Restriction map for
pFN21AE0978
(Circular) (Six-base) MAPSORT of: pFN21AE0978.seq Check: 7751 from: 1 to: 6352
pFN21AE0978 6352 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2364 2697 2988 3157 2364
Size: 333 291 169 5559
Fragments arranged by size:
5559 333 291 169
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4162 278
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3695 1937
Size: 1758 4594
Acc65I G'GTAC_C
Cuts at: 3673 3673
Size: 6352
AclI AA'CG_TT
Cuts at: 1563 4479 4852 1563
Size: 2916 373 3063
Fragments arranged by size:
3063 2916 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2931 3453 4415 5799 6067 1632
Size: 1299 522 962 1384 268 1917
Fragments arranged by size:
1917 1384 1299 962 522 268
AfeI AGC'GCT
Cuts at: 3201 3201
Size: 6352
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6155
AflIII A'CryG_T
Cuts at: 1203 2085 2266 5251 6228 1203
Size: 882 181 2985 977 1327
Fragments arranged by size:
2985 1327 977 882 181
AgeI A'CCGG_T
Cuts at: 5183 5183
Size: 6352
AhdI GACnn_n'nnGTC
Cuts at: 5081 5081
Size: 6352
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6352
AlwNI CAG_nnn'CTG
Cuts at: 2365 3020 4095 5667 2365
Size: 655 1075 1572 3050
Fragments arranged by size:
3050 1572 1075 655
ApaI G_GGCC'C
Cuts at: 3027 3027
Size: 6352
ApaLI G'TGCA_C
Cuts at: 4409 5565 4409
Size: 1156 5196
ApoI r'AATT_y
Cuts at: 1493 3661 3887 4039 6148 1493
Size: 2168 226 152 2109 1697
Fragments arranged by size:
2168 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4906 160
Size: 872 3874 1606
Fragments arranged by size:
3874 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
AvaI C'yCGr_G
Cuts at: 1870 1943 3030 3248 3503 3677 1870
Size: 73 1087 218 255 174 4545
Fragments arranged by size:
4545 1087 255 218 174 73
AvrII C'CTAG_G
Cuts at: 2846 2846
Size: 6352
BamHI G'GATC_C
Cuts at: 3682 4049 3682
Size: 367 5985
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2547 3567 3673 5128 618
Size: 325 217 627 760 1020 106 1455 1842
Fragments arranged by size:
1842 1455 1020 760 627 325 217 106
BanII G_rGCy'C
Cuts at: 1391 2438 2724 3027 3039 3321 3671 1391
Size: 1047 286 303 12 282 350 4072
Fragments arranged by size:
4072 1047 350 303 286 282 12
BbeI G_GCGC'C
Cuts at: 3571 3571
Size: 6352
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5381
BbvCI CC'TCA_GC
Cuts at: 2453 2453
Size: 6352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2452 2486 4543 4577 1903
Size: 34 515 34 2057 34 3678
Fragments arranged by size:
3678 2057 515 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4244 5454 4244
Size: 1210 5142
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6352
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3508 3565 4963
Size: 107 122 71 631 605 1836 57 1398
Cuts at: 4963 136
Size: 1525
Fragments arranged by size:
1836 1525 1398 631 605 122 107 71
57
BglII A'GATC_T
Cuts at: 2340 6347 2340
Size: 4007 2345
BlpI GC'TnA_GC
Cuts at: 2369 2432 2723 2757 3769 2369
Size: 63 291 34 1012 4952
Fragments arranged by size:
4952 1012 291 63 34
Bme1580I G_kGCm'C
Cuts at: 1790 3027 4413 5569 1790
Size: 1237 1386 1156 2573
Fragments arranged by size:
2573 1386 1237 1156
BmgBI CAC'GTC
Cuts at: 1318 2248 2291 1318
Size: 930 43 5379
Fragments arranged by size:
5379 930 43
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3316 4083 5041
Size: 416 53 83 399 24 1899 767 958
Cuts at: 5041 442
Size: 1753
Fragments arranged by size:
1899 1753 958 767 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6352
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 2693 2077
Size: 616 5736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2114 2207 2675 2858 3169 3224 3479
Size: 827 48 93 468 183 311 55 255
Cuts at: 3479 5012 1239
Size: 1533 2579
Fragments arranged by size:
2579 1533 827 468 311 255 183 93
55 48
Bpu10I CC'TnA_GC
Cuts at: 2052 2453 2615 2052
Size: 401 162 5789
Fragments arranged by size:
5789 401 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3587 4477 5342 5640 3587
Size: 890 865 298 4299
Fragments arranged by size:
4299 890 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3423 5015 882
Size: 617 1924 1592 2219
Fragments arranged by size:
2219 1924 1592 617
BsaAI yAC'GTr
Cuts at: 493 1204 2086 4143 493
Size: 711 882 2057 2702
Fragments arranged by size:
2702 2057 882 711
BsaBI GATnn'nnATC
Cuts at: 6346 6346
Size: 6352
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3568 4159 4541 5176
Size: 53 83 186 936 2035 591 382 635
Cuts at: 5176 275
Size: 1451
Fragments arranged by size:
2035 1451 936 635 591 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2513 4785 5183 5457 5604 6015 2513
Size: 2272 398 274 147 411 2850
Fragments arranged by size:
2850 2272 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2678 2708 3308 3338 1178
Size: 30 165 30 1275 30 600 30 4192
Fragments arranged by size:
4192 1275 600 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 2938 3176 1186
Size: 1165 587 238 4362
Fragments arranged by size:
4362 1165 587 238
BseYI C'CCAG_C
Cuts at: 2033 2387 2788 2873 3020 3489 3520 5555 6051
Size: 354 401 85 147 469 31 2035 496
Cuts at: 6051 2033
Size: 2334
Fragments arranged by size:
2334 2035 496 469 401 354 147 85
31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2510 2633 2966 3197 1163
Size: 573 774 123 333 231 4318
Fragments arranged by size:
4318 774 573 333 231 123
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 3802 4563 5591 1540
Size: 275 186 1126 675 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 675 275 186
BsiHKAI G_wGCw'C
Cuts at: 2438 4413 4498 5569 2438
Size: 1975 85 1071 3221
Fragments arranged by size:
3221 1975 1071 85
BsmI GAATG_Cn'
Cuts at: 3863 3956 3863
Size: 93 6259
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2438 2724 3027 3039 3321 3671 4413
Size: 399 648 286 303 12 282 350 742
Cuts at: 4413 4498 5569 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 648 399 350 303 286
282 85 12
BspEI T'CCGG_A
Cuts at: 6015 6015
Size: 6352
BspHI T'CATG_A
Cuts at: 4241 5971 4241
Size: 1730 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2331 2364 2697 2988 3157 3263 3264 3707
Size: 1487 33 333 291 169 106 1 443
Cuts at: 3707 844
Size: 3489
Fragments arranged by size:
3489 1487 443 333 291 169 106 33
1
BsrBI CCG'CTC
Cuts at: 1394 1823 2569 4239 1394
Size: 429 746 1670 3507
Fragments arranged by size:
3507 1670 746 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4847 5021 65
Size: 1183 3599 174 1396
Fragments arranged by size:
3599 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3059 3122 4996 5183 1700
Size: 110 1249 63 1874 187 2869
Fragments arranged by size:
2869 1874 1249 187 110 63
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6352
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6352
BssSI C'ACGA_G
Cuts at: 3215 4412 5424 3215
Size: 1197 1012 4143
Fragments arranged by size:
4143 1197 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2365 3226 794
Size: 1571 861 3920
Fragments arranged by size:
3920 1571 861
BstBI TT'CG_AA
Cuts at: 5156 5156
Size: 6352
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6352
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2816 2011
Size: 805 5547
BstYI r'GATC_y
Cuts at: 1972 2172 2340 3682 4049 4434 4451 5903 5989
Size: 200 168 1342 367 385 17 1452 86
Cuts at: 5989 6001 6347 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1342 385 367 346 200 168
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2408 2803 3051 2408
Size: 395 248 5709
Fragments arranged by size:
5709 395 248
BtgI C'CryG_G
Cuts at: 513 1065 2257 2442 3339 513
Size: 552 1192 185 897 3526
Fragments arranged by size:
3526 1192 897 552 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2208 487
Size: 683 56 982 4631
Fragments arranged by size:
4631 982 683 56
BtsI GCAGTG_nn'
Cuts at: 2380 3182 3868 4662 4682 2380
Size: 802 686 794 20 4050
Fragments arranged by size:
4050 802 794 686 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6317
DraI TTT'AAA
Cuts at: 3656 3656
Size: 6352
DraIII CAC_nnn'GTG
Cuts at: 2557 2557
Size: 6352
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5359 809
Size: 507 4043 1802
Fragments arranged by size:
4043 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2818 2974
Size: 54 1425 50 275 18 333 655 156
Cuts at: 2974 3004 3082 3124 3298 3799 4688 6317 8
Size: 30 78 42 174 501 889 1629 43
Fragments arranged by size:
1629 1425 889 655 501 333 275 174
156 78 54 50 43 42 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 3799 1537
Size: 275 1312 675 4090
Fragments arranged by size:
4090 1312 675 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2528 4282 1353
Size: 1175 1754 3423
Fragments arranged by size:
3423 1754 1175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4939 5313 5459 387
Size: 1064 138 3350 374 146 1280
Fragments arranged by size:
3350 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2436 2436
Size: 6352
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2114 2207 2675 2858 2931 3169
Size: 393 434 48 93 468 183 73 238
Cuts at: 3169 3224 3453 3479 4415 5012 5799 6067 1239
Size: 55 229 26 936 597 787 268 1524
Fragments arranged by size:
1524 936 787 597 468 434 393 268
238 229 183 93 73 55 48 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2960 3266 2960
Size: 306 6046
EcoO109I rG'GnC_Cy
Cuts at: 2503 2520 2881 2965 3024 3238 2503
Size: 17 361 84 59 214 5617
Fragments arranged by size:
5617 361 214 84 59 17
EcoRI G'AATT_C
Cuts at: 3661 4039 3661
Size: 378 5974
FseI GG_CCGG'CC
Cuts at: 3063 3126 3063
Size: 63 6289
FspI TGC'GCA
Cuts at: 4858 4858
Size: 6352
HaeII r_GCGC'y
Cuts at: 1678 3203 3212 3360 3571 5499 1678
Size: 1525 9 148 211 1928 2531
Fragments arranged by size:
2531 1928 1525 211 148 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2740 2772 2870 2902 4993
Size: 32 135 32 1168 32 98 32 2091
Cuts at: 4993 5025 5067 5099 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2091 1168 135 98 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2272 3696 3942 669
Size: 988 281 334 1424 246 3079
Fragments arranged by size:
3079 1424 988 334 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6352
HpaI GTT'AAC
Cuts at: 3942 3942
Size: 6352
KasI G'GCGC_C
Cuts at: 3567 3567
Size: 6352
KpnI G_GTAC'C
Cuts at: 3677 3677
Size: 6352
MfeI C'AATT_G
Cuts at: 3951 3951
Size: 6352
MluI A'CGCG_T
Cuts at: 6228 6228
Size: 6352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5466 5650 1305
Size: 4161 184 2007
Fragments arranged by size:
4161 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2820 2976 3006 3300 10
Size: 54 1425 343 988 156 30 294 3062
Fragments arranged by size:
3062 1425 988 343 294 156 54 30
MslI CAynn'nnrTG
Cuts at: 518 1166 4310 4669 4828 518
Size: 648 3144 359 159 2042
Fragments arranged by size:
3144 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2362 2452 2494 3499 3524 3768 4445 5593
Size: 756 90 42 1005 25 244 677 1148
Cuts at: 5593 5838 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1005 756 677 245 244 90
42 25
NaeI GCC'GGC
Cuts at: 3061 3124 3061
Size: 63 6289
NarI GG'CG_CC
Cuts at: 3568 3568
Size: 6352
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5800
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6352
NgoMIV G'CCGG_C
Cuts at: 3059 3122 3059
Size: 63 6289
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6352
NotI GC'GGCC_GC
Cuts at: 3799 3799
Size: 6352
NspI r_CATG'y
Cuts at: 1132 1229 2663 3710 5255 1132
Size: 97 1434 1047 1545 2229
Fragments arranged by size:
2229 1545 1434 1047 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6352
PciI A'CATG_T
Cuts at: 5251 5251
Size: 6352
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3633 1108
Size: 2525 3827
PfoI T'CCnGG_A
Cuts at: 2499 3261 2499
Size: 762 5590
PmeI GTTT'AAAC
Cuts at: 3656 3656
Size: 6352
PmlI CAC'GTG
Cuts at: 2086 2086
Size: 6352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4421 4453 5934 5966 4421
Size: 32 1481 32 4807
Fragments arranged by size:
4807 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2503 2520 3238 2503
Size: 17 718 5617
Fragments arranged by size:
5617 718 17
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6352
PsiI TTA'TAA
Cuts at: 3922 3922
Size: 6352
PspOMI G'GGCC_C
Cuts at: 3023 3023
Size: 6352
PstI C_TGCA'G
Cuts at: 830 2361 2465 3552 3704 830
Size: 1531 104 1087 152 3478
Fragments arranged by size:
3478 1531 1087 152 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6352
PvuII CAG'CTG
Cuts at: 2452 2494 3499 2452
Size: 42 1005 5305
Fragments arranged by size:
5305 1005 42
SacI G_AGCT'C
Cuts at: 2438 2438
Size: 6352
SalI G'TCGA_C
Cuts at: 1936 3694 1936
Size: 1758 4594
SapI GCTCTTCn'nnn_
Cuts at: 2528 2528
Size: 6352
SbfI CC_TGCA'GG
Cuts at: 3704 3704
Size: 6352
ScaI AGT'ACT
Cuts at: 4600 6312 4600
Size: 1712 4640
SfcI C'TryA_G
Cuts at: 826 1045 2357 2461 3352 3548 3700 4835 5516
Size: 219 1312 104 891 196 152 1135 681
Cuts at: 5516 5707 826
Size: 191 1471
Fragments arranged by size:
1471 1312 1135 891 681 219 196 191
152 104
SfoI GGC'GCC
Cuts at: 3569 3569
Size: 6352
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6352
SmaI CCC'GGG
Cuts at: 1872 3250 3679 1872
Size: 1378 429 4545
Fragments arranged by size:
4545 1378 429
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3602 4456 5357 5619 820
Size: 197 926 1659 854 901 262 1553
Fragments arranged by size:
1659 1553 926 901 854 262 197
SnaBI TAC'GTA
Cuts at: 493 4143 493
Size: 3650 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6352
SphI G_CATG'C
Cuts at: 1132 3710 1132
Size: 2578 3774
SspI AAT'ATT
Cuts at: 5 52 4276 5
Size: 47 4224 2081
Fragments arranged by size:
4224 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6352
StyI C'CwwG_G
Cuts at: 513 1065 2846 3791 513
Size: 552 1781 945 3074
Fragments arranged by size:
3074 1781 945 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3359 3503 4379 4396 4549 4734 5179
Size: 1255 1779 144 876 17 153 185 445
Cuts at: 5179 325
Size: 1498
Fragments arranged by size:
1779 1498 1255 876 445 185 153 144
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4598 6310 96
Size: 274 80 33 51 1443 2621 1712 138
Fragments arranged by size:
2621 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2577 2609 2678 2710 3031 3063 3308 3340 6312
Size: 32 69 32 321 32 245 32 2972
Cuts at: 6312 6344 2577
Size: 32 2585
Fragments arranged by size:
2972 2585 321 245 69 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6352
XbaI T'CTAG_A
Cuts at: 3688 3688
Size: 6352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2041 2234 2551 2011
Size: 30 193 317 5812
Fragments arranged by size:
5812 317 193 30
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6352
XmaI C'CCGG_G
Cuts at: 1870 3248 3677 1870
Size: 1378 429 4545
Fragments arranged by size:
4545 1378 429
XmnI GAAnn'nnTTC
Cuts at: 4481 4481
Size: 6352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4160 276
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV
FalI FspAI NruI NsiI PacI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SrfI SwaI