Restriction map for
pFN21AE0933
(Circular) (Six-base) MAPSORT of: pFN21AE0933.seq Check: 9033 from: 1 to: 5950
pFN21AE0933 5950 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2691 2857 3293 1937
Size: 754 166 436 4594
Fragments arranged by size:
4594 754 436 166
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4013 5397 5665 1632
Size: 2381 1384 268 1917
Fragments arranged by size:
2381 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 4849 5826 1203
Size: 3646 977 1327
Fragments arranged by size:
3646 1327 977
AgeI A'CCGG_T
Cuts at: 4781 4781
Size: 5950
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 2175 2652 1166
Size: 1009 477 4464
Fragments arranged by size:
4464 1009 477
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5950
AlwNI CAG_nnn'CTG
Cuts at: 2065 3693 5265 2065
Size: 1628 1572 2750
Fragments arranged by size:
2750 1628 1572
ApaLI G'TGCA_C
Cuts at: 2373 4007 5163 2373
Size: 1634 1156 3160
Fragments arranged by size:
3160 1634 1156
ApoI r'AATT_y
Cuts at: 1493 2076 3259 3485 3637 5746 1493
Size: 583 1183 226 152 2109 1697
Fragments arranged by size:
2109 1697 1183 583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2211 4504 160
Size: 872 1179 2293 1606
Fragments arranged by size:
2293 1606 1179 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 3275 1870
Size: 73 1332 4545
Fragments arranged by size:
4545 1332 73
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2675 3271 4726 618
Size: 325 217 627 888 596 1455 1842
Fragments arranged by size:
1842 1455 888 627 596 325 217
BanII G_rGCy'C
Cuts at: 1391 3269 1391
Size: 1878 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2324 2895 928
Size: 971 425 571 3983
Fragments arranged by size:
3983 971 571 425
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4141 4175 1903
Size: 34 50 34 2120 34 3678
Fragments arranged by size:
3678 2120 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2035 3842 5052 2035
Size: 1807 1210 2933
Fragments arranged by size:
2933 1807 1210
BclI T'GATC_A
Cuts at: 1548 2167 2908 1548
Size: 619 741 4590
Fragments arranged by size:
4590 741 619
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4561 136
Size: 107 122 71 631 605 2889 1525
Fragments arranged by size:
2889 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 3367 3367
Size: 5950
Bme1580I G_kGCm'C
Cuts at: 1790 2377 4011 5167 1790
Size: 587 1634 1156 2573
Fragments arranged by size:
2573 1634 1156 587
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5950
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3681 4639 442
Size: 416 53 83 399 24 2264 958 1753
Fragments arranged by size:
2264 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4610 1239
Size: 3371 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4613 882
Size: 617 3114 2219
Fragments arranged by size:
3114 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3741 493
Size: 711 2537 2702
Fragments arranged by size:
2702 2537 711
BsaBI GATnn'nnATC
Cuts at: 5944 5944
Size: 5950
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3757 4139 4774 275
Size: 53 83 186 936 2224 382 635 1451
Fragments arranged by size:
2224 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4383 4781 5055 5202 5613 4383
Size: 398 274 147 411 4720
Fragments arranged by size:
4720 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5725
Fragments arranged by size:
5725 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3044 1186
Size: 1858 4092
BseYI C'CCAG_C
Cuts at: 5153 5649 5153
Size: 496 5454
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2676 3119 1163
Size: 573 940 443 3994
Fragments arranged by size:
3994 940 573 443
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3400 4161 5189 1540
Size: 275 186 1399 761 1028 2301
Fragments arranged by size:
2301 1399 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2377 3049 4011 4096 5167 2377
Size: 672 962 85 1071 3160
Fragments arranged by size:
3160 1071 962 672 85
BsmI GAATG_Cn'
Cuts at: 3461 3554 3461
Size: 93 5857
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2377 3049 3269 4011 4096 5167 1391
Size: 399 587 672 220 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 672 587 399 220 85
BspEI T'CCGG_A
Cuts at: 5613 5613
Size: 5950
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3305 844
Size: 2461 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2023 3837 1394
Size: 429 200 1814 3507
Fragments arranged by size:
3507 1814 429 200
BsrDI GCAATG_nn'
Cuts at: 65 1248 4445 4619 65
Size: 1183 3197 174 1396
Fragments arranged by size:
3197 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4594 4781 1700
Size: 110 2784 187 2869
Fragments arranged by size:
2869 2784 187 110
BsrGI T'GTAC_A
Cuts at: 96 2993 96
Size: 2897 3053
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 4010 5022 4010
Size: 1012 4938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5950
BstBI TT'CG_AA
Cuts at: 2031 2080 4754 2031
Size: 49 2674 3227
Fragments arranged by size:
3227 2674 49
BstEII G'GTnAC_C
Cuts at: 1184 2984 1184
Size: 1800 4150
BstYI r'GATC_y
Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945
Size: 1308 367 385 17 1452 86 12 346
Cuts at: 5945 1972
Size: 1977
Fragments arranged by size:
1977 1452 1308 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2858 2858
Size: 5950
BtgI C'CryG_G
Cuts at: 513 1065 2629 513
Size: 552 1564 3834
Fragments arranged by size:
3834 1564 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5211
Fragments arranged by size:
5211 683 56
BtsI GCAGTG_nn'
Cuts at: 3466 4260 4280 3466
Size: 794 20 5136
Fragments arranged by size:
5136 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5915
DraI TTT'AAA
Cuts at: 2413 2596 3254 2413
Size: 183 658 5109
Fragments arranged by size:
5109 658 183
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 2384 2543
Size: 54 1425 50 275 18 282 272 159
Cuts at: 2543 3397 4286 5915 8
Size: 854 889 1629 43
Fragments arranged by size:
1629 1425 889 854 282 275 272 159
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 2229 3880 1353
Size: 675 201 1651 3423
Fragments arranged by size:
3423 1651 675 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4537 4911 5057 387
Size: 1064 138 2948 374 146 1280
Fragments arranged by size:
2948 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4013 4610 5397 5665 1239
Size: 393 2381 597 787 268 1524
Fragments arranged by size:
2381 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2908 2908
Size: 5950
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 2671 3210 5097 1678
Size: 993 539 1887 2531
Fragments arranged by size:
2531 1887 993 539
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2441 2473 2776
Size: 32 135 32 773 32 64 32 303
Cuts at: 2776 2808 4591 4623 4665 4697 1373
Size: 32 1783 32 42 32 2626
Fragments arranged by size:
2626 1783 773 303 135 64 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2227 3294 3540 669
Size: 988 281 289 1067 246 3079
Fragments arranged by size:
3079 1067 988 289 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5950
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2019 5064 5248 1305
Size: 714 3045 184 2007
Fragments arranged by size:
3045 2007 714 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2114 2386 2545 10
Size: 54 1425 343 282 272 159 3415
Fragments arranged by size:
3415 1425 343 282 272 159 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 2652 3176 3908 4267 4426 518
Size: 648 1009 477 524 732 359 159 2042
Fragments arranged by size:
2042 1009 732 648 524 477 359 159
MspA1I CmG'CkG
Cuts at: 1606 2065 3366 4043 5191 5436 1606
Size: 459 1301 677 1148 245 2120
Fragments arranged by size:
2120 1301 1148 677 459 245
NcoI C'CATG_G
Cuts at: 513 1065 2629 513
Size: 552 1564 3834
Fragments arranged by size:
3834 1564 552
NdeI CA'TA_TG
Cuts at: 387 2164 387
Size: 1777 4173
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 2286 3308 4853 1132
Size: 97 1057 1022 1545 2229
Fragments arranged by size:
2229 1545 1057 1022 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 4849 4849
Size: 5950
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2552 1108
Size: 1444 4506
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2012 2044 2506 2538 4019 4051 5532 5564 2012
Size: 32 462 32 1481 32 1481 32 2398
Fragments arranged by size:
2398 1481 1481 462 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2697 1613
Size: 1084 4866
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PstI C_TGCA'G
Cuts at: 830 3302 830
Size: 2472 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 2381 4198 5910 2381
Size: 1817 1712 2421
Fragments arranged by size:
2421 1817 1712
SfcI C'TryA_G
Cuts at: 826 1045 2650 3298 4433 5114 5305 826
Size: 219 1605 648 1135 681 191 1471
Fragments arranged by size:
1605 1471 1135 681 648 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 3277 1872
Size: 1405 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4054 4955 5217 820
Size: 197 926 2111 901 262 1553
Fragments arranged by size:
2111 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3308 1132
Size: 2176 3774
SspI AAT'ATT
Cuts at: 5 52 3874 5
Size: 47 3822 2081
Fragments arranged by size:
3822 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5950
StyI C'CwwG_G
Cuts at: 513 1065 2629 3389 513
Size: 552 1564 760 3074
Fragments arranged by size:
3074 1564 760 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3003 3977 3994 4147 4332 4777 325
Size: 1255 1423 974 17 153 185 445 1498
Fragments arranged by size:
1498 1423 1255 974 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2067 2379 2470
Size: 274 80 33 51 1443 90 312 91
Cuts at: 2470 2993 4196 5908 96
Size: 523 1203 1712 138
Fragments arranged by size:
1712 1443 1203 523 312 274 138 91
90 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2415 2447 5910 5942 2415
Size: 32 3463 32 2423
Fragments arranged by size:
3463 2423 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5950
XbaI T'CTAG_A
Cuts at: 2962 3286 2962
Size: 324 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 3275 1870
Size: 1405 4545
XmnI GAAnn'nnTTC
Cuts at: 4079 4079
Size: 5950
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI