Restriction map for
pFN21AE0956
(Circular) (Six-base) MAPSORT of: pFN21AE0956.seq Check: 9567 from: 1 to: 6163
pFN21AE0956 6163 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2556 2556
Size: 6163
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3973 278
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3506 1937
Size: 1569 4594
Acc65I G'GTAC_C
Cuts at: 3484 3484
Size: 6163
AclI AA'CG_TT
Cuts at: 1563 4290 4663 1563
Size: 2727 373 3063
Fragments arranged by size:
3063 2727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3347 4226 5610 5878 1632
Size: 1715 879 1384 268 1917
Fragments arranged by size:
1917 1715 1384 879 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5966
AflIII A'CryG_T
Cuts at: 1203 2499 5062 6039 1203
Size: 1296 2563 977 1327
Fragments arranged by size:
2563 1327 1296 977
AgeI A'CCGG_T
Cuts at: 4994 4994
Size: 6163
AhdI GACnn_n'nnGTC
Cuts at: 2839 4892 2839
Size: 2053 4110
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2486 2518 2486
Size: 32 6131
AlwNI CAG_nnn'CTG
Cuts at: 3906 5478 3906
Size: 1572 4591
ApaI G_GGCC'C
Cuts at: 2140 2140
Size: 6163
ApaLI G'TGCA_C
Cuts at: 4220 5376 4220
Size: 1156 5007
ApoI r'AATT_y
Cuts at: 1493 3472 3698 3850 5959 1493
Size: 1979 226 152 2109 1697
Fragments arranged by size:
2109 1979 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4717 160
Size: 872 3685 1606
Fragments arranged by size:
3685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
AvaI C'yCGr_G
Cuts at: 1870 1943 2050 2133 2139 2146 3014 3225 3346
Size: 73 107 83 6 7 868 211 121
Cuts at: 3346 3488 1870
Size: 142 4545
Fragments arranged by size:
4545 868 211 142 121 107 83 73
7 6
BamHI G'GATC_C
Cuts at: 3493 3860 3493
Size: 367 5796
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2015 2759 3484 4939 618
Size: 325 217 627 228 744 725 1455 1842
Fragments arranged by size:
1842 1455 744 725 627 325 228 217
BanII G_rGCy'C
Cuts at: 1391 2057 2140 2213 3482 1391
Size: 666 83 73 1269 4072
Fragments arranged by size:
4072 1269 666 83 73
BbeI G_GCGC'C
Cuts at: 2019 2019
Size: 6163
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2410 2449 928
Size: 971 511 39 4642
Fragments arranged by size:
4642 971 511 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4354 4388 1903
Size: 34 2417 34 3678
Fragments arranged by size:
3678 2417 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4055 5265 4055
Size: 1210 4953
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6163
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2085 4774 136
Size: 107 122 71 631 605 413 2689 1525
Fragments arranged by size:
2689 1525 631 605 413 122 107 71
BglII A'GATC_T
Cuts at: 2784 6158 2784
Size: 3374 2789
BlpI GC'TnA_GC
Cuts at: 3580 3580
Size: 6163
Bme1580I G_kGCm'C
Cuts at: 1790 2140 3120 4224 5380 1790
Size: 350 980 1104 1156 2573
Fragments arranged by size:
2573 1156 1104 980 350
BmgBI CAC'GTC
Cuts at: 1318 2266 2455 3043 1318
Size: 948 189 588 4438
Fragments arranged by size:
4438 948 588 189
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2534 3244 3894
Size: 416 53 83 399 24 1117 710 650
Cuts at: 3894 4852 442
Size: 958 1753
Fragments arranged by size:
1753 1117 958 710 650 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6163
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3372 3372
Size: 6163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 2884 3013 3020 3179 3194 4823 1239
Size: 1211 434 129 7 159 15 1629 2579
Fragments arranged by size:
2579 1629 1211 434 159 129 15 7
Bpu10I CC'TnA_GC
Cuts at: 3086 3086
Size: 6163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4288 5153 5451 4288
Size: 865 298 5000
Fragments arranged by size:
5000 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4826 882
Size: 617 3327 2219
Fragments arranged by size:
3327 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2964 3954 493
Size: 711 1760 990 2702
Fragments arranged by size:
2702 1760 990 711
BsaBI GATnn'nnATC
Cuts at: 6157 6157
Size: 6163
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2016 2045 3970 4352
Size: 53 83 186 936 483 29 1925 382
Cuts at: 4352 4987 275
Size: 635 1451
Fragments arranged by size:
1925 1451 936 635 483 382 186 83
53 29
BsaWI w'CCGG_w
Cuts at: 2779 4596 4994 5268 5415 5826 2779
Size: 1817 398 274 147 411 3116
Fragments arranged by size:
3116 1817 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2342 2372 2486 2516 2882
Size: 30 165 30 939 30 114 30 366
Cuts at: 2882 2912 3011 3041 1178
Size: 30 99 30 4300
Fragments arranged by size:
4300 939 366 165 114 99 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2507 2990 3176 3346 1186
Size: 837 484 483 186 170 4003
Fragments arranged by size:
4003 837 484 483 186 170
BseYI C'CCAG_C
Cuts at: 5366 5862 5366
Size: 496 5667
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 2582 2945 3065 3119 3173 1163
Size: 573 618 228 363 120 54 54 4153
Fragments arranged by size:
4153 618 573 363 228 120 54 54
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2071 3453 3613 4374 5402 1540
Size: 275 186 70 1382 160 761 1028 2301
Fragments arranged by size:
2301 1382 1028 761 275 186 160 70
BsiHKAI G_wGCw'C
Cuts at: 2454 3021 4224 4309 5380 2454
Size: 567 1203 85 1071 3237
Fragments arranged by size:
3237 1203 1071 567 85
BsmI GAATG_Cn'
Cuts at: 3674 3767 3674
Size: 93 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2140 2213 2454 3021 3120 3482
Size: 399 267 83 73 241 567 99 362
Cuts at: 3482 4224 4309 5380 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 399 362 267 241
99 85 83 73
BspEI T'CCGG_A
Cuts at: 2779 5826 2779
Size: 3047 3116
BspHI T'CATG_A
Cuts at: 4052 5782 4052
Size: 1730 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2526 2544 2556 2631 3518 844
Size: 1682 18 12 75 887 3489
Fragments arranged by size:
3489 1682 887 75 18 12
BsrBI CCG'CTC
Cuts at: 1394 1823 2160 2740 3459 4050 1394
Size: 429 337 580 719 591 3507
Fragments arranged by size:
3507 719 591 580 429 337
BsrDI GCAATG_nn'
Cuts at: 65 1248 2712 4658 4832 65
Size: 1183 1464 1946 174 1396
Fragments arranged by size:
1946 1464 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2084 2242 3021 3065 4807 4994 1700
Size: 110 274 158 779 44 1742 187 2869
Fragments arranged by size:
2869 1742 779 274 187 158 110 44
BsrGI T'GTAC_A
Cuts at: 96 2589 96
Size: 2493 3670
BssHII G'CGCG_C
Cuts at: 1925 2073 2075 1925
Size: 148 2 6013
Fragments arranged by size:
6013 148 2
BssSI C'ACGA_G
Cuts at: 4223 5235 4223
Size: 1012 5151
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2325 2560 794
Size: 1531 235 4397
Fragments arranged by size:
4397 1531 235
BstBI TT'CG_AA
Cuts at: 4967 4967
Size: 6163
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6163
BstXI CCAn_nnnn'nTGG
Cuts at: 2977 2977
Size: 6163
BstYI r'GATC_y
Cuts at: 1972 2286 2433 2784 3493 3860 4245 4262 5714
Size: 314 147 351 709 367 385 17 1452
Cuts at: 5714 5800 5812 6158 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 709 385 367 351 346 314
147 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2040 2910 513
Size: 552 975 870 3766
Fragments arranged by size:
3766 975 870 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2166 487
Size: 683 56 940 4484
Fragments arranged by size:
4484 940 683 56
BtsI GCAGTG_nn'
Cuts at: 2393 3136 3679 4473 4493 2393
Size: 743 543 794 20 4063
Fragments arranged by size:
4063 794 743 543 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6128
DraI TTT'AAA
Cuts at: 3467 3467
Size: 6163
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5170 809
Size: 507 3854 1802
Fragments arranged by size:
3854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2068 2125 2226
Size: 54 1425 50 275 18 238 57 101
Cuts at: 2226 2629 3063 3210 3450 3610 4499 6128 8
Size: 403 434 147 240 160 889 1629 43
Fragments arranged by size:
1629 1425 889 434 403 275 240 238
160 147 101 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2068 3450 3610 1537
Size: 275 256 1382 160 4090
Fragments arranged by size:
4090 1382 275 256 160
EarI CTCTTCn'nnn_
Cuts at: 1353 2195 3089 3323 4093 1353
Size: 842 894 234 770 3423
Fragments arranged by size:
3423 894 842 770 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3406 3422 4750 5124 5270 387
Size: 1064 138 1817 16 1328 374 146 1280
Fragments arranged by size:
1817 1328 1280 1064 374 146 138 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 2884 3013 3020 3179 3194 3347
Size: 393 818 434 129 7 159 15 153
Cuts at: 3347 4226 4823 5610 5878 1239
Size: 879 597 787 268 1524
Fragments arranged by size:
1524 879 818 787 597 434 393 268
159 153 129 15 7
EcoO109I rG'GnC_Cy
Cuts at: 2533 2883 3183 3340 2533
Size: 350 300 157 5356
Fragments arranged by size:
5356 350 300 157
EcoRI G'AATT_C
Cuts at: 3472 3850 3472
Size: 378 5785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 6163
FspI TGC'GCA
Cuts at: 4669 4669
Size: 6163
HaeII r_GCGC'y
Cuts at: 1678 2019 2040 3062 3208 5310 1678
Size: 341 21 1022 146 2102 2531
Fragments arranged by size:
2531 2102 1022 341 146 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2144 2176 2825
Size: 32 135 32 512 32 28 32 649
Cuts at: 2825 2857 2882 2914 4804 4836 4878 4910 1373
Size: 32 25 32 1890 32 42 32 2626
Fragments arranged by size:
2626 1890 649 512 135 42 32 32
32 32 32 32 32 32 28 25
HincII GTy'rAC
Cuts at: 669 1657 1938 2803 3507 3753 669
Size: 988 281 865 704 246 3079
Fragments arranged by size:
3079 988 865 704 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6163
HpaI GTT'AAC
Cuts at: 3753 3753
Size: 6163
KasI G'GCGC_C
Cuts at: 2015 2015
Size: 6163
KpnI G_GTAC'C
Cuts at: 3488 3488
Size: 6163
MfeI C'AATT_G
Cuts at: 3762 3762
Size: 6163
MluI A'CGCG_T
Cuts at: 6039 6039
Size: 6163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2115 5277 5461 1305
Size: 810 3162 184 2007
Fragments arranged by size:
3162 2007 810 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2127 3212 10
Size: 54 1425 343 295 1085 2961
Fragments arranged by size:
2961 1425 1085 343 295 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2504 2510 2649 4121 4480 4639 518
Size: 648 1338 6 139 1472 359 159 2042
Fragments arranged by size:
2042 1472 1338 648 359 159 139 6
MspA1I CmG'CkG
Cuts at: 1606 2042 2120 2929 3579 4256 5404 5649 1606
Size: 436 78 809 650 677 1148 245 2120
Fragments arranged by size:
2120 1148 809 677 650 436 245 78
NaeI GCC'GGC
Cuts at: 2086 3067 2086
Size: 981 5182
NarI GG'CG_CC
Cuts at: 2016 2016
Size: 6163
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5611
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6163
NgoMIV G'CCGG_C
Cuts at: 2084 3065 2084
Size: 981 5182
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6163
NotI GC'GGCC_GC
Cuts at: 3610 3610
Size: 6163
NspI r_CATG'y
Cuts at: 1132 1229 2503 2693 3521 5066 1132
Size: 97 1274 190 828 1545 2229
Fragments arranged by size:
2229 1545 1274 828 190 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6163
PciI A'CATG_T
Cuts at: 2499 5062 2499
Size: 2563 3600
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6163
PfoI T'CCnGG_A
Cuts at: 2421 2484 2673 3036 3171 2421
Size: 63 189 363 135 5413
Fragments arranged by size:
5413 363 189 135 63
PmeI GTTT'AAAC
Cuts at: 3467 3467
Size: 6163
PmlI CAC'GTG
Cuts at: 2964 2964
Size: 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2486 2518 4232 4264 5745 5777 2486
Size: 32 1714 32 1481 32 2872
Fragments arranged by size:
2872 1714 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2533 3340 2533
Size: 807 5356
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6163
PsiI TTA'TAA
Cuts at: 3733 3733
Size: 6163
PspOMI G'GGCC_C
Cuts at: 2136 2136
Size: 6163
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2459 2491 2459
Size: 32 6131
PstI C_TGCA'G
Cuts at: 830 2442 2553 3515 830
Size: 1612 111 962 3478
Fragments arranged by size:
3478 1612 962 111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6163
PvuII CAG'CTG
Cuts at: 2929 2929
Size: 6163
SacII CC_GC'GG
Cuts at: 2043 2043
Size: 6163
SalI G'TCGA_C
Cuts at: 1936 3505 1936
Size: 1569 4594
SbfI CC_TGCA'GG
Cuts at: 2442 3515 2442
Size: 1073 5090
ScaI AGT'ACT
Cuts at: 4411 6123 4411
Size: 1712 4451
SfcI C'TryA_G
Cuts at: 826 1045 2438 2549 3511 4646 5327 5518 826
Size: 219 1393 111 962 1135 681 191 1471
Fragments arranged by size:
1471 1393 1135 962 681 219 191 111
SfoI GGC'GCC
Cuts at: 2017 2017
Size: 6163
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6163
SmaI CCC'GGG
Cuts at: 1872 2052 2135 2141 3227 3490 1872
Size: 180 83 6 1086 263 4545
Fragments arranged by size:
4545 1086 263 180 83 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4267 5168 5430 820
Size: 197 926 2324 901 262 1553
Fragments arranged by size:
2324 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3954 493
Size: 3461 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6163
SphI G_CATG'C
Cuts at: 1132 3521 1132
Size: 2389 3774
SspI AAT'ATT
Cuts at: 5 52 4087 5
Size: 47 4035 2081
Fragments arranged by size:
4035 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6163
StyI C'CwwG_G
Cuts at: 513 1065 2970 3602 513
Size: 552 1905 632 3074
Fragments arranged by size:
3074 1905 632 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2717 4190 4207 4360 4545 4990 325
Size: 1255 1137 1473 17 153 185 445 1498
Fragments arranged by size:
1498 1473 1255 1137 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2370 2589 4409
Size: 274 80 33 51 1443 393 219 1820
Cuts at: 4409 6121 96
Size: 1712 138
Fragments arranged by size:
1820 1712 1443 393 274 219 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 6123 6155 3011
Size: 32 3080 32 3019
Fragments arranged by size:
3080 3019 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2405 1316
Size: 1089 5074
XbaI T'CTAG_A
Cuts at: 3499 3499
Size: 6163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2770 2908 2977 3154 2770
Size: 138 69 177 5779
Fragments arranged by size:
5779 177 138 69
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6163
XmaI C'CCGG_G
Cuts at: 1870 2050 2133 2139 3225 3488 1870
Size: 180 83 6 1086 263 4545
Fragments arranged by size:
4545 1086 263 180 83 6
XmnI GAAnn'nnTTC
Cuts at: 4292 4292
Size: 6163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3971 276
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI
NruI NsiI PacI PspXI RsrII SacI SanDI SapI
SexAI SfiI SrfI SwaI