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3174 5141 1239
Size: 1935 1967 2579
Fragments arranged by size:
2579 1967 1935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2709 4606 5471 5769 2709
Size: 1897 865 298 3421
Fragments arranged by size:
3421 1897 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2110 5144 882
Size: 617 611 3034 2219
Fragments arranged by size:
3034 2219 617 611
BsaAI yAC'GTr
Cuts at: 493 1204 4272 493
Size: 711 3068 2702
Fragments arranged by size:
3068 2702 711
BsaBI GATnn'nnATC
Cuts at: 3705 6475 3705
Size: 2770 3711
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4288 4670 5305 275
Size: 53 83 186 936 2755 382 635 1451
Fragments arranged by size:
2755 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4914 5312 5586 5733 6144 4914
Size: 398 274 147 411 5251
Fragments arranged by size:
5251 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3270 3300 1178
Size: 30 165 30 1867 30 4359
Fragments arranged by size:
4359 1867 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3120 1186
Size: 1934 4547
BseYI C'CCAG_C
Cuts at: 2708 3236 5684 6180 2708
Size: 528 2448 496 3009
Fragments arranged by size:
3009 2448 528 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5908
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3931 4692 5720 1540
Size: 275 186 1930 761 1028 2301
Fragments arranged by size:
2301 1930 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2509 4542 4627 5698 2509
Size: 2033 85 1071 3292
Fragments arranged by size:
3292 2033 1071 85
BsmI GAATG_Cn'
Cuts at: 3992 4085 3992
Size: 93 6388
BsmBI CGTCTCn'nnnn_
Cuts at: 3385 3385
Size: 6481
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2509 2699 3800 4542 4627 5698 1391
Size: 399 719 190 1101 742 85 1071 2174
Fragments arranged by size:
2174 1101 1071 742 719 399 190 85
BspEI T'CCGG_A
Cuts at: 6144 6144
Size: 6481
BspHI T'CATG_A
Cuts at: 2058 2851 4370 6100 2058
Size: 793 1519 1730 2439
Fragments arranged by size:
2439 1730 1519 793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3836 844
Size: 2992 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4368 1394
Size: 429 2545 3507
Fragments arranged by size:
3507 2545 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3271 3736 4976 5150 65
Size: 1183 2023 465 1240 174 1396
Fragments arranged by size:
2023 1396 1240 1183 465 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5125 5312 1700
Size: 110 3315 187 2869
Fragments arranged by size:
3315 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6481
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6481
BssSI C'ACGA_G
Cuts at: 4541 5553 4541
Size: 1012 5469
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6481
BstBI TT'CG_AA
Cuts at: 5285 5285
Size: 6481
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6481
BstYI r'GATC_y
Cuts at: 1972 2328 3445 3551 3811 4178 4563 4580 6032
Size: 356 1117 106 260 367 385 17 1452
Cuts at: 6032 6118 6130 6476 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1117 385 367 356 346 260
106 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4990
Fragments arranged by size:
4990 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2159 487
Size: 683 56 933 4809
Fragments arranged by size:
4809 933 683 56
BtsI GCAGTG_nn'
Cuts at: 3011 3997 4791 4811 3011
Size: 986 794 20 4681
Fragments arranged by size:
4681 986 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6481
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6446
DraI TTT'AAA
Cuts at: 3785 3785
Size: 6481
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5488 809
Size: 507 4172 1802
Fragments arranged by size:
4172 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2487 2586
Size: 54 1425 50 275 18 264 393 99
Cuts at: 2586 3733 3928 4817 6446 8
Size: 1147 195 889 1629 43
Fragments arranged by size:
1629 1425 1147 889 393 275 264 195
99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3928 1537
Size: 275 2116 4090
Fragments arranged by size:
4090 2116 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2327 3097 3641 3752 4411 1353
Size: 974 770 544 111 659 3423
Fragments arranged by size:
3423 974 770 659 544 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5068 5442 5588 387
Size: 1064 138 3479 374 146 1280
Fragments arranged by size:
3479 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2507 2507
Size: 6481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3174 3665 4544 5141 5928 6196 1239
Size: 393 1542 491 879 597 787 268 1524
Fragments arranged by size:
1542 1524 879 787 597 491 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3452 3452
Size: 6481
EcoRI G'AATT_C
Cuts at: 3566 3790 4168 3566
Size: 224 378 5879
Fragments arranged by size:
5879 378 224
EcoRV GAT'ATC
Cuts at: 2554 2829 3112 2554
Size: 275 283 5923
Fragments arranged by size:
5923 283 275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3164 3164
Size: 6481
FspI TGC'GCA
Cuts at: 4987 4987
Size: 6481
HaeII r_GCGC'y
Cuts at: 1678 5628 1678
Size: 3950 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2813 2845 3096 3128 5122
Size: 32 135 32 1241 32 251 32 1994
Cuts at: 5122 5154 5196 5228 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1994 1241 251 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2134 3155 3372 3825 4071 669
Size: 988 281 196 1021 217 453 246 3079
Fragments arranged by size:
3079 1021 988 453 281 246 217 196
HindIII A'AGCT_T
Cuts at: 748 2765 748
Size: 2017 4464
HpaI GTT'AAC
Cuts at: 3372 4071 3372
Size: 699 5782
KpnI G_GTAC'C
Cuts at: 3806 3806
Size: 6481
MfeI C'AATT_G
Cuts at: 2340 4080 2340
Size: 1740 4741
MluI A'CGCG_T
Cuts at: 6357 6357
Size: 6481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5595 5779 1305
Size: 4290 184 2007
Fragments arranged by size:
4290 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 2489 2588 3735 10
Size: 54 1425 343 264 393 99 1147 2756
Fragments arranged by size:
2756 1425 1147 393 343 264 99 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2582 3360 4439 4798 4957 518
Size: 648 843 573 778 1079 359 159 2042
Fragments arranged by size:
2042 1079 843 778 648 573 359 159
MspA1I CmG'CkG
Cuts at: 1606 3897 4574 5722 5967 1606
Size: 2291 677 1148 245 2120
Fragments arranged by size:
2291 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4990
Fragments arranged by size:
4990 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6481
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6481
NotI GC'GGCC_GC
Cuts at: 3928 3928
Size: 6481
NspI r_CATG'y
Cuts at: 1132 1229 3839 5384 1132
Size: 97 2610 1545 2229
Fragments arranged by size:
2610 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6481
PciI A'CATG_T
Cuts at: 5380 5380
Size: 6481
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2496 1108
Size: 1388 5093
PmeI GTTT'AAAC
Cuts at: 3785 3785
Size: 6481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4550 4582 6063 6095 4550
Size: 32 1481 32 4936
Fragments arranged by size:
4936 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3452 3452
Size: 6481
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6481
PsiI TTA'TAA
Cuts at: 4051 4051
Size: 6481
PstI C_TGCA'G
Cuts at: 830 2919 3833 830
Size: 2089 914 3478
Fragments arranged by size:
3478 2089 914
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6481
SacI G_AGCT'C
Cuts at: 2509 2509
Size: 6481
SalI G'TCGA_C
Cuts at: 1936 3823 1936
Size: 1887 4594
SbfI CC_TGCA'GG
Cuts at: 3833 3833
Size: 6481
ScaI AGT'ACT
Cuts at: 4729 6441 4729
Size: 1712 4769
SexAI A'CCwGG_T
Cuts at: 2187 2187
Size: 6481
SfcI C'TryA_G
Cuts at: 826 1045 2915 3829 4964 5645 5836 826
Size: 219 1870 914 1135 681 191 1471
Fragments arranged by size:
1870 1471 1135 914 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6481
SmaI CCC'GGG
Cuts at: 1872 3808 1872
Size: 1936 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2724 4585 5486 5748 820
Size: 197 926 781 1861 901 262 1553
Fragments arranged by size:
1861 1553 926 901 781 262 197
SnaBI TAC'GTA
Cuts at: 493 4272 493
Size: 3779 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6481
SphI G_CATG'C
Cuts at: 1132 3839 1132
Size: 2707 3774
SspI AAT'ATT
Cuts at: 5 52 2395 4405 5
Size: 47 2343 2010 2081
Fragments arranged by size:
2343 2081 2010 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6481
StyI C'CwwG_G
Cuts at: 513 1065 2004 2023 3063 3920 513
Size: 552 939 19 1040 857 3074
Fragments arranged by size:
3074 1040 939 857 552 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3313 4508 4525 4678 4863 5308 325
Size: 1255 1733 1195 17 153 185 445 1498
Fragments arranged by size:
1733 1498 1255 1195 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4727 6439 96
Size: 274 80 33 51 1443 2750 1712 138
Fragments arranged by size:
2750 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2532 2564 2581 2613 3270 3302 6441 6473 2532
Size: 32 17 32 657 32 3139 32 2540
Fragments arranged by size:
3139 2540 657 32 32 32 32 17
Tth111I GACn'n_nGTC
Cuts at: 1316 3150 1316
Size: 1834 4647
XbaI T'CTAG_A
Cuts at: 3817 3817
Size: 6481
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6481
XmaI C'CCGG_G
Cuts at: 1870 3806 1870
Size: 1936 4545
XmnI GAAnn'nnTTC
Cuts at: 3174 3566 4610 3174
Size: 392 1044 5045
Fragments arranged by size:
5045 1044 392
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4289 276
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI