Restriction map for
pFN21AE0979
(Circular) (Six-base) MAPSORT of: pFN21AE0979.seq Check: 6990 from: 1 to: 6427
pFN21AE0979 6427 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2108 3194 2108
Size: 1086 5341
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4237 278
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2589 3770 1937
Size: 652 1181 4594
Fragments arranged by size:
4594 1181 652
Acc65I G'GTAC_C
Cuts at: 3616 3748 3616
Size: 132 6295
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2097 2274 2303 2673 4490 5874 6142 1632
Size: 465 177 29 370 1817 1384 268 1917
Fragments arranged by size:
1917 1817 1384 465 370 268 177 29
AfeI AGC'GCT
Cuts at: 2855 2855
Size: 6427
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6230
AflIII A'CryG_T
Cuts at: 1203 3192 5326 6303 1203
Size: 1989 2134 977 1327
Fragments arranged by size:
2134 1989 1327 977
AgeI A'CCGG_T
Cuts at: 2064 5258 2064
Size: 3194 3233
AhdI GACnn_n'nnGTC
Cuts at: 3196 5156 3196
Size: 1960 4467
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6427
AlwNI CAG_nnn'CTG
Cuts at: 2336 4170 5742 2336
Size: 1834 1572 3021
Fragments arranged by size:
3021 1834 1572
ApaLI G'TGCA_C
Cuts at: 4484 5640 4484
Size: 1156 5271
ApoI r'AATT_y
Cuts at: 1493 2104 2443 2639 2796 2875 3535 3736 3962
Size: 611 339 196 157 79 660 201 226
Cuts at: 3962 4114 6223 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 660 611 339 226 201 196
157 152 79
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 3752 1870
Size: 73 1809 4545
Fragments arranged by size:
4545 1809 73
AvrII C'CTAG_G
Cuts at: 3119 3119
Size: 6427
BamHI G'GATC_C
Cuts at: 3299 3757 4124 3299
Size: 458 367 5602
Fragments arranged by size:
5602 458 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2565 3616 3748 5203 618
Size: 325 217 627 778 1051 132 1455 1842
Fragments arranged by size:
1842 1455 1051 778 627 325 217 132
BanII G_rGCy'C
Cuts at: 1391 2912 3746 1391
Size: 1521 834 4072
Fragments arranged by size:
4072 1521 834
BbeI G_GCGC'C
Cuts at: 2569 2569
Size: 6427
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3485 928
Size: 971 1586 3870
Fragments arranged by size:
3870 1586 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4618 4652 1903
Size: 34 2681 34 3678
Fragments arranged by size:
3678 2681 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4319 5529 4319
Size: 1210 5217
BclI T'GATC_A
Cuts at: 1548 2386 1548
Size: 838 5589
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3122 5038
Size: 107 122 71 631 605 337 1113 1916
Cuts at: 5038 136
Size: 1525
Fragments arranged by size:
1916 1525 1113 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 3655 6422 3655
Size: 2767 3660
BlpI GC'TnA_GC
Cuts at: 2658 3844 2658
Size: 1186 5241
Bme1580I G_kGCm'C
Cuts at: 1790 3170 4488 5644 1790
Size: 1380 1318 1156 2573
Fragments arranged by size:
2573 1380 1318 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6427
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2720 4158 5116
Size: 416 53 83 399 24 1303 1438 958
Cuts at: 5116 442
Size: 1753
Fragments arranged by size:
1753 1438 1303 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3048 1056
Size: 1992 4435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2435 3501 5087 1239
Size: 1196 1066 1586 2579
Fragments arranged by size:
2579 1586 1196 1066
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3397 4552 5417 5715 3397
Size: 1155 865 298 4109
Fragments arranged by size:
4109 1155 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5090 882
Size: 617 3591 2219
Fragments arranged by size:
3591 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4218 493
Size: 711 3014 2702
Fragments arranged by size:
3014 2702 711
BsaBI GATnn'nnATC
Cuts at: 3660 6421 3660
Size: 2761 3666
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2566 3103 4234
Size: 53 83 186 936 479 554 537 1131
Cuts at: 4234 4616 5251 275
Size: 382 635 1451
Fragments arranged by size:
1451 1131 936 635 554 537 479 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2064 4860 5258 5532 5679 6090 2064
Size: 2796 398 274 147 411 2401
Fragments arranged by size:
2796 2401 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2408 2438 1178
Size: 30 165 30 1005 30 5167
Fragments arranged by size:
5167 1005 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6427
BseYI C'CCAG_C
Cuts at: 2055 2336 2543 3040 5630 6126 2055
Size: 281 207 497 2590 496 2356
Fragments arranged by size:
2590 2356 497 496 281 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5854
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3877 4638 5666 1540
Size: 275 186 1876 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3148 4488 4573 5644 3148
Size: 1340 85 1071 3931
Fragments arranged by size:
3931 1340 1071 85
BsmI GAATG_Cn'
Cuts at: 2505 3938 4031 2505
Size: 1433 93 4901
Fragments arranged by size:
4901 1433 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2912 3148 3170 3746 4488 4573 5644
Size: 399 1122 236 22 576 742 85 1071
Cuts at: 5644 1391
Size: 2174
Fragments arranged by size:
2174 1122 1071 742 576 399 236 85
22
BspEI T'CCGG_A
Cuts at: 6090 6090
Size: 6427
BspHI T'CATG_A
Cuts at: 2925 4316 6046 2925
Size: 1391 1730 3306
Fragments arranged by size:
3306 1730 1391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2108 3100 3116 3194 3645 3782 844
Size: 1264 992 16 78 451 137 3489
Fragments arranged by size:
3489 1264 992 451 137 78 16
BsrBI CCG'CTC
Cuts at: 1394 1823 4314 1394
Size: 429 2491 3507
Fragments arranged by size:
3507 2491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4922 5096 65
Size: 1183 3674 174 1396
Fragments arranged by size:
3674 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2064 5071 5258 1700
Size: 110 254 3007 187 2869
Fragments arranged by size:
3007 2869 254 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6427
BssSI C'ACGA_G
Cuts at: 4487 5499 4487
Size: 1012 5415
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6427
BstBI TT'CG_AA
Cuts at: 5231 5231
Size: 6427
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6427
BstYI r'GATC_y
Cuts at: 1972 2831 3299 3655 3757 4124 4509 4526 5978
Size: 859 468 356 102 367 385 17 1452
Cuts at: 5978 6064 6076 6422 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 859 468 385 367 356 346
102 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2733 2733
Size: 6427
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4936
Fragments arranged by size:
4936 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5688
Fragments arranged by size:
5688 683 56
BtsI GCAGTG_nn'
Cuts at: 2922 3943 4737 4757 2922
Size: 1021 794 20 4592
Fragments arranged by size:
4592 1021 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2925 2960 529
Size: 35 2361 35 3996
Fragments arranged by size:
3996 2361 35 35
DraI TTT'AAA
Cuts at: 2088 2110 3544 3712 3731 2088
Size: 22 1434 168 19 4784
Fragments arranged by size:
4784 1434 168 22 19
DraIII CAC_nnn'GTG
Cuts at: 2720 2720
Size: 6427
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5434 809
Size: 507 4118 1802
Fragments arranged by size:
4118 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 3874 4763
Size: 54 1425 50 275 18 396 1648 889
Cuts at: 4763 6392 8
Size: 1629 43
Fragments arranged by size:
1648 1629 1425 889 396 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3874 1537
Size: 275 2062 4090
Fragments arranged by size:
4090 2062 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 4357 1353
Size: 897 2107 3423
Fragments arranged by size:
3423 2107 897
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5014 5388 5534 387
Size: 1064 138 3425 374 146 1280
Fragments arranged by size:
3425 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2274 2303 2435 2673 3501 4490
Size: 393 465 177 29 132 238 828 989
Cuts at: 4490 5087 5874 6142 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 989 828 787 597 465 393 268
238 177 132 29
EcoNI CCTnn'n_nnAGG
Cuts at: 3123 3123
Size: 6427
EcoO109I rG'GnC_Cy
Cuts at: 2249 2249
Size: 6427
EcoRI G'AATT_C
Cuts at: 2443 3535 3736 4114 2443
Size: 1092 201 378 4756
Fragments arranged by size:
4756 1092 378 201
EcoRV GAT'ATC
Cuts at: 3239 3239
Size: 6427
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 6427
FspI TGC'GCA
Cuts at: 4933 4933
Size: 6427
HaeII r_GCGC'y
Cuts at: 1678 2569 2857 5574 1678
Size: 891 288 2717 2531
Fragments arranged by size:
2717 2531 891 288
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2408 2440 2735 2767 2939
Size: 32 135 32 836 32 295 32 172
Cuts at: 2939 2971 3182 3214 3297 3329 5068 5100 5142
Size: 32 211 32 83 32 1739 32 42
Cuts at: 5142 5174 1373
Size: 32 2626
Fragments arranged by size:
2626 1739 836 295 211 172 135 83
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3429 3771 4017 669
Size: 988 281 1491 342 246 3079
Fragments arranged by size:
3079 1491 988 342 281 246
HindIII A'AGCT_T
Cuts at: 748 2141 2493 2763 3492 3627 3690 748
Size: 1393 352 270 729 135 63 3485
Fragments arranged by size:
3485 1393 729 352 270 135 63
HpaI GTT'AAC
Cuts at: 3429 4017 3429
Size: 588 5839
KasI G'GCGC_C
Cuts at: 2565 2565
Size: 6427
KpnI G_GTAC'C
Cuts at: 3620 3752 3620
Size: 132 6295
MfeI C'AATT_G
Cuts at: 3186 4026 3186
Size: 840 5587
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2309 5541 5725 1305
Size: 1004 3232 184 2007
Fragments arranged by size:
3232 2007 1004 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2228 10
Size: 54 1425 343 396 4209
Fragments arranged by size:
4209 1425 396 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2532 4385 4744 4903 518
Size: 648 1366 1853 359 159 2042
Fragments arranged by size:
2042 1853 1366 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2312 3843 4520 5668 5913 1606
Size: 706 1531 677 1148 245 2120
Fragments arranged by size:
2120 1531 1148 706 677 245
NarI GG'CG_CC
Cuts at: 2566 2566
Size: 6427
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4936
Fragments arranged by size:
4936 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6427
NheI G'CTAG_C
Cuts at: 1052 3044 1052
Size: 1992 4435
NotI GC'GGCC_GC
Cuts at: 3874 3874
Size: 6427
NspI r_CATG'y
Cuts at: 1132 1229 3196 3452 3785 5330 1132
Size: 97 1967 256 333 1545 2229
Fragments arranged by size:
2229 1967 1545 333 256 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6427
PciI A'CATG_T
Cuts at: 3192 5326 3192
Size: 2134 4293
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2720 1108
Size: 1612 4815
PfoI T'CCnGG_A
Cuts at: 3517 3517
Size: 6427
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 6009 6041 4496
Size: 32 1481 32 4882
Fragments arranged by size:
4882 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2249 2249
Size: 6427
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6427
PsiI TTA'TAA
Cuts at: 3249 3997 3249
Size: 748 5679
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2602 2634 2602
Size: 32 6395
PstI C_TGCA'G
Cuts at: 830 2917 3205 3779 830
Size: 2087 288 574 3478
Fragments arranged by size:
3478 2087 574 288
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
PvuII CAG'CTG
Cuts at: 2312 2312
Size: 6427
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SbfI CC_TGCA'GG
Cuts at: 3779 3779
Size: 6427
ScaI AGT'ACT
Cuts at: 4675 6387 4675
Size: 1712 4715
SfcI C'TryA_G
Cuts at: 826 1045 2835 2913 3180 3201 3775 4910 5591
Size: 219 1790 78 267 21 574 1135 681
Cuts at: 5591 5782 826
Size: 191 1471
Fragments arranged by size:
1790 1471 1135 681 574 267 219 191
78 21
SfoI GGC'GCC
Cuts at: 2567 2567
Size: 6427
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 3754 1872
Size: 1882 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3376 4531 5432 5694 820
Size: 197 926 1433 1155 901 262 1553
Fragments arranged by size:
1553 1433 1155 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 3452 3785 1132
Size: 2320 333 3774
Fragments arranged by size:
3774 2320 333
SspI AAT'ATT
Cuts at: 5 52 3345 4351 5
Size: 47 3293 1006 2081
Fragments arranged by size:
3293 2081 1006 47
StuI AGG'CCT
Cuts at: 1517 2841 1517
Size: 1324 5103
StyI C'CwwG_G
Cuts at: 513 1065 2004 2583 2605 3119 3866 513
Size: 552 939 579 22 514 747 3074
Fragments arranged by size:
3074 939 747 579 552 514 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4454 4471 4624 4809 5254 325
Size: 1255 2874 17 153 185 445 1498
Fragments arranged by size:
2874 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2950 4673 6385
Size: 274 80 33 51 1443 973 1723 1712
Cuts at: 6385 96
Size: 138
Fragments arranged by size:
1723 1712 1443 973 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6387 6419 6387
Size: 32 6395
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6427
XbaI T'CTAG_A
Cuts at: 2957 3652 3763 2957
Size: 695 111 5621
Fragments arranged by size:
5621 695 111
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6427
XmaI C'CCGG_G
Cuts at: 1870 3752 1870
Size: 1882 4545
XmnI GAAnn'nnTTC
Cuts at: 4556 4556
Size: 6427
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4235 276
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I EcoICRI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI