Restriction map for
pFN21AE0971
(Circular) (Six-base) MAPSORT of: pFN21AE0971.seq Check: 8132 from: 1 to: 6325
pFN21AE0971 6325 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2157 2157
Size: 6325
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4135 278
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3668 1937
Size: 1731 4594
Acc65I G'GTAC_C
Cuts at: 3646 3646
Size: 6325
AclI AA'CG_TT
Cuts at: 1563 4452 4825 1563
Size: 2889 373 3063
Fragments arranged by size:
3063 2889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3234 3539 4388 5772 6040 1632
Size: 1602 305 849 1384 268 1917
Fragments arranged by size:
1917 1602 1384 849 305 268
AflII C'TTAA_G
Cuts at: 820 1017 2194 820
Size: 197 1177 4951
Fragments arranged by size:
4951 1177 197
AflIII A'CryG_T
Cuts at: 1203 5224 6201 1203
Size: 4021 977 1327
Fragments arranged by size:
4021 1327 977
AgeI A'CCGG_T
Cuts at: 5156 5156
Size: 6325
AhdI GACnn_n'nnGTC
Cuts at: 5054 5054
Size: 6325
AleI CACnn'nnGTG
Cuts at: 1166 2973 1166
Size: 1807 4518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2048 2403 1184
Size: 864 355 5106
Fragments arranged by size:
5106 864 355
AlwNI CAG_nnn'CTG
Cuts at: 4068 5640 4068
Size: 1572 4753
ApaLI G'TGCA_C
Cuts at: 4382 5538 4382
Size: 1156 5169
ApoI r'AATT_y
Cuts at: 1493 2208 2253 2270 2474 3503 3601 3634 3860
Size: 715 45 17 204 1029 98 33 226
Cuts at: 3860 4012 6121 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1029 715 226 204 152 98
45 33 17
AseI AT'TA_AT
Cuts at: 160 1032 2754 4879 160
Size: 872 1722 2125 1606
Fragments arranged by size:
2125 1722 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
AvaI C'yCGr_G
Cuts at: 1870 1943 3650 1870
Size: 73 1707 4545
Fragments arranged by size:
4545 1707 73
BamHI G'GATC_C
Cuts at: 3044 3321 3655 4022 3044
Size: 277 334 367 5347
Fragments arranged by size:
5347 367 334 277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 3646 5101 618
Size: 325 217 627 297 1562 1455 1842
Fragments arranged by size:
1842 1562 1455 627 325 297 217
BanII G_rGCy'C
Cuts at: 1391 3644 1391
Size: 2253 4072
BbeI G_GCGC'C
Cuts at: 2088 2088
Size: 6325
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2453 3345 3545 928
Size: 971 554 892 200 3708
Fragments arranged by size:
3708 971 892 554 200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4516 4550 1903
Size: 34 2579 34 3678
Fragments arranged by size:
3678 2579 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4217 5427 4217
Size: 1210 5115
BclI T'GATC_A
Cuts at: 1548 3226 3408 1548
Size: 1678 182 4465
Fragments arranged by size:
4465 1678 182
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4936 136
Size: 107 122 71 631 605 3264 1525
Fragments arranged by size:
3264 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6320 6320
Size: 6325
BlpI GC'TnA_GC
Cuts at: 3742 3742
Size: 6325
Bme1580I G_kGCm'C
Cuts at: 1790 2827 4386 5542 1790
Size: 1037 1559 1156 2573
Fragments arranged by size:
2573 1559 1156 1037
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6325
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4056 5014 442
Size: 416 53 83 399 24 2639 958 1753
Fragments arranged by size:
2639 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4985 1239
Size: 3746 2579
Bpu10I CC'TnA_GC
Cuts at: 2075 2075
Size: 6325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3090 3120 4450 5315 5613 3090
Size: 30 1330 865 298 3802
Fragments arranged by size:
3802 1330 865 298 30
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4988 882
Size: 617 3489 2219
Fragments arranged by size:
3489 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2795 4116 493
Size: 711 1591 1321 2702
Fragments arranged by size:
2702 1591 1321 711
BsaBI GATnn'nnATC
Cuts at: 3592 6319 3592
Size: 2727 3598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 4132 4514 5149
Size: 53 83 186 936 552 2047 382 635
Cuts at: 5149 275
Size: 1451
Fragments arranged by size:
2047 1451 936 635 552 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4758 5156 5430 5577 5988 4758
Size: 398 274 147 411 5095
Fragments arranged by size:
5095 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6100
Fragments arranged by size:
6100 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2412 3093 1186
Size: 1226 681 4418
Fragments arranged by size:
4418 1226 681
BseYI C'CCAG_C
Cuts at: 3312 5528 6024 3312
Size: 2216 496 3613
Fragments arranged by size:
3613 2216 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 2393 2538 1163
Size: 573 399 258 145 4950
Fragments arranged by size:
4950 573 399 258 145
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3775 4536 5564 1540
Size: 275 186 1774 761 1028 2301
Fragments arranged by size:
2301 1774 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2794 4386 4471 5542 2794
Size: 1592 85 1071 3577
Fragments arranged by size:
3577 1592 1071 85
BsmI GAATG_Cn'
Cuts at: 3218 3836 3929 3218
Size: 618 93 5614
Fragments arranged by size:
5614 618 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2794 2827 3644 4386 4471 5542 1391
Size: 399 1004 33 817 742 85 1071 2174
Fragments arranged by size:
2174 1071 1004 817 742 399 85 33
BspEI T'CCGG_A
Cuts at: 5988 5988
Size: 6325
BspHI T'CATG_A
Cuts at: 2437 3411 4214 5944 2437
Size: 974 803 1730 2818
Fragments arranged by size:
2818 1730 974 803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2073 2157 2415 3680 844
Size: 1229 84 258 1265 3489
Fragments arranged by size:
3489 1265 1229 258 84
BsrBI CCG'CTC
Cuts at: 1394 1823 4212 1394
Size: 429 2389 3507
Fragments arranged by size:
3507 2389 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2266 4820 4994 65
Size: 1183 1018 2554 174 1396
Fragments arranged by size:
2554 1396 1183 1018 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4969 5156 1700
Size: 110 3159 187 2869
Fragments arranged by size:
3159 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6325
BssSI C'ACGA_G
Cuts at: 4385 5397 4385
Size: 1012 5313
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6325
BstBI TT'CG_AA
Cuts at: 5129 5129
Size: 6325
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6325
BstYI r'GATC_y
Cuts at: 1972 3044 3203 3321 3655 4022 4407 4424 5876
Size: 1072 159 118 334 367 385 17 1452
Cuts at: 5876 5962 5974 6320 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1072 385 367 346 334 159
118 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2080 513
Size: 552 1015 4758
Fragments arranged by size:
4758 1015 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5586
Fragments arranged by size:
5586 683 56
BtsI GCAGTG_nn'
Cuts at: 3323 3841 4635 4655 3323
Size: 518 794 20 4993
Fragments arranged by size:
4993 794 518 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6290
DraI TTT'AAA
Cuts at: 3600 3629 3600
Size: 29 6296
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2126 5332 809
Size: 507 810 3206 1802
Fragments arranged by size:
3206 1802 810 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2357 3772
Size: 54 1425 50 275 18 306 221 1415
Cuts at: 3772 4661 6290 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1415 889 306 275 221 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3772 1537
Size: 275 1960 4090
Fragments arranged by size:
4090 1960 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2892 3253 4255 1353
Size: 1539 361 1002 3423
Fragments arranged by size:
3423 1539 1002 361
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2095 4912 5286 5432 387
Size: 1064 138 506 2817 374 146 1280
Fragments arranged by size:
2817 1280 1064 506 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3234 3539 4388 4985 5772 6040 1239
Size: 393 1602 305 849 597 787 268 1524
Fragments arranged by size:
1602 1524 849 787 597 393 305 268
EcoO109I rG'GnC_Cy
Cuts at: 3090 3453 3090
Size: 363 5962
EcoRI G'AATT_C
Cuts at: 3634 4012 3634
Size: 378 5947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2865 3386 2209
Size: 656 521 5148
Fragments arranged by size:
5148 656 521
FspI TGC'GCA
Cuts at: 4831 4831
Size: 6325
HaeII r_GCGC'y
Cuts at: 1678 2031 2088 5472 1678
Size: 353 57 3384 2531
Fragments arranged by size:
3384 2531 353 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2358 2390 3107 3139 4966
Size: 32 135 32 786 32 717 32 1827
Cuts at: 4966 4998 5040 5072 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1827 786 717 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3669 3915 669
Size: 988 281 1731 246 3079
Fragments arranged by size:
3079 1731 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6325
HpaI GTT'AAC
Cuts at: 3915 3915
Size: 6325
KasI G'GCGC_C
Cuts at: 2084 2084
Size: 6325
KpnI G_GTAC'C
Cuts at: 3650 3650
Size: 6325
MfeI C'AATT_G
Cuts at: 3924 3924
Size: 6325
MluI A'CGCG_T
Cuts at: 6201 6201
Size: 6325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5439 5623 1305
Size: 4134 184 2007
Fragments arranged by size:
4134 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2359 10
Size: 54 1425 343 527 3976
Fragments arranged by size:
3976 1425 527 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2973 4283 4642 4801 518
Size: 648 1807 1310 359 159 2042
Fragments arranged by size:
2042 1807 1310 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2082 2167 3741 4418 5566 5811 1606
Size: 476 85 1574 677 1148 245 2120
Fragments arranged by size:
2120 1574 1148 677 476 245 85
NarI GG'CG_CC
Cuts at: 2085 2085
Size: 6325
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5773
NdeI CA'TA_TG
Cuts at: 387 2773 387
Size: 2386 3939
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6325
NotI GC'GGCC_GC
Cuts at: 3772 3772
Size: 6325
NspI r_CATG'y
Cuts at: 1132 1229 2299 3683 5228 1132
Size: 97 1070 1384 1545 2229
Fragments arranged by size:
2229 1545 1384 1070 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6325
PciI A'CATG_T
Cuts at: 5224 5224
Size: 6325
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6325
PfoI T'CCnGG_A
Cuts at: 3169 3169
Size: 6325
PmeI GTTT'AAAC
Cuts at: 3629 3629
Size: 6325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4394 4426 5907 5939 4394
Size: 32 1481 32 4780
Fragments arranged by size:
4780 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3090 3453 3090
Size: 363 5962
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6325
PsiI TTA'TAA
Cuts at: 3895 3895
Size: 6325
PstI C_TGCA'G
Cuts at: 830 2027 3280 3300 3677 830
Size: 1197 1253 20 377 3478
Fragments arranged by size:
3478 1253 1197 377 20
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6325
SacII CC_GC'GG
Cuts at: 2083 2083
Size: 6325
SalI G'TCGA_C
Cuts at: 1936 3667 1936
Size: 1731 4594
SapI GCTCTTCn'nnn_
Cuts at: 3253 3253
Size: 6325
SbfI CC_TGCA'GG
Cuts at: 3677 3677
Size: 6325
ScaI AGT'ACT
Cuts at: 4573 6285 4573
Size: 1712 4613
SfcI C'TryA_G
Cuts at: 826 1045 2023 3276 3296 3673 4808 5489 5680
Size: 219 978 1253 20 377 1135 681 191
Cuts at: 5680 826
Size: 1471
Fragments arranged by size:
1471 1253 1135 978 681 377 219 191
20
SfoI GGC'GCC
Cuts at: 2086 2086
Size: 6325
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6325
SmaI CCC'GGG
Cuts at: 1872 3652 1872
Size: 1780 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2194 3105 3135 4429 5330 5592
Size: 197 926 251 911 30 1294 901 262
Cuts at: 5592 820
Size: 1553
Fragments arranged by size:
1553 1294 926 911 901 262 251 197
30
SnaBI TAC'GTA
Cuts at: 493 4116 493
Size: 3623 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6325
SphI G_CATG'C
Cuts at: 1132 3683 1132
Size: 2551 3774
SspI AAT'ATT
Cuts at: 5 52 4249 5
Size: 47 4197 2081
Fragments arranged by size:
4197 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6325
StyI C'CwwG_G
Cuts at: 513 1065 2392 3493 3764 513
Size: 552 1327 1101 271 3074
Fragments arranged by size:
3074 1327 1101 552 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4352 4369 4522 4707 5152 325
Size: 1255 2772 17 153 185 445 1498
Fragments arranged by size:
2772 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3520 4571 6283
Size: 274 80 33 51 1443 1543 1051 1712
Cuts at: 6283 96
Size: 138
Fragments arranged by size:
1712 1543 1443 1051 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6285 6317 6285
Size: 32 6293
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6325
XbaI T'CTAG_A
Cuts at: 3125 3584 3661 3125
Size: 459 77 5789
Fragments arranged by size:
5789 459 77
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3065 3065
Size: 6325
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6325
XmaI C'CCGG_G
Cuts at: 1870 3650 1870
Size: 1780 4545
XmnI GAAnn'nnTTC
Cuts at: 4454 4454
Size: 6325
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4133 276
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SanDI SexAI SfiI SrfI SwaI