Restriction map for
pFN21AE0926
(Circular) (Six-base) MAPSORT of: pFN21AE0926.seq Check: 765 from: 1 to: 5782
pFN21AE0926 5782 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2570 3125 1937
Size: 633 555 4594
Fragments arranged by size:
4594 633 555
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2382 2549 3845 5229 5497 1632
Size: 750 167 1296 1384 268 1917
Fragments arranged by size:
1917 1384 1296 750 268 167
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 2476 4681 5658 1203
Size: 1273 2205 977 1327
Fragments arranged by size:
2205 1327 1273 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 3049 4511 3049
Size: 1462 4320
AleI CACnn'nnGTG
Cuts at: 1166 2829 1166
Size: 1663 4119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2106 1184
Size: 922 4860
AlwNI CAG_nnn'CTG
Cuts at: 2432 2557 2743 3525 5097 2432
Size: 125 186 782 1572 3117
Fragments arranged by size:
3117 1572 782 186 125
ApaI G_GGCC'C
Cuts at: 2597 2597
Size: 5782
ApaLI G'TGCA_C
Cuts at: 3010 3839 4995 3010
Size: 829 1156 3797
Fragments arranged by size:
3797 1156 829
ApoI r'AATT_y
Cuts at: 1493 2685 2945 3091 3317 3469 5578 1493
Size: 1192 260 146 226 152 2109 1697
Fragments arranged by size:
2109 1697 1192 260 226 152 146
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 3107 1870
Size: 73 1164 4545
Fragments arranged by size:
4545 1164 73
BamHI G'GATC_C
Cuts at: 2723 3112 3479 2723
Size: 389 367 5026
Fragments arranged by size:
5026 389 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3103 4558 618
Size: 325 217 627 1316 1455 1842
Fragments arranged by size:
1842 1455 1316 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2214 2597 2967 3101 1391
Size: 823 383 370 134 4072
Fragments arranged by size:
4072 823 383 370 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2340 2537 2821 928
Size: 971 441 197 284 3889
Fragments arranged by size:
3889 971 441 284 197
BbvCI CC'TCA_GC
Cuts at: 2728 2728
Size: 5782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3047 3081 3973 4007 1903
Size: 34 1110 34 892 34 3678
Fragments arranged by size:
3678 1110 892 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4393 136
Size: 107 122 71 631 605 2721 1525
Fragments arranged by size:
2721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2469 5777 2469
Size: 3308 2474
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 2597 3014 3843 4999 1790
Size: 807 417 829 1156 2573
Fragments arranged by size:
2573 1156 829 807 417
BmgBI CAC'GTC
Cuts at: 1318 2376 1318
Size: 1058 4724
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2534 3513 4471
Size: 416 53 83 399 24 1117 979 958
Cuts at: 4471 442
Size: 1753
Fragments arranged by size:
1753 1117 979 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2310 1056
Size: 1254 4528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 4442 1239
Size: 1043 2160 2579
Fragments arranged by size:
2579 2160 1043
Bpu10I CC'TnA_GC
Cuts at: 2728 2728
Size: 5782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3589 3971 4606 275
Size: 53 83 186 936 2056 382 635 1451
Fragments arranged by size:
2056 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2651 2681 1178
Size: 30 165 30 1248 30 4279
Fragments arranged by size:
4279 1248 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2283 2434 2640 2717 1186
Size: 1097 151 206 77 4251
Fragments arranged by size:
4251 1097 206 151 77
BseYI C'CCAG_C
Cuts at: 2182 4985 5481 2182
Size: 2803 496 2483
Fragments arranged by size:
2803 2483 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2570 1163
Size: 573 834 4375
Fragments arranged by size:
4375 834 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2396 2456 2967 3014 3843 3928 4999 2396
Size: 60 511 47 829 85 1071 3179
Fragments arranged by size:
3179 1071 829 511 85 60 47
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2396 2456 2597 2967 3014 3101
Size: 399 424 182 60 141 370 47 87
Cuts at: 3101 3843 3928 4999 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 424 399 370 182 141
87 85 60 47
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2062 2074 3137 844
Size: 1218 12 1063 3489
Fragments arranged by size:
3489 1218 1063 12
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2778 4277 4451 65
Size: 1183 1530 1499 174 1396
Fragments arranged by size:
1530 1499 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 2856 96
Size: 2760 3022
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2557 2743 3018 794
Size: 1763 186 275 3558
Fragments arranged by size:
3558 1763 275 186
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2542 2173
Size: 369 5413
BstYI r'GATC_y
Cuts at: 1972 2110 2469 2723 3112 3479 3864 3881 5333
Size: 138 359 254 389 367 385 17 1452
Cuts at: 5333 5419 5431 5777 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 389 385 367 359 346 254
138 86 17 12
BstZ17I GTA'TAC
Cuts at: 2571 2571
Size: 5782
BtgI C'CryG_G
Cuts at: 513 1065 2004 2172 2562 3078 513
Size: 552 939 168 390 516 3217
Fragments arranged by size:
3217 939 552 516 390 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3044 487
Size: 683 56 1818 3225
Fragments arranged by size:
3225 1818 683 56
BtsI GCAGTG_nn'
Cuts at: 2088 3298 4092 4112 2088
Size: 1210 794 20 3758
Fragments arranged by size:
3758 1210 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3229 4118 5747
Size: 54 1425 50 275 18 1399 889 1629
Cuts at: 5747 8
Size: 43
Fragments arranged by size:
1629 1425 1399 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2787 2804 3712 1353
Size: 1434 17 908 3423
Fragments arranged by size:
3423 1434 908 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2965 2965
Size: 5782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 2382 2549 3845 4442 5229 5497
Size: 393 650 100 167 1296 597 787 268
Cuts at: 5497 1239
Size: 1524
Fragments arranged by size:
1524 1296 787 650 597 393 268 167
100
EcoO109I rG'GnC_Cy
Cuts at: 2593 2594 2593
Size: 1 5781
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2816 2816
Size: 5782
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 4929 1678
Size: 3251 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2431 2463 2651 2683 2708
Size: 32 135 32 859 32 188 32 25
Cuts at: 2708 2740 2921 2953 3035 3067 4423 4455 4497
Size: 32 181 32 82 32 1356 32 42
Cuts at: 4497 4529 1373
Size: 32 2626
Fragments arranged by size:
2626 1356 859 188 181 135 82 42
32 32 32 32 32 32 32 32
32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 2266 2379 2659 3126 3372 669
Size: 988 281 328 113 280 467 246 3079
Fragments arranged by size:
3079 988 467 328 281 280 246 113
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 2712 3381 2712
Size: 669 5113
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2068 2174 4896 5080 1305
Size: 763 106 2722 184 2007
Fragments arranged by size:
2722 2007 763 184 106
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2540 2669 2829 3740 4099 4258 518
Size: 648 1374 129 160 911 359 159 2042
Fragments arranged by size:
2042 1374 911 648 359 160 159 129
MspA1I CmG'CkG
Cuts at: 1606 3198 3875 5023 5268 1606
Size: 1592 677 1148 245 2120
Fragments arranged by size:
2120 1592 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2172 2562 3078 513
Size: 552 939 168 390 516 3217
Fragments arranged by size:
3217 939 552 516 390 168
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 2306 1052
Size: 1254 4528
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 2314 2480 3140 4685 1132
Size: 97 1085 166 660 1545 2229
Fragments arranged by size:
2229 1545 1085 660 166 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 2476 4681 2476
Size: 2205 3577
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2621 1108
Size: 1513 4269
PfoI T'CCnGG_A
Cuts at: 2157 2939 2157
Size: 782 5000
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 2916 3352 2916
Size: 436 5346
PspOMI G'GGCC_C
Cuts at: 2593 2593
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2587 2602 2748 3134 830
Size: 1757 15 146 386 3478
Fragments arranged by size:
3478 1757 386 146 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
SacI G_AGCT'C
Cuts at: 2967 2967
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2583 2598 2744 2753 3130 4265 4946
Size: 219 1538 15 146 9 377 1135 681
Cuts at: 4946 5137 826
Size: 191 1471
Fragments arranged by size:
1538 1471 1135 681 377 219 191 146
15 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3886 4787 5049 820
Size: 197 926 1943 901 262 1553
Fragments arranged by size:
1943 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 2314 3140 1132
Size: 1182 826 3774
Fragments arranged by size:
3774 1182 826
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2004 2172 2286 2562 3078 3221 513
Size: 552 939 168 114 276 516 143 3074
Fragments arranged by size:
3074 939 552 516 276 168 143 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2510 3809 3826 3979 4164 4609 325
Size: 1255 930 1299 17 153 185 445 1498
Fragments arranged by size:
1498 1299 1255 930 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2708 2856 4028
Size: 274 80 33 51 1443 731 148 1172
Cuts at: 4028 5740 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1172 731 274 148 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5742 5774 5742
Size: 32 5750
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2677 2011
Size: 666 5116
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI