Restriction map for
pFN21AE0925
(Circular) (Six-base) MAPSORT of: pFN21AE0925.seq Check: 4399 from: 1 to: 5689
pFN21AE0925 5689 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3752 5136 5404 1632
Size: 2120 1384 268 1917
Fragments arranged by size:
2120 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 2204 3432 5004 2204
Size: 1228 1572 2889
Fragments arranged by size:
2889 1572 1228
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2128 2351 2453 2649 2998 3224 3376 5485
Size: 635 223 102 196 349 226 152 2109
Cuts at: 5485 1493
Size: 1697
Fragments arranged by size:
2109 1697 635 349 226 223 196 152
102
AseI AT'TA_AT
Cuts at: 160 1032 2256 4243 160
Size: 872 1224 1987 1606
Fragments arranged by size:
1987 1606 1224 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 3014 1870
Size: 73 105 966 4545
Fragments arranged by size:
4545 966 105 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2618 2651 2618
Size: 33 5656
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2028 3010 4465 618
Size: 325 217 627 241 982 1455 1842
Fragments arranged by size:
1842 1455 982 627 325 241 217
BanII G_rGCy'C
Cuts at: 1391 2067 2095 3008 1391
Size: 676 28 913 4072
Fragments arranged by size:
4072 913 676 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2047 2471 928
Size: 971 148 424 4146
Fragments arranged by size:
4146 971 424 148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2619 2740 3581 4791 2619
Size: 121 841 1210 3517
Fragments arranged by size:
3517 1210 841 121
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 2244 3106 2244
Size: 862 4827
Bme1580I G_kGCm'C
Cuts at: 1790 3750 4906 1790
Size: 1960 1156 2573
Fragments arranged by size:
2573 1960 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4349 1239
Size: 3110 2579
Bpu10I CC'TnA_GC
Cuts at: 2976 2976
Size: 5689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2259 3814 4679 4977 2259
Size: 1555 865 298 2971
Fragments arranged by size:
2971 1555 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 2751 5683 2751
Size: 2932 2757
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 3878 4513 275
Size: 53 83 186 936 1963 382 635 1451
Fragments arranged by size:
1963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2082 2112 1178
Size: 30 165 30 679 30 4755
Fragments arranged by size:
4755 679 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2103 1186
Size: 917 4772
BseYI C'CCAG_C
Cuts at: 4892 5388 4892
Size: 496 5193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2619 1163
Size: 573 883 4233
Fragments arranged by size:
4233 883 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2067 3750 3835 4906 2067
Size: 1683 85 1071 2850
Fragments arranged by size:
2850 1683 1071 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2095 3008 3750 3835 4906 1391
Size: 399 277 28 913 742 85 1071 2174
Fragments arranged by size:
2174 1071 913 742 399 277 85 28
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2239 3044 844
Size: 1395 805 3489
Fragments arranged by size:
3489 1395 805
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2020 4184 4358 65
Size: 1183 772 2164 174 1396
Fragments arranged by size:
2164 1396 1183 772 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4333 4520 1700
Size: 110 2523 187 2869
Fragments arranged by size:
2869 2523 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684
Size: 1047 367 385 17 1452 86 12 346
Cuts at: 5684 1972
Size: 1977
Fragments arranged by size:
1977 1452 1047 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2742 513
Size: 552 1677 3460
Fragments arranged by size:
3460 1677 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 3205 3999 4019 3205
Size: 794 20 4875
Fragments arranged by size:
4875 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2347 2993 2347
Size: 646 5043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2051 4696 809
Size: 507 735 2645 1802
Fragments arranged by size:
2645 1802 735 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2667 3136 4025
Size: 54 1425 50 275 18 837 469 889
Cuts at: 4025 5654 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 837 469 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3619 1353
Size: 2266 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2065 2065
Size: 5689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3752 4349 5136 5404 1239
Size: 393 2120 597 787 268 1524
Fragments arranged by size:
2120 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 4836 1678
Size: 3158 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 4330 4362 4404
Size: 32 135 32 749 32 1977 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 1977 749 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4803 4987 1305
Size: 3498 184 2007
Fragments arranged by size:
3498 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 10
Size: 54 1425 343 837 3030
Fragments arranged by size:
3030 1425 837 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 3647 4006 4165 518
Size: 648 1147 1334 359 159 2042
Fragments arranged by size:
2042 1334 1147 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3105 3782 4930 5175 1606
Size: 1499 677 1148 245 2120
Fragments arranged by size:
2120 1499 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2742 513
Size: 552 1677 3460
Fragments arranged by size:
3460 1677 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 2577 3047 4592 1132
Size: 97 1348 470 1545 2229
Fragments arranged by size:
2229 1545 1348 470 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2153 1108
Size: 1045 4644
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PstI C_TGCA'G
Cuts at: 830 3041 830
Size: 2211 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
SacI G_AGCT'C
Cuts at: 2067 2067
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 3037 4172 4853 5044 826
Size: 219 1992 1135 681 191 1471
Fragments arranged by size:
1992 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 2050 3016 1872
Size: 178 966 4545
Fragments arranged by size:
4545 966 178
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2274 3793 4694 4956 820
Size: 197 926 331 1519 901 262 1553
Fragments arranged by size:
1553 1519 926 901 331 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 2935 152
Size: 2783 2906
SphI G_CATG'C
Cuts at: 1132 2577 3047 1132
Size: 1445 470 3774
Fragments arranged by size:
3774 1445 470
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 2608 1517
Size: 1091 4598
StyI C'CwwG_G
Cuts at: 513 1065 2742 3128 513
Size: 552 1677 386 3074
Fragments arranged by size:
3074 1677 552 386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2572 3716 3733 3886 4071 4516 325
Size: 1255 992 1144 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1144 992 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2419 2634 2824
Size: 274 80 33 51 1443 442 215 190
Cuts at: 2824 3935 5647 96
Size: 1111 1712 138
Fragments arranged by size:
1712 1443 1111 442 274 215 190 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5649 5681 5649
Size: 32 5657
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 2048 3014 1870
Size: 178 966 4545
Fragments arranged by size:
4545 966 178
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI