Restriction map for
pFN21AE0940
(Circular) (Six-base) MAPSORT of: pFN21AE0940.seq Check: 6701 from: 1 to: 6082
pFN21AE0940 6082 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3892 278
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3425 1937
Size: 1488 4594
Acc65I G'GTAC_C
Cuts at: 3403 3403
Size: 6082
AclI AA'CG_TT
Cuts at: 1563 4209 4582 1563
Size: 2646 373 3063
Fragments arranged by size:
3063 2646 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2909 3140 4145 5529 5797 1632
Size: 1277 231 1005 1384 268 1917
Fragments arranged by size:
1917 1384 1277 1005 268 231
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5885
AflIII A'CryG_T
Cuts at: 1203 2081 2681 3184 4981 5958 1203
Size: 878 600 503 1797 977 1327
Fragments arranged by size:
1797 1327 977 878 600 503
AgeI A'CCGG_T
Cuts at: 4913 4913
Size: 6082
AhdI GACnn_n'nnGTC
Cuts at: 4811 4811
Size: 6082
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2142 2805 1184
Size: 958 663 4461
Fragments arranged by size:
4461 958 663
AlwNI CAG_nnn'CTG
Cuts at: 2314 3825 5397 2314
Size: 1511 1572 2999
Fragments arranged by size:
2999 1572 1511
ApaLI G'TGCA_C
Cuts at: 4139 5295 4139
Size: 1156 4926
ApoI r'AATT_y
Cuts at: 1493 2037 2877 3046 3165 3391 3617 3769 5878
Size: 544 840 169 119 226 226 152 2109
Cuts at: 5878 1493
Size: 1697
Fragments arranged by size:
2109 1697 840 544 226 226 169 152
119
AseI AT'TA_AT
Cuts at: 160 1032 4636 160
Size: 872 3604 1606
Fragments arranged by size:
3604 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
AvaI C'yCGr_G
Cuts at: 1870 1943 3407 1870
Size: 73 1464 4545
Fragments arranged by size:
4545 1464 73
AvrII C'CTAG_G
Cuts at: 2221 2221
Size: 6082
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2553 2586 2553
Size: 33 6049
BamHI G'GATC_C
Cuts at: 2061 3412 3779 2061
Size: 1351 367 4364
Fragments arranged by size:
4364 1351 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3403 4858 618
Size: 325 217 627 1616 1455 1842
Fragments arranged by size:
1842 1616 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2862 3351 3401 1391
Size: 1471 489 50 4072
Fragments arranged by size:
4072 1471 489 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5111
BbvCI CC'TCA_GC
Cuts at: 2289 2289
Size: 6082
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2630 2664 4273 4307 1903
Size: 34 50 34 609 34 1609 34 3678
Fragments arranged by size:
3678 1609 609 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3974 5184 3974
Size: 1210 4872
BclI T'GATC_A
Cuts at: 1548 2500 2872 1548
Size: 952 372 4758
Fragments arranged by size:
4758 952 372
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4693 136
Size: 107 122 71 631 605 3021 1525
Fragments arranged by size:
3021 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6077 6077
Size: 6082
BlpI GC'TnA_GC
Cuts at: 3499 3499
Size: 6082
Bme1580I G_kGCm'C
Cuts at: 1790 2439 4143 5299 1790
Size: 649 1704 1156 2573
Fragments arranged by size:
2573 1704 1156 649
BmgBI CAC'GTC
Cuts at: 1318 2440 1318
Size: 1122 4960
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3813 4771 442
Size: 416 53 83 399 24 2396 958 1753
Fragments arranged by size:
2396 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3096 4742 1239
Size: 1857 1646 2579
Fragments arranged by size:
2579 1857 1646
Bpu10I CC'TnA_GC
Cuts at: 2289 2393 2289
Size: 104 5978
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4207 5072 5370 4207
Size: 865 298 4919
Fragments arranged by size:
4919 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2405 3209 4745 882
Size: 617 906 804 1536 2219
Fragments arranged by size:
2219 1536 906 804 617
BsaAI yAC'GTr
Cuts at: 493 1204 2821 3873 493
Size: 711 1617 1052 2702
Fragments arranged by size:
2702 1617 1052 711
BsaBI GATnn'nnATC
Cuts at: 6076 6076
Size: 6082
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3889 4271 4906 275
Size: 53 83 186 936 2356 382 635 1451
Fragments arranged by size:
2356 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2483 4515 4913 5187 5334 5745 2483
Size: 2032 398 274 147 411 2820
Fragments arranged by size:
2820 2032 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5857
Fragments arranged by size:
5857 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2125 3086 3089 1186
Size: 939 961 3 4179
Fragments arranged by size:
4179 961 939 3
BseYI C'CCAG_C
Cuts at: 5285 5781 5285
Size: 496 5586
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2315 2690 1163
Size: 573 579 375 4555
Fragments arranged by size:
4555 579 573 375
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2644 3532 4293 5321 1540
Size: 275 186 643 888 761 1028 2301
Fragments arranged by size:
2301 1028 888 761 643 275 186
BsiHKAI G_wGCw'C
Cuts at: 2014 2862 3351 4143 4228 5299 2014
Size: 848 489 792 85 1071 2797
Fragments arranged by size:
2797 1071 848 792 489 85
BsmI GAATG_Cn'
Cuts at: 2988 3593 3686 2988
Size: 605 93 5384
Fragments arranged by size:
5384 605 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2439 2862 3351 3401 4143 4228
Size: 399 224 425 423 489 50 742 85
Cuts at: 4228 5299 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 489 425 423 399 224
85 50
BspEI T'CCGG_A
Cuts at: 5745 5745
Size: 6082
BspHI T'CATG_A
Cuts at: 3971 5701 3971
Size: 1730 4352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2457 3277 3437 844
Size: 1613 820 160 3489
Fragments arranged by size:
3489 1613 820 160
BsrBI CCG'CTC
Cuts at: 1394 1823 3969 1394
Size: 429 2146 3507
Fragments arranged by size:
3507 2146 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4577 4751 65
Size: 1183 3329 174 1396
Fragments arranged by size:
3329 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3371 4726 4913 1700
Size: 110 1561 1355 187 2869
Fragments arranged by size:
2869 1561 1355 187 110
BsrGI T'GTAC_A
Cuts at: 96 2367 96
Size: 2271 3811
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6082
BssSI C'ACGA_G
Cuts at: 4142 5154 4142
Size: 1012 5070
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2314 794
Size: 1520 4562
BstBI TT'CG_AA
Cuts at: 4886 4886
Size: 6082
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6082
BstYI r'GATC_y
Cuts at: 1972 2061 3412 3779 4164 4181 5633 5719 5731
Size: 89 1351 367 385 17 1452 86 12
Cuts at: 5731 6077 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1351 385 367 346 89 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3001 3001
Size: 6082
BtgI C'CryG_G
Cuts at: 513 1065 2694 513
Size: 552 1629 3901
Fragments arranged by size:
3901 1629 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5343
Fragments arranged by size:
5343 683 56
BtsI GCAGTG_nn'
Cuts at: 2194 3598 4392 4412 2194
Size: 1404 794 20 3864
Fragments arranged by size:
3864 1404 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6082
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2023 2058 529
Size: 35 1459 35 4553
Fragments arranged by size:
4553 1459 35 35
DraI TTT'AAA
Cuts at: 3386 3386
Size: 6082
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5089 809
Size: 507 3773 1802
Fragments arranged by size:
3773 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3529 4418 6047
Size: 54 1425 50 275 18 1699 889 1629
Cuts at: 6047 8
Size: 43
Fragments arranged by size:
1699 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3529 1537
Size: 275 1717 4090
Fragments arranged by size:
4090 1717 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2694 4012 1353
Size: 1341 1318 3423
Fragments arranged by size:
3423 1341 1318
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4669 5043 5189 387
Size: 1064 138 3080 374 146 1280
Fragments arranged by size:
3080 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2860 3349 2860
Size: 489 5593
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2909 3096 3140 4145 4742 5529 5797
Size: 393 1277 187 44 1005 597 787 268
Cuts at: 5797 1239
Size: 1524
Fragments arranged by size:
1524 1277 1005 787 597 393 268 187
44
EcoNI CCTnn'n_nnAGG
Cuts at: 2143 2143
Size: 6082
EcoRI G'AATT_C
Cuts at: 3391 3769 3391
Size: 378 5704
EcoRV GAT'ATC
Cuts at: 3108 3108
Size: 6082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2119 3367 2119
Size: 1248 4834
FspI TGC'GCA
Cuts at: 4588 4588
Size: 6082
HaeII r_GCGC'y
Cuts at: 1678 5229 1678
Size: 3551 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2549 2581 4723
Size: 32 135 32 467 32 478 32 2142
Cuts at: 4723 4755 4797 4829 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2142 478 467 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3426 3672 669
Size: 988 281 1488 246 3079
Fragments arranged by size:
3079 1488 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3152 748
Size: 2404 3678
HpaI GTT'AAC
Cuts at: 3672 3672
Size: 6082
KpnI G_GTAC'C
Cuts at: 3407 3407
Size: 6082
MfeI C'AATT_G
Cuts at: 3681 3681
Size: 6082
MluI A'CGCG_T
Cuts at: 5958 5958
Size: 6082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5196 5380 1305
Size: 3891 184 2007
Fragments arranged by size:
3891 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4260
Fragments arranged by size:
4260 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2733 3207 4040 4399 4558 518
Size: 648 1567 474 833 359 159 2042
Fragments arranged by size:
2042 1567 833 648 474 359 159
MspA1I CmG'CkG
Cuts at: 1606 2311 2326 2418 2713 3064 3498 4175 5323
Size: 705 15 92 295 351 434 677 1148
Cuts at: 5323 5568 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 705 677 434 351 295 245
92 15
NcoI C'CATG_G
Cuts at: 513 1065 2694 513
Size: 552 1629 3901
Fragments arranged by size:
3901 1629 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6082
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6082
NotI GC'GGCC_GC
Cuts at: 3529 3529
Size: 6082
NspI r_CATG'y
Cuts at: 1132 1229 2085 2685 3188 3440 4985 1132
Size: 97 856 600 503 252 1545 2229
Fragments arranged by size:
2229 1545 856 600 503 252 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6082
PciI A'CATG_T
Cuts at: 2081 2681 3184 4981 2081
Size: 600 503 1797 3182
Fragments arranged by size:
3182 1797 600 503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2821 1108
Size: 1713 4369
PmeI GTTT'AAAC
Cuts at: 3386 3386
Size: 6082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4151 4183 5664 5696 4151
Size: 32 1481 32 4537
Fragments arranged by size:
4537 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6082
PsiI TTA'TAA
Cuts at: 3652 3652
Size: 6082
PstI C_TGCA'G
Cuts at: 830 2208 2319 3288 3434 830
Size: 1378 111 969 146 3478
Fragments arranged by size:
3478 1378 969 146 111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6082
PvuII CAG'CTG
Cuts at: 2713 3064 2713
Size: 351 5731
SacI G_AGCT'C
Cuts at: 2862 3351 2862
Size: 489 5593
SalI G'TCGA_C
Cuts at: 1936 3424 1936
Size: 1488 4594
SapI GCTCTTCn'nnn_
Cuts at: 2694 2694
Size: 6082
SbfI CC_TGCA'GG
Cuts at: 3434 3434
Size: 6082
ScaI AGT'ACT
Cuts at: 4330 6042 4330
Size: 1712 4370
SfcI C'TryA_G
Cuts at: 826 1045 2204 2315 2587 3284 3430 4565 5246
Size: 219 1159 111 272 697 146 1135 681
Cuts at: 5246 5437 826
Size: 191 1471
Fragments arranged by size:
1471 1159 1135 697 681 272 219 191
146 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6082
SmaI CCC'GGG
Cuts at: 1872 3409 1872
Size: 1537 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4186 5087 5349 820
Size: 197 926 2243 901 262 1553
Fragments arranged by size:
2243 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3873 493
Size: 3380 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6082
SphI G_CATG'C
Cuts at: 1132 3440 1132
Size: 2308 3774
SspI AAT'ATT
Cuts at: 5 52 4006 5
Size: 47 3954 2081
Fragments arranged by size:
3954 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6082
StyI C'CwwG_G
Cuts at: 513 1065 2144 2221 2694 3137 3219 3521 513
Size: 552 1079 77 473 443 82 302 3074
Fragments arranged by size:
3074 1079 552 473 443 302 82 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2658 3083 3132 4109 4126 4279 4464
Size: 1255 1078 425 49 977 17 153 185
Cuts at: 4464 4909 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1078 977 445 425 185 153
49 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2367 4328 6040
Size: 274 80 33 51 1443 390 1961 1712
Cuts at: 6040 96
Size: 138
Fragments arranged by size:
1961 1712 1443 390 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2762 2794 6042 6074 2762
Size: 32 3248 32 2770
Fragments arranged by size:
3248 2770 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6082
XbaI T'CTAG_A
Cuts at: 3418 3418
Size: 6082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2818 3120 2818
Size: 302 5780
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6082
XmaI C'CCGG_G
Cuts at: 1870 3407 1870
Size: 1537 4545
XmnI GAAnn'nnTTC
Cuts at: 4211 4211
Size: 6082
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3890 276
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI