Restriction map for
pFN21AE0945
(Circular) (Six-base) MAPSORT of: pFN21AE0945.seq Check: 2818 from: 1 to: 5755
pFN21AE0945 5755 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2167 2276 2650 3818 5202 5470 1632
Size: 535 109 374 1168 1384 268 1917
Fragments arranged by size:
1917 1384 1168 535 374 268 109
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 2401 2523 4654 5631 1203
Size: 1198 122 2131 977 1327
Fragments arranged by size:
2131 1327 1198 977 122
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2334 1184
Size: 1150 4605
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2699 2731 2699
Size: 32 5723
AlwNI CAG_nnn'CTG
Cuts at: 2380 2650 3498 5070 2380
Size: 270 848 1572 3065
Fragments arranged by size:
3065 1572 848 270
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 3064 3290 3442 5551 1493
Size: 1571 226 152 2109 1697
Fragments arranged by size:
2109 1697 1571 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2571 4309 160
Size: 872 1539 1738 1606
Fragments arranged by size:
1738 1606 1539 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 3080 1870
Size: 73 103 1034 4545
Fragments arranged by size:
4545 1034 103 73
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 3076 4531 618
Size: 325 217 627 266 1023 1455 1842
Fragments arranged by size:
1842 1455 1023 627 325 266 217
BanII G_rGCy'C
Cuts at: 1391 3074 1391
Size: 1683 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2639 928
Size: 971 740 4044
Fragments arranged by size:
4044 971 740
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3946 3980 1903
Size: 34 2009 34 3678
Fragments arranged by size:
3678 2009 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3004 3647 4857 3004
Size: 643 1210 3902
Fragments arranged by size:
3902 1210 643
BclI T'GATC_A
Cuts at: 1548 2079 1548
Size: 531 5224
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2595 4366 136
Size: 107 122 71 631 605 923 1771 1525
Fragments arranged by size:
1771 1525 923 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 2600 2889 3816 4972 1790
Size: 810 289 927 1156 2573
Fragments arranged by size:
2573 1156 927 810 289
BmgBI CAC'GTC
Cuts at: 1318 2800 1318
Size: 1482 4273
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2638 3486 4444
Size: 416 53 83 399 24 1221 848 958
Cuts at: 4444 442
Size: 1753
Fragments arranged by size:
1753 1221 958 848 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2970 2970
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2561 2754 2854 3008 4415 1239
Size: 1124 198 193 100 154 1407 2579
Fragments arranged by size:
2579 1407 1124 198 193 154 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2494 3880 4745 5043 2494
Size: 1386 865 298 3206
Fragments arranged by size:
3206 1386 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2984 4418 882
Size: 617 1485 1434 2219
Fragments arranged by size:
2219 1485 1434 617
BsaAI yAC'GTr
Cuts at: 493 1204 2526 3546 493
Size: 711 1322 1020 2702
Fragments arranged by size:
2702 1322 1020 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2691 3562 3944 4579
Size: 53 83 186 936 1158 871 382 635
Cuts at: 4579 275
Size: 1451
Fragments arranged by size:
1451 1158 936 871 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2699 2729 2784 2814 1178
Size: 30 165 30 1296 30 55 30 4119
Fragments arranged by size:
4119 1296 165 55 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2127 2243 1186
Size: 941 116 4698
Fragments arranged by size:
4698 941 116
BseYI C'CCAG_C
Cuts at: 4958 5454 4958
Size: 496 5259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2564 1163
Size: 573 828 4354
Fragments arranged by size:
4354 828 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2313 3816 3901 4972 2313
Size: 1503 85 1071 3096
Fragments arranged by size:
3096 1503 1071 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2600 2889 3074 3816 3901 4972
Size: 399 523 287 289 185 742 85 1071
Cuts at: 4972 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 523 399 289 287 185
85
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2586 2634 3110 844
Size: 1742 48 476 3489
Fragments arranged by size:
3489 1742 476 48
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4250 4424 65
Size: 1183 3002 174 1396
Fragments arranged by size:
3002 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4399 4586 1700
Size: 110 2589 187 2869
Fragments arranged by size:
2869 2589 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 2856 3085 3452 3837 3854 5306 5392 5404
Size: 884 229 367 385 17 1452 86 12
Cuts at: 5404 5750 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 884 385 367 346 229 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2365 2550 513
Size: 552 939 361 185 3718
Fragments arranged by size:
3718 939 552 361 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2116 487
Size: 683 56 890 4126
Fragments arranged by size:
4126 890 683 56
BtsI GCAGTG_nn'
Cuts at: 2970 3271 4065 4085 2970
Size: 301 794 20 4640
Fragments arranged by size:
4640 794 301 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2547 2737 3202
Size: 54 1425 50 275 18 717 190 465
Cuts at: 3202 4091 5720 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 717 465 275 190 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3685 1353
Size: 2332 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2167 2276 2363 2561 2650 2754 2854
Size: 393 535 109 87 198 89 104 100
Cuts at: 2854 3008 3818 4415 5202 5470 1239
Size: 154 810 597 787 268 1524
Fragments arranged by size:
1524 810 787 597 535 393 268 198
154 109 104 100 89 87
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 5755
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 4902 1678
Size: 3224 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2083 2115 4396 4428 4470
Size: 32 135 32 511 32 2281 32 42
Cuts at: 4470 4502 1373
Size: 32 2626
Fragments arranged by size:
2626 2281 511 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2643 4869 5053 1305
Size: 1338 2226 184 2007
Fragments arranged by size:
2226 2007 1338 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2549 10
Size: 54 1425 343 717 3216
Fragments arranged by size:
3216 1425 717 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3713 4072 4231 518
Size: 648 2547 359 159 2042
Fragments arranged by size:
2547 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2254 3171 3848 4996 5241 1606
Size: 457 191 917 677 1148 245 2120
Fragments arranged by size:
2120 1148 917 677 457 245 191
NcoI C'CATG_G
Cuts at: 513 1065 2004 2365 2550 513
Size: 552 939 361 185 3718
Fragments arranged by size:
3718 939 552 361 185
NdeI CA'TA_TG
Cuts at: 387 2830 387
Size: 2443 3312
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2405 2943 3113 4658 1132
Size: 97 1176 538 170 1545 2229
Fragments arranged by size:
2229 1545 1176 538 170 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 2401 4654 2401
Size: 2253 3502
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2066 1108
Size: 958 4797
PfoI T'CCnGG_A
Cuts at: 2717 2717
Size: 5755
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PmlI CAC'GTG
Cuts at: 2526 2526
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2699 2731 3824 3856 5337 5369 2699
Size: 32 1093 32 1481 32 3085
Fragments arranged by size:
3085 1481 1093 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PstI C_TGCA'G
Cuts at: 830 2376 3107 830
Size: 1546 731 3478
Fragments arranged by size:
3478 1546 731
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
PvuII CAG'CTG
Cuts at: 2063 2254 2063
Size: 191 5564
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2372 3103 4238 4919 5110 826
Size: 219 1327 731 1135 681 191 1471
Fragments arranged by size:
1471 1327 1135 731 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2473 3859 4760 5022 820
Size: 197 926 530 1386 901 262 1553
Fragments arranged by size:
1553 1386 926 901 530 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 2943 3113 1132
Size: 1811 170 3774
Fragments arranged by size:
3774 1811 170
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 2037 1517
Size: 520 5235
StyI C'CwwG_G
Cuts at: 513 1065 2004 2213 2365 2392 2550 3194 513
Size: 552 939 209 152 27 158 644 3074
Fragments arranged by size:
3074 939 644 552 209 158 152 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3782 3799 3952 4137 4582 325
Size: 1255 2202 17 153 185 445 1498
Fragments arranged by size:
2202 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2152 2184 2784 2816 5715 5747 2152
Size: 32 600 32 2899 32 2160
Fragments arranged by size:
2899 2160 600 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2558 2638 1316
Size: 1242 80 4433
Fragments arranged by size:
4433 1242 80
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2778 2878 2778
Size: 100 5655
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI