Restriction map for
pFN21AE0919
(Circular) (Six-base) MAPSORT of: pFN21AE0919.seq Check: 976 from: 1 to: 5620
pFN21AE0919 5620 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2612 2626 2797 2612
Size: 14 171 5435
Fragments arranged by size:
5435 171 14
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2963 1937
Size: 1026 4594
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3683 5067 5335 1632
Size: 2051 1384 268 1917
Fragments arranged by size:
2051 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 2337 4519 5496 1203
Size: 1134 2182 977 1327
Fragments arranged by size:
2182 1327 1134 977
AgeI A'CCGG_T
Cuts at: 2703 4451 2703
Size: 1748 3872
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2187 1184
Size: 1003 4617
AlwNI CAG_nnn'CTG
Cuts at: 3363 4935 3363
Size: 1572 4048
ApaI G_GGCC'C
Cuts at: 2078 2223 2078
Size: 145 5475
ApaLI G'TGCA_C
Cuts at: 2360 3677 4833 2360
Size: 1317 1156 3147
Fragments arranged by size:
3147 1317 1156
ApoI r'AATT_y
Cuts at: 1493 2435 2929 3155 3307 5416 1493
Size: 942 494 226 152 2109 1697
Fragments arranged by size:
2109 1697 942 494 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 2585 2945 1870
Size: 73 127 515 360 4545
Fragments arranged by size:
4545 515 360 127 73
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2248 2941 4396 618
Size: 325 217 627 461 693 1455 1842
Fragments arranged by size:
1842 1455 693 627 461 325 217
BanII G_rGCy'C
Cuts at: 1391 2078 2223 2585 2890 2939 1391
Size: 687 145 362 305 49 4072
Fragments arranged by size:
4072 687 362 305 145 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4649
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2047 2081 3811 3845 1903
Size: 34 110 34 1730 34 3678
Fragments arranged by size:
3678 1730 110 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 2595 2727 1548
Size: 1047 132 4441
Fragments arranged by size:
4441 1047 132
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 2889 3037 2889
Size: 148 5472
Bme1580I G_kGCm'C
Cuts at: 1790 2078 2223 2364 3681 4837 1790
Size: 288 145 141 1317 1156 2573
Fragments arranged by size:
2573 1317 1156 288 145 141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3351 4309 442
Size: 416 53 83 399 24 1934 958 1753
Fragments arranged by size:
1934 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2551 2807 4280 1239
Size: 1097 215 256 1473 2579
Fragments arranged by size:
2579 1473 1097 256 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2444 2732 3745 4610 4908 2444
Size: 288 1013 865 298 3156
Fragments arranged by size:
3156 1013 865 298 288
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2569 2828 4283 882
Size: 617 1070 259 1455 2219
Fragments arranged by size:
2219 1455 1070 617 259
BsaAI yAC'GTr
Cuts at: 493 1204 2340 3411 493
Size: 711 1136 1071 2702
Fragments arranged by size:
2702 1136 1071 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2703 4053 4451 4725 4872 5283 2703
Size: 1350 398 274 147 411 3040
Fragments arranged by size:
3040 1350 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5395
Fragments arranged by size:
5395 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5620
BseYI C'CCAG_C
Cuts at: 2086 4823 5319 2086
Size: 2737 496 2387
Fragments arranged by size:
2737 2387 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2007 1163
Size: 573 271 4776
Fragments arranged by size:
4776 573 271
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2083 3070 3831 4859 1540
Size: 275 186 82 987 761 1028 2301
Fragments arranged by size:
2301 1028 987 761 275 186 82
BsiHKAI G_wGCw'C
Cuts at: 2364 2883 3681 3766 4837 2364
Size: 519 798 85 1071 3147
Fragments arranged by size:
3147 1071 798 519 85
BsmI GAATG_Cn'
Cuts at: 3131 3224 3131
Size: 93 5527
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2223 2364 2585 2883 2890 2939
Size: 399 288 145 141 221 298 7 49
Cuts at: 2939 3681 3766 4837 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 298 288 221 145
141 85 49 7
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2612 2626 2797 2975 844
Size: 1768 14 171 178 3489
Fragments arranged by size:
3489 1768 178 171 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2713 3507 1394
Size: 429 890 794 3507
Fragments arranged by size:
3507 890 794 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2847 4115 4289 65
Size: 1183 1599 1268 174 1396
Fragments arranged by size:
1599 1396 1268 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2703 4264 4451 1700
Size: 110 893 1561 187 2869
Fragments arranged by size:
2869 1561 893 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 2472 4424 2472
Size: 1952 3668
BstEII G'GTnAC_C
Cuts at: 1184 2812 1184
Size: 1628 3992
BstYI r'GATC_y
Cuts at: 1972 2464 2950 3317 3702 3719 5171 5257 5269
Size: 492 486 367 385 17 1452 86 12
Cuts at: 5269 5615 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 492 486 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 2255 2409 513
Size: 552 939 36 215 154 3724
Fragments arranged by size:
3724 939 552 215 154 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2214 487
Size: 683 56 988 3893
Fragments arranged by size:
3893 988 683 56
BtsI GCAGTG_nn'
Cuts at: 3136 3930 3950 3136
Size: 794 20 4806
Fragments arranged by size:
4806 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2274 2368
Size: 54 1425 50 275 18 250 194 94
Cuts at: 2368 3067 3956 5585 8
Size: 699 889 1629 43
Fragments arranged by size:
1629 1425 889 699 275 250 194 94
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2080 3067 1537
Size: 275 268 987 4090
Fragments arranged by size:
4090 987 275 268
EarI CTCTTCn'nnn_
Cuts at: 1353 2474 3550 1353
Size: 1121 1076 3423
Fragments arranged by size:
3423 1121 1076
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2026 2408 4207 4581 4727 387
Size: 1064 138 437 382 1799 374 146 1280
Fragments arranged by size:
1799 1280 1064 437 382 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2551 2807 3683 4280 5067 5335
Size: 393 704 215 256 876 597 787 268
Cuts at: 5335 1239
Size: 1524
Fragments arranged by size:
1524 876 787 704 597 393 268 256
215
EcoNI CCTnn'n_nnAGG
Cuts at: 2196 2196
Size: 5620
EcoO109I rG'GnC_Cy
Cuts at: 2074 2075 2219 2329 2480 2074
Size: 1 144 110 151 5214
Fragments arranged by size:
5214 151 144 110 1
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 5620
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4261 4293 4335 4367 1373
Size: 32 135 32 2689 32 42 32 2626
Fragments arranged by size:
2689 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2277 4734 4918 1305
Size: 972 2457 184 2007
Fragments arranged by size:
2457 2007 972 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2276 2370 10
Size: 54 1425 343 444 94 3260
Fragments arranged by size:
3260 1425 444 343 94 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2254 3578 3937 4096 518
Size: 648 1088 1324 359 159 2042
Fragments arranged by size:
2042 1324 1088 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2188 3036 3713 4861 5106 1606
Size: 582 848 677 1148 245 2120
Fragments arranged by size:
2120 1148 848 677 582 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2255 513
Size: 552 939 251 3878
Fragments arranged by size:
3878 939 552 251
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2978 4523 1132
Size: 97 1749 1545 2229
Fragments arranged by size:
2229 1749 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2289 1108
Size: 1181 4439
PfoI T'CCnGG_A
Cuts at: 2313 2439 2313
Size: 126 5494
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2329 2480 2329
Size: 151 5469
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PspOMI G'GGCC_C
Cuts at: 2074 2219 2074
Size: 145 5475
PstI C_TGCA'G
Cuts at: 830 2218 2808 2972 830
Size: 1388 590 164 3478
Fragments arranged by size:
3478 1388 590 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SanDI GG'GwC_CC
Cuts at: 2480 2480
Size: 5620
SbfI CC_TGCA'GG
Cuts at: 2218 2808 2972 2218
Size: 590 164 4866
Fragments arranged by size:
4866 590 164
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2214 2386 2804 2902 2968 4103 4784
Size: 219 1169 172 418 98 66 1135 681
Cuts at: 4784 4975 826
Size: 191 1471
Fragments arranged by size:
1471 1169 1135 681 418 219 191 172
98 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2072 2947 1872
Size: 200 875 4545
Fragments arranged by size:
4545 875 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2459 2747 3724 4625 4887 820
Size: 197 926 516 288 977 901 262 1553
Fragments arranged by size:
1553 977 926 901 516 288 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5620
StyI C'CwwG_G
Cuts at: 513 1065 2004 2255 3059 513
Size: 552 939 251 804 3074
Fragments arranged by size:
3074 939 804 552 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3647 3664 3817 4002 4447 325
Size: 1255 2067 17 153 185 445 1498
Fragments arranged by size:
2067 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2721 2766 3866
Size: 274 80 33 51 1443 744 45 1100
Cuts at: 3866 5578 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1100 744 274 138 80 51
45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2806 2806
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2070 2945 1870
Size: 200 875 4545
Fragments arranged by size:
4545 875 200
XmnI GAAnn'nnTTC
Cuts at: 2153 3749 2153
Size: 1596 4024
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI