Restriction map for
pFN21AE0982
(Circular) (Six-base) MAPSORT of: pFN21AE0982.seq Check: 3122 from: 1 to: 6466
pFN21AE0982 6466 bp
With 182 enzymes: SgfI *
March 28, 2012 12:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3175 3175
Size: 6466
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4276 278
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3809 1937
Size: 1872 4594
Acc65I G'GTAC_C
Cuts at: 2583 3072 3787 2583
Size: 489 715 5262
Fragments arranged by size:
5262 715 489
AclI AA'CG_TT
Cuts at: 1563 4593 4966 1563
Size: 3030 373 3063
Fragments arranged by size:
3063 3030 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2474 2862 3665 4529 5913 6181 1632
Size: 842 388 803 864 1384 268 1917
Fragments arranged by size:
1917 1384 864 842 803 388 268
AfeI AGC'GCT
Cuts at: 3224 3224
Size: 6466
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6269
AflIII A'CryG_T
Cuts at: 1203 2637 3057 3624 5365 6342 1203
Size: 1434 420 567 1741 977 1327
Fragments arranged by size:
1741 1434 1327 977 567 420
AgeI A'CCGG_T
Cuts at: 3114 5297 3114
Size: 2183 4283
AhdI GACnn_n'nnGTC
Cuts at: 5195 5195
Size: 6466
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6466
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2291 1184
Size: 1107 5359
AlwNI CAG_nnn'CTG
Cuts at: 2805 4209 5781 2805
Size: 1404 1572 3490
Fragments arranged by size:
3490 1572 1404
ApaI G_GGCC'C
Cuts at: 2701 2701
Size: 6466
ApaLI G'TGCA_C
Cuts at: 4523 5679 4523
Size: 1156 5310
ApoI r'AATT_y
Cuts at: 1493 3775 4001 4153 6262 1493
Size: 2282 226 152 2109 1697
Fragments arranged by size:
2282 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3408 3408
Size: 6466
AseI AT'TA_AT
Cuts at: 160 1032 5020 160
Size: 872 3988 1606
Fragments arranged by size:
3988 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
AvaI C'yCGr_G
Cuts at: 1870 1943 3545 3662 3713 3791 1870
Size: 73 1602 117 51 78 4545
Fragments arranged by size:
4545 1602 117 78 73 51
BamHI G'GATC_C
Cuts at: 3796 4163 3796
Size: 367 6099
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2214 2583 3072 3117 3163
Size: 325 217 627 427 369 489 45 46
Cuts at: 3163 3599 3787 5242 618
Size: 436 188 1455 1842
Fragments arranged by size:
1842 1455 627 489 436 427 369 325
217 188 46 45
BanII G_rGCy'C
Cuts at: 1391 2415 2701 2945 2970 3366 3785 1391
Size: 1024 286 244 25 396 419 4072
Fragments arranged by size:
4072 1024 419 396 286 244 25
BbeI G_GCGC'C
Cuts at: 2218 3167 3603 2218
Size: 949 436 5081
Fragments arranged by size:
5081 949 436
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2763 928
Size: 971 864 4631
Fragments arranged by size:
4631 971 864
BbvCI CC'TCA_GC
Cuts at: 3174 3174
Size: 6466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4657 4691 1903
Size: 34 2720 34 3678
Fragments arranged by size:
3678 2720 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4358 5568 4358
Size: 1210 5256
BclI T'GATC_A
Cuts at: 1548 2530 1548
Size: 982 5484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3266 5077 136
Size: 107 122 71 631 605 1594 1811 1525
Fragments arranged by size:
1811 1594 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6461 6461
Size: 6466
BlpI GC'TnA_GC
Cuts at: 3883 3883
Size: 6466
Bme1580I G_kGCm'C
Cuts at: 1790 2270 2363 2701 2957 4527 5683 1790
Size: 480 93 338 256 1570 1156 2573
Fragments arranged by size:
2573 1570 1156 480 338 256 93
BmgBI CAC'GTC
Cuts at: 1318 3181 3625 1318
Size: 1863 444 4159
Fragments arranged by size:
4159 1863 444
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3061 3604 3695
Size: 416 53 83 399 24 1644 543 91
Cuts at: 3695 4197 5155 442
Size: 502 958 1753
Fragments arranged by size:
1753 1644 958 543 502 416 399 91
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6466
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2989 3766 2989
Size: 777 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 2729 2750 2891 3119 3497 5126 1239
Size: 1133 357 21 141 228 378 1629 2579
Fragments arranged by size:
2579 1629 1133 378 357 228 141 21
Bpu10I CC'TnA_GC
Cuts at: 2162 2774 3174 2162
Size: 612 400 5454
Fragments arranged by size:
5454 612 400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2399 2480 4591 5456 5754 2399
Size: 81 2111 865 298 3111
Fragments arranged by size:
3111 2111 865 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2324 2726 5129 882
Size: 617 825 402 2403 2219
Fragments arranged by size:
2403 2219 825 617 402
BsaAI yAC'GTr
Cuts at: 493 1204 3060 4257 493
Size: 711 1856 1197 2702
Fragments arranged by size:
2702 1856 1197 711
BsaBI GATnn'nnATC
Cuts at: 2204 6460 2204
Size: 4256 2210
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2215 3126 3164 3600
Size: 53 83 186 936 682 911 38 436
Cuts at: 3600 4273 4655 5290 275
Size: 673 382 635 1451
Fragments arranged by size:
1451 936 911 682 673 635 436 382
186 83 53 38
BsaWI w'CCGG_w
Cuts at: 3114 4899 5297 5571 5718 6129 3114
Size: 1785 398 274 147 411 3451
Fragments arranged by size:
3451 1785 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2411 2441 2586 2616 3200
Size: 30 165 30 1008 30 145 30 584
Cuts at: 3200 3230 3266 3296 1178
Size: 30 36 30 4348
Fragments arranged by size:
4348 1008 584 165 145 36 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6466
BseYI C'CCAG_C
Cuts at: 2693 5669 6165 2693
Size: 2976 496 2994
Fragments arranged by size:
2994 2976 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2426 3082 3203 3637 3755 1163
Size: 573 690 656 121 434 118 3874
Fragments arranged by size:
3874 690 656 573 434 121 118
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2115 3405 3577 3916 4677 5705
Size: 275 186 114 1290 172 339 761 1028
Cuts at: 5705 1540
Size: 2301
Fragments arranged by size:
2301 1290 1028 761 339 275 186 172
114
BsiHKAI G_wGCw'C
Cuts at: 2256 2496 3503 3522 3720 4527 4612 5683 2256
Size: 240 1007 19 198 807 85 1071 3039
Fragments arranged by size:
3039 1071 1007 807 240 198 85 19
BsmI GAATG_Cn'
Cuts at: 2688 3977 4070 2688
Size: 1289 93 5084
Fragments arranged by size:
5084 1289 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2256 2270 2363 2415 2496 2701 2945
Size: 399 466 14 93 52 81 205 244
Cuts at: 2945 2957 2970 3366 3503 3522 3720 3785 4527
Size: 12 13 396 137 19 198 65 742
Cuts at: 4527 4612 5683 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 466 399 396 244 205
198 137 93 85 81 65 52 19
14 13 12
BspEI T'CCGG_A
Cuts at: 6129 6129
Size: 6466
BspHI T'CATG_A
Cuts at: 4355 6085 4355
Size: 1730 4736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2283 3175 3678 3821 844
Size: 1439 892 503 143 3489
Fragments arranged by size:
3489 1439 892 503 143
BsrBI CCG'CTC
Cuts at: 1394 1823 4353 1394
Size: 429 2530 3507
Fragments arranged by size:
3507 2530 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4961 5135 65
Size: 1183 3713 174 1396
Fragments arranged by size:
3713 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2648 3114 3404 3460 5110 5297 1700
Size: 110 838 466 290 56 1650 187 2869
Fragments arranged by size:
2869 1650 838 466 290 187 110 56
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6466
BssHII G'CGCG_C
Cuts at: 1925 3151 3256 3408 3446 1925
Size: 1226 105 152 38 4945
Fragments arranged by size:
4945 1226 152 105 38
BssSI C'ACGA_G
Cuts at: 2489 2950 3731 4526 5538 2489
Size: 461 781 795 1012 3417
Fragments arranged by size:
3417 1012 795 781 461
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6466
BstBI TT'CG_AA
Cuts at: 5270 5270
Size: 6466
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6466
BstXI CCAn_nnnn'nTGG
Cuts at: 3430 3430
Size: 6466
BstYI r'GATC_y
Cuts at: 1972 2877 3796 4163 4548 4565 6017 6103 6115
Size: 905 919 367 385 17 1452 86 12
Cuts at: 6115 6461 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 919 905 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2958 2958
Size: 6466
BtgI C'CryG_G
Cuts at: 513 1065 2004 2115 2407 2895 3249 3417 3423
Size: 552 939 111 292 488 354 168 6
Cuts at: 3423 513
Size: 3556
Fragments arranged by size:
3556 939 552 488 354 292 168 111
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2011 2181 487
Size: 683 56 785 170 4772
Fragments arranged by size:
4772 785 683 170 56
BtsI GCAGTG_nn'
Cuts at: 3494 3982 4776 4796 3494
Size: 488 794 20 5164
Fragments arranged by size:
5164 794 488 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6466
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6431
DraI TTT'AAA
Cuts at: 3770 3770
Size: 6466
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2234 5473 809
Size: 507 918 3239 1802
Fragments arranged by size:
3239 1802 918 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 3024 3402
Size: 54 1425 50 275 18 282 912 378
Cuts at: 3402 3420 3462 3574 3913 4802 6431 8
Size: 18 42 112 339 889 1629 43
Fragments arranged by size:
1629 1425 912 889 378 339 282 275
112 54 50 43 42 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2112 3402 3574 3913 1537
Size: 275 300 1290 172 339 4090
Fragments arranged by size:
4090 1290 339 300 275 172
EarI CTCTTCn'nnn_
Cuts at: 1353 2367 2810 3476 4396 1353
Size: 1014 443 666 920 3423
Fragments arranged by size:
3423 1014 920 666 443
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2419 3449 5053 5427 5573 387
Size: 1064 138 830 1030 1604 374 146 1280
Fragments arranged by size:
1604 1280 1064 1030 830 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 2474 2729 2750 2862 2891 3119
Size: 393 740 102 255 21 112 29 228
Cuts at: 3119 3497 3665 4529 5126 5913 6181 1239
Size: 378 168 864 597 787 268 1524
Fragments arranged by size:
1524 864 787 740 597 393 378 268
255 228 168 112 102 29 21
EcoO109I rG'GnC_Cy
Cuts at: 2449 2485 2697 3667 2449
Size: 36 212 970 5248
Fragments arranged by size:
5248 970 212 36
EcoRI G'AATT_C
Cuts at: 3775 4153 3775
Size: 378 6088
FspI TGC'GCA
Cuts at: 2828 4972 2828
Size: 2144 4322
HaeII r_GCGC'y
Cuts at: 1678 2062 2218 3167 3226 3283 3553 3603 5613
Size: 384 156 949 59 57 270 50 2010
Cuts at: 5613 1678
Size: 2531
Fragments arranged by size:
2531 2010 949 384 270 156 59 57
50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2515 2547 5107 5139 5181
Size: 32 135 32 943 32 2560 32 42
Cuts at: 5181 5213 1373
Size: 32 2626
Fragments arranged by size:
2626 2560 943 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2068 3810 4056 669
Size: 988 281 130 1742 246 3079
Fragments arranged by size:
3079 1742 988 281 246 130
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6466
HpaI GTT'AAC
Cuts at: 4056 4056
Size: 6466
KasI G'GCGC_C
Cuts at: 2214 3163 3599 2214
Size: 949 436 5081
Fragments arranged by size:
5081 949 436
KpnI G_GTAC'C
Cuts at: 2587 3076 3791 2587
Size: 489 715 5262
Fragments arranged by size:
5262 715 489
MfeI C'AATT_G
Cuts at: 4065 4065
Size: 6466
MluI A'CGCG_T
Cuts at: 6342 6342
Size: 6466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2098 3072 5580 5764 1305
Size: 793 974 2508 184 2007
Fragments arranged by size:
2508 2007 974 793 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4644
Fragments arranged by size:
4644 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2636 2950 4424 4783 4942 518
Size: 648 1470 314 1474 359 159 2042
Fragments arranged by size:
2042 1474 1470 648 359 314 159
MspA1I CmG'CkG
Cuts at: 1606 2401 2409 2912 3043 3882 4559 5707 5952
Size: 795 8 503 131 839 677 1148 245
Cuts at: 5952 1606
Size: 2120
Fragments arranged by size:
2120 1148 839 795 677 503 245 131
8
NaeI GCC'GGC
Cuts at: 2650 3406 3462 2650
Size: 756 56 5654
Fragments arranged by size:
5654 756 56
NarI GG'CG_CC
Cuts at: 2215 3164 3600 2215
Size: 949 436 5081
Fragments arranged by size:
5081 949 436
NcoI C'CATG_G
Cuts at: 513 1065 2004 2895 3249 513
Size: 552 939 891 354 3730
Fragments arranged by size:
3730 939 891 552 354
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6466
NgoMIV G'CCGG_C
Cuts at: 2648 3404 3460 2648
Size: 756 56 5654
Fragments arranged by size:
5654 756 56
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6466
NotI GC'GGCC_GC
Cuts at: 3913 3913
Size: 6466
NspI r_CATG'y
Cuts at: 1132 1229 2381 2641 3824 5369 1132
Size: 97 1152 260 1183 1545 2229
Fragments arranged by size:
2229 1545 1183 1152 260 97
PasI CC'CwG_GG
Cuts at: 1348 2080 1348
Size: 732 5734
PciI A'CATG_T
Cuts at: 2637 5365 2637
Size: 2728 3738
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2080 2707 1108
Size: 972 627 4867
Fragments arranged by size:
4867 972 627
PfoI T'CCnGG_A
Cuts at: 2316 2727 2748 3375 3474 2316
Size: 411 21 627 99 5308
Fragments arranged by size:
5308 627 411 99 21
PmeI GTTT'AAAC
Cuts at: 3770 3770
Size: 6466
PmlI CAC'GTG
Cuts at: 3060 3060
Size: 6466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4535 4567 6048 6080 4535
Size: 32 1481 32 4921
Fragments arranged by size:
4921 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2449 2485 3667 2449
Size: 36 1182 5248
Fragments arranged by size:
5248 1182 36
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6466
PsiI TTA'TAA
Cuts at: 4036 4036
Size: 6466
PspOMI G'GGCC_C
Cuts at: 2697 2697
Size: 6466
PstI C_TGCA'G
Cuts at: 830 2280 3818 830
Size: 1450 1538 3478
Fragments arranged by size:
3478 1538 1450
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6466
PvuII CAG'CTG
Cuts at: 2401 2912 3043 2401
Size: 511 131 5824
Fragments arranged by size:
5824 511 131
SacII CC_GC'GG
Cuts at: 2410 2410
Size: 6466
SalI G'TCGA_C
Cuts at: 1936 3808 1936
Size: 1872 4594
SapI GCTCTTCn'nnn_
Cuts at: 2367 2367
Size: 6466
SbfI CC_TGCA'GG
Cuts at: 2280 3818 2280
Size: 1538 4928
ScaI AGT'ACT
Cuts at: 4714 6426 4714
Size: 1712 4754
SfcI C'TryA_G
Cuts at: 826 1045 2276 3814 4949 5630 5821 826
Size: 219 1231 1538 1135 681 191 1471
Fragments arranged by size:
1538 1471 1231 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2216 3165 3601 2216
Size: 949 436 5081
Fragments arranged by size:
5081 949 436
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6466
SmaI CCC'GGG
Cuts at: 1872 3793 1872
Size: 1921 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2414 2495 4570 5471 5733 820
Size: 197 926 471 81 2075 901 262 1553
Fragments arranged by size:
2075 1553 926 901 471 262 197 81
SnaBI TAC'GTA
Cuts at: 493 4257 493
Size: 3764 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6466
SphI G_CATG'C
Cuts at: 1132 2381 3824 1132
Size: 1249 1443 3774
Fragments arranged by size:
3774 1443 1249
SspI AAT'ATT
Cuts at: 5 52 4390 5
Size: 47 4338 2081
Fragments arranged by size:
4338 2081 47
StuI AGG'CCT
Cuts at: 1517 2821 3746 1517
Size: 1304 925 4237
Fragments arranged by size:
4237 1304 925
StyI C'CwwG_G
Cuts at: 513 1065 2004 2262 2895 3249 3905 513
Size: 552 939 258 633 354 656 3074
Fragments arranged by size:
3074 939 656 633 552 354 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2246 2348 3755 4493 4510 4663 4848
Size: 1255 666 102 1407 738 17 153 185
Cuts at: 4848 5293 325
Size: 445 1498
Fragments arranged by size:
1498 1407 1255 738 666 445 185 153
102 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 2418 4712
Size: 274 80 33 51 1443 345 96 2294
Cuts at: 4712 6424 96
Size: 1712 138
Fragments arranged by size:
2294 1712 1443 345 274 138 96 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2586 2618 3086 3118 3200 3232 6426 6458 2586
Size: 32 468 32 82 32 3194 32 2594
Fragments arranged by size:
3194 2594 468 82 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6466
XbaI T'CTAG_A
Cuts at: 3802 3802
Size: 6466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2866 3373 3472 2866
Size: 507 99 5860
Fragments arranged by size:
5860 507 99
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6466
XmaI C'CCGG_G
Cuts at: 1870 3791 1870
Size: 1921 4545
XmnI GAAnn'nnTTC
Cuts at: 4595 4595
Size: 6466
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4274 276
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PspXI PsrI RsrII SacI SanDI SexAI SfiI
SrfI SwaI