Restriction map for
pFN21AE0960
(Circular) (Six-base) MAPSORT of: pFN21AE0960.seq Check: 8980 from: 1 to: 6202
pFN21AE0960 6202 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4012 278
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2982 3545 1937
Size: 1045 563 4594
Fragments arranged by size:
4594 1045 563
Acc65I G'GTAC_C
Cuts at: 2022 3523 2022
Size: 1501 4701
AclI AA'CG_TT
Cuts at: 1563 4329 4702 1563
Size: 2766 373 3063
Fragments arranged by size:
3063 2766 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 3410 4265 5649 5917 1632
Size: 968 810 855 1384 268 1917
Fragments arranged by size:
1917 1384 968 855 810 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6005
AflIII A'CryG_T
Cuts at: 1203 2718 5101 6078 1203
Size: 1515 2383 977 1327
Fragments arranged by size:
2383 1515 1327 977
AgeI A'CCGG_T
Cuts at: 5033 5033
Size: 6202
AhdI GACnn_n'nnGTC
Cuts at: 4931 4931
Size: 6202
AleI CACnn'nnGTG
Cuts at: 1166 2969 1166
Size: 1803 4399
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2987 3332 1184
Size: 1803 345 4054
Fragments arranged by size:
4054 1803 345
AlwNI CAG_nnn'CTG
Cuts at: 3945 5517 3945
Size: 1572 4630
ApaLI G'TGCA_C
Cuts at: 4259 5415 4259
Size: 1156 5046
ApoI r'AATT_y
Cuts at: 1493 2902 3511 3737 3889 5998 1493
Size: 1409 609 226 152 2109 1697
Fragments arranged by size:
2109 1697 1409 609 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4756 160
Size: 872 3724 1606
Fragments arranged by size:
3724 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
AvaI C'yCGr_G
Cuts at: 1870 1943 2568 3527 1870
Size: 73 625 959 4545
Fragments arranged by size:
4545 959 625 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2707 2740 2707
Size: 33 6169
BamHI G'GATC_C
Cuts at: 3532 3899 3532
Size: 367 5835
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2022 3523 4978 618
Size: 325 217 627 235 1501 1455 1842
Fragments arranged by size:
1842 1501 1455 627 325 235 217
BanII G_rGCy'C
Cuts at: 1391 2569 2919 3521 1391
Size: 1178 350 602 4072
Fragments arranged by size:
4072 1178 602 350
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5231
BbvCI CC'TCA_GC
Cuts at: 2445 2445
Size: 6202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4393 4427 1903
Size: 34 50 34 2372 34 3678
Fragments arranged by size:
3678 2372 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4094 5304 4094
Size: 1210 4992
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6202
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4813 136
Size: 107 122 71 631 605 3141 1525
Fragments arranged by size:
3141 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6197 6197
Size: 6202
BlpI GC'TnA_GC
Cuts at: 3399 3456 3619 3399
Size: 57 163 5982
Fragments arranged by size:
5982 163 57
Bme1580I G_kGCm'C
Cuts at: 1790 4263 5419 1790
Size: 2473 1156 2573
Fragments arranged by size:
2573 2473 1156
BmgBI CAC'GTC
Cuts at: 1318 2467 1318
Size: 1149 5053
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3933 4891 442
Size: 416 53 83 399 24 2516 958 1753
Fragments arranged by size:
2516 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3466 4862 1239
Size: 2227 1396 2579
Fragments arranged by size:
2579 2227 1396
Bpu10I CC'TnA_GC
Cuts at: 2445 2747 2445
Size: 302 5900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2645 2861 3086 4327 5192 5490 2645
Size: 216 225 1241 865 298 3357
Fragments arranged by size:
3357 1241 865 298 225 216
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4865 882
Size: 617 3366 2219
Fragments arranged by size:
3366 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3993 493
Size: 711 2789 2702
Fragments arranged by size:
2789 2702 711
BsaBI GATnn'nnATC
Cuts at: 6196 6196
Size: 6202
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2137 4009 4391 5026
Size: 53 83 186 936 604 1872 382 635
Cuts at: 5026 275
Size: 1451
Fragments arranged by size:
1872 1451 936 635 604 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2124 2199 4635 5033 5307 5454 5865 2124
Size: 75 2436 398 274 147 411 2461
Fragments arranged by size:
2461 2436 411 398 274 147 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2044 2074 1178
Size: 30 165 30 641 30 5306
Fragments arranged by size:
5306 641 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2020 2383 1186
Size: 834 363 5005
Fragments arranged by size:
5005 834 363
BseYI C'CCAG_C
Cuts at: 3284 5405 5901 3284
Size: 2121 496 3585
Fragments arranged by size:
3585 2121 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5629
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2200 2530 3652 4413 5441 1540
Size: 275 186 199 330 1122 761 1028 2301
Fragments arranged by size:
2301 1122 1028 761 330 275 199 186
BsiHKAI G_wGCw'C
Cuts at: 2919 2966 3015 3052 4263 4348 5419 2919
Size: 47 49 37 1211 85 1071 3702
Fragments arranged by size:
3702 1211 1071 85 49 47 37
BsmI GAATG_Cn'
Cuts at: 2293 3713 3806 2293
Size: 1420 93 4689
Fragments arranged by size:
4689 1420 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2569 2919 2966 3015 3052 3521 4263
Size: 399 779 350 47 49 37 469 742
Cuts at: 4263 4348 5419 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 779 742 469 399 350 85
49 47 37
BspEI T'CCGG_A
Cuts at: 5865 5865
Size: 6202
BspHI T'CATG_A
Cuts at: 4091 5821 4091
Size: 1730 4472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2790 3557 844
Size: 1946 767 3489
Fragments arranged by size:
3489 1946 767
BsrBI CCG'CTC
Cuts at: 1394 1823 2394 4089 1394
Size: 429 571 1695 3507
Fragments arranged by size:
3507 1695 571 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4697 4871 65
Size: 1183 3449 174 1396
Fragments arranged by size:
3449 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2214 4846 5033 1700
Size: 110 404 2632 187 2869
Fragments arranged by size:
2869 2632 404 187 110
BsrGI T'GTAC_A
Cuts at: 96 2536 96
Size: 2440 3762
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6202
BssSI C'ACGA_G
Cuts at: 4262 5274 4262
Size: 1012 5190
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6202
BstBI TT'CG_AA
Cuts at: 5006 5006
Size: 6202
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6202
BstYI r'GATC_y
Cuts at: 1972 3189 3532 3899 4284 4301 5753 5839 5851
Size: 1217 343 367 385 17 1452 86 12
Cuts at: 5851 6197 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1217 385 367 346 343 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2509 2509
Size: 6202
BtgI C'CryG_G
Cuts at: 513 1065 2710 513
Size: 552 1645 4005
Fragments arranged by size:
4005 1645 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2537 487
Size: 683 56 1311 4152
Fragments arranged by size:
4152 1311 683 56
BtsI GCAGTG_nn'
Cuts at: 2959 2998 3718 4512 4532 2959
Size: 39 720 794 20 4629
Fragments arranged by size:
4629 794 720 39 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6167
DraI TTT'AAA
Cuts at: 3506 3506
Size: 6202
DraIII CAC_nnn'GTG
Cuts at: 2803 2803
Size: 6202
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2097 5209 809
Size: 507 781 3112 1802
Fragments arranged by size:
3112 1802 781 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2527 2713 3649
Size: 54 1425 50 275 18 697 186 936
Cuts at: 3649 4538 6167 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 936 889 697 275 186 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2527 3649 1537
Size: 275 715 1122 4090
Fragments arranged by size:
4090 1122 715 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2954 3149 3365 4132 1353
Size: 1601 195 216 767 3423
Fragments arranged by size:
3423 1601 767 216 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2380 4789 5163 5309 387
Size: 1064 138 791 2409 374 146 1280
Fragments arranged by size:
2409 1280 1064 791 374 146 138
EcoICRI GAG'CTC
Cuts at: 2917 2917
Size: 6202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2600 3410 3466 4265 4862 5649 5917
Size: 393 968 810 56 799 597 787 268
Cuts at: 5917 1239
Size: 1524
Fragments arranged by size:
1524 968 810 799 787 597 393 268
56
EcoO109I rG'GnC_Cy
Cuts at: 2520 3197 2520
Size: 677 5525
EcoRI G'AATT_C
Cuts at: 2902 3511 3889 2902
Size: 609 378 5215
Fragments arranged by size:
5215 609 378
EcoRV GAT'ATC
Cuts at: 3493 3493
Size: 6202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3080 3080
Size: 6202
FspI TGC'GCA
Cuts at: 4708 4708
Size: 6202
HaeII r_GCGC'y
Cuts at: 1678 3292 5349 1678
Size: 1614 2057 2531
Fragments arranged by size:
2531 2057 1614
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 2481 2513 2613
Size: 32 135 32 605 32 272 32 100
Cuts at: 2613 2645 3126 3158 3181 3213 3263 3295 4843
Size: 32 481 32 23 32 50 32 1548
Cuts at: 4843 4875 4917 4949 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1548 605 481 272 135 100 50
42 32 32 32 32 32 32 32
32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 2548 3546 3792 669
Size: 988 281 610 998 246 3079
Fragments arranged by size:
3079 998 988 610 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6202
HpaI GTT'AAC
Cuts at: 3792 3792
Size: 6202
KpnI G_GTAC'C
Cuts at: 2026 3527 2026
Size: 1501 4701
MfeI C'AATT_G
Cuts at: 3801 3801
Size: 6202
MluI A'CGCG_T
Cuts at: 6078 6078
Size: 6202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2220 5316 5500 1305
Size: 915 3096 184 2007
Fragments arranged by size:
3096 2007 915 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2715 10
Size: 54 1425 343 883 3497
Fragments arranged by size:
3497 1425 883 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 2969 4160 4519 4678 518
Size: 648 1132 671 1191 359 159 2042
Fragments arranged by size:
2042 1191 1132 671 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2311 2824 2969 3284 3316 3618 4295 5443
Size: 705 513 145 315 32 302 677 1148
Cuts at: 5443 5688 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 705 677 513 315 302 245
145 32
NcoI C'CATG_G
Cuts at: 513 1065 2710 513
Size: 552 1645 4005
Fragments arranged by size:
4005 1645 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6202
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6202
NotI GC'GGCC_GC
Cuts at: 3649 3649
Size: 6202
NspI r_CATG'y
Cuts at: 1132 1229 2722 3382 3560 5105 1132
Size: 97 1493 660 178 1545 2229
Fragments arranged by size:
2229 1545 1493 660 178 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6202
PciI A'CATG_T
Cuts at: 2718 5101 2718
Size: 2383 3819
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6202
PfoI T'CCnGG_A
Cuts at: 3209 3209
Size: 6202
PmeI GTTT'AAAC
Cuts at: 3506 3506
Size: 6202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4271 4303 5784 5816 4271
Size: 32 1481 32 4657
Fragments arranged by size:
4657 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2520 3197 2520
Size: 677 5525
PshAI GACnn'nnGTC
Cuts at: 1613 3483 1613
Size: 1870 4332
PsiI TTA'TAA
Cuts at: 3772 3772
Size: 6202
PspXI vC'TCGA_Gb
Cuts at: 2568 2568
Size: 6202
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2309 2341 2309
Size: 32 6170
PstI C_TGCA'G
Cuts at: 830 3554 830
Size: 2724 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6202
PvuII CAG'CTG
Cuts at: 2824 2969 3284 2824
Size: 145 315 5742
Fragments arranged by size:
5742 315 145
SacI G_AGCT'C
Cuts at: 2919 2919
Size: 6202
SalI G'TCGA_C
Cuts at: 1936 3544 1936
Size: 1608 4594
SapI GCTCTTCn'nnn_
Cuts at: 2954 3365 2954
Size: 411 5791
SbfI CC_TGCA'GG
Cuts at: 3554 3554
Size: 6202
ScaI AGT'ACT
Cuts at: 4450 6162 4450
Size: 1712 4490
SfcI C'TryA_G
Cuts at: 826 1045 2233 3550 4685 5366 5557 826
Size: 219 1188 1317 1135 681 191 1471
Fragments arranged by size:
1471 1317 1188 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6202
SmaI CCC'GGG
Cuts at: 1872 3529 1872
Size: 1657 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2568 2624 2876 3065 4306 5207
Size: 197 926 625 56 252 189 1241 901
Cuts at: 5207 5469 820
Size: 262 1553
Fragments arranged by size:
1553 1241 926 901 625 262 252 197
189 56
SnaBI TAC'GTA
Cuts at: 493 3993 493
Size: 3500 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6202
SphI G_CATG'C
Cuts at: 1132 3382 3560 1132
Size: 2250 178 3774
Fragments arranged by size:
3774 2250 178
SspI AAT'ATT
Cuts at: 5 52 2890 3242 4126 5
Size: 47 2838 352 884 2081
Fragments arranged by size:
2838 2081 884 352 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6202
StyI C'CwwG_G
Cuts at: 513 1065 2710 3411 3641 513
Size: 552 1645 701 230 3074
Fragments arranged by size:
3074 1645 701 552 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3404 4229 4246 4399 4584 5029 325
Size: 1255 1824 825 17 153 185 445 1498
Fragments arranged by size:
1824 1498 1255 825 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2536 2604 4448
Size: 274 80 33 51 1443 559 68 1844
Cuts at: 4448 6160 96
Size: 1712 138
Fragments arranged by size:
1844 1712 1443 559 274 138 80 68
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2044 2076 6162 6194 2044
Size: 32 4086 32 2052
Fragments arranged by size:
4086 2052 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2136 1316
Size: 820 5382
XbaI T'CTAG_A
Cuts at: 3538 3538
Size: 6202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2558 2558
Size: 6202
XhoI C'TCGA_G
Cuts at: 1943 2568 1943
Size: 625 5577
XmaI C'CCGG_G
Cuts at: 1870 3527 1870
Size: 1657 4545
XmnI GAAnn'nnTTC
Cuts at: 2889 3423 4331 2889
Size: 534 908 4760
Fragments arranged by size:
4760 908 534
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4010 276
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI