Restriction map for
pFN21AE0967
(Circular) (Six-base) MAPSORT of: pFN21AE0967.seq Check: 8743 from: 1 to: 6244
pFN21AE0967 6244 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4054 278
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2709 3587 1937
Size: 772 878 4594
Fragments arranged by size:
4594 878 772
Acc65I G'GTAC_C
Cuts at: 3086 3565 3086
Size: 479 5765
AclI AA'CG_TT
Cuts at: 1563 4371 4744 1563
Size: 2808 373 3063
Fragments arranged by size:
3063 2808 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2040 2319 4307 5691 5959 1632
Size: 408 279 1988 1384 268 1917
Fragments arranged by size:
1988 1917 1384 408 279 268
AflII C'TTAA_G
Cuts at: 820 1017 2840 820
Size: 197 1823 4224
Fragments arranged by size:
4224 1823 197
AflIII A'CryG_T
Cuts at: 1203 3237 5143 6120 1203
Size: 2034 1906 977 1327
Fragments arranged by size:
2034 1906 1327 977
AgeI A'CCGG_T
Cuts at: 5075 5075
Size: 6244
AhdI GACnn_n'nnGTC
Cuts at: 2683 4973 2683
Size: 2290 3954
AleI CACnn'nnGTG
Cuts at: 1166 3503 1166
Size: 2337 3907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2202 3363 1184
Size: 1018 1161 4065
Fragments arranged by size:
4065 1161 1018
AlwNI CAG_nnn'CTG
Cuts at: 3987 5559 3987
Size: 1572 4672
ApaLI G'TGCA_C
Cuts at: 4301 5457 4301
Size: 1156 5088
ApoI r'AATT_y
Cuts at: 1493 3041 3553 3779 3931 6040 1493
Size: 1548 512 226 152 2109 1697
Fragments arranged by size:
2109 1697 1548 512 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4798 160
Size: 872 3766 1606
Fragments arranged by size:
3766 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
AvaI C'yCGr_G
Cuts at: 1870 1943 3569 1870
Size: 73 1626 4545
Fragments arranged by size:
4545 1626 73
BamHI G'GATC_C
Cuts at: 3574 3941 3574
Size: 367 5877
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2437 2771 3086 3565 5020
Size: 325 217 627 650 334 315 479 1455
Cuts at: 5020 618
Size: 1842
Fragments arranged by size:
1842 1455 650 627 479 334 325 315
217
BanII G_rGCy'C
Cuts at: 1391 3563 1391
Size: 2172 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3288 928
Size: 971 1389 3884
Fragments arranged by size:
3884 1389 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4435 4469 1903
Size: 34 2498 34 3678
Fragments arranged by size:
3678 2498 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4136 5346 4136
Size: 1210 5034
BclI T'GATC_A
Cuts at: 1548 2799 1548
Size: 1251 4993
BfrBI ATG'CAT
Cuts at: 3146 3146
Size: 6244
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4855 136
Size: 107 122 71 631 605 3183 1525
Fragments arranged by size:
3183 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6239 6239
Size: 6244
BlpI GC'TnA_GC
Cuts at: 3661 3661
Size: 6244
Bme1580I G_kGCm'C
Cuts at: 1790 4305 5461 1790
Size: 2515 1156 2573
Fragments arranged by size:
2573 2515 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6244
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2626 3975 4933
Size: 416 53 83 399 24 1209 1349 958
Cuts at: 4933 442
Size: 1753
Fragments arranged by size:
1753 1349 1209 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3090 4904 1239
Size: 1851 1814 2579
Fragments arranged by size:
2579 1851 1814
Bpu10I CC'TnA_GC
Cuts at: 2400 2400
Size: 6244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2514 4369 5234 5532 2514
Size: 1855 865 298 3226
Fragments arranged by size:
3226 1855 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2860 4907 882
Size: 617 1361 2047 2219
Fragments arranged by size:
2219 2047 1361 617
BsaAI yAC'GTr
Cuts at: 493 1204 4035 493
Size: 711 2831 2702
Fragments arranged by size:
2831 2702 711
BsaBI GATnn'nnATC
Cuts at: 2787 6238 2787
Size: 3451 2793
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4051 4433 5068 275
Size: 53 83 186 936 2518 382 635 1451
Fragments arranged by size:
2518 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2356 4677 5075 5349 5496 5907 2356
Size: 2321 398 274 147 411 2693
Fragments arranged by size:
2693 2321 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6019
Fragments arranged by size:
6019 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3266 1186
Size: 2080 4164
BseYI C'CCAG_C
Cuts at: 5447 5943 5447
Size: 496 5748
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2778 1163
Size: 573 1042 4629
Fragments arranged by size:
4629 1042 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3694 4455 5483 1540
Size: 275 186 1693 761 1028 2301
Fragments arranged by size:
2301 1693 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4305 4390 5461 4305
Size: 85 1071 5088
Fragments arranged by size:
5088 1071 85
BsmI GAATG_Cn'
Cuts at: 3755 3848 3755
Size: 93 6151
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3563 4305 4390 5461 1391
Size: 399 1773 742 85 1071 2174
Fragments arranged by size:
2174 1773 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2356 5907 2356
Size: 3551 2693
BspHI T'CATG_A
Cuts at: 2812 4133 5863 2812
Size: 1321 1730 3193
Fragments arranged by size:
3193 1730 1321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2959 3599 844
Size: 2115 640 3489
Fragments arranged by size:
3489 2115 640
BsrBI CCG'CTC
Cuts at: 1394 1823 4131 1394
Size: 429 2308 3507
Fragments arranged by size:
3507 2308 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3015 3366 4739 4913 65
Size: 1183 1767 351 1373 174 1396
Fragments arranged by size:
1767 1396 1373 1183 351 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4888 5075 1700
Size: 110 3078 187 2869
Fragments arranged by size:
3078 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2484 96
Size: 2388 3856
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6244
BssSI C'ACGA_G
Cuts at: 4304 5316 4304
Size: 1012 5232
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6244
BstBI TT'CG_AA
Cuts at: 5048 5048
Size: 6244
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6244
BstYI r'GATC_y
Cuts at: 1972 2788 3574 3941 4326 4343 5795 5881 5893
Size: 816 786 367 385 17 1452 86 12
Cuts at: 5893 6239 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 816 786 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2889 3073 3171 513
Size: 552 939 885 184 98 3586
Fragments arranged by size:
3586 939 885 552 184 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5505
Fragments arranged by size:
5505 683 56
BtsI GCAGTG_nn'
Cuts at: 2576 3760 4554 4574 2576
Size: 1184 794 20 4246
Fragments arranged by size:
4246 1184 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6209
DraI TTT'AAA
Cuts at: 3548 3548
Size: 6244
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5251 809
Size: 507 3935 1802
Fragments arranged by size:
3935 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2941 3262 3691
Size: 54 1425 50 275 18 1111 321 429
Cuts at: 3691 4580 6209 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1111 889 429 321 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3691 1537
Size: 275 1879 4090
Fragments arranged by size:
4090 1879 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4174 1353
Size: 2821 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4831 5205 5351 387
Size: 1064 138 3242 374 146 1280
Fragments arranged by size:
3242 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2319 3090 4307 4904 5691 5959
Size: 393 408 279 771 1217 597 787 268
Cuts at: 5959 1239
Size: 1524
Fragments arranged by size:
1524 1217 787 771 597 408 393 279
268
EcoRI G'AATT_C
Cuts at: 3553 3931 3553
Size: 378 5866
EcoRV GAT'ATC
Cuts at: 2251 2251
Size: 6244
FspI TGC'GCA
Cuts at: 4750 4750
Size: 6244
HaeII r_GCGC'y
Cuts at: 1678 5391 1678
Size: 3713 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2669 2701 4885 4917 4959
Size: 32 135 32 1097 32 2184 32 42
Cuts at: 4959 4991 1373
Size: 32 2626
Fragments arranged by size:
2626 2184 1097 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3517 3588 3834 669
Size: 988 281 1579 71 246 3079
Fragments arranged by size:
3079 1579 988 281 246 71
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6244
HpaI GTT'AAC
Cuts at: 3834 3834
Size: 6244
KpnI G_GTAC'C
Cuts at: 3090 3569 3090
Size: 479 5765
MfeI C'AATT_G
Cuts at: 3843 3843
Size: 6244
MluI A'CGCG_T
Cuts at: 6120 6120
Size: 6244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2549 5358 5542 1305
Size: 1244 2809 184 2007
Fragments arranged by size:
2809 2007 1244 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2943 3264 10
Size: 54 1425 343 1111 321 2990
Fragments arranged by size:
2990 1425 1111 343 321 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3503 4202 4561 4720 518
Size: 648 2337 699 359 159 2042
Fragments arranged by size:
2337 2042 699 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3419 3660 4337 5485 5730 1606
Size: 1813 241 677 1148 245 2120
Fragments arranged by size:
2120 1813 1148 677 245 241
NcoI C'CATG_G
Cuts at: 513 1065 2004 2889 3073 3171 513
Size: 552 939 885 184 98 3586
Fragments arranged by size:
3586 939 885 552 184 98
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6244
NotI GC'GGCC_GC
Cuts at: 3691 3691
Size: 6244
NsiI A_TGCA'T
Cuts at: 3148 3148
Size: 6244
NspI r_CATG'y
Cuts at: 1132 1229 3241 3289 3350 3602 5147 1132
Size: 97 2012 48 61 252 1545 2229
Fragments arranged by size:
2229 2012 1545 252 97 61 48
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6244
PciI A'CATG_T
Cuts at: 3237 5143 3237
Size: 1906 4338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6244
PmeI GTTT'AAAC
Cuts at: 3548 3548
Size: 6244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 5826 5858 4313
Size: 32 1481 32 4699
Fragments arranged by size:
4699 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6244
PsiI TTA'TAA
Cuts at: 3814 3814
Size: 6244
PstI C_TGCA'G
Cuts at: 830 2583 3064 3217 3596 830
Size: 1753 481 153 379 3478
Fragments arranged by size:
3478 1753 481 379 153
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6244
PvuII CAG'CTG
Cuts at: 3419 3419
Size: 6244
SalI G'TCGA_C
Cuts at: 1936 3586 1936
Size: 1650 4594
SbfI CC_TGCA'GG
Cuts at: 3596 3596
Size: 6244
ScaI AGT'ACT
Cuts at: 4492 6204 4492
Size: 1712 4532
SfcI C'TryA_G
Cuts at: 826 1045 2579 2710 3060 3213 3225 3592 4727
Size: 219 1534 131 350 153 12 367 1135
Cuts at: 4727 5408 5599 826
Size: 681 191 1471
Fragments arranged by size:
1534 1471 1135 681 367 350 219 191
153 131 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6244
SmaI CCC'GGG
Cuts at: 1872 3571 1872
Size: 1699 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2529 2840 4348 5249 5511 820
Size: 197 926 586 311 1508 901 262 1553
Fragments arranged by size:
1553 1508 926 901 586 311 262 197
SnaBI TAC'GTA
Cuts at: 493 4035 493
Size: 3542 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6244
SphI G_CATG'C
Cuts at: 1132 3602 1132
Size: 2470 3774
SspI AAT'ATT
Cuts at: 5 52 2203 2664 3466 4168 5
Size: 47 2151 461 802 702 2081
Fragments arranged by size:
2151 2081 802 702 461 47
StuI AGG'CCT
Cuts at: 1517 3319 1517
Size: 1802 4442
StyI C'CwwG_G
Cuts at: 513 1065 2004 2433 2889 3073 3171 3683 513
Size: 552 939 429 456 184 98 512 3074
Fragments arranged by size:
3074 939 552 512 456 429 184 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2470 4271 4288 4441 4626 5071 325
Size: 1255 890 1801 17 153 185 445 1498
Fragments arranged by size:
1801 1498 1255 890 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2484 2515 3206
Size: 274 80 33 51 1443 507 31 691
Cuts at: 3206 4490 6202 96
Size: 1284 1712 138
Fragments arranged by size:
1712 1443 1284 691 507 274 138 80
51 33 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2260 2292 2371 2403 6204 6236 2260
Size: 32 79 32 3801 32 2268
Fragments arranged by size:
3801 2268 79 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6244
XbaI T'CTAG_A
Cuts at: 3580 3580
Size: 6244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2782 3080 2782
Size: 298 5946
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6244
XmaI C'CCGG_G
Cuts at: 1870 3569 1870
Size: 1699 4545
XmnI GAAnn'nnTTC
Cuts at: 4373 4373
Size: 6244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4052 276
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI