Restriction map for
pFN21AE0953
(Circular) (Six-base) MAPSORT of: pFN21AE0953.seq Check: 1212 from: 1 to: 6148
pFN21AE0953 6148 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 3491 1937
Size: 1083 471 4594
Fragments arranged by size:
4594 1083 471
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2301 2885 4211 5595 5863 1632
Size: 669 584 1326 1384 268 1917
Fragments arranged by size:
1917 1384 1326 669 584 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 5047 6024 1203
Size: 3844 977 1327
Fragments arranged by size:
3844 1327 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6148
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2014 2046 2014
Size: 32 6116
AlwNI CAG_nnn'CTG
Cuts at: 3891 5463 3891
Size: 1572 4576
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 2827 3311 3457 3683 3835 5944 1493
Size: 1334 484 146 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 484 226 152 146
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 3041 3473 1870
Size: 73 1098 432 4545
Fragments arranged by size:
4545 1098 432 73
BamHI G'GATC_C
Cuts at: 3478 3845 3478
Size: 367 5781
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3469 4924 618
Size: 325 217 627 1682 1455 1842
Fragments arranged by size:
1842 1682 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3040 3467 1391
Size: 1649 427 4072
Fragments arranged by size:
4072 1649 427
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2653 928
Size: 971 754 4423
Fragments arranged by size:
4423 971 754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2286 2320 4339 4373 1903
Size: 34 50 34 265 34 2019 34 3678
Fragments arranged by size:
3678 2019 265 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4040 5250 4040
Size: 1210 4938
BclI T'GATC_A
Cuts at: 1548 2536 2692 1548
Size: 988 156 5004
Fragments arranged by size:
5004 988 156
BfrBI ATG'CAT
Cuts at: 2557 2557
Size: 6148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4759 136
Size: 107 122 71 631 605 3087 1525
Fragments arranged by size:
3087 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2099 2811 2860 6143 2099
Size: 712 49 3283 2104
Fragments arranged by size:
3283 2104 712 49
BlpI GC'TnA_GC
Cuts at: 3565 3565
Size: 6148
Bme1580I G_kGCm'C
Cuts at: 1790 4209 5365 1790
Size: 2419 1156 2573
Fragments arranged by size:
2573 2419 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2738 3879 4837
Size: 416 53 83 399 24 1321 1141 958
Cuts at: 4837 442
Size: 1753
Fragments arranged by size:
1753 1321 1141 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2118 2118
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4808 1239
Size: 3569 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4273 5138 5436 4273
Size: 865 298 4985
Fragments arranged by size:
4985 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3072 4811 882
Size: 617 1573 1739 2219
Fragments arranged by size:
2219 1739 1573 617
BsaAI yAC'GTr
Cuts at: 493 1204 3939 493
Size: 711 2735 2702
Fragments arranged by size:
2735 2702 711
BsaBI GATnn'nnATC
Cuts at: 3000 6142 3000
Size: 3142 3006
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3955 4337 4972 275
Size: 53 83 186 936 2422 382 635 1451
Fragments arranged by size:
2422 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2211 4581 4979 5253 5400 5811 2211
Size: 2370 398 274 147 411 2548
Fragments arranged by size:
2548 2370 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2323 2353 3079 3109 1178
Size: 30 165 30 920 30 726 30 4217
Fragments arranged by size:
4217 920 726 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2110 2447 1186
Size: 924 337 4887
Fragments arranged by size:
4887 924 337
BseYI C'CCAG_C
Cuts at: 2055 5351 5847 2055
Size: 3296 496 2356
Fragments arranged by size:
3296 2356 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5575
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3598 4359 5387 1540
Size: 275 186 1597 761 1028 2301
Fragments arranged by size:
2301 1597 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2634 3040 3159 4209 4294 5365 2634
Size: 406 119 1050 85 1071 3417
Fragments arranged by size:
3417 1071 1050 406 119 85
BsmI GAATG_Cn'
Cuts at: 2011 3099 3659 3752 2011
Size: 1088 560 93 4407
Fragments arranged by size:
4407 1088 560 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2454 3283 2454
Size: 829 5319
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2634 3040 3159 3467 4209 4294 5365
Size: 399 844 406 119 308 742 85 1071
Cuts at: 5365 1391
Size: 2174
Fragments arranged by size:
2174 1071 844 742 406 399 308 119
85
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 3142 3325 4037 5767 3142
Size: 183 712 1730 3523
Fragments arranged by size:
3523 1730 712 183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3503 844
Size: 2659 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4035 1394
Size: 429 2212 3507
Fragments arranged by size:
3507 2212 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3171 3410 4643 4817 65
Size: 1183 1923 239 1233 174 1396
Fragments arranged by size:
1923 1396 1233 1183 239 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4792 4979 1700
Size: 110 2982 187 2869
Fragments arranged by size:
2982 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 4208 5220 4208
Size: 1012 5136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3096 794
Size: 2302 3846
BstBI TT'CG_AA
Cuts at: 2387 3361 4952 2387
Size: 974 1591 3583
Fragments arranged by size:
3583 1591 974
BstEII G'GTnAC_C
Cuts at: 1184 2438 1184
Size: 1254 4894
BstYI r'GATC_y
Cuts at: 1972 2099 2408 2811 2860 3478 3845 4230 4247
Size: 127 309 403 49 618 367 385 17
Cuts at: 4247 5699 5785 5797 6143 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 618 403 385 367 346 309
127 86 49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5596
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5409
Fragments arranged by size:
5409 683 56
BtsI GCAGTG_nn'
Cuts at: 2302 2571 3664 4458 4478 2302
Size: 269 1093 794 20 3972
Fragments arranged by size:
3972 1093 794 269 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 2152 3452 2152
Size: 1300 4848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2903 5155 809
Size: 507 1587 2252 1802
Fragments arranged by size:
2252 1802 1587 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3595 4484 6113
Size: 54 1425 50 275 18 1765 889 1629
Cuts at: 6113 8
Size: 43
Fragments arranged by size:
1765 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3595 1537
Size: 275 1783 4090
Fragments arranged by size:
4090 1783 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2384 4078 1353
Size: 734 297 1694 3423
Fragments arranged by size:
3423 1694 734 297
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4735 5109 5255 387
Size: 1064 138 3146 374 146 1280
Fragments arranged by size:
3146 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3038 3038
Size: 6148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 2885 4211 4808 5595 5863 1239
Size: 393 669 584 1326 597 787 268 1524
Fragments arranged by size:
1524 1326 787 669 597 584 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2184 3185 2184
Size: 1001 5147
EcoRI G'AATT_C
Cuts at: 3457 3835 3457
Size: 378 5770
EcoRV GAT'ATC
Cuts at: 2959 2959
Size: 6148
FspI TGC'GCA
Cuts at: 4654 4654
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 5295 1678
Size: 3617 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2323 2355 2469 2501 2791
Size: 32 135 32 751 32 114 32 290
Cuts at: 2791 2823 4789 4821 4863 4895 1373
Size: 32 1966 32 42 32 2626
Fragments arranged by size:
2626 1966 751 290 135 114 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2224 2971 3492 3738 669
Size: 988 281 286 747 521 246 3079
Fragments arranged by size:
3079 988 747 521 286 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6148
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3407 5262 5446 1305
Size: 2102 1855 184 2007
Fragments arranged by size:
2102 2007 1855 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4326
Fragments arranged by size:
4326 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2703 4106 4465 4624 518
Size: 648 1537 1403 359 159 2042
Fragments arranged by size:
2042 1537 1403 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3564 4241 5389 5634 1606
Size: 1958 677 1148 245 2120
Fragments arranged by size:
2120 1958 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5596
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NsiI A_TGCA'T
Cuts at: 2559 2559
Size: 6148
NspI r_CATG'y
Cuts at: 1132 1229 2557 2561 3506 5051 1132
Size: 97 1328 4 945 1545 2229
Fragments arranged by size:
2229 1545 1328 945 97 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6148
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6148
PfoI T'CCnGG_A
Cuts at: 2411 2411
Size: 6148
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 5730 5762 4217
Size: 32 1481 32 4603
Fragments arranged by size:
4603 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2184 3185 2184
Size: 1001 5147
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6148
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PstI C_TGCA'G
Cuts at: 830 2309 3500 830
Size: 1479 1191 3478
Fragments arranged by size:
3478 1479 1191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
SacI G_AGCT'C
Cuts at: 3040 3040
Size: 6148
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SapI GCTCTTCn'nnn_
Cuts at: 2384 2384
Size: 6148
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 2644 2740 3010 4396 6108 2644
Size: 96 270 1386 1712 2684
Fragments arranged by size:
2684 1712 1386 270 96
SexAI A'CCwGG_T
Cuts at: 3354 3354
Size: 6148
SfcI C'TryA_G
Cuts at: 826 1045 2305 3031 3496 4631 5312 5503 826
Size: 219 1260 726 465 1135 681 191 1471
Fragments arranged by size:
1471 1260 1135 726 681 465 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 3475 1872
Size: 1603 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4252 5153 5415 820
Size: 197 926 2309 901 262 1553
Fragments arranged by size:
2309 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 2557 3506 1132
Size: 1425 949 3774
Fragments arranged by size:
3774 1425 949
SspI AAT'ATT
Cuts at: 5 52 2878 3113 3180 4072 5
Size: 47 2826 235 67 892 2081
Fragments arranged by size:
2826 2081 892 235 67 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6148
StyI C'CwwG_G
Cuts at: 513 1065 3118 3399 3587 513
Size: 552 2053 281 188 3074
Fragments arranged by size:
3074 2053 552 281 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2489 4175 4192 4345 4530 4975 325
Size: 1255 909 1686 17 153 185 445 1498
Fragments arranged by size:
1686 1498 1255 909 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2642 2738 3008
Size: 274 80 33 51 1443 665 96 270
Cuts at: 3008 4394 6106 96
Size: 1386 1712 138
Fragments arranged by size:
1712 1443 1386 665 274 270 138 96
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6108 6140 6108
Size: 32 6116
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6148
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 3473 1870
Size: 1603 4545
XmnI GAAnn'nnTTC
Cuts at: 4277 4277
Size: 6148
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SfiI SfoI SrfI SwaI XcmI