Restriction map for
pFN21AE0961
(Circular) (Six-base) MAPSORT of: pFN21AE0961.seq Check: 9204 from: 1 to: 6208
pFN21AE0961 6208 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2165 3047 2165
Size: 882 5326
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2495 4271 5655 5923 1632
Size: 863 1776 1384 268 1917
Fragments arranged by size:
1917 1776 1384 863 268
AfeI AGC'GCT
Cuts at: 3280 3280
Size: 6208
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 5107 6084 1203
Size: 3904 977 1327
Fragments arranged by size:
3904 1327 977
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 4937 4937
Size: 6208
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3371 1184
Size: 2187 4021
AlwNI CAG_nnn'CTG
Cuts at: 3038 3101 3197 3245 3269 3293 3951 5523 3038
Size: 63 96 48 24 24 658 1572 3723
Fragments arranged by size:
3723 1572 658 96 63 48 24 24
ApaLI G'TGCA_C
Cuts at: 2429 4265 5421 2429
Size: 1836 1156 3216
Fragments arranged by size:
3216 1836 1156
ApoI r'AATT_y
Cuts at: 1493 3517 3743 3895 6004 1493
Size: 2024 226 152 2109 1697
Fragments arranged by size:
2109 2024 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2555 3047 3533 1870
Size: 73 612 492 486 4545
Fragments arranged by size:
4545 612 492 486 73
BamHI G'GATC_C
Cuts at: 2334 3538 3905 2334
Size: 1204 367 4637
Fragments arranged by size:
4637 1204 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 3425 3529 4984 618
Size: 325 217 627 1199 439 104 1455 1842
Fragments arranged by size:
1842 1455 1199 627 439 325 217 104
BanII G_rGCy'C
Cuts at: 1391 2544 3527 1391
Size: 1153 983 4072
Fragments arranged by size:
4072 1153 983
BbeI G_GCGC'C
Cuts at: 3429 3429
Size: 6208
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5237
BbvCI CC'TCA_GC
Cuts at: 3025 3094 3025
Size: 69 6139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3000 3034 4399 4433 1903
Size: 34 50 34 979 34 1365 34 3678
Fragments arranged by size:
3678 1365 979 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4100 5310 4100
Size: 1210 4998
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3020 3359 3368
Size: 107 122 71 631 605 1348 339 9
Cuts at: 3368 4819 136
Size: 1451 1525
Fragments arranged by size:
1525 1451 1348 631 605 339 122 107
71 9
BglII A'GATC_T
Cuts at: 6203 6203
Size: 6208
BlpI GC'TnA_GC
Cuts at: 2514 3625 2514
Size: 1111 5097
Bme1580I G_kGCm'C
Cuts at: 1790 2433 2911 2989 3336 3481 4269 5425 1790
Size: 643 478 78 347 145 788 1156 2573
Fragments arranged by size:
2573 1156 788 643 478 347 145 78
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6208
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3010 3939 4897
Size: 416 53 83 399 24 1593 929 958
Cuts at: 4897 442
Size: 1753
Fragments arranged by size:
1753 1593 958 929 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2764 2863 3300 2764
Size: 99 437 5672
Fragments arranged by size:
5672 437 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2321 2414 2468 3222 3270 4868 1239
Size: 1082 93 54 754 48 1598 2579
Fragments arranged by size:
2579 1598 1082 754 93 54 48
Bpu10I CC'TnA_GC
Cuts at: 3025 3094 3384 3025
Size: 69 290 5849
Fragments arranged by size:
5849 290 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2405 2426 4333 5198 5496 2210
Size: 195 21 1907 865 298 2922
Fragments arranged by size:
2922 1907 865 298 195 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4871 882
Size: 617 3372 2219
Fragments arranged by size:
3372 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3999 493
Size: 711 2795 2702
Fragments arranged by size:
2795 2702 711
BsaBI GATnn'nnATC
Cuts at: 2219 2492 2706 6202 2219
Size: 273 214 3496 2225
Fragments arranged by size:
3496 2225 273 214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2599 3426 4015 4397
Size: 53 83 186 936 1066 827 589 382
Cuts at: 4397 5032 275
Size: 635 1451
Fragments arranged by size:
1451 1066 936 827 635 589 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4641 5039 5313 5460 5871 4641
Size: 398 274 147 411 4978
Fragments arranged by size:
4978 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5983
Fragments arranged by size:
5983 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2684 3512 1186
Size: 931 567 828 3882
Fragments arranged by size:
3882 931 828 567
BseYI C'CCAG_C
Cuts at: 2613 3166 3190 3214 3262 3399 5411 5907 2613
Size: 553 24 24 48 137 2012 496 2914
Fragments arranged by size:
2914 2012 553 496 137 48 24 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 1163
Size: 573 399 5236
Fragments arranged by size:
5236 573 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2185 3658 4419 5447 1540
Size: 275 186 184 1473 761 1028 2301
Fragments arranged by size:
2301 1473 1028 761 275 186 184
BsiHKAI G_wGCw'C
Cuts at: 2433 3329 4269 4354 5425 2433
Size: 896 940 85 1071 3216
Fragments arranged by size:
3216 1071 940 896 85
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2433 2544 2911 2989 3329 3336 3481
Size: 399 643 111 367 78 340 7 145
Cuts at: 3481 3527 4269 4354 5425 1391
Size: 46 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 643 399 367 340 145
111 85 78 46 7
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 2970 4097 5827 2970
Size: 1127 1730 3351
Fragments arranged by size:
3351 1730 1127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2165 3047 3563 844
Size: 1321 882 516 3489
Fragments arranged by size:
3489 1321 882 516
BsrBI CCG'CTC
Cuts at: 1394 1823 4095 1394
Size: 429 2272 3507
Fragments arranged by size:
3507 2272 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2875 4703 4877 65
Size: 1183 1627 1828 174 1396
Fragments arranged by size:
1828 1627 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2469 2583 3388 4852 5039 1700
Size: 110 659 114 805 1464 187 2869
Fragments arranged by size:
2869 1464 805 659 187 114 110
BsrGI T'GTAC_A
Cuts at: 96 2634 96
Size: 2538 3670
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6208
BssSI C'ACGA_G
Cuts at: 3117 4268 5280 3117
Size: 1151 1012 4045
Fragments arranged by size:
4045 1151 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6208
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6208
BstXI CCAn_nnnn'nTGG
Cuts at: 2788 2788
Size: 6208
BstYI r'GATC_y
Cuts at: 1972 2334 2721 3538 3905 4290 4307 5759 5845
Size: 362 387 817 367 385 17 1452 86
Cuts at: 5845 5857 6203 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 817 387 385 367 362 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3102 3102
Size: 6208
BtgI C'CryG_G
Cuts at: 513 1065 2310 2400 513
Size: 552 1245 90 4321
Fragments arranged by size:
4321 1245 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5469
Fragments arranged by size:
5469 683 56
BtsI GCAGTG_nn'
Cuts at: 2829 3036 3211 3355 3724 4518 4538 2829
Size: 207 175 144 369 794 20 4499
Fragments arranged by size:
4499 794 369 207 175 144 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2358 2393 529
Size: 35 1794 35 4344
Fragments arranged by size:
4344 1794 35 35
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5215 809
Size: 507 3899 1802
Fragments arranged by size:
3899 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2182 2307 3360
Size: 54 1425 50 275 18 352 125 1053
Cuts at: 3360 3655 4544 6173 8
Size: 295 889 1629 43
Fragments arranged by size:
1629 1425 1053 889 352 295 275 125
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2182 3655 1537
Size: 275 370 1473 4090
Fragments arranged by size:
4090 1473 370 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2717 2832 3315 3456 4138 1353
Size: 1364 115 483 141 682 3423
Fragments arranged by size:
3423 1364 682 483 141 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4795 5169 5315 387
Size: 1064 138 3206 374 146 1280
Fragments arranged by size:
3206 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2321 2414 2468 2495 3222 3270 4271
Size: 393 689 93 54 27 727 48 1001
Cuts at: 4271 4868 5655 5923 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1001 787 727 689 597 393 268
93 54 48 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2342 2534 2342
Size: 192 6016
EcoO109I rG'GnC_Cy
Cuts at: 2550 2550
Size: 6208
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2305 2728 2305
Size: 423 5785
FspI TGC'GCA
Cuts at: 4714 4714
Size: 6208
HaeII r_GCGC'y
Cuts at: 1678 3282 3429 5355 1678
Size: 1604 147 1926 2531
Fragments arranged by size:
2531 1926 1604 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2076 2108 4849 4881 4923
Size: 32 135 32 504 32 2741 32 42
Cuts at: 4923 4955 1373
Size: 32 2626
Fragments arranged by size:
2741 2626 504 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3552 3798 669
Size: 988 281 1614 246 3079
Fragments arranged by size:
3079 1614 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KasI G'GCGC_C
Cuts at: 3425 3425
Size: 6208
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5322 5506 1305
Size: 4017 184 2007
Fragments arranged by size:
4017 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2309 3362 10
Size: 54 1425 343 477 1053 2856
Fragments arranged by size:
2856 1425 1053 477 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2969 4166 4525 4684 518
Size: 648 1803 1197 359 159 2042
Fragments arranged by size:
2042 1803 1197 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2257 2590 2824 3071 3077 3374 3624 4301
Size: 651 333 234 247 6 297 250 677
Cuts at: 4301 5449 5694 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 651 333 297 250 247
245 234 6
NaeI GCC'GGC
Cuts at: 2585 3390 2585
Size: 805 5403
NarI GG'CG_CC
Cuts at: 3426 3426
Size: 6208
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5656
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NgoMIV G'CCGG_C
Cuts at: 2583 3388 2583
Size: 805 5403
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 3566 5111 1132
Size: 97 2337 1545 2229
Fragments arranged by size:
2337 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6208
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6208
PfoI T'CCnGG_A
Cuts at: 2298 2337 2391 2298
Size: 39 54 6115
Fragments arranged by size:
6115 54 39
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 5790 5822 4277
Size: 32 1481 32 4663
Fragments arranged by size:
4663 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2550 2550
Size: 6208
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2444 2476 2444
Size: 32 6176
PstI C_TGCA'G
Cuts at: 830 2836 3013 3058 3076 3560 830
Size: 2006 177 45 18 484 3478
Fragments arranged by size:
3478 2006 484 177 45 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 2257 3071 3077 3374 2257
Size: 814 6 297 5091
Fragments arranged by size:
5091 814 297 6
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SanDI GG'GwC_CC
Cuts at: 2550 2550
Size: 6208
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 2288 4456 6168 2288
Size: 2168 1712 2328
Fragments arranged by size:
2328 2168 1712
SexAI A'CCwGG_T
Cuts at: 2454 2787 2454
Size: 333 5875
SfcI C'TryA_G
Cuts at: 826 1045 2252 2832 3009 3054 3072 3556 4691
Size: 219 1207 580 177 45 18 484 1135
Cuts at: 4691 5372 5563 826
Size: 681 191 1471
Fragments arranged by size:
1471 1207 1135 681 580 484 219 191
177 45 18
SfoI GGC'GCC
Cuts at: 3427 3427
Size: 6208
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 3049 3535 1872
Size: 1177 486 4545
Fragments arranged by size:
4545 1177 486
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2225 2420 2441 4312 5213 5475
Size: 197 926 282 195 21 1871 901 262
Cuts at: 5475 820
Size: 1553
Fragments arranged by size:
1871 1553 926 901 282 262 197 195
21
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 3566 1132
Size: 2434 3774
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6208
StyI C'CwwG_G
Cuts at: 513 1065 2127 3366 3647 513
Size: 552 1062 1239 281 3074
Fragments arranged by size:
3074 1239 1062 552 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2004 3356 4235 4252 4405 4590 5035
Size: 1255 424 1352 879 17 153 185 445
Cuts at: 5035 325
Size: 1498
Fragments arranged by size:
1498 1352 1255 879 445 424 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2286 2634 4454
Size: 274 80 33 51 1443 309 348 1820
Cuts at: 4454 6166 96
Size: 1712 138
Fragments arranged by size:
1820 1712 1443 348 309 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2589 2621 6168 6200 2589
Size: 32 3547 32 2597
Fragments arranged by size:
3547 2597 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2789 1316
Size: 1473 4735
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6208
XmaI C'CCGG_G
Cuts at: 1870 3047 3533 1870
Size: 1177 486 4545
Fragments arranged by size:
4545 1177 486
XmnI GAAnn'nnTTC
Cuts at: 2711 4337 2711
Size: 1626 4582
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI RsrII SacI SacII SapI
SfiI SrfI SwaI XcmI