Restriction map for
pFN21AE0965
(Circular) (Six-base) MAPSORT of: pFN21AE0965.seq Check: 7660 from: 1 to: 6232
pFN21AE0965 6232 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2199 3575 1937
Size: 262 1376 4594
Fragments arranged by size:
4594 1376 262
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2790 2873 4295 5679 5947 1632
Size: 1158 83 1422 1384 268 1917
Fragments arranged by size:
1917 1422 1384 1158 268 83
AfeI AGC'GCT
Cuts at: 3143 3143
Size: 6232
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 2103 5131 6108 1203
Size: 900 3028 977 1327
Fragments arranged by size:
3028 1327 977 900
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6232
AlwNI CAG_nnn'CTG
Cuts at: 3975 5547 3975
Size: 1572 4660
ApaLI G'TGCA_C
Cuts at: 4289 5445 4289
Size: 1156 5076
ApoI r'AATT_y
Cuts at: 1493 2359 2447 3541 3767 3919 6028 1493
Size: 866 88 1094 226 152 2109 1697
Fragments arranged by size:
2109 1697 1094 866 226 152 88
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 2481 3557 1870
Size: 73 538 1076 4545
Fragments arranged by size:
4545 1076 538 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2815 2848 2815
Size: 33 6199
BamHI G'GATC_C
Cuts at: 3562 3929 3562
Size: 367 5865
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3134 3553 5008 618
Size: 325 217 627 1347 419 1455 1842
Fragments arranged by size:
1842 1455 1347 627 419 325 217
BanII G_rGCy'C
Cuts at: 1391 2514 3551 1391
Size: 1123 1037 4072
Fragments arranged by size:
4072 1123 1037
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2582 928
Size: 971 683 4578
Fragments arranged by size:
4578 971 683
BbvCI CC'TCA_GC
Cuts at: 2774 2774
Size: 6232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4423 4457 1903
Size: 34 2486 34 3678
Fragments arranged by size:
3678 2486 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2899 4124 5334 2899
Size: 1225 1210 3797
Fragments arranged by size:
3797 1225 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6232
BfrBI ATG'CAT
Cuts at: 3085 3085
Size: 6232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4843 136
Size: 107 122 71 631 605 3171 1525
Fragments arranged by size:
3171 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2709 6227 2709
Size: 3518 2714
BlpI GC'TnA_GC
Cuts at: 3649 3649
Size: 6232
Bme1580I G_kGCm'C
Cuts at: 1790 3137 4293 5449 1790
Size: 1347 1156 1156 2573
Fragments arranged by size:
2573 1347 1156 1156
BmgBI CAC'GTC
Cuts at: 1318 2197 2488 1318
Size: 879 291 5062
Fragments arranged by size:
5062 879 291
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3963 4921 442
Size: 416 53 83 399 24 2546 958 1753
Fragments arranged by size:
2546 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2456 2456
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2812 4892 1239
Size: 1573 2080 2579
Fragments arranged by size:
2579 2080 1573
Bpu10I CC'TnA_GC
Cuts at: 2774 2774
Size: 6232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4357 5222 5520 4357
Size: 865 298 5069
Fragments arranged by size:
5069 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2126 4895 882
Size: 617 627 2769 2219
Fragments arranged by size:
2769 2219 627 617
BsaAI yAC'GTr
Cuts at: 493 1204 4023 493
Size: 711 2819 2702
Fragments arranged by size:
2819 2702 711
BsaBI GATnn'nnATC
Cuts at: 6226 6226
Size: 6232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4039 4421 5056 275
Size: 53 83 186 936 2506 382 635 1451
Fragments arranged by size:
2506 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4665 5063 5337 5484 5895 4665
Size: 398 274 147 411 5002
Fragments arranged by size:
5002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6007
Fragments arranged by size:
6007 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2707 1186
Size: 1521 4711
BseYI C'CCAG_C
Cuts at: 2319 2428 2835 5435 5931 2319
Size: 109 407 2600 496 2620
Fragments arranged by size:
2620 2600 496 407 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5659
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3682 4443 5471 1540
Size: 275 186 1681 761 1028 2301
Fragments arranged by size:
2301 1681 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3098 4293 4378 5449 3098
Size: 1195 85 1071 3881
Fragments arranged by size:
3881 1195 1071 85
BsmI GAATG_Cn'
Cuts at: 3085 3437 3743 3836 3085
Size: 352 306 93 5481
Fragments arranged by size:
5481 352 306 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2478 2478
Size: 6232
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2514 3098 3137 3551 4293 4378 5449
Size: 399 724 584 39 414 742 85 1071
Cuts at: 5449 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 724 584 414 399 85
39
BspEI T'CCGG_A
Cuts at: 5895 5895
Size: 6232
BspHI T'CATG_A
Cuts at: 2040 2860 4121 5851 2040
Size: 820 1261 1730 2421
Fragments arranged by size:
2421 1730 1261 820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2646 3587 844
Size: 1802 941 3489
Fragments arranged by size:
3489 1802 941
BsrBI CCG'CTC
Cuts at: 1394 1823 4119 1394
Size: 429 2296 3507
Fragments arranged by size:
3507 2296 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2799 2997 3271 4727 4901 65
Size: 1183 1551 198 274 1456 174 1396
Fragments arranged by size:
1551 1456 1396 1183 274 198 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4876 5063 1700
Size: 110 3066 187 2869
Fragments arranged by size:
3066 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2567 2757 96
Size: 2471 190 3571
Fragments arranged by size:
3571 2471 190
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6232
BstBI TT'CG_AA
Cuts at: 2923 5036 2923
Size: 2113 4119
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6232
BstXI CCAn_nnnn'nTGG
Cuts at: 3181 3256 3181
Size: 75 6157
BstYI r'GATC_y
Cuts at: 1972 2709 3562 3929 4314 4331 5783 5869 5881
Size: 737 853 367 385 17 1452 86 12
Cuts at: 5881 6227 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 853 737 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2274 513
Size: 552 939 270 4471
Fragments arranged by size:
4471 939 552 270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5493
Fragments arranged by size:
5493 683 56
BtsI GCAGTG_nn'
Cuts at: 2165 3467 3748 4542 4562 2165
Size: 1302 281 794 20 3835
Fragments arranged by size:
3835 1302 794 281 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2509 2544 529
Size: 35 1945 35 4217
Fragments arranged by size:
4217 1945 35 35
DraI TTT'AAA
Cuts at: 2452 3015 3536 2452
Size: 563 521 5148
Fragments arranged by size:
5148 563 521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2257 5239 809
Size: 507 941 2982 1802
Fragments arranged by size:
2982 1802 941 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2266 3246 3414
Size: 54 1425 50 275 18 436 980 168
Cuts at: 3414 3679 4568 6197 8
Size: 265 889 1629 43
Fragments arranged by size:
1629 1425 980 889 436 275 265 168
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4162 1353
Size: 2809 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3268 4819 5193 5339 387
Size: 1064 138 1679 1551 374 146 1280
Fragments arranged by size:
1679 1551 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2790 2812 2873 4295 4892 5679 5947
Size: 393 1158 22 61 1422 597 787 268
Cuts at: 5947 1239
Size: 1524
Fragments arranged by size:
1524 1422 1158 787 597 393 268 61
22
EcoO109I rG'GnC_Cy
Cuts at: 2979 2979
Size: 6232
EcoRI G'AATT_C
Cuts at: 3541 3919 3541
Size: 378 5854
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 3145 5379 1678
Size: 1467 2234 2531
Fragments arranged by size:
2531 2234 1467
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2717 2749 2801 2833 3085
Size: 32 135 32 1145 32 52 32 252
Cuts at: 3085 3117 4873 4905 4947 4979 1373
Size: 32 1756 32 42 32 2626
Fragments arranged by size:
2626 1756 1145 252 135 52 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2089 3576 3822 669
Size: 988 281 151 1487 246 3079
Fragments arranged by size:
3079 1487 988 281 246 151
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6232
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2509 2616 5346 5530 1305
Size: 1204 107 2730 184 2007
Fragments arranged by size:
2730 2007 1204 184 107
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2268 3248 3416 10
Size: 54 1425 343 436 980 168 2826
Fragments arranged by size:
2826 1425 980 436 343 168 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2768 2859 3179 4190 4549 4708
Size: 648 942 660 91 320 1011 359 159
Cuts at: 4708 518
Size: 2042
Fragments arranged by size:
2042 1011 942 660 648 359 320 159
91
MspA1I CmG'CkG
Cuts at: 1606 2323 2682 2839 3648 4325 5473 5718 1606
Size: 717 359 157 809 677 1148 245 2120
Fragments arranged by size:
2120 1148 809 717 677 359 245 157
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4741
Fragments arranged by size:
4741 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6232
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NsiI A_TGCA'T
Cuts at: 3087 3087
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 2107 3590 5135 1132
Size: 97 878 1483 1545 2229
Fragments arranged by size:
2229 1545 1483 878 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6232
PciI A'CATG_T
Cuts at: 2103 5131 2103
Size: 3028 3204
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2275 3384 1108
Size: 1167 1109 3956
Fragments arranged by size:
3956 1167 1109
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2634 4301 4333 5814 5846 2602
Size: 32 1667 32 1481 32 2988
Fragments arranged by size:
2988 1667 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2979 2979
Size: 6232
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6232
PsiI TTA'TAA
Cuts at: 3201 3802 3201
Size: 601 5631
PstI C_TGCA'G
Cuts at: 830 3584 830
Size: 2754 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
PvuII CAG'CTG
Cuts at: 2323 2682 2839 2323
Size: 359 157 5716
Fragments arranged by size:
5716 359 157
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SanDI GG'GwC_CC
Cuts at: 2979 2979
Size: 6232
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 4480 6192 4480
Size: 1712 4520
SfcI C'TryA_G
Cuts at: 826 1045 2394 3580 4715 5396 5587 826
Size: 219 1349 1186 1135 681 191 1471
Fragments arranged by size:
1471 1349 1186 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 3559 1872
Size: 1687 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4336 5237 5499 820
Size: 197 926 2393 901 262 1553
Fragments arranged by size:
2393 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3590 1132
Size: 2458 3774
SspI AAT'ATT
Cuts at: 5 52 3028 4156 5
Size: 47 2976 1128 2081
Fragments arranged by size:
2976 2081 1128 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6232
StyI C'CwwG_G
Cuts at: 513 1065 2004 2082 2287 2983 3671 513
Size: 552 939 78 205 696 688 3074
Fragments arranged by size:
3074 939 696 688 552 205 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4259 4276 4429 4614 5059 325
Size: 1255 2679 17 153 185 445 1498
Fragments arranged by size:
2679 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2567 2757 4478
Size: 274 80 33 51 1443 590 190 1721
Cuts at: 4478 6190 96
Size: 1712 138
Fragments arranged by size:
1721 1712 1443 590 274 190 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2346 2378 2549 2581 6192 6224 2346
Size: 32 171 32 3611 32 2354
Fragments arranged by size:
3611 2354 171 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6232
XbaI T'CTAG_A
Cuts at: 3163 3568 3163
Size: 405 5827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2372 3145 2372
Size: 773 5459
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 3557 1870
Size: 1687 4545
XmnI GAAnn'nnTTC
Cuts at: 2711 4361 2711
Size: 1650 4582
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI