Restriction map for
pFN21AE0947
(Circular) (Six-base) MAPSORT of: pFN21AE0947.seq Check: 9942 from: 1 to: 5860
pFN21AE0947 5860 bp
With 182 enzymes: SgfI *
November 18, 2016 17:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3203 1937
Size: 1266 4594
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3923 5307 5575 1632
Size: 2291 1384 268 1917
Fragments arranged by size:
2291 1917 1384 268
AfeI AGC'GCT
Cuts at: 2049 2049
Size: 5860
AflII C'TTAA_G
Cuts at: 820 1017 3124 820
Size: 197 2107 3556
Fragments arranged by size:
3556 2107 197
AflIII A'CryG_T
Cuts at: 1203 2736 4759 5736 1203
Size: 1533 2023 977 1327
Fragments arranged by size:
2023 1533 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AlwNI CAG_nnn'CTG
Cuts at: 2947 3603 5175 2947
Size: 656 1572 3632
Fragments arranged by size:
3632 1572 656
ApaI G_GGCC'C
Cuts at: 2112 2112
Size: 5860
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 2601 3169 3395 3547 5656 1493
Size: 1108 568 226 152 2109 1697
Fragments arranged by size:
2109 1697 1108 568 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 2111 2175 3185 1870
Size: 73 92 76 64 1010 4545
Fragments arranged by size:
4545 1010 92 76 73 64
BamHI G'GATC_C
Cuts at: 2462 2490 3190 3557 2462
Size: 28 700 367 4765
Fragments arranged by size:
4765 700 367 28
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2015 3181 4636 618
Size: 325 217 627 228 1166 1455 1842
Fragments arranged by size:
1842 1455 1166 627 325 228 217
BanII G_rGCy'C
Cuts at: 1391 2112 2439 2746 3179 1391
Size: 721 327 307 433 4072
Fragments arranged by size:
4072 721 433 327 307
BbeI G_GCGC'C
Cuts at: 2019 2019
Size: 5860
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2258 2669 2976 3063 928
Size: 971 359 411 307 87 3725
Fragments arranged by size:
3725 971 411 359 307 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4051 4085 1903
Size: 34 2114 34 3678
Fragments arranged by size:
3678 2114 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2133 3752 4962 2133
Size: 1619 1210 3031
Fragments arranged by size:
3031 1619 1210
BclI T'GATC_A
Cuts at: 1548 2754 2964 1548
Size: 1206 210 4444
Fragments arranged by size:
4444 1206 210
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2129 4471 136
Size: 107 122 71 631 605 457 2342 1525
Fragments arranged by size:
2342 1525 631 605 457 122 107 71
BglII A'GATC_T
Cuts at: 2379 5855 2379
Size: 3476 2384
BlpI GC'TnA_GC
Cuts at: 2330 3277 2330
Size: 947 4913
Bme1580I G_kGCm'C
Cuts at: 1790 2112 3921 5077 1790
Size: 322 1809 1156 2573
Fragments arranged by size:
2573 1809 1156 322
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3591 4549 442
Size: 416 53 83 399 24 2174 958 1753
Fragments arranged by size:
2174 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2453 3054 2453
Size: 601 5259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2480 2984 4520 1239
Size: 1241 504 1536 2579
Fragments arranged by size:
2579 1536 1241 504
Bpu10I CC'TnA_GC
Cuts at: 2457 2708 2457
Size: 251 5609
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3985 4850 5148 3985
Size: 865 298 4697
Fragments arranged by size:
4697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4523 882
Size: 617 3024 2219
Fragments arranged by size:
3024 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3651 493
Size: 711 2447 2702
Fragments arranged by size:
2702 2447 711
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2016 2088 3667 4049
Size: 53 83 186 936 483 72 1579 382
Cuts at: 4049 4684 275
Size: 635 1451
Fragments arranged by size:
1579 1451 936 635 483 382 186 83
72 53
BsaWI w'CCGG_w
Cuts at: 2426 4293 4691 4965 5112 5523 2426
Size: 1867 398 274 147 411 2763
Fragments arranged by size:
2763 1867 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5635
Fragments arranged by size:
5635 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5860
BseYI C'CCAG_C
Cuts at: 5063 5559 5063
Size: 496 5364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2748 3048 1163
Size: 573 1012 300 3975
Fragments arranged by size:
3975 1012 573 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2439 3921 4006 5077 2439
Size: 1482 85 1071 3222
Fragments arranged by size:
3222 1482 1071 85
BsmI GAATG_Cn'
Cuts at: 3371 3464 3371
Size: 93 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2439 2746 3179 3921 4006 5077
Size: 399 322 327 307 433 742 85 1071
Cuts at: 5077 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 433 399 327 322 307
85
BspEI T'CCGG_A
Cuts at: 5523 5523
Size: 5860
BspHI T'CATG_A
Cuts at: 3749 5479 3749
Size: 1730 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3215 844
Size: 2371 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2476 3747 1394
Size: 429 653 1271 3507
Fragments arranged by size:
3507 1271 653 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2451 4355 4529 65
Size: 1183 1203 1904 174 1396
Fragments arranged by size:
1904 1396 1203 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2084 4504 4691 1700
Size: 110 274 2420 187 2869
Fragments arranged by size:
2869 2420 274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 3920 4932 3920
Size: 1012 4848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2533 794
Size: 1739 4121
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5860
BstXI CCAn_nnnn'nTGG
Cuts at: 2731 2731
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 2379 2462 2490 3059 3190 3557 3942 3959
Size: 407 83 28 569 131 367 385 17
Cuts at: 3959 5411 5497 5509 5855 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 569 407 385 367 346 131
86 83 28 17 12
Bsu36I CC'TnA_GG
Cuts at: 2697 2697
Size: 5860
BtgI C'CryG_G
Cuts at: 513 1065 2004 2970 513
Size: 552 939 966 3403
Fragments arranged by size:
3403 966 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2909 487
Size: 683 56 1683 3438
Fragments arranged by size:
3438 1683 683 56
BtsI GCAGTG_nn'
Cuts at: 2736 3376 4170 4190 2736
Size: 640 794 20 4406
Fragments arranged by size:
4406 794 640 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DraIII CAC_nnn'GTG
Cuts at: 2672 2672
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2177 4867 809
Size: 507 861 2690 1802
Fragments arranged by size:
2690 1802 861 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2861 2938 3307
Size: 54 1425 50 275 18 1031 77 369
Cuts at: 3307 4196 5825 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1031 889 369 275 77 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2586 3790 1353
Size: 1233 1204 3423
Fragments arranged by size:
3423 1233 1204
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4447 4821 4967 387
Size: 1064 138 2858 374 146 1280
Fragments arranged by size:
2858 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2437 2437
Size: 5860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2480 2984 3923 4520 5307 5575 1239
Size: 393 848 504 939 597 787 268 1524
Fragments arranged by size:
1524 939 848 787 597 504 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2102 2179 2676 2944 3033 2102
Size: 77 497 268 89 4929
Fragments arranged by size:
4929 497 268 89 77
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2733 3139 2733
Size: 406 5454
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 2019 2051 5007 1678
Size: 341 32 2956 2531
Fragments arranged by size:
2956 2531 341 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2181 2213 2380 2412 4501
Size: 32 135 32 609 32 167 32 2089
Cuts at: 4501 4533 4575 4607 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2089 609 167 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KasI G'GCGC_C
Cuts at: 2015 2015
Size: 5860
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2071 4974 5158 1305
Size: 766 2903 184 2007
Fragments arranged by size:
2903 2007 766 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2863 2940 10
Size: 54 1425 343 1031 77 2930
Fragments arranged by size:
2930 1425 1031 343 77 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2411 2586 3818 4177 4336 518
Size: 648 1245 175 1232 359 159 2042
Fragments arranged by size:
2042 1245 1232 648 359 175 159
MspA1I CmG'CkG
Cuts at: 1606 2936 3276 3953 5101 5346 1606
Size: 1330 340 677 1148 245 2120
Fragments arranged by size:
2120 1330 1148 677 340 245
NaeI GCC'GGC
Cuts at: 2086 2086
Size: 5860
NarI GG'CG_CC
Cuts at: 2016 2016
Size: 5860
NcoI C'CATG_G
Cuts at: 513 1065 2004 2970 513
Size: 552 939 966 3403
Fragments arranged by size:
3403 966 939 552
NdeI CA'TA_TG
Cuts at: 387 2761 3105 387
Size: 2374 344 3142
Fragments arranged by size:
3142 2374 344
NgoMIV G'CCGG_C
Cuts at: 2084 2084
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 2531 2740 2932 3218 4763 1132
Size: 97 1302 209 192 286 1545 2229
Fragments arranged by size:
2229 1545 1302 286 209 192 97
PasI CC'CwG_GG
Cuts at: 1348 2958 1348
Size: 1610 4250
PciI A'CATG_T
Cuts at: 2736 4759 2736
Size: 2023 3837
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2374 2947 1108
Size: 1266 573 4021
Fragments arranged by size:
4021 1266 573
PfoI T'CCnGG_A
Cuts at: 2633 3055 2633
Size: 422 5438
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2179 2676 2179
Size: 497 5363
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PspOMI G'GGCC_C
Cuts at: 2108 2108
Size: 5860
PstI C_TGCA'G
Cuts at: 830 2538 2980 3212 830
Size: 1708 442 232 3478
Fragments arranged by size:
3478 1708 442 232
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2936 2936
Size: 5860
SacI G_AGCT'C
Cuts at: 2439 2439
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SanDI GG'GwC_CC
Cuts at: 2676 2676
Size: 5860
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 2534 2976 3208 4343 5024 5215 826
Size: 219 1489 442 232 1135 681 191 1471
Fragments arranged by size:
1489 1471 1135 681 442 232 219 191
SfoI GGC'GCC
Cuts at: 2017 2017
Size: 5860
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 2037 2177 3187 1872
Size: 165 140 1010 4545
Fragments arranged by size:
4545 1010 165 140
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3124 3964 4865 5127 820
Size: 197 926 1181 840 901 262 1553
Fragments arranged by size:
1553 1181 926 901 840 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 2531 3218 1132
Size: 1399 687 3774
Fragments arranged by size:
3774 1399 687
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 2004 2858 2970 3299 513
Size: 552 939 854 112 329 3074
Fragments arranged by size:
3074 939 854 552 329 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2950 3887 3904 4057 4242 4687 325
Size: 1255 1370 937 17 153 185 445 1498
Fragments arranged by size:
1498 1370 1255 937 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2685 4106 5818
Size: 274 80 33 51 1443 708 1421 1712
Cuts at: 5818 96
Size: 138
Fragments arranged by size:
1712 1443 1421 708 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2629 2661 5820 5852 2629
Size: 32 3159 32 2637
Fragments arranged by size:
3159 2637 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2026 2052 2026
Size: 26 5834
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 2035 2175 3185 1870
Size: 165 140 1010 4545
Fragments arranged by size:
4545 1010 165 140
XmnI GAAnn'nnTTC
Cuts at: 3989 3989
Size: 5860
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SapI SexAI
SfiI SrfI SwaI