Restriction map for
pFN21AE0977
(Circular) (Six-base) MAPSORT of: pFN21AE0977.seq Check: 3301 from: 1 to: 6418
pFN21AE0977 6418 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4228 278
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3761 1937
Size: 1824 4594
Acc65I G'GTAC_C
Cuts at: 3739 3739
Size: 6418
AclI AA'CG_TT
Cuts at: 1563 4545 4918 1563
Size: 2982 373 3063
Fragments arranged by size:
3063 2982 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2235 4481 5865 6133 1632
Size: 603 2246 1384 268 1917
Fragments arranged by size:
2246 1917 1384 603 268
AflII C'TTAA_G
Cuts at: 820 1017 2261 820
Size: 197 1244 4977
Fragments arranged by size:
4977 1244 197
AflIII A'CryG_T
Cuts at: 1203 2047 2165 5317 6294 1203
Size: 844 118 3152 977 1327
Fragments arranged by size:
3152 1327 977 844 118
AgeI A'CCGG_T
Cuts at: 5249 5249
Size: 6418
AhdI GACnn_n'nnGTC
Cuts at: 5147 5147
Size: 6418
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3707 1184
Size: 2523 3895
AlwNI CAG_nnn'CTG
Cuts at: 4161 5733 4161
Size: 1572 4846
ApaI G_GGCC'C
Cuts at: 3309 3450 3309
Size: 141 6277
ApaLI G'TGCA_C
Cuts at: 4475 5631 4475
Size: 1156 5262
ApoI r'AATT_y
Cuts at: 1493 2023 2797 2937 3727 3953 4105 6214 1493
Size: 530 774 140 790 226 152 2109 1697
Fragments arranged by size:
2109 1697 790 774 530 226 152 140
AseI AT'TA_AT
Cuts at: 160 1032 4972 160
Size: 872 3940 1606
Fragments arranged by size:
3940 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
AvaI C'yCGr_G
Cuts at: 1870 1943 3743 1870
Size: 73 1800 4545
Fragments arranged by size:
4545 1800 73
BamHI G'GATC_C
Cuts at: 2887 3748 4115 2887
Size: 861 367 5190
Fragments arranged by size:
5190 861 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2674 3739 5194 618
Size: 325 217 627 887 1065 1455 1842
Fragments arranged by size:
1842 1455 1065 887 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2140 2700 3309 3342 3450 3737 1391
Size: 749 560 609 33 108 287 4072
Fragments arranged by size:
4072 749 609 560 287 108 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2930 928
Size: 971 1031 4416
Fragments arranged by size:
4416 1031 971
BbvCI CC'TCA_GC
Cuts at: 2495 2495
Size: 6418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4609 4643 1903
Size: 34 2672 34 3678
Fragments arranged by size:
3678 2672 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4310 5520 4310
Size: 1210 5208
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6418
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3536 5029 136
Size: 107 122 71 631 605 1864 1493 1525
Fragments arranged by size:
1864 1525 1493 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6413 6413
Size: 6418
BlpI GC'TnA_GC
Cuts at: 3835 3835
Size: 6418
Bme1580I G_kGCm'C
Cuts at: 1790 3309 3450 4479 5635 1790
Size: 1519 141 1029 1156 2573
Fragments arranged by size:
2573 1519 1156 1029 141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6418
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4149 5107 442
Size: 416 53 83 399 24 2732 958 1753
Fragments arranged by size:
2732 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2951 3542 3645 5078 1239
Size: 1712 591 103 1433 2579
Fragments arranged by size:
2579 1712 1433 591 103
Bpu10I CC'TnA_GC
Cuts at: 2495 2495
Size: 6418
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3455 3510 4543 5408 5706 3455
Size: 55 1033 865 298 4167
Fragments arranged by size:
4167 1033 865 298 55
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5081 882
Size: 617 3582 2219
Fragments arranged by size:
3582 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2048 3137 4209 493
Size: 711 844 1089 1072 2702
Fragments arranged by size:
2702 1089 1072 844 711
BsaBI GATnn'nnATC
Cuts at: 6412 6412
Size: 6418
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4225 4607 5242 275
Size: 53 83 186 936 2692 382 635 1451
Fragments arranged by size:
2692 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2773 4851 5249 5523 5670 6081 2773
Size: 2078 398 274 147 411 3110
Fragments arranged by size:
3110 2078 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6193
Fragments arranged by size:
6193 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2100 3638 1186
Size: 914 1538 3966
Fragments arranged by size:
3966 1538 914
BseYI C'CCAG_C
Cuts at: 3260 5621 6117 3260
Size: 2361 496 3561
Fragments arranged by size:
3561 2361 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5845
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3868 4629 5657 1540
Size: 275 186 1867 761 1028 2301
Fragments arranged by size:
2301 1867 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2140 3217 4479 4564 5635 2140
Size: 1077 1262 85 1071 2923
Fragments arranged by size:
2923 1262 1077 1071 85
BsmI GAATG_Cn'
Cuts at: 3929 4022 3929
Size: 93 6325
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2700 3217 3309 3342 3450 3737
Size: 399 350 560 517 92 33 108 287
Cuts at: 3737 4479 4564 5635 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 560 517 399 350 287
108 92 85 33
BspEI T'CCGG_A
Cuts at: 2773 6081 2773
Size: 3308 3110
BspHI T'CATG_A
Cuts at: 2316 4307 6037 2316
Size: 1991 1730 2697
Fragments arranged by size:
2697 1991 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2448 3264 3773 844
Size: 1604 816 509 3489
Fragments arranged by size:
3489 1604 816 509
BsrBI CCG'CTC
Cuts at: 1394 1823 4305 1394
Size: 429 2482 3507
Fragments arranged by size:
3507 2482 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4913 5087 65
Size: 1183 3665 174 1396
Fragments arranged by size:
3665 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3165 3360 5062 5249 1700
Size: 110 1355 195 1702 187 2869
Fragments arranged by size:
2869 1702 1355 195 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6418
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6418
BssSI C'ACGA_G
Cuts at: 3171 4478 5490 3171
Size: 1307 1012 4099
Fragments arranged by size:
4099 1307 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6418
BstBI TT'CG_AA
Cuts at: 5222 5222
Size: 6418
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6418
BstYI r'GATC_y
Cuts at: 1972 2613 2887 3054 3489 3748 4115 4500 4517
Size: 641 274 167 435 259 367 385 17
Cuts at: 4517 5969 6055 6067 6413 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 641 435 385 367 346 274
259 167 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2345 2679 3679 2345
Size: 334 1000 5084
Fragments arranged by size:
5084 1000 334
BtgI C'CryG_G
Cuts at: 513 1065 2004 2649 513
Size: 552 939 645 4282
Fragments arranged by size:
4282 939 645 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2737 487
Size: 683 56 1511 4168
Fragments arranged by size:
4168 1511 683 56
BtsI GCAGTG_nn'
Cuts at: 2493 2589 3934 4728 4748 2493
Size: 96 1345 794 20 4163
Fragments arranged by size:
4163 1345 794 96 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6418
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6383
DraI TTT'AAA
Cuts at: 3722 3722
Size: 6418
DraIII CAC_nnn'GTG
Cuts at: 2833 2833
Size: 6418
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5425 809
Size: 507 4109 1802
Fragments arranged by size:
4109 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2120 2646 3167
Size: 54 1425 50 275 18 290 526 521
Cuts at: 3167 3865 4754 6383 8
Size: 698 889 1629 43
Fragments arranged by size:
1629 1425 889 698 526 521 290 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3865 1537
Size: 275 2053 4090
Fragments arranged by size:
4090 2053 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2058 2107 2955 4348 1353
Size: 705 49 848 1393 3423
Fragments arranged by size:
3423 1393 848 705 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5005 5379 5525 387
Size: 1064 138 3416 374 146 1280
Fragments arranged by size:
3416 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2138 2138
Size: 6418
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2235 2951 3542 3645 4481 5078 5865
Size: 393 603 716 591 103 836 597 787
Cuts at: 5865 6133 1239
Size: 268 1524
Fragments arranged by size:
1524 836 787 716 603 597 591 393
268 103
EcoO109I rG'GnC_Cy
Cuts at: 2530 3223 3305 3447 3585 2530
Size: 693 82 142 138 5363
Fragments arranged by size:
5363 693 142 138 82
EcoRI G'AATT_C
Cuts at: 2023 3727 4105 2023
Size: 1704 378 4336
Fragments arranged by size:
4336 1704 378
EcoRV GAT'ATC
Cuts at: 3115 3115
Size: 6418
FspI TGC'GCA
Cuts at: 4924 4924
Size: 6418
HaeII r_GCGC'y
Cuts at: 1678 5565 1678
Size: 3887 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2522 2554 2708 2740 3072
Size: 32 135 32 950 32 154 32 332
Cuts at: 3072 3104 5059 5091 5133 5165 1373
Size: 32 1955 32 42 32 2626
Fragments arranged by size:
2626 1955 950 332 154 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3202 3762 4008 669
Size: 988 281 1264 560 246 3079
Fragments arranged by size:
3079 1264 988 560 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6418
HpaI GTT'AAC
Cuts at: 4008 4008
Size: 6418
KpnI G_GTAC'C
Cuts at: 3743 3743
Size: 6418
MfeI C'AATT_G
Cuts at: 2028 2378 4017 2028
Size: 350 1639 4429
Fragments arranged by size:
4429 1639 350
MluI A'CGCG_T
Cuts at: 2165 6294 2165
Size: 4129 2289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5532 5716 1305
Size: 4227 184 2007
Fragments arranged by size:
4227 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2122 10
Size: 54 1425 343 290 4306
Fragments arranged by size:
4306 1425 343 290 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 4376 4735 4894 518
Size: 648 1320 1890 359 159 2042
Fragments arranged by size:
2042 1890 1320 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3521 3834 4511 5659 5904 1606
Size: 1915 313 677 1148 245 2120
Fragments arranged by size:
2120 1915 1148 677 313 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4927
Fragments arranged by size:
4927 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6418
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6418
NotI GC'GGCC_GC
Cuts at: 3865 3865
Size: 6418
NspI r_CATG'y
Cuts at: 1132 1229 2299 2387 3776 5321 1132
Size: 97 1070 88 1389 1545 2229
Fragments arranged by size:
2229 1545 1389 1070 97 88
PasI CC'CwG_GG
Cuts at: 1348 3589 3700 1348
Size: 2241 111 4066
Fragments arranged by size:
4066 2241 111
PciI A'CATG_T
Cuts at: 5317 5317
Size: 6418
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2830 3176 1108
Size: 1722 346 4350
Fragments arranged by size:
4350 1722 346
PfoI T'CCnGG_A
Cuts at: 2408 3558 2408
Size: 1150 5268
PmeI GTTT'AAAC
Cuts at: 3722 3722
Size: 6418
PmlI CAC'GTG
Cuts at: 2048 2048
Size: 6418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4487 4519 6000 6032 4487
Size: 32 1481 32 4873
Fragments arranged by size:
4873 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2530 3223 2530
Size: 693 5725
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6418
PsiI TTA'TAA
Cuts at: 2144 3988 2144
Size: 1844 4574
PspOMI G'GGCC_C
Cuts at: 3305 3446 3305
Size: 141 6277
PstI C_TGCA'G
Cuts at: 830 2584 2988 3770 830
Size: 1754 404 782 3478
Fragments arranged by size:
3478 1754 782 404
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6418
PvuII CAG'CTG
Cuts at: 3521 3521
Size: 6418
SacI G_AGCT'C
Cuts at: 2140 2140
Size: 6418
SalI G'TCGA_C
Cuts at: 1936 3760 1936
Size: 1824 4594
SapI GCTCTTCn'nnn_
Cuts at: 2955 2955
Size: 6418
SbfI CC_TGCA'GG
Cuts at: 3770 3770
Size: 6418
ScaI AGT'ACT
Cuts at: 4666 6378 4666
Size: 1712 4706
SfcI C'TryA_G
Cuts at: 826 1045 2580 2951 2984 3766 4901 5582 5773
Size: 219 1535 371 33 782 1135 681 191
Cuts at: 5773 826
Size: 1471
Fragments arranged by size:
1535 1471 1135 782 681 371 219 191
33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
SgrAI Cr'CCGG_yG
Cuts at: 1700 3360 1700
Size: 1660 4758
SmaI CCC'GGG
Cuts at: 1872 3745 1872
Size: 1873 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2261 3470 3525 4522 5423 5685
Size: 197 926 318 1209 55 997 901 262
Cuts at: 5685 820
Size: 1553
Fragments arranged by size:
1553 1209 997 926 901 318 262 197
55
SnaBI TAC'GTA
Cuts at: 493 4209 493
Size: 3716 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6418
SphI G_CATG'C
Cuts at: 1132 2387 3776 1132
Size: 1255 1389 3774
Fragments arranged by size:
3774 1389 1255
SspI AAT'ATT
Cuts at: 5 52 4342 5
Size: 47 4290 2081
Fragments arranged by size:
4290 2081 47
StuI AGG'CCT
Cuts at: 1517 3690 1517
Size: 2173 4245
StyI C'CwwG_G
Cuts at: 513 1065 2004 2450 3857 513
Size: 552 939 446 1407 3074
Fragments arranged by size:
3074 1407 939 552 446
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2345 4445 4462 4615 4800 5245 325
Size: 1255 765 2100 17 153 185 445 1498
Fragments arranged by size:
2100 1498 1255 765 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2308 3474 4664
Size: 274 80 33 51 1443 331 1166 1190
Cuts at: 4664 6376 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1190 1166 331 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6378 6410 6378
Size: 32 6386
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6418
XbaI T'CTAG_A
Cuts at: 3754 3754
Size: 6418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3004 3566 3004
Size: 562 5856
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6418
XmaI C'CCGG_G
Cuts at: 1870 3743 1870
Size: 1873 4545
XmnI GAAnn'nnTTC
Cuts at: 4547 4547
Size: 6418
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4226 276
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI