Restriction map for
pFN21AE0942
(Circular) (Six-base) MAPSORT of: pFN21AE0942.seq Check: 2221 from: 1 to: 5587
pFN21AE0942 5587 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3397 278
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2930 1937
Size: 993 4594
Acc65I G'GTAC_C
Cuts at: 2908 2908
Size: 5587
AclI AA'CG_TT
Cuts at: 1563 3714 4087 1563
Size: 2151 373 3063
Fragments arranged by size:
3063 2151 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3650 5034 5302 1632
Size: 2018 1384 268 1917
Fragments arranged by size:
2018 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5390
AflIII A'CryG_T
Cuts at: 1203 2305 4486 5463 1203
Size: 1102 2181 977 1327
Fragments arranged by size:
2181 1327 1102 977
AgeI A'CCGG_T
Cuts at: 4418 4418
Size: 5587
AhdI GACnn_n'nnGTC
Cuts at: 4316 4316
Size: 5587
AleI CACnn'nnGTG
Cuts at: 1166 2870 1166
Size: 1704 3883
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5587
AlwNI CAG_nnn'CTG
Cuts at: 2140 2599 3330 4902 2140
Size: 459 731 1572 2825
Fragments arranged by size:
2825 1572 731 459
ApaLI G'TGCA_C
Cuts at: 3644 4800 3644
Size: 1156 4431
ApoI r'AATT_y
Cuts at: 1493 2896 3122 3274 5383 1493
Size: 1403 226 152 2109 1697
Fragments arranged by size:
2109 1697 1403 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4141 160
Size: 872 3109 1606
Fragments arranged by size:
3109 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
AvaI C'yCGr_G
Cuts at: 1870 1943 2348 2912 1870
Size: 73 405 564 4545
Fragments arranged by size:
4545 564 405 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2623 2656 2623
Size: 33 5554
BamHI G'GATC_C
Cuts at: 2917 3284 2917
Size: 367 5220
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2028 2114 2908 4363 618
Size: 325 217 627 241 86 794 1455 1842
Fragments arranged by size:
1842 1455 794 627 325 241 217 86
BanII G_rGCy'C
Cuts at: 1391 2225 2280 2529 2906 1391
Size: 834 55 249 377 4072
Fragments arranged by size:
4072 834 377 249 55
BbeI G_GCGC'C
Cuts at: 2118 2118
Size: 5587
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2673 928
Size: 971 774 3842
Fragments arranged by size:
3842 971 774
BbvCI CC'TCA_GC
Cuts at: 2730 2730
Size: 5587
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3778 3812 1903
Size: 34 1841 34 3678
Fragments arranged by size:
3678 1841 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2651 3479 4689 2651
Size: 828 1210 3549
Fragments arranged by size:
3549 1210 828
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5587
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4198 136
Size: 107 122 71 631 605 2526 1525
Fragments arranged by size:
2526 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5582 5582
Size: 5587
BlpI GC'TnA_GC
Cuts at: 2383 3004 2383
Size: 621 4966
Bme1580I G_kGCm'C
Cuts at: 1790 2476 3648 4804 1790
Size: 686 1172 1156 2573
Fragments arranged by size:
2573 1172 1156 686
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5587
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2228 2755 3318
Size: 416 53 83 399 24 811 527 563
Cuts at: 3318 4276 442
Size: 958 1753
Fragments arranged by size:
1753 958 811 563 527 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2582 4247 1239
Size: 797 546 1665 2579
Fragments arranged by size:
2579 1665 797 546
Bpu10I CC'TnA_GC
Cuts at: 2177 2730 2177
Size: 553 5034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3712 4577 4875 3712
Size: 865 298 4424
Fragments arranged by size:
4424 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2540 4250 882
Size: 617 1041 1710 2219
Fragments arranged by size:
2219 1710 1041 617
BsaAI yAC'GTr
Cuts at: 493 1204 2306 3378 493
Size: 711 1102 1072 2702
Fragments arranged by size:
2702 1102 1072 711
BsaBI GATnn'nnATC
Cuts at: 5581 5581
Size: 5587
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 2318 3394 3776
Size: 53 83 186 936 582 203 1076 382
Cuts at: 3776 4411 275
Size: 635 1451
Fragments arranged by size:
1451 1076 936 635 582 382 203 186
83 53
BsaWI w'CCGG_w
Cuts at: 2583 4020 4418 4692 4839 5250 2583
Size: 1437 398 274 147 411 2920
Fragments arranged by size:
2920 1437 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2306 2336 1178
Size: 30 165 30 903 30 4429
Fragments arranged by size:
4429 903 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 2327 1186
Size: 997 144 4446
Fragments arranged by size:
4446 997 144
BseYI C'CCAG_C
Cuts at: 2110 2556 4790 5286 2110
Size: 446 2234 496 2411
Fragments arranged by size:
2411 2234 496 446
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2346 2558 1163
Size: 573 610 212 4192
Fragments arranged by size:
4192 610 573 212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3037 3798 4826 1540
Size: 275 186 1036 761 1028 2301
Fragments arranged by size:
2301 1036 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2280 2529 3648 3733 4804 2280
Size: 249 1119 85 1071 3063
Fragments arranged by size:
3063 1119 1071 249 85
BsmI GAATG_Cn'
Cuts at: 3098 3191 3098
Size: 93 5494
BsmBI CGTCTCn'nnnn_
Cuts at: 2309 2309
Size: 5587
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2225 2280 2476 2529 2906 3648 3733
Size: 399 435 55 196 53 377 742 85
Cuts at: 3733 4804 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 435 399 377 196 85
55 53
BspEI T'CCGG_A
Cuts at: 5250 5250
Size: 5587
BspHI T'CATG_A
Cuts at: 3476 5206 3476
Size: 1730 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2942 844
Size: 2098 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2020 2589 3474 1394
Size: 429 197 569 885 3507
Fragments arranged by size:
3507 885 569 429 197
BsrDI GCAATG_nn'
Cuts at: 65 1248 4082 4256 65
Size: 1183 2834 174 1396
Fragments arranged by size:
2834 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4231 4418 1700
Size: 110 2421 187 2869
Fragments arranged by size:
2869 2421 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5587
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5587
BssSI C'ACGA_G
Cuts at: 3647 4659 3647
Size: 1012 4575
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2260 2340 2599 794
Size: 1466 80 259 3782
Fragments arranged by size:
3782 1466 259 80
BstBI TT'CG_AA
Cuts at: 4391 4391
Size: 5587
BstEII G'GTnAC_C
Cuts at: 1184 2779 1184
Size: 1595 3992
BstYI r'GATC_y
Cuts at: 1972 2917 3284 3669 3686 5138 5224 5236 5582
Size: 945 367 385 17 1452 86 12 346
Cuts at: 5582 1972
Size: 1977
Fragments arranged by size:
1977 1452 945 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2610 2695 513
Size: 552 939 606 85 3405
Fragments arranged by size:
3405 939 606 552 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4848
Fragments arranged by size:
4848 683 56
BtsI GCAGTG_nn'
Cuts at: 2483 3103 3897 3917 2483
Size: 620 794 20 4153
Fragments arranged by size:
4153 794 620 20
ClaI AT'CG_AT
Cuts at: 1554 2161 1554
Size: 607 4980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5552
DraI TTT'AAA
Cuts at: 2891 2891
Size: 5587
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2507 4594 809
Size: 507 1191 2087 1802
Fragments arranged by size:
2087 1802 1191 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 2681 3034
Size: 54 1425 50 275 18 528 323 353
Cuts at: 3034 3923 5552 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 528 353 323 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3034 1537
Size: 275 1222 4090
Fragments arranged by size:
4090 1222 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3517 1353
Size: 2164 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2234 4174 4548 4694 387
Size: 1064 138 645 1940 374 146 1280
Fragments arranged by size:
1940 1280 1064 645 374 146 138
EcoICRI GAG'CTC
Cuts at: 2278 2527 2278
Size: 249 5338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2582 3650 4247 5034 5302 1239
Size: 393 404 546 1068 597 787 268 1524
Fragments arranged by size:
1524 1068 787 597 546 404 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2729 2729
Size: 5587
EcoRI G'AATT_C
Cuts at: 2896 3274 2896
Size: 378 5209
FspI TGC'GCA
Cuts at: 4093 4093
Size: 5587
HaeII r_GCGC'y
Cuts at: 1678 2118 4734 1678
Size: 440 2616 2531
Fragments arranged by size:
2616 2531 440
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2154 2186 4228 4260 4302
Size: 32 135 32 582 32 2042 32 42
Cuts at: 4302 4334 1373
Size: 32 2626
Fragments arranged by size:
2626 2042 582 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2512 2931 3177 669
Size: 988 281 574 419 246 3079
Fragments arranged by size:
3079 988 574 419 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5587
HpaI GTT'AAC
Cuts at: 3177 3177
Size: 5587
KasI G'GCGC_C
Cuts at: 2114 2114
Size: 5587
KpnI G_GTAC'C
Cuts at: 2912 2912
Size: 5587
MfeI C'AATT_G
Cuts at: 3186 3186
Size: 5587
MluI A'CGCG_T
Cuts at: 5463 5463
Size: 5587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4701 4885 1305
Size: 3396 184 2007
Fragments arranged by size:
3396 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2683 10
Size: 54 1425 343 851 2914
Fragments arranged by size:
2914 1425 851 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2870 3545 3904 4063 518
Size: 648 1704 675 359 159 2042
Fragments arranged by size:
2042 1704 675 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2560 2676 2812 3003 3680 4828 5073
Size: 531 423 116 136 191 677 1148 245
Cuts at: 5073 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 531 423 245 191 136
116
NarI GG'CG_CC
Cuts at: 2115 2115
Size: 5587
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4096
Fragments arranged by size:
4096 939 552
NdeI CA'TA_TG
Cuts at: 387 2869 387
Size: 2482 3105
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5587
NotI GC'GGCC_GC
Cuts at: 3034 3034
Size: 5587
NspI r_CATG'y
Cuts at: 1132 1229 2945 4490 1132
Size: 97 1716 1545 2229
Fragments arranged by size:
2229 1716 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2389 1348
Size: 1041 4546
PciI A'CATG_T
Cuts at: 4486 4486
Size: 5587
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2203 1108
Size: 1095 4492
PmeI GTTT'AAAC
Cuts at: 2891 2891
Size: 5587
PmlI CAC'GTG
Cuts at: 2306 2306
Size: 5587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 5169 5201 3656
Size: 32 1481 32 4042
Fragments arranged by size:
4042 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5587
PsiI TTA'TAA
Cuts at: 3157 3157
Size: 5587
PspXI vC'TCGA_Gb
Cuts at: 2348 2348
Size: 5587
PstI C_TGCA'G
Cuts at: 830 2046 2604 2939 830
Size: 1216 558 335 3478
Fragments arranged by size:
3478 1216 558 335
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5587
PvuII CAG'CTG
Cuts at: 2137 2560 2812 2137
Size: 423 252 4912
Fragments arranged by size:
4912 423 252
SacI G_AGCT'C
Cuts at: 2280 2529 2280
Size: 249 5338
SalI G'TCGA_C
Cuts at: 1936 2929 1936
Size: 993 4594
SbfI CC_TGCA'GG
Cuts at: 2939 2939
Size: 5587
ScaI AGT'ACT
Cuts at: 3835 5547 3835
Size: 1712 3875
SfcI C'TryA_G
Cuts at: 826 1045 2042 2121 2437 2600 2935 4070 4751
Size: 219 997 79 316 163 335 1135 681
Cuts at: 4751 4942 826
Size: 191 1471
Fragments arranged by size:
1471 1135 997 681 335 316 219 191
163 79
SfoI GGC'GCC
Cuts at: 2116 2116
Size: 5587
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5587
SmaI CCC'GGG
Cuts at: 1872 2914 1872
Size: 1042 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2348 3691 4592 4854 820
Size: 197 926 405 1343 901 262 1553
Fragments arranged by size:
1553 1343 926 901 405 262 197
SnaBI TAC'GTA
Cuts at: 493 3378 493
Size: 2885 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5587
SphI G_CATG'C
Cuts at: 1132 2945 1132
Size: 1813 3774
SspI AAT'ATT
Cuts at: 5 52 3511 5
Size: 47 3459 2081
Fragments arranged by size:
3459 2081 47
StuI AGG'CCT
Cuts at: 1517 2879 1517
Size: 1362 4225
StyI C'CwwG_G
Cuts at: 513 1065 2004 3026 513
Size: 552 939 1022 3074
Fragments arranged by size:
3074 1022 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3614 3631 3784 3969 4414 325
Size: 1255 2034 17 153 185 445 1498
Fragments arranged by size:
2034 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3833 5545 96
Size: 274 80 33 51 1443 1856 1712 138
Fragments arranged by size:
1856 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2306 2338 5547 5579 2306
Size: 32 3209 32 2314
Fragments arranged by size:
3209 2314 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5587
XbaI T'CTAG_A
Cuts at: 2923 2923
Size: 5587
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2107 2011
Size: 96 5491
XhoI C'TCGA_G
Cuts at: 1943 2348 1943
Size: 405 5182
XmaI C'CCGG_G
Cuts at: 1870 2912 1870
Size: 1042 4545
XmnI GAAnn'nnTTC
Cuts at: 3716 3716
Size: 5587
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3395 276
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PpuMI PspOMI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI