Restriction map for
pFN21AE0959
(Circular) (Six-base) MAPSORT of: pFN21AE0959.seq Check: 3101 from: 1 to: 6202
pFN21AE0959 6202 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3369 3369
Size: 6202
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4012 278
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3545 1937
Size: 1608 4594
Acc65I G'GTAC_C
Cuts at: 3523 3523
Size: 6202
AclI AA'CG_TT
Cuts at: 1563 4329 4702 1563
Size: 2766 373 3063
Fragments arranged by size:
3063 2766 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4265 5649 5917 1632
Size: 2633 1384 268 1917
Fragments arranged by size:
2633 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6005
AflIII A'CryG_T
Cuts at: 1203 5101 6078 1203
Size: 3898 977 1327
Fragments arranged by size:
3898 1327 977
AgeI A'CCGG_T
Cuts at: 5033 5033
Size: 6202
AhdI GACnn_n'nnGTC
Cuts at: 4931 4931
Size: 6202
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6202
AlwNI CAG_nnn'CTG
Cuts at: 2114 2689 2792 2947 3160 3223 3294 3945 5517
Size: 575 103 155 213 63 71 651 1572
Cuts at: 5517 2114
Size: 2799
Fragments arranged by size:
2799 1572 651 575 213 155 103 71
63
ApaI G_GGCC'C
Cuts at: 2447 2461 2580 2733 3148 3230 2447
Size: 14 119 153 415 82 5419
Fragments arranged by size:
5419 415 153 119 82 14
ApaLI G'TGCA_C
Cuts at: 2693 3382 4259 5415 2693
Size: 689 877 1156 3480
Fragments arranged by size:
3480 1156 877 689
ApoI r'AATT_y
Cuts at: 1493 3511 3737 3889 5998 1493
Size: 2018 226 152 2109 1697
Fragments arranged by size:
2109 2018 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4756 160
Size: 872 3724 1606
Fragments arranged by size:
3724 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
AvaI C'yCGr_G
Cuts at: 1870 1943 2733 2831 3432 3527 1870
Size: 73 790 98 601 95 4545
Fragments arranged by size:
4545 790 601 98 95 73
AvrII C'CTAG_G
Cuts at: 3310 3310
Size: 6202
BamHI G'GATC_C
Cuts at: 3532 3899 3532
Size: 367 5835
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2072 2226 2609 3523
Size: 325 217 627 264 21 154 383 914
Cuts at: 3523 4978 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 914 627 383 325 264 217
154 21
BanII G_rGCy'C
Cuts at: 1391 2145 2288 2447 2461 2580 2733 2849 3148
Size: 754 143 159 14 119 153 116 299
Cuts at: 3148 3230 3440 3521 1391
Size: 82 210 81 4072
Fragments arranged by size:
4072 754 299 210 159 153 143 119
116 82 81 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2368 2982 928
Size: 971 469 614 4148
Fragments arranged by size:
4148 971 614 469
BbvCI CC'TCA_GC
Cuts at: 3470 3470
Size: 6202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4393 4427 1903
Size: 34 50 34 2372 34 3678
Fragments arranged by size:
3678 2372 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4094 5304 4094
Size: 1210 4992
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6202
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2140 2352 3065
Size: 107 122 71 631 605 468 212 713
Cuts at: 3065 4813 136
Size: 1748 1525
Fragments arranged by size:
1748 1525 713 631 605 468 212 122
107 71
BglII A'GATC_T
Cuts at: 6197 6197
Size: 6202
BlpI GC'TnA_GC
Cuts at: 2144 2342 3619 2144
Size: 198 1277 4727
Fragments arranged by size:
4727 1277 198
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2447 2461 2580 2697 2733 3148 3230
Size: 264 393 14 119 117 36 415 82
Cuts at: 3230 3386 4263 5419 1790
Size: 156 877 1156 2573
Fragments arranged by size:
2573 1156 877 415 393 264 156 119
117 82 36 14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2078 3304 3933
Size: 416 53 83 399 24 661 1226 629
Cuts at: 3933 4891 442
Size: 958 1753
Fragments arranged by size:
1753 1226 958 661 629 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2543 3044 3271 4862 1239
Size: 1304 501 227 1591 2579
Fragments arranged by size:
2579 1591 1304 501 227
Bpu10I CC'TnA_GC
Cuts at: 3131 3470 3131
Size: 339 5863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 4327 5192 5490 2111
Size: 2216 865 298 2823
Fragments arranged by size:
2823 2216 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2805 4865 882
Size: 617 1306 2060 2219
Fragments arranged by size:
2219 2060 1306 617
BsaAI yAC'GTr
Cuts at: 493 1204 3993 493
Size: 711 2789 2702
Fragments arranged by size:
2789 2702 711
BsaBI GATnn'nnATC
Cuts at: 6196 6196
Size: 6202
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4009 4391 5026 275
Size: 53 83 186 936 2476 382 635 1451
Fragments arranged by size:
2476 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4635 5033 5307 5454 5865 4635
Size: 398 274 147 411 4972
Fragments arranged by size:
4972 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5977
Fragments arranged by size:
5977 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2199 2552 3420 3458 1186
Size: 1013 353 868 38 3930
Fragments arranged by size:
3930 1013 868 353 38
BseYI C'CCAG_C
Cuts at: 2089 2579 2749 2776 5405 5901 2089
Size: 490 170 27 2629 496 2390
Fragments arranged by size:
2629 2390 496 490 170 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2007 2660 1163
Size: 573 271 653 4705
Fragments arranged by size:
4705 653 573 271
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2404 3054 3652 4413 5441 1540
Size: 275 186 403 650 598 761 1028 2301
Fragments arranged by size:
2301 1028 761 650 598 403 275 186
BsiHKAI G_wGCw'C
Cuts at: 2697 3362 3386 4263 4348 5419 2697
Size: 665 24 877 85 1071 3480
Fragments arranged by size:
3480 1071 877 665 85 24
BsmI GAATG_Cn'
Cuts at: 2184 3713 3806 2184
Size: 1529 93 4580
Fragments arranged by size:
4580 1529 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2145 2288 2447 2461 2580 2697
Size: 399 264 91 143 159 14 119 117
Cuts at: 2697 2733 2849 3148 3230 3362 3386 3440 3521
Size: 36 116 299 82 132 24 54 81
Cuts at: 3521 4263 4348 5419 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 299 264 159 143
132 119 117 116 91 85 82 81
54 36 24 14
BspEI T'CCGG_A
Cuts at: 5865 5865
Size: 6202
BspHI T'CATG_A
Cuts at: 3015 4091 5821 3015
Size: 1076 1730 3396
Fragments arranged by size:
3396 1730 1076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2565 2652 2970 3369 3557 844
Size: 1721 87 318 399 188 3489
Fragments arranged by size:
3489 1721 399 318 188 87
BsrBI CCG'CTC
Cuts at: 1394 1823 4089 1394
Size: 429 2266 3507
Fragments arranged by size:
3507 2266 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4697 4871 65
Size: 1183 3449 174 1396
Fragments arranged by size:
3449 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2304 2403 2981 4846 5033 1700
Size: 110 494 99 578 1865 187 2869
Fragments arranged by size:
2869 1865 578 494 187 110 99
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6202
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6202
BssSI C'ACGA_G
Cuts at: 2486 4262 5274 2486
Size: 1776 1012 3414
Fragments arranged by size:
3414 1776 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2233 2947 3160 794
Size: 1439 714 213 3836
Fragments arranged by size:
3836 1439 714 213
BstBI TT'CG_AA
Cuts at: 5006 5006
Size: 6202
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6202
BstYI r'GATC_y
Cuts at: 1972 3532 3899 4284 4301 5753 5839 5851 6197
Size: 1560 367 385 17 1452 86 12 346
Cuts at: 6197 1972
Size: 1977
Fragments arranged by size:
1977 1560 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2622 3330 2622
Size: 708 5494
BtgI C'CryG_G
Cuts at: 513 1065 2043 2356 2923 3048 3089 513
Size: 552 978 313 567 125 41 3626
Fragments arranged by size:
3626 978 567 552 313 125 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5463
Fragments arranged by size:
5463 683 56
BtsI GCAGTG_nn'
Cuts at: 2976 3068 3718 4512 4532 2976
Size: 92 650 794 20 4646
Fragments arranged by size:
4646 794 650 92 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6167
DraI TTT'AAA
Cuts at: 3506 3506
Size: 6202
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5209 809
Size: 507 3893 1802
Fragments arranged by size:
3893 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2306 2401
Size: 54 1425 50 275 18 198 278 95
Cuts at: 2401 2469 3051 3649 4538 6167 8
Size: 68 582 598 889 1629 43
Fragments arranged by size:
1629 1425 889 598 582 278 275 198
95 68 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2401 3051 3649 1537
Size: 275 589 650 598 4090
Fragments arranged by size:
4090 650 598 589 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2207 4132 1353
Size: 854 1925 3423
Fragments arranged by size:
3423 1925 854
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2090 2154 4789 5163 5309 387
Size: 1064 138 501 64 2635 374 146 1280
Fragments arranged by size:
2635 1280 1064 501 374 146 138 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2543 3044 3271 4265 4862 5649 5917
Size: 393 911 501 227 994 597 787 268
Cuts at: 5917 1239
Size: 1524
Fragments arranged by size:
1524 994 911 787 597 501 393 268
227
EcoNI CCTnn'n_nnAGG
Cuts at: 2621 3152 3479 2621
Size: 531 327 5344
Fragments arranged by size:
5344 531 327
EcoO109I rG'GnC_Cy
Cuts at: 2443 2444 2686 2729 2730 3058 3127 3145 3157
Size: 1 242 43 1 328 69 18 12
Cuts at: 3157 3314 2443
Size: 157 5331
Fragments arranged by size:
5331 328 242 157 69 43 18 12
1 1
EcoRI G'AATT_C
Cuts at: 3511 3889 3511
Size: 378 5824
FspI TGC'GCA
Cuts at: 4708 4708
Size: 6202
HaeII r_GCGC'y
Cuts at: 1678 5349 1678
Size: 3671 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2109 2141 3404 3436 4843
Size: 32 135 32 537 32 1263 32 1407
Cuts at: 4843 4875 4917 4949 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1407 1263 537 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3546 3792 669
Size: 988 281 1608 246 3079
Fragments arranged by size:
3079 1608 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6202
HpaI GTT'AAC
Cuts at: 3792 3792
Size: 6202
KpnI G_GTAC'C
Cuts at: 3527 3527
Size: 6202
MfeI C'AATT_G
Cuts at: 3801 3801
Size: 6202
MluI A'CGCG_T
Cuts at: 6078 6078
Size: 6202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5316 5500 1305
Size: 4011 184 2007
Fragments arranged by size:
4011 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2030 10
Size: 54 1425 343 198 4182
Fragments arranged by size:
4182 1425 343 198 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2700 4160 4519 4678 518
Size: 648 870 664 1460 359 159 2042
Fragments arranged by size:
2042 1460 870 664 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2233 2742 2864 2947 3091 3122 3210 3618
Size: 627 509 122 83 144 31 88 408
Cuts at: 3618 4295 5443 5688 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 627 509 408 245 144
122 88 83 31
NaeI GCC'GGC
Cuts at: 2306 2405 2306
Size: 99 6103
NcoI C'CATG_G
Cuts at: 513 1065 2356 513
Size: 552 1291 4359
Fragments arranged by size:
4359 1291 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6202
NgoMIV G'CCGG_C
Cuts at: 2304 2403 2304
Size: 99 6103
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6202
NotI GC'GGCC_GC
Cuts at: 3649 3649
Size: 6202
NspI r_CATG'y
Cuts at: 1132 1229 2907 3560 5105 1132
Size: 97 1678 653 1545 2229
Fragments arranged by size:
2229 1678 1545 653 97
PasI CC'CwG_GG
Cuts at: 1348 2136 2288 3223 3390 1348
Size: 788 152 935 167 4160
Fragments arranged by size:
4160 935 788 167 152
PciI A'CATG_T
Cuts at: 5101 5101
Size: 6202
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2689 3294 1108
Size: 1581 605 4016
Fragments arranged by size:
4016 1581 605
PfoI T'CCnGG_A
Cuts at: 2802 2892 3270 2802
Size: 90 378 5734
Fragments arranged by size:
5734 378 90
PmeI GTTT'AAAC
Cuts at: 3506 3506
Size: 6202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4271 4303 5784 5816 4271
Size: 32 1481 32 4657
Fragments arranged by size:
4657 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6202
PsiI TTA'TAA
Cuts at: 3772 3772
Size: 6202
PspOMI G'GGCC_C
Cuts at: 2443 2457 2576 2729 3144 3226 2443
Size: 14 119 153 415 82 5419
Fragments arranged by size:
5419 415 153 119 82 14
PstI C_TGCA'G
Cuts at: 830 2238 2283 2562 2589 2844 3263 3554 830
Size: 1408 45 279 27 255 419 291 3478
Fragments arranged by size:
3478 1408 419 291 279 255 45 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6202
PvuII CAG'CTG
Cuts at: 2233 2947 3210 2233
Size: 714 263 5225
Fragments arranged by size:
5225 714 263
SacII CC_GC'GG
Cuts at: 3092 3092
Size: 6202
SalI G'TCGA_C
Cuts at: 1936 3544 1936
Size: 1608 4594
SbfI CC_TGCA'GG
Cuts at: 3554 3554
Size: 6202
ScaI AGT'ACT
Cuts at: 4450 6162 4450
Size: 1712 4490
SfcI C'TryA_G
Cuts at: 826 1045 2234 2279 2558 2585 2840 3215 3259
Size: 219 1189 45 279 27 255 375 44
Cuts at: 3259 3550 4685 5366 5557 826
Size: 291 1135 681 191 1471
Fragments arranged by size:
1471 1189 1135 681 375 291 279 255
219 191 45 44 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6202
SmaI CCC'GGG
Cuts at: 1872 2735 3529 1872
Size: 863 794 4545
Fragments arranged by size:
4545 863 794
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2126 4306 5207 5469 820
Size: 197 926 183 2180 901 262 1553
Fragments arranged by size:
2180 1553 926 901 262 197 183
SnaBI TAC'GTA
Cuts at: 493 3993 493
Size: 3500 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6202
SphI G_CATG'C
Cuts at: 1132 2907 3560 1132
Size: 1775 653 3774
Fragments arranged by size:
3774 1775 653
SspI AAT'ATT
Cuts at: 5 52 4126 5
Size: 47 4074 2081
Fragments arranged by size:
4074 2081 47
StuI AGG'CCT
Cuts at: 1517 2267 2531 2603 1517
Size: 750 264 72 5116
Fragments arranged by size:
5116 750 264 72
StyI C'CwwG_G
Cuts at: 513 1065 2175 2356 2391 2448 3310 3641 513
Size: 552 1110 181 35 57 862 331 3074
Fragments arranged by size:
3074 1110 862 552 331 181 57 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2520 2741 3403 4229 4246 4399 4584
Size: 1255 940 221 662 826 17 153 185
Cuts at: 4584 5029 325
Size: 445 1498
Fragments arranged by size:
1498 1255 940 826 662 445 221 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4448 6160 96
Size: 274 80 33 51 1443 2471 1712 138
Fragments arranged by size:
2471 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2754 2786 6162 6194 2754
Size: 32 3376 32 2762
Fragments arranged by size:
3376 2762 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6202
XbaI T'CTAG_A
Cuts at: 3538 3538
Size: 6202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2111 2722 2896 3397 2111
Size: 611 174 501 4916
Fragments arranged by size:
4916 611 501 174
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6202
XmaI C'CCGG_G
Cuts at: 1870 2733 3527 1870
Size: 863 794 4545
Fragments arranged by size:
4545 863 794
XmnI GAAnn'nnTTC
Cuts at: 4331 4331
Size: 6202
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4010 276
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI