Restriction map for
pFN21AE0952
(Circular) (Six-base) MAPSORT of: pFN21AE0952.seq Check: 3647 from: 1 to: 6127
pFN21AE0952 6127 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2848 2848
Size: 6127
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3470 1937
Size: 1533 4594
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2280 2444 2528 2786 3167 3320 4190 5574
Size: 648 164 84 258 381 153 870 1384
Cuts at: 5574 5842 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 870 648 381 268 258 164
153 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 2125 3019 5026 6003 1203
Size: 922 894 2007 977 1327
Fragments arranged by size:
2007 1327 977 922 894
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3101 1184
Size: 1917 4210
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3053 3085 3053
Size: 32 6095
AlwNI CAG_nnn'CTG
Cuts at: 2422 3130 3382 3870 5442 2422
Size: 708 252 488 1572 3107
Fragments arranged by size:
3107 1572 708 488 252
ApaI G_GGCC'C
Cuts at: 3105 3243 3105
Size: 138 5989
ApaLI G'TGCA_C
Cuts at: 4184 5340 4184
Size: 1156 4971
ApoI r'AATT_y
Cuts at: 1493 2054 2074 3436 3662 3814 5923 1493
Size: 561 20 1362 226 152 2109 1697
Fragments arranged by size:
2109 1697 1362 561 226 152 20
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 3452 1870
Size: 73 1509 4545
Fragments arranged by size:
4545 1509 73
BamHI G'GATC_C
Cuts at: 2947 3457 3824 2947
Size: 510 367 5250
Fragments arranged by size:
5250 510 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3421 3448 4903 618
Size: 325 217 627 1634 27 1455 1842
Fragments arranged by size:
1842 1634 1455 627 325 217 27
BanII G_rGCy'C
Cuts at: 1391 2351 2414 3050 3105 3243 3446 1391
Size: 960 63 636 55 138 203 4072
Fragments arranged by size:
4072 960 636 203 138 63 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2342 2384 2405 928
Size: 971 443 42 21 4650
Fragments arranged by size:
4650 971 443 42 21
BbvCI CC'TCA_GC
Cuts at: 3350 3350
Size: 6127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2698 2725 2732 2759 4318 4352 1903
Size: 34 761 27 7 27 1559 34 3678
Fragments arranged by size:
3678 1559 761 34 34 27 27 7
BciVI GTATCCnnnnn_n'
Cuts at: 2048 4019 5229 2048
Size: 1971 1210 2946
Fragments arranged by size:
2946 1971 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3012 4738 136
Size: 107 122 71 631 605 1340 1726 1525
Fragments arranged by size:
1726 1525 1340 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 3544 3544
Size: 6127
Bme1580I G_kGCm'C
Cuts at: 1790 3105 3243 3426 4188 5344 1790
Size: 1315 138 183 762 1156 2573
Fragments arranged by size:
2573 1315 1156 762 183 138
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3858 4816 442
Size: 416 53 83 399 24 2441 958 1753
Fragments arranged by size:
2441 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2149 2906 3107 4787 1239
Size: 910 757 201 1680 2579
Fragments arranged by size:
2579 1680 910 757 201
Bpu10I CC'TnA_GC
Cuts at: 3350 3350
Size: 6127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3086 4252 5117 5415 3086
Size: 1166 865 298 3798
Fragments arranged by size:
3798 1166 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3275 4790 882
Size: 617 1776 1515 2219
Fragments arranged by size:
2219 1776 1515 617
BsaAI yAC'GTr
Cuts at: 493 1204 3022 3031 3321 3918 493
Size: 711 1818 9 290 597 2702
Fragments arranged by size:
2702 1818 711 597 290 9
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3934 4316 4951 275
Size: 53 83 186 936 2401 382 635 1451
Fragments arranged by size:
2401 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4560 4958 5232 5379 5790 4560
Size: 398 274 147 411 4897
Fragments arranged by size:
4897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5902
Fragments arranged by size:
5902 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2809 3341 1186
Size: 919 704 532 3972
Fragments arranged by size:
3972 919 704 532
BseYI C'CCAG_C
Cuts at: 2783 5330 5826 2783
Size: 2547 496 3084
Fragments arranged by size:
3084 2547 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2538 3071 1163
Size: 573 802 533 4219
Fragments arranged by size:
4219 802 573 533
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3577 4338 5366 1540
Size: 275 186 1576 761 1028 2301
Fragments arranged by size:
2301 1576 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4188 4273 5344 4188
Size: 85 1071 4971
Fragments arranged by size:
4971 1071 85
BsmI GAATG_Cn'
Cuts at: 2048 2538 3638 3731 2048
Size: 490 1100 93 4444
Fragments arranged by size:
4444 1100 490 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2351 2414 3050 3105 3243 3426 3446
Size: 399 561 63 636 55 138 183 20
Cuts at: 3446 4188 4273 5344 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 636 561 399 183 138
85 63 55 20
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 3204 4016 5746 3204
Size: 812 1730 3585
Fragments arranged by size:
3585 1730 812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2741 2848 3093 3482 844
Size: 1897 107 245 389 3489
Fragments arranged by size:
3489 1897 389 245 107
BsrBI CCG'CTC
Cuts at: 1394 1823 4014 1394
Size: 429 2191 3507
Fragments arranged by size:
3507 2191 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4622 4796 65
Size: 1183 3374 174 1396
Fragments arranged by size:
3374 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2868 4771 4958 1700
Size: 110 1058 1903 187 2869
Fragments arranged by size:
2869 1903 1058 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 3053 4187 5199 3053
Size: 1134 1012 3981
Fragments arranged by size:
3981 1134 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3382 794
Size: 2588 3539
BstBI TT'CG_AA
Cuts at: 4931 4931
Size: 6127
BstEII G'GTnAC_C
Cuts at: 1184 2356 1184
Size: 1172 4955
BstYI r'GATC_y
Cuts at: 1972 2285 2947 3379 3457 3824 4209 4226 5678
Size: 313 662 432 78 367 385 17 1452
Cuts at: 5678 5764 5776 6122 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 662 432 385 367 346 313
86 78 17 12
Bsu36I CC'TnA_GG
Cuts at: 2352 2352
Size: 6127
BtgI C'CryG_G
Cuts at: 513 1065 3170 513
Size: 552 2105 3470
Fragments arranged by size:
3470 2105 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5388
Fragments arranged by size:
5388 683 56
BtsI GCAGTG_nn'
Cuts at: 2714 3643 4437 4457 2714
Size: 929 794 20 4384
Fragments arranged by size:
4384 929 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2431 2466 529
Size: 35 1867 35 4190
Fragments arranged by size:
4190 1867 35 35
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3258 5134 809
Size: 507 1942 1876 1802
Fragments arranged by size:
1942 1876 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2721 3574 4463
Size: 54 1425 50 275 18 891 853 889
Cuts at: 4463 6092 8
Size: 1629 43
Fragments arranged by size:
1629 1425 891 889 853 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3574 1537
Size: 275 1762 4090
Fragments arranged by size:
4090 1762 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 2339 4057 1353
Size: 884 102 1718 3423
Fragments arranged by size:
3423 1718 884 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4714 5088 5234 387
Size: 1064 138 3125 374 146 1280
Fragments arranged by size:
3125 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2149 2280 2444 2528 2786 2906 3107
Size: 393 517 131 164 84 258 120 201
Cuts at: 3107 3167 3320 4190 4787 5574 5842 1239
Size: 60 153 870 597 787 268 1524
Fragments arranged by size:
1524 870 787 597 517 393 268 258
201 164 153 131 120 84 60
EcoNI CCTnn'n_nnAGG
Cuts at: 3089 3089
Size: 6127
EcoO109I rG'GnC_Cy
Cuts at: 2103 3061 3102 3239 3240 3251 3273 2103
Size: 958 41 137 1 11 22 4957
Fragments arranged by size:
4957 958 137 41 22 11 1
EcoRI G'AATT_C
Cuts at: 3436 3814 3436
Size: 378 5749
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 2635 5274 1678
Size: 957 2639 2531
Fragments arranged by size:
2639 2531 957
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2214 2246 4768 4800 4842
Size: 32 135 32 642 32 2522 32 42
Cuts at: 4842 4874 1373
Size: 32 2626
Fragments arranged by size:
2626 2522 642 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2686 3471 3717 669
Size: 988 281 748 785 246 3079
Fragments arranged by size:
3079 988 785 748 281 246
HindIII A'AGCT_T
Cuts at: 748 2454 3366 748
Size: 1706 912 3509
Fragments arranged by size:
3509 1706 912
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5241 5425 1305
Size: 3936 184 2007
Fragments arranged by size:
3936 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2723 10
Size: 54 1425 343 891 3414
Fragments arranged by size:
3414 1425 891 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2901 2919 3077 4085 4444 4603 518
Size: 648 1735 18 158 1008 359 159 2042
Fragments arranged by size:
2042 1735 1008 648 359 159 158 18
MspA1I CmG'CkG
Cuts at: 1606 2385 2662 2930 3543 4220 5368 5613 1606
Size: 779 277 268 613 677 1148 245 2120
Fragments arranged by size:
2120 1148 779 677 613 277 268 245
NaeI GCC'GGC
Cuts at: 2870 2870
Size: 6127
NcoI C'CATG_G
Cuts at: 513 1065 3170 513
Size: 552 2105 3470
Fragments arranged by size:
3470 2105 552
NdeI CA'TA_TG
Cuts at: 387 3397 387
Size: 3010 3117
NgoMIV G'CCGG_C
Cuts at: 2868 2868
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2099 2129 2834 3145 3485 5030 1132
Size: 97 870 30 705 311 340 1545 2229
Fragments arranged by size:
2229 1545 870 705 340 311 97 30
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6127
PciI A'CATG_T
Cuts at: 2125 5026 2125
Size: 2901 3226
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2027 3130 1108
Size: 919 1103 4105
Fragments arranged by size:
4105 1103 919
PfoI T'CCnGG_A
Cuts at: 2297 2297
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PmlI CAC'GTG
Cuts at: 3022 3022
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2103 3061 3251 2103
Size: 958 190 4979
Fragments arranged by size:
4979 958 190
PshAI GACnn'nnGTC
Cuts at: 1613 2114 2961 1613
Size: 501 847 4779
Fragments arranged by size:
4779 847 501
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PspOMI G'GGCC_C
Cuts at: 3101 3239 3101
Size: 138 5989
PstI C_TGCA'G
Cuts at: 830 2859 3291 3378 3479 830
Size: 2029 432 87 101 3478
Fragments arranged by size:
3478 2029 432 101 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 2385 2930 2385
Size: 545 5582
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SanDI GG'GwC_CC
Cuts at: 2103 3251 2103
Size: 1148 4979
SapI GCTCTTCn'nnn_
Cuts at: 2339 2339
Size: 6127
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SfcI C'TryA_G
Cuts at: 826 1045 2085 2386 2855 3287 3374 3475 4610
Size: 219 1040 301 469 432 87 101 1135
Cuts at: 4610 5291 5482 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1040 681 469 432 301 219
191 101 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3454 1872
Size: 1582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3065 4231 5132 5394 820
Size: 197 926 1122 1166 901 262 1553
Fragments arranged by size:
1553 1166 1122 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 3145 3485 1132
Size: 2013 340 3774
Fragments arranged by size:
3774 2013 340
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6127
StyI C'CwwG_G
Cuts at: 513 1065 2140 2622 3170 3566 513
Size: 552 1075 482 548 396 3074
Fragments arranged by size:
3074 1075 552 548 482 396
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2572 4154 4171 4324 4509 4954 325
Size: 1255 992 1582 17 153 185 445 1498
Fragments arranged by size:
1582 1498 1255 992 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2703 4373 6085
Size: 274 80 33 51 1443 726 1670 1712
Cuts at: 6085 96
Size: 138
Fragments arranged by size:
1712 1670 1443 726 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6087 6119 6087
Size: 32 6095
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 3452 1870
Size: 1582 4545
XmnI GAAnn'nnTTC
Cuts at: 2513 2654 4256 2513
Size: 141 1602 4384
Fragments arranged by size:
4384 1602 141
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PspXI PsrI
RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI
XcmI