Restriction map for
pFN21AE0936
(Circular) (Six-base) MAPSORT of: pFN21AE0936.seq Check: 8170 from: 1 to: 5959
pFN21AE0936 5959 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2055 2271 3302 1937
Size: 118 216 1031 4594
Fragments arranged by size:
4594 1031 216 118
Acc65I G'GTAC_C
Cuts at: 2080 3280 2080
Size: 1200 4759
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2134 2237 3062 4022 5406 5674 1632
Size: 502 103 825 960 1384 268 1917
Fragments arranged by size:
1917 1384 960 825 502 268 103
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 2972 3238 3702 5274 2972
Size: 266 464 1572 3657
Fragments arranged by size:
3657 1572 464 266
ApaI G_GGCC'C
Cuts at: 2496 2496
Size: 5959
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2347 3268 3494 3646 5755 1493
Size: 854 921 226 152 2109 1697
Fragments arranged by size:
2109 1697 921 854 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2037 2937 3284 1870
Size: 73 94 900 347 4545
Fragments arranged by size:
4545 900 347 94 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 3280 4735 618
Size: 325 217 627 293 1200 1455 1842
Fragments arranged by size:
1842 1455 1200 627 325 293 217
BanII G_rGCy'C
Cuts at: 1391 2496 2641 2666 3059 3074 3195 3278 1391
Size: 1105 145 25 393 15 121 83 4072
Fragments arranged by size:
4072 1105 393 145 121 83 25 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2431 2733 928
Size: 971 532 302 4154
Fragments arranged by size:
4154 971 532 302
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 2190 1548
Size: 642 5317
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2780 4570 136
Size: 107 122 71 631 605 1108 1790 1525
Fragments arranged by size:
1790 1525 1108 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 2496 4020 5176 1790
Size: 706 1524 1156 2573
Fragments arranged by size:
2573 1524 1156 706
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2570 2604 2745
Size: 416 53 83 399 24 1153 34 141
Cuts at: 2745 2854 3690 4648 442
Size: 109 836 958 1753
Fragments arranged by size:
1753 1153 958 836 416 399 141 109
83 53 34 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2825
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2353 2728 2757 4619 1239
Size: 1114 375 29 1862 2579
Fragments arranged by size:
2579 1862 1114 375 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4949 5247 4084
Size: 865 298 4796
Fragments arranged by size:
4796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 2120 2414 5953 2120
Size: 294 3539 2126
Fragments arranged by size:
3539 2126 294
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3222 3766 4148 4783
Size: 53 83 186 936 1689 544 382 635
Cuts at: 4783 275
Size: 1451
Fragments arranged by size:
1689 1451 936 635 544 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3153 4392 4790 5064 5211 5622 3153
Size: 1239 398 274 147 411 3490
Fragments arranged by size:
3490 1239 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2593 2623 2648 2678 1178
Size: 30 165 30 1190 30 25 30 4459
Fragments arranged by size:
4459 1190 165 30 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2649 2817 2857 2953 3191 3224 1186
Size: 1463 168 40 96 238 33 3921
Fragments arranged by size:
3921 1463 238 168 96 40 33
BseYI C'CCAG_C
Cuts at: 2693 3110 5162 5658 2693
Size: 417 2052 496 2994
Fragments arranged by size:
2994 2052 496 417
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2405 1163
Size: 573 669 4717
Fragments arranged by size:
4717 669 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2666 3195 4020 4105 5176 2666
Size: 529 825 85 1071 3449
Fragments arranged by size:
3449 1071 825 529 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
BsmBI CGTCTCn'nnnn_
Cuts at: 2545 2545
Size: 5959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2496 2641 2666 3059 3074 3195 3278
Size: 399 706 145 25 393 15 121 83
Cuts at: 3278 4020 4105 5176 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 706 399 393 145 121
85 83 25 15
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 2343 3848 5578 2343
Size: 1505 1730 2724
Fragments arranged by size:
2724 1730 1505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2173 3314 844
Size: 1329 1141 3489
Fragments arranged by size:
3489 1329 1141
BsrBI CCG'CTC
Cuts at: 1394 1823 2992 3846 1394
Size: 429 1169 854 3507
Fragments arranged by size:
3507 1169 854 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2328 4454 4628 65
Size: 1183 1080 2126 174 1396
Fragments arranged by size:
2126 1396 1183 1080 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2908 3168 3289 3656 4041 4058 5510 5596
Size: 936 260 121 367 385 17 1452 86
Cuts at: 5596 5608 5954 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 936 385 367 346 260 121
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3255 513
Size: 552 939 1251 3217
Fragments arranged by size:
3217 1251 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 2133 2621 3475 4269 4289 2133
Size: 488 854 794 20 3803
Fragments arranged by size:
3803 854 794 488 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DraIII CAC_nnn'GTG
Cuts at: 3202 3202
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2764 4966 809
Size: 507 1448 2202 1802
Fragments arranged by size:
2202 1802 1448 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2095 2484
Size: 54 1425 50 275 18 177 88 389
Cuts at: 2484 2679 2720 2835 2974 3406 4295 5924 8
Size: 195 41 115 139 432 889 1629 43
Fragments arranged by size:
1629 1425 889 432 389 275 195 177
139 115 88 54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2292 2704 3889 1353
Size: 939 412 1185 3423
Fragments arranged by size:
3423 1185 939 412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2814 4546 4920 5066 387
Size: 1064 138 1225 1732 374 146 1280
Fragments arranged by size:
1732 1280 1225 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2664 3193 2664
Size: 529 5430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2134 2237 2353 2728 2757 3062 4022
Size: 393 502 103 116 375 29 305 960
Cuts at: 4022 4619 5406 5674 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 960 787 597 502 393 375 305
268 116 103 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2813 3176 2813
Size: 363 5596
EcoO109I rG'GnC_Cy
Cuts at: 2363 2492 2757 2934 2363
Size: 129 265 177 5388
Fragments arranged by size:
5388 265 177 129
EcoRI G'AATT_C
Cuts at: 2347 3268 3646 2347
Size: 921 378 4660
Fragments arranged by size:
4660 921 378
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 2535 5106 1678
Size: 857 2571 2531
Fragments arranged by size:
2571 2531 857
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2648 2680 4600 4632 4674
Size: 32 135 32 1076 32 1920 32 42
Cuts at: 4674 4706 1373
Size: 32 2626
Fragments arranged by size:
2626 1920 1076 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 2084 3284 2084
Size: 1200 4759
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2140 2740 5073 5257 1305
Size: 835 600 2333 184 2007
Fragments arranged by size:
2333 2007 835 600 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2097 2837 10
Size: 54 1425 343 177 88 740 3132
Fragments arranged by size:
3132 1425 740 343 177 88 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3917 4276 4435 518
Size: 648 2751 359 159 2042
Fragments arranged by size:
2751 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2684 2790 3375 4052 5200 5445 1606
Size: 1078 106 585 677 1148 245 2120
Fragments arranged by size:
2120 1148 1078 677 585 245 106
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4468
Fragments arranged by size:
4468 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3317 4862 1132
Size: 97 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2641 2985 1348
Size: 1293 344 4322
Fragments arranged by size:
4322 1293 344
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2594 1108
Size: 1486 4473
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2757 2934 2757
Size: 177 5782
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PspOMI G'GGCC_C
Cuts at: 2492 2492
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2527 3311 830
Size: 1697 784 3478
Fragments arranged by size:
3478 1697 784
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SacI G_AGCT'C
Cuts at: 2666 3195 2666
Size: 529 5430
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SanDI GG'GwC_CC
Cuts at: 2934 2934
Size: 5959
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2523 3307 4442 5123 5314 826
Size: 219 1478 784 1135 681 191 1471
Fragments arranged by size:
1478 1471 1135 784 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 2039 3286 1872
Size: 167 1247 4545
Fragments arranged by size:
4545 1247 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4063 4964 5226 820
Size: 197 926 2120 901 262 1553
Fragments arranged by size:
2120 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SrfI GCCC'GGGC
Cuts at: 2039 2039
Size: 5959
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 2808 1517
Size: 1291 4668
StyI C'CwwG_G
Cuts at: 513 1065 2004 3398 513
Size: 552 939 1394 3074
Fragments arranged by size:
3074 1394 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2605 3986 4003 4156 4341 4786 325
Size: 1255 1025 1381 17 153 185 445 1498
Fragments arranged by size:
1498 1381 1255 1025 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2220 4205 5917
Size: 274 80 33 51 1443 243 1985 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
1985 1712 1443 274 243 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2562 2594 5919 5951 2562
Size: 32 3325 32 2570
Fragments arranged by size:
3325 2570 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 2467 3295 2467
Size: 828 5131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2674 2674
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 2037 3284 1870
Size: 167 1247 4545
Fragments arranged by size:
4545 1247 167
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII
SapI SexAI SfiI SfoI SwaI