Restriction map for
pFN21AE0948
(Circular) (Six-base) MAPSORT of: pFN21AE0948.seq Check: 5257 from: 1 to: 5983
pFN21AE0948 5983 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2106 3326 1937
Size: 169 1220 4594
Fragments arranged by size:
4594 1220 169
Acc65I G'GTAC_C
Cuts at: 3304 3304
Size: 5983
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 2819 3157 4046 5430 5698 1632
Size: 767 420 338 889 1384 268 1917
Fragments arranged by size:
1917 1384 889 767 420 338 268
AfeI AGC'GCT
Cuts at: 2870 2870
Size: 5983
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5786
AflIII A'CryG_T
Cuts at: 1203 4882 5859 1203
Size: 3679 977 1327
Fragments arranged by size:
3679 1327 977
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 2599 4712 2599
Size: 2113 3870
AleI CACnn'nnGTG
Cuts at: 1166 2528 1166
Size: 1362 4621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5983
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2397 2429 2397
Size: 32 5951
AlwNI CAG_nnn'CTG
Cuts at: 3076 3726 5298 3076
Size: 650 1572 3761
Fragments arranged by size:
3761 1572 650
ApaI G_GGCC'C
Cuts at: 2954 2954
Size: 5983
ApaLI G'TGCA_C
Cuts at: 3221 4040 5196 3221
Size: 819 1156 4008
Fragments arranged by size:
4008 1156 819
ApoI r'AATT_y
Cuts at: 1493 3292 3518 3670 5779 1493
Size: 1799 226 152 2109 1697
Fragments arranged by size:
2109 1799 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 2040 3308 1870
Size: 73 97 1268 4545
Fragments arranged by size:
4545 1268 97 73
BamHI G'GATC_C
Cuts at: 3313 3680 3313
Size: 367 5616
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2534 2551 3304 4759 618
Size: 325 217 627 747 17 753 1455 1842
Fragments arranged by size:
1842 1455 753 747 627 325 217 17
BanII G_rGCy'C
Cuts at: 1391 2061 2093 2796 2853 2954 3048 3111 3302
Size: 670 32 703 57 101 94 63 191
Cuts at: 3302 1391
Size: 4072
Fragments arranged by size:
4072 703 670 191 101 94 63 57
32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2837 3091 928
Size: 971 938 254 3820
Fragments arranged by size:
3820 971 938 254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4174 4208 1903
Size: 34 2237 34 3678
Fragments arranged by size:
3678 2237 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4594 136
Size: 107 122 71 631 605 2922 1525
Fragments arranged by size:
2922 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5978 5978
Size: 5983
BlpI GC'TnA_GC
Cuts at: 2308 3400 2308
Size: 1092 4891
Bme1580I G_kGCm'C
Cuts at: 1790 2537 2954 3225 4044 5200 1790
Size: 747 417 271 819 1156 2573
Fragments arranged by size:
2573 1156 819 747 417 271
BmgBI CAC'GTC
Cuts at: 1318 2464 1318
Size: 1146 4837
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3064 3714 4672
Size: 416 53 83 399 24 1647 650 958
Cuts at: 4672 442
Size: 1753
Fragments arranged by size:
1753 1647 958 650 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2360 2729 3173 3182 4643 1239
Size: 944 177 369 444 9 1461 2579
Fragments arranged by size:
2579 1461 944 444 369 177 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4108 4973 5271 4108
Size: 865 298 4820
Fragments arranged by size:
4820 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4646 882
Size: 617 3147 2219
Fragments arranged by size:
3147 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3774 493
Size: 711 2570 2702
Fragments arranged by size:
2702 2570 711
BsaBI GATnn'nnATC
Cuts at: 5977 5977
Size: 5983
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3790 4172 4807 275
Size: 53 83 186 936 2257 382 635 1451
Fragments arranged by size:
2257 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4416 4814 5088 5235 5646 4416
Size: 398 274 147 411 4753
Fragments arranged by size:
4753 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 3130 3160 1178
Size: 30 165 30 834 30 863 30 4001
Fragments arranged by size:
4001 863 834 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2180 2357 2909 3170 3200 1186
Size: 883 111 177 552 261 30 3969
Fragments arranged by size:
3969 883 552 261 177 111 30
BseYI C'CCAG_C
Cuts at: 5186 5682 5186
Size: 496 5487
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 3005 3260 1163
Size: 573 645 624 255 3886
Fragments arranged by size:
3886 645 624 573 255
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2077 2860 2984 3433 4194 5222
Size: 275 186 76 783 124 449 761 1028
Cuts at: 5222 1540
Size: 2301
Fragments arranged by size:
2301 1028 783 761 449 275 186 124
76
BsiHKAI G_wGCw'C
Cuts at: 2061 2270 2796 2853 3111 3225 4044 4129 5200
Size: 209 526 57 258 114 819 85 1071
Cuts at: 5200 2061
Size: 2844
Fragments arranged by size:
2844 1071 819 526 258 209 114 85
57
BsmI GAATG_Cn'
Cuts at: 3494 3587 3494
Size: 93 5890
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2093 2270 2537 2796 2853 2954
Size: 399 271 32 177 267 259 57 101
Cuts at: 2954 3048 3111 3225 3302 4044 4129 5200 1391
Size: 94 63 114 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 271 267 259 177
114 101 94 85 77 63 57 32
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 3872 5602 3872
Size: 1730 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3338 844
Size: 2494 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3132 3870 1394
Size: 429 1309 738 3507
Fragments arranged by size:
3507 1309 738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2352 4478 4652 65
Size: 1183 1104 2126 174 1396
Fragments arranged by size:
2126 1396 1183 1104 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4627 4814 1700
Size: 110 2817 187 2869
Fragments arranged by size:
2869 2817 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5983
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 4043 5055 4043
Size: 1012 4971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5983
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5983
BstXI CCAn_nnnn'nTGG
Cuts at: 2626 2626
Size: 5983
BstYI r'GATC_y
Cuts at: 1972 2412 3313 3680 4065 4082 5534 5620 5632
Size: 440 901 367 385 17 1452 86 12
Cuts at: 5632 5978 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 901 440 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2052 2556 2052
Size: 504 5479
BtgI C'CryG_G
Cuts at: 513 1065 2004 2781 513
Size: 552 939 777 3715
Fragments arranged by size:
3715 939 777 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5244
Fragments arranged by size:
5244 683 56
BtsI GCAGTG_nn'
Cuts at: 3499 4293 4313 3499
Size: 794 20 5169
Fragments arranged by size:
5169 794 20
ClaI AT'CG_AT
Cuts at: 1554 2118 1554
Size: 564 5419
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2118 2153 529
Size: 35 1554 35 4359
Fragments arranged by size:
4359 1554 35 35
DraI TTT'AAA
Cuts at: 3287 3287
Size: 5983
DraIII CAC_nnn'GTG
Cuts at: 3229 3229
Size: 5983
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2628 2784
Size: 54 1425 50 275 18 312 486 156
Cuts at: 2784 2981 3180 3430 4319 5948 8
Size: 197 199 250 889 1629 43
Fragments arranged by size:
1629 1425 889 486 312 275 250 199
197 156 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2981 3430 1537
Size: 275 1169 449 4090
Fragments arranged by size:
4090 1169 449 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 3913 1353
Size: 713 1847 3423
Fragments arranged by size:
3423 1847 713
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2489 2948 4570 4944 5090 387
Size: 1064 138 900 459 1622 374 146 1280
Fragments arranged by size:
1622 1280 1064 900 459 374 146 138
EcoICRI GAG'CTC
Cuts at: 2059 2794 2851 3109 2059
Size: 735 57 258 4933
Fragments arranged by size:
4933 735 258 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2360 2399 2729 2819 3157 3173
Size: 393 551 177 39 330 90 338 16
Cuts at: 3173 3182 4046 4643 5430 5698 1239
Size: 9 864 597 787 268 1524
Fragments arranged by size:
1524 864 787 597 551 393 338 330
268 177 90 39 16 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2165 3182 2165
Size: 1017 4966
EcoO109I rG'GnC_Cy
Cuts at: 2333 2383 2950 2951 3066 2333
Size: 50 567 1 115 5250
Fragments arranged by size:
5250 567 115 50 1
EcoRI G'AATT_C
Cuts at: 3292 3670 3292
Size: 378 5605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 5983
FspI TGC'GCA
Cuts at: 4489 4489
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 2872 5130 1678
Size: 1194 2258 2531
Fragments arranged by size:
2531 2258 1194
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2585 2617 4624 4656 4698
Size: 32 135 32 1013 32 2007 32 42
Cuts at: 4698 4730 1373
Size: 32 2626
Fragments arranged by size:
2626 2007 1013 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3327 3573 669
Size: 988 281 1389 246 3079
Fragments arranged by size:
3079 1389 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2999 748
Size: 2251 3732
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KpnI G_GTAC'C
Cuts at: 3308 3308
Size: 5983
MfeI C'AATT_G
Cuts at: 3582 3582
Size: 5983
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5097 5281 1305
Size: 3792 184 2007
Fragments arranged by size:
3792 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 2630 10
Size: 54 1425 343 312 486 3363
Fragments arranged by size:
3363 1425 486 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 3941 4300 4459 518
Size: 648 1362 1413 359 159 2042
Fragments arranged by size:
2042 1413 1362 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3399 4076 5224 5469 1606
Size: 1793 677 1148 245 2120
Fragments arranged by size:
2120 1793 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4492
Fragments arranged by size:
4492 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 3341 4886 1132
Size: 97 2112 1545 2229
Fragments arranged by size:
2229 2112 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2605 1348
Size: 1257 4726
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2277 1108
Size: 1169 4814
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4052 4084 5565 5597 4052
Size: 32 1481 32 4438
Fragments arranged by size:
4438 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2333 3066 2333
Size: 733 5250
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PspOMI G'GGCC_C
Cuts at: 2950 2950
Size: 5983
PstI C_TGCA'G
Cuts at: 830 3335 830
Size: 2505 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
SacI G_AGCT'C
Cuts at: 2061 2796 2853 3111 2061
Size: 735 57 258 4933
Fragments arranged by size:
4933 735 258 57
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SapI GCTCTTCn'nnn_
Cuts at: 2066 2066
Size: 5983
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SexAI A'CCwGG_T
Cuts at: 2329 3225 2329
Size: 896 5087
SfcI C'TryA_G
Cuts at: 826 1045 3331 4466 5147 5338 826
Size: 219 2286 1135 681 191 1471
Fragments arranged by size:
2286 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5983
SmaI CCC'GGG
Cuts at: 1872 3310 1872
Size: 1438 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2040 4087 4988 5250 820
Size: 197 926 97 2047 901 262 1553
Fragments arranged by size:
2047 1553 926 901 262 197 97
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 3341 1132
Size: 2209 3774
SspI AAT'ATT
Cuts at: 5 52 3907 5
Size: 47 3855 2081
Fragments arranged by size:
3855 2081 47
StuI AGG'CCT
Cuts at: 1517 2750 1517
Size: 1233 4750
StyI C'CwwG_G
Cuts at: 513 1065 2004 2631 2787 3422 513
Size: 552 939 627 156 635 3074
Fragments arranged by size:
3074 939 635 627 552 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2716 3134 4010 4027 4180 4365 4810
Size: 1255 1136 418 876 17 153 185 445
Cuts at: 4810 325
Size: 1498
Fragments arranged by size:
1498 1255 1136 876 445 418 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4229 5941 96
Size: 274 80 33 51 1443 2252 1712 138
Fragments arranged by size:
2252 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3130 3162 3257 3289 5943 5975 3130
Size: 32 95 32 2654 32 3138
Fragments arranged by size:
3138 2654 95 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5983
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XhoI C'TCGA_G
Cuts at: 1943 2040 1943
Size: 97 5886
XmaI C'CCGG_G
Cuts at: 1870 3308 1870
Size: 1438 4545
XmnI GAAnn'nnTTC
Cuts at: 2525 3170 4112 2525
Size: 645 942 4396
Fragments arranged by size:
4396 942 645
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI
SwaI XcmI