Restriction map for
pFN21AE0924
(Circular) (Six-base) MAPSORT of: pFN21AE0924.seq Check: 765 from: 1 to: 5734
pFN21AE0924 5734 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3077 1937
Size: 1140 4594
Acc65I G'GTAC_C
Cuts at: 2471 3055 2471
Size: 584 5150
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 2328 3797 5181 5449 1632
Size: 641 55 1469 1384 268 1917
Fragments arranged by size:
1917 1469 1384 641 268 55
AflII C'TTAA_G
Cuts at: 820 1017 2120 820
Size: 197 1103 4434
Fragments arranged by size:
4434 1103 197
AflIII A'CryG_T
Cuts at: 1203 4633 5610 1203
Size: 3430 977 1327
Fragments arranged by size:
3430 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 4463 4463
Size: 5734
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5734
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2528 2560 2528
Size: 32 5702
AlwNI CAG_nnn'CTG
Cuts at: 2710 2995 3477 5049 2710
Size: 285 482 1572 3395
Fragments arranged by size:
3395 1572 482 285
ApaLI G'TGCA_C
Cuts at: 2395 3791 4947 2395
Size: 1396 1156 3182
Fragments arranged by size:
3182 1396 1156
ApoI r'AATT_y
Cuts at: 1493 2914 3043 3269 3421 5530 1493
Size: 1421 129 226 152 2109 1697
Fragments arranged by size:
2109 1697 1421 226 152 129
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 2466 3059 1870
Size: 73 523 593 4545
Fragments arranged by size:
4545 593 523 73
AvrII C'CTAG_G
Cuts at: 2152 2152
Size: 5734
BamHI G'GATC_C
Cuts at: 2115 3064 3431 2115
Size: 949 367 4418
Fragments arranged by size:
4418 949 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2471 3055 4510 618
Size: 325 217 627 684 584 1455 1842
Fragments arranged by size:
1842 1455 684 627 584 325 217
BanII G_rGCy'C
Cuts at: 1391 2025 3053 1391
Size: 634 1028 4072
Fragments arranged by size:
4072 1028 634
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4763
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3925 3959 1903
Size: 34 1988 34 3678
Fragments arranged by size:
3678 1988 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2047 2571 3626 4836 2047
Size: 524 1055 1210 2945
Fragments arranged by size:
2945 1210 1055 524
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4345 136
Size: 107 122 71 631 605 2673 1525
Fragments arranged by size:
2673 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5729 5729
Size: 5734
BlpI GC'TnA_GC
Cuts at: 3151 3151
Size: 5734
Bme1580I G_kGCm'C
Cuts at: 1790 2399 2772 3795 4951 1790
Size: 609 373 1023 1156 2573
Fragments arranged by size:
2573 1156 1023 609 373
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5734
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2331 2810 3465
Size: 416 53 83 399 24 914 479 655
Cuts at: 3465 4423 442
Size: 958 1753
Fragments arranged by size:
1753 958 914 655 479 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2081 1056
Size: 1025 4709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2412 2555 4394 1239
Size: 1173 143 1839 2579
Fragments arranged by size:
2579 1839 1173 143
Bpu10I CC'TnA_GC
Cuts at: 2405 2405
Size: 5734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2657 2864 3859 4724 5022 2657
Size: 207 995 865 298 3369
Fragments arranged by size:
3369 995 865 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4397 882
Size: 617 2898 2219
Fragments arranged by size:
2898 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3525 493
Size: 711 2321 2702
Fragments arranged by size:
2702 2321 711
BsaBI GATnn'nnATC
Cuts at: 5728 5728
Size: 5734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2656 3541 3923 4558
Size: 53 83 186 936 1123 885 382 635
Cuts at: 4558 275
Size: 1451
Fragments arranged by size:
1451 1123 936 885 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2493 4167 4565 4839 4986 5397 2493
Size: 1674 398 274 147 411 2830
Fragments arranged by size:
2830 1674 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2528 2558 1178
Size: 30 165 30 1125 30 4354
Fragments arranged by size:
4354 1125 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2335 1186
Size: 1149 4585
BseYI C'CCAG_C
Cuts at: 2247 2826 2889 4937 5433 2247
Size: 579 63 2048 496 2548
Fragments arranged by size:
2548 2048 579 496 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2730 1163
Size: 573 994 4167
Fragments arranged by size:
4167 994 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3184 3945 4973 1540
Size: 275 186 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2399 3795 3880 4951 2399
Size: 1396 85 1071 3182
Fragments arranged by size:
3182 1396 1071 85
BsmI GAATG_Cn'
Cuts at: 2097 3245 3338 2097
Size: 1148 93 4493
Fragments arranged by size:
4493 1148 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2399 2772 3053 3795 3880 4951
Size: 399 235 374 373 281 742 85 1071
Cuts at: 4951 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 374 373 281 235
85
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 3623 5353 3623
Size: 1730 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3089 844
Size: 2245 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3621 1394
Size: 429 1798 3507
Fragments arranged by size:
3507 1798 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4229 4403 65
Size: 1183 2981 174 1396
Fragments arranged by size:
2981 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 2486 4378 4565 1700
Size: 110 227 449 1892 187 2869
Fragments arranged by size:
2869 1892 449 227 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5734
BssSI C'ACGA_G
Cuts at: 3794 4806 3794
Size: 1012 4722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2071 794
Size: 1277 4457
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 2409 1184
Size: 1225 4509
BstYI r'GATC_y
Cuts at: 1972 2115 2148 3064 3431 3816 3833 5285 5371
Size: 143 33 916 367 385 17 1452 86
Cuts at: 5371 5383 5729 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 916 385 367 346 143 86
33 17 12
Bsu36I CC'TnA_GG
Cuts at: 2610 2610
Size: 5734
BtgI C'CryG_G
Cuts at: 513 1065 2210 2578 513
Size: 552 1145 368 3669
Fragments arranged by size:
3669 1145 552 368
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4995
Fragments arranged by size:
4995 683 56
BtsI GCAGTG_nn'
Cuts at: 2770 3250 4044 4064 2770
Size: 480 794 20 4440
Fragments arranged by size:
4440 794 480 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5699
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2488 2970
Size: 54 1425 50 275 18 205 453 482
Cuts at: 2970 3181 4070 5699 8
Size: 211 889 1629 43
Fragments arranged by size:
1629 1425 889 482 453 275 211 205
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 1537
Size: 275 1369 4090
Fragments arranged by size:
4090 1369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2638 3664 1353
Size: 1285 1026 3423
Fragments arranged by size:
3423 1285 1026
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4321 4695 4841 387
Size: 1064 138 2732 374 146 1280
Fragments arranged by size:
2732 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2328 2412 2555 3797 4394 5181
Size: 393 641 55 84 143 1242 597 787
Cuts at: 5181 5449 1239
Size: 268 1524
Fragments arranged by size:
1524 1242 787 641 597 393 268 143
84 55
EcoO109I rG'GnC_Cy
Cuts at: 2804 2992 2804
Size: 188 5546
EcoRI G'AATT_C
Cuts at: 2914 3043 3421 2914
Size: 129 378 5227
Fragments arranged by size:
5227 378 129
FseI GG_CCGG'CC
Cuts at: 2041 2041
Size: 5734
FspI TGC'GCA
Cuts at: 4240 4240
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 4881 1678
Size: 3203 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4375 4407 4449 4481 1373
Size: 32 135 32 2803 32 42 32 2626
Fragments arranged by size:
2803 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 3324 669
Size: 988 281 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KpnI G_GTAC'C
Cuts at: 2475 3059 2475
Size: 584 5150
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2314 2661 4848 5032 1305
Size: 1009 347 2187 184 2007
Fragments arranged by size:
2187 2007 1009 347 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2972 10
Size: 54 1425 343 1140 2772
Fragments arranged by size:
2772 1425 1140 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3692 4051 4210 518
Size: 648 2526 359 159 2042
Fragments arranged by size:
2526 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2251 2417 2432 2689 2821 3150 3827 4975
Size: 645 166 15 257 132 329 677 1148
Cuts at: 4975 5220 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 645 329 257 245 166
132 15
NaeI GCC'GGC
Cuts at: 2039 2039
Size: 5734
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5182
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NgoMIV G'CCGG_C
Cuts at: 2037 2037
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 2077 1052
Size: 1025 4709
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 3092 4637 1132
Size: 97 1863 1545 2229
Fragments arranged by size:
2229 1863 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2480 2704 1348
Size: 1132 224 4378
Fragments arranged by size:
4378 1132 224
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5734
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2528 2560 3803 3835 5316 5348 2528
Size: 32 1243 32 1481 32 2914
Fragments arranged by size:
2914 1481 1243 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2804 2804
Size: 5734
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5734
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PstI C_TGCA'G
Cuts at: 830 3086 830
Size: 2256 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
PvuII CAG'CTG
Cuts at: 2251 2417 2432 2689 2821 2251
Size: 166 15 257 132 5164
Fragments arranged by size:
5164 257 166 132 15
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SanDI GG'GwC_CC
Cuts at: 2804 2804
Size: 5734
SapI GCTCTTCn'nnn_
Cuts at: 2638 2638
Size: 5734
SbfI CC_TGCA'GG
Cuts at: 3086 3086
Size: 5734
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 3082 4217 4898 5089 826
Size: 219 2037 1135 681 191 1471
Fragments arranged by size:
2037 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 2468 3061 1872
Size: 596 593 4545
Fragments arranged by size:
4545 596 593
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 2672 2879 3838 4739 5001
Size: 197 926 177 552 207 959 901 262
Cuts at: 5001 820
Size: 1553
Fragments arranged by size:
1553 959 926 901 552 262 207 197
177
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 3092 1132
Size: 1960 3774
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5734
StyI C'CwwG_G
Cuts at: 513 1065 2152 2720 3173 513
Size: 552 1087 568 453 3074
Fragments arranged by size:
3074 1087 568 552 453
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2336 2493 3761 3778 3931 4116 4561
Size: 1255 756 157 1268 17 153 185 445
Cuts at: 4561 325
Size: 1498
Fragments arranged by size:
1498 1268 1255 756 445 185 157 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3980 5692 96
Size: 274 80 33 51 1443 2003 1712 138
Fragments arranged by size:
2003 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5694 5726 5694
Size: 32 5702
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5734
XmaI C'CCGG_G
Cuts at: 1870 2466 3059 1870
Size: 596 593 4545
Fragments arranged by size:
4545 596 593
XmnI GAAnn'nnTTC
Cuts at: 2543 3863 2543
Size: 1320 4414
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SexAI SfiI SfoI SrfI SwaI XcmI