Restriction map for
pFN21AE0954
(Circular) (Six-base) MAPSORT of: pFN21AE0954.seq Check: 5837 from: 1 to: 6151
pFN21AE0954 6151 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3494 1937
Size: 1557 4594
Acc65I G'GTAC_C
Cuts at: 3472 3472
Size: 6151
AclI AA'CG_TT
Cuts at: 1563 2172 4278 4651 1563
Size: 609 2106 373 3063
Fragments arranged by size:
3063 2106 609 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4214 5598 5866 1632
Size: 2582 1384 268 1917
Fragments arranged by size:
2582 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 5050 6027 1203
Size: 3847 977 1327
Fragments arranged by size:
3847 1327 977
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 3170 1166
Size: 2004 4147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6151
AlwNI CAG_nnn'CTG
Cuts at: 3045 3894 5466 3045
Size: 849 1572 3730
Fragments arranged by size:
3730 1572 849
ApaI G_GGCC'C
Cuts at: 2196 2776 2196
Size: 580 5571
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 3460 3686 3838 5947 1493
Size: 1967 226 152 2109 1697
Fragments arranged by size:
2109 1967 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 2594 2892 3258 3476 1870
Size: 73 651 298 366 218 4545
Fragments arranged by size:
4545 651 366 298 218 73
BamHI G'GATC_C
Cuts at: 3481 3848 3481
Size: 367 5784
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2270 2672 2696 3175 3240
Size: 325 217 627 483 402 24 479 65
Cuts at: 3240 3472 4927 618
Size: 232 1455 1842
Fragments arranged by size:
1842 1455 627 483 479 402 325 232
217 65 24
BanII G_rGCy'C
Cuts at: 1391 2073 2196 2472 2776 3330 3470 1391
Size: 682 123 276 304 554 140 4072
Fragments arranged by size:
4072 682 554 304 276 140 123
BbeI G_GCGC'C
Cuts at: 2676 3179 3244 2676
Size: 503 65 5583
Fragments arranged by size:
5583 503 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5180
BbvCI CC'TCA_GC
Cuts at: 3131 3131
Size: 6151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4342 4376 1903
Size: 34 2405 34 3678
Fragments arranged by size:
3678 2405 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4043 5253 4043
Size: 1210 4941
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3149 4762
Size: 107 122 71 631 605 337 1140 1613
Cuts at: 4762 136
Size: 1525
Fragments arranged by size:
1613 1525 1140 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 6146 6146
Size: 6151
BlpI GC'TnA_GC
Cuts at: 2072 2358 2609 3568 2072
Size: 286 251 959 4655
Fragments arranged by size:
4655 959 286 251
Bme1580I G_kGCm'C
Cuts at: 1790 2196 2776 4212 5368 1790
Size: 406 580 1436 1156 2573
Fragments arranged by size:
2573 1436 1156 580 406
BmgBI CAC'GTC
Cuts at: 1318 3067 1318
Size: 1749 4402
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3882 4840 442
Size: 416 53 83 399 24 2465 958 1753
Fragments arranged by size:
2465 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3226 3226
Size: 6151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2504 4811 1239
Size: 1265 2307 2579
Fragments arranged by size:
2579 2307 1265
Bpu10I CC'TnA_GC
Cuts at: 3131 3131
Size: 6151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2468 2573 3263 4276 5141 5439 2468
Size: 105 690 1013 865 298 3180
Fragments arranged by size:
3180 1013 865 690 298 105
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2153 4814 882
Size: 617 654 2661 2219
Fragments arranged by size:
2661 2219 654 617
BsaAI yAC'GTr
Cuts at: 493 1204 3172 3942 493
Size: 711 1968 770 2702
Fragments arranged by size:
2702 1968 770 711
BsaBI GATnn'nnATC
Cuts at: 6145 6145
Size: 6151
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2673 3101 3176 3241
Size: 53 83 186 936 1140 428 75 65
Cuts at: 3241 3439 3958 4340 4975 275
Size: 198 519 382 635 1451
Fragments arranged by size:
1451 1140 936 635 519 428 382 198
186 83 75 65 53
BsaWI w'CCGG_w
Cuts at: 2823 4584 4982 5256 5403 5814 2823
Size: 1761 398 274 147 411 3160
Fragments arranged by size:
3160 1761 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3336 3366 1178
Size: 30 165 30 1933 30 3963
Fragments arranged by size:
3963 1933 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3074 1186
Size: 1888 4263
BseYI C'CCAG_C
Cuts at: 2221 2927 3308 3314 5354 5850 2221
Size: 706 381 6 2040 496 2522
Fragments arranged by size:
2522 2040 706 496 381 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2990 1163
Size: 573 1254 4324
Fragments arranged by size:
4324 1254 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 3601 4362 5390 1540
Size: 275 186 12 1588 761 1028 2301
Fragments arranged by size:
2301 1588 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2073 2472 4212 4297 5368 2073
Size: 399 1740 85 1071 2856
Fragments arranged by size:
2856 1740 1071 399 85
BsmI GAATG_Cn'
Cuts at: 3662 3755 3662
Size: 93 6058
BsmBI CGTCTCn'nnnn_
Cuts at: 2027 2027
Size: 6151
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2196 2472 2776 3330 3470 4212
Size: 399 283 123 276 304 554 140 742
Cuts at: 4212 4297 5368 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 554 399 304 283 276
140 123 85
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 4040 5770 4040
Size: 1730 4421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3506 844
Size: 2662 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2362 4038 1394
Size: 429 539 1676 3507
Fragments arranged by size:
3507 1676 539 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2335 4646 4820 65
Size: 1183 1087 2311 174 1396
Fragments arranged by size:
2311 1396 1183 1087 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3103 4795 4982 1700
Size: 110 1293 1692 187 2869
Fragments arranged by size:
2869 1692 1293 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 4211 5223 4211
Size: 1012 5139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6151
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6151
BstXI CCAn_nnnn'nTGG
Cuts at: 2426 2939 2426
Size: 513 5638
BstYI r'GATC_y
Cuts at: 1972 3481 3848 4233 4250 5702 5788 5800 6146
Size: 1509 367 385 17 1452 86 12 346
Cuts at: 6146 1972
Size: 1977
Fragments arranged by size:
1977 1509 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2306 3406 2306
Size: 1100 5051
BtgI C'CryG_G
Cuts at: 513 1065 2004 2079 2835 3330 3433 513
Size: 552 939 75 756 495 103 3231
Fragments arranged by size:
3231 939 756 552 495 103 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5412
Fragments arranged by size:
5412 683 56
BtsI GCAGTG_nn'
Cuts at: 2197 2740 2864 3667 4461 4481 2197
Size: 543 124 803 794 20 3867
Fragments arranged by size:
3867 803 794 543 124 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 3455 3455
Size: 6151
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3418 5158 809
Size: 507 2102 1740 1802
Fragments arranged by size:
2102 1802 1740 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2737 3598
Size: 54 1425 50 275 18 180 727 861
Cuts at: 3598 4487 6116 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 861 727 275 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 3598 1537
Size: 275 198 1588 4090
Fragments arranged by size:
4090 1588 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2136 2477 4081 1353
Size: 783 341 1604 3423
Fragments arranged by size:
3423 1604 783 341
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4738 5112 5258 387
Size: 1064 138 3149 374 146 1280
Fragments arranged by size:
3149 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2071 2470 2071
Size: 399 5752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2504 4214 4811 5598 5866 1239
Size: 393 872 1710 597 787 268 1524
Fragments arranged by size:
1710 1524 872 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2309 2309
Size: 6151
EcoO109I rG'GnC_Cy
Cuts at: 2193 2584 2772 2773 2896 3042 3262 2193
Size: 391 188 1 123 146 220 5082
Fragments arranged by size:
5082 391 220 188 146 123 1
EcoRI G'AATT_C
Cuts at: 3460 3838 3460
Size: 378 5773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 3223 2407
Size: 816 5335
FspI TGC'GCA
Cuts at: 2327 2732 4657 2327
Size: 405 1925 3821
Fragments arranged by size:
3821 1925 405
HaeII r_GCGC'y
Cuts at: 1678 2676 3179 3244 5298 1678
Size: 998 503 65 2054 2531
Fragments arranged by size:
2531 2054 998 503 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2481 2513 4792 4824 4866
Size: 32 135 32 909 32 2279 32 42
Cuts at: 4866 4898 1373
Size: 32 2626
Fragments arranged by size:
2626 2279 909 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3495 3741 669
Size: 988 281 1557 246 3079
Fragments arranged by size:
3079 1557 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6151
HpaI GTT'AAC
Cuts at: 3741 3741
Size: 6151
KasI G'GCGC_C
Cuts at: 2672 3175 3240 2672
Size: 503 65 5583
Fragments arranged by size:
5583 503 65
KpnI G_GTAC'C
Cuts at: 3476 3476
Size: 6151
MfeI C'AATT_G
Cuts at: 3750 3750
Size: 6151
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5265 5449 1305
Size: 3960 184 2007
Fragments arranged by size:
3960 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2739 10
Size: 54 1425 343 907 3422
Fragments arranged by size:
3422 1425 907 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3170 4109 4468 4627 518
Size: 648 2004 939 359 159 2042
Fragments arranged by size:
2042 2004 939 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2081 2114 2935 3567 4244 5392 5637 1606
Size: 475 33 821 632 677 1148 245 2120
Fragments arranged by size:
2120 1148 821 677 632 475 245 33
NarI GG'CG_CC
Cuts at: 2673 3176 3241 2673
Size: 503 65 5583
Fragments arranged by size:
5583 503 65
NcoI C'CATG_G
Cuts at: 513 1065 2004 2835 513
Size: 552 939 831 3829
Fragments arranged by size:
3829 939 831 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 2091 2791 3509 5054 1132
Size: 97 862 700 718 1545 2229
Fragments arranged by size:
2229 1545 862 718 700 97
PasI CC'CwG_GG
Cuts at: 1348 2548 2566 2951 1348
Size: 1200 18 385 4548
Fragments arranged by size:
4548 1200 385 18
PciI A'CATG_T
Cuts at: 5050 5050
Size: 6151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6151
PfoI T'CCnGG_A
Cuts at: 2502 3186 2502
Size: 684 5467
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4220 4252 5733 5765 4220
Size: 32 1481 32 4606
Fragments arranged by size:
4606 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2584 2896 3262 2584
Size: 312 366 5473
Fragments arranged by size:
5473 366 312
PshAI GACnn'nnGTC
Cuts at: 1613 2088 1613
Size: 475 5676
PsiI TTA'TAA
Cuts at: 3721 3721
Size: 6151
PspOMI G'GGCC_C
Cuts at: 2192 2772 2192
Size: 580 5571
PstI C_TGCA'G
Cuts at: 830 3054 3503 830
Size: 2224 449 3478
Fragments arranged by size:
3478 2224 449
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
PvuII CAG'CTG
Cuts at: 2114 2114
Size: 6151
SacI G_AGCT'C
Cuts at: 2073 2472 2073
Size: 399 5752
SacII CC_GC'GG
Cuts at: 2082 2082
Size: 6151
SalI G'TCGA_C
Cuts at: 1936 3493 1936
Size: 1557 4594
SanDI GG'GwC_CC
Cuts at: 3262 3262
Size: 6151
SapI GCTCTTCn'nnn_
Cuts at: 2477 2477
Size: 6151
SbfI CC_TGCA'GG
Cuts at: 3503 3503
Size: 6151
ScaI AGT'ACT
Cuts at: 4399 6111 4399
Size: 1712 4439
SfcI C'TryA_G
Cuts at: 826 1045 3050 3499 4634 5315 5506 826
Size: 219 2005 449 1135 681 191 1471
Fragments arranged by size:
2005 1471 1135 681 449 219 191
SfoI GGC'GCC
Cuts at: 2674 3177 3242 2674
Size: 503 65 5583
Fragments arranged by size:
5583 503 65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 3103 1700
Size: 1403 4748
SmaI CCC'GGG
Cuts at: 1872 2894 3260 3478 1872
Size: 1022 366 218 4545
Fragments arranged by size:
4545 1022 366 218
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2447 2588 3278 4255 5156 5418
Size: 197 926 504 141 690 977 901 262
Cuts at: 5418 820
Size: 1553
Fragments arranged by size:
1553 977 926 901 690 504 262 197
141
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6151
SphI G_CATG'C
Cuts at: 1132 3509 1132
Size: 2377 3774
SspI AAT'ATT
Cuts at: 5 52 4075 5
Size: 47 4023 2081
Fragments arranged by size:
4023 2081 47
StuI AGG'CCT
Cuts at: 1517 3152 1517
Size: 1635 4516
StyI C'CwwG_G
Cuts at: 513 1065 2004 2334 2835 3590 513
Size: 552 939 330 501 755 3074
Fragments arranged by size:
3074 939 755 552 501 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2651 3250 4178 4195 4348 4533 4978
Size: 1255 1071 599 928 17 153 185 445
Cuts at: 4978 325
Size: 1498
Fragments arranged by size:
1498 1255 1071 928 599 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 4397 6109
Size: 274 80 33 51 1443 729 1691 1712
Cuts at: 6109 96
Size: 138
Fragments arranged by size:
1712 1691 1443 729 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2929 2961 6111 6143 2929
Size: 32 3150 32 2937
Fragments arranged by size:
3150 2937 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2900 1316
Size: 1584 4567
XbaI T'CTAG_A
Cuts at: 2371 3487 2371
Size: 1116 5035
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2707 2707
Size: 6151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 2892 3258 3476 1870
Size: 1022 366 218 4545
Fragments arranged by size:
4545 1022 366 218
XmnI GAAnn'nnTTC
Cuts at: 4280 4280
Size: 6151
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI
SrfI SwaI