Restriction map for
pFN21AE0916
(Circular) (Six-base) MAPSORT of: pFN21AE0916.seq Check: 4105 from: 1 to: 5566
pFN21AE0916 5566 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3376 278
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2331 2909 1937
Size: 394 578 4594
Fragments arranged by size:
4594 578 394
Acc65I G'GTAC_C
Cuts at: 2887 2887
Size: 5566
AclI AA'CG_TT
Cuts at: 1563 3693 4066 1563
Size: 2130 373 3063
Fragments arranged by size:
3063 2130 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3629 5013 5281 1632
Size: 1997 1384 268 1917
Fragments arranged by size:
1997 1917 1384 268
AfeI AGC'GCT
Cuts at: 2390 2390
Size: 5566
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5369
AflIII A'CryG_T
Cuts at: 1203 4465 5442 1203
Size: 3262 977 1327
Fragments arranged by size:
3262 1327 977
AgeI A'CCGG_T
Cuts at: 4397 4397
Size: 5566
AhdI GACnn_n'nnGTC
Cuts at: 4295 4295
Size: 5566
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5566
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5566
AlwNI CAG_nnn'CTG
Cuts at: 2530 3309 4881 2530
Size: 779 1572 3215
Fragments arranged by size:
3215 1572 779
ApaI G_GGCC'C
Cuts at: 2495 2495
Size: 5566
ApaLI G'TGCA_C
Cuts at: 3623 4779 3623
Size: 1156 4410
ApoI r'AATT_y
Cuts at: 1493 2875 3101 3253 5362 1493
Size: 1382 226 152 2109 1697
Fragments arranged by size:
2109 1697 1382 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4120 160
Size: 872 3088 1606
Fragments arranged by size:
3088 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
AvaI C'yCGr_G
Cuts at: 1870 1943 2519 2638 2722 2891 1870
Size: 73 576 119 84 169 4545
Fragments arranged by size:
4545 576 169 119 84 73
BamHI G'GATC_C
Cuts at: 2896 3263 2896
Size: 367 5199
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2887 4342 618
Size: 325 217 627 1100 1455 1842
Fragments arranged by size:
1842 1455 1100 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2478 2495 2885 1391
Size: 1087 17 390 4072
Fragments arranged by size:
4072 1087 390 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4595
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3757 3791 1903
Size: 34 50 34 1736 34 3678
Fragments arranged by size:
3678 1736 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3458 4668 3458
Size: 1210 4356
BclI T'GATC_A
Cuts at: 1548 2175 1548
Size: 627 4939
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4177 136
Size: 107 122 71 631 605 2505 1525
Fragments arranged by size:
2505 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5561 5561
Size: 5566
BlpI GC'TnA_GC
Cuts at: 2189 2983 2189
Size: 794 4772
Bme1580I G_kGCm'C
Cuts at: 1790 2495 3627 4783 1790
Size: 705 1132 1156 2573
Fragments arranged by size:
2573 1156 1132 705
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5566
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3297 4255 442
Size: 416 53 83 399 24 1880 958 1753
Fragments arranged by size:
1880 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2489 2792 2848 4226 1239
Size: 902 348 303 56 1378 2579
Fragments arranged by size:
2579 1378 902 348 303 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3691 4556 4854 3691
Size: 865 298 4403
Fragments arranged by size:
4403 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4229 882
Size: 617 2730 2219
Fragments arranged by size:
2730 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2073 2181 2655 3357 493
Size: 711 869 108 474 702 2702
Fragments arranged by size:
2702 869 711 702 474 108
BsaBI GATnn'nnATC
Cuts at: 2138 5560 2138
Size: 3422 2144
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3373 3755 4390 275
Size: 53 83 186 936 1840 382 635 1451
Fragments arranged by size:
1840 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3999 4397 4671 4818 5229 3999
Size: 398 274 147 411 4336
Fragments arranged by size:
4336 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2108 2138 1178
Size: 30 165 30 705 30 4606
Fragments arranged by size:
4606 705 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2260 2486 1186
Size: 1074 226 4266
Fragments arranged by size:
4266 1074 226
BseYI C'CCAG_C
Cuts at: 2379 2608 2627 4769 5265 2379
Size: 229 19 2142 496 2680
Fragments arranged by size:
2680 2142 496 229 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2053 2193 2482 1163
Size: 573 317 140 289 4247
Fragments arranged by size:
4247 573 317 289 140
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2356 3016 3777 4805 1540
Size: 275 186 355 660 761 1028 2301
Fragments arranged by size:
2301 1028 761 660 355 275 186
BsiHKAI G_wGCw'C
Cuts at: 2297 3627 3712 4783 2297
Size: 1330 85 1071 3080
Fragments arranged by size:
3080 1330 1071 85
BsmI GAATG_Cn'
Cuts at: 3077 3170 3077
Size: 93 5473
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2297 2478 2495 2885 3627 3712 4783
Size: 399 507 181 17 390 742 85 1071
Cuts at: 4783 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 507 399 390 181 85
17
BspEI T'CCGG_A
Cuts at: 5229 5229
Size: 5566
BspHI T'CATG_A
Cuts at: 3455 5185 3455
Size: 1730 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2317 2430 2448 2921 844
Size: 1473 113 18 473 3489
Fragments arranged by size:
3489 1473 473 113 18
BsrBI CCG'CTC
Cuts at: 1394 1823 3453 1394
Size: 429 1630 3507
Fragments arranged by size:
3507 1630 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4061 4235 65
Size: 1183 2813 174 1396
Fragments arranged by size:
2813 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2226 4210 4397 1700
Size: 110 416 1984 187 2869
Fragments arranged by size:
2869 1984 416 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5566
BssHII G'CGCG_C
Cuts at: 1925 2554 1925
Size: 629 4937
BssSI C'ACGA_G
Cuts at: 2429 3626 4638 2429
Size: 1197 1012 3357
Fragments arranged by size:
3357 1197 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5566
BstBI TT'CG_AA
Cuts at: 4370 4370
Size: 5566
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5566
BstYI r'GATC_y
Cuts at: 1972 2896 3263 3648 3665 5117 5203 5215 5561
Size: 924 367 385 17 1452 86 12 346
Cuts at: 5561 1972
Size: 1977
Fragments arranged by size:
1977 1452 924 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5014
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2785 487
Size: 683 56 1559 3268
Fragments arranged by size:
3268 1559 683 56
BtsI GCAGTG_nn'
Cuts at: 2758 3082 3876 3896 2758
Size: 324 794 20 4428
Fragments arranged by size:
4428 794 324 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5531
DraI TTT'AAA
Cuts at: 2870 2870
Size: 5566
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4573 809
Size: 507 3257 1802
Fragments arranged by size:
3257 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 2713 3013
Size: 54 1425 50 275 18 531 352 300
Cuts at: 3013 3902 5531 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 531 352 300 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3013 1537
Size: 275 1201 4090
Fragments arranged by size:
4090 1201 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3496 1353
Size: 2143 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2257 4153 4527 4673 387
Size: 1064 138 668 1896 374 146 1280
Fragments arranged by size:
1896 1280 1064 668 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2489 2792 2848 3629 4226 5013
Size: 393 509 348 303 56 781 597 787
Cuts at: 5013 5281 1239
Size: 268 1524
Fragments arranged by size:
1524 787 781 597 509 393 348 303
268 56
EcoO109I rG'GnC_Cy
Cuts at: 2491 2492 2515 2727 2778 2491
Size: 1 23 212 51 5279
Fragments arranged by size:
5279 212 51 23 1
EcoRI G'AATT_C
Cuts at: 2875 3253 2875
Size: 378 5188
FspI TGC'GCA
Cuts at: 4072 4072
Size: 5566
HaeII r_GCGC'y
Cuts at: 1678 2392 2652 4713 1678
Size: 714 260 2061 2531
Fragments arranged by size:
2531 2061 714 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 2847 2879 4207
Size: 32 135 32 674 32 569 32 1328
Cuts at: 4207 4239 4281 4313 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1328 674 569 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2026 2910 3156 669
Size: 988 281 88 884 246 3079
Fragments arranged by size:
3079 988 884 281 246 88
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5566
HpaI GTT'AAC
Cuts at: 3156 3156
Size: 5566
KpnI G_GTAC'C
Cuts at: 2891 2891
Size: 5566
MfeI C'AATT_G
Cuts at: 3165 3165
Size: 5566
MluI A'CGCG_T
Cuts at: 5442 5442
Size: 5566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4680 4864 1305
Size: 3375 184 2007
Fragments arranged by size:
3375 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3744
Fragments arranged by size:
3744 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2147 3524 3883 4042 518
Size: 648 978 3 1377 359 159 2042
Fragments arranged by size:
2042 1377 978 648 359 159 3
MspA1I CmG'CkG
Cuts at: 1606 2383 2530 2667 2982 3659 4807 5052 1606
Size: 777 147 137 315 677 1148 245 2120
Fragments arranged by size:
2120 1148 777 677 315 245 147 137
NaeI GCC'GGC
Cuts at: 2228 2228
Size: 5566
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5014
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5566
NgoMIV G'CCGG_C
Cuts at: 2226 2226
Size: 5566
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5566
NotI GC'GGCC_GC
Cuts at: 3013 3013
Size: 5566
NspI r_CATG'y
Cuts at: 1132 1229 2924 4469 1132
Size: 97 1695 1545 2229
Fragments arranged by size:
2229 1695 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5566
PciI A'CATG_T
Cuts at: 4465 4465
Size: 5566
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2104 1108
Size: 996 4570
PmeI GTTT'AAAC
Cuts at: 2870 2870
Size: 5566
PmlI CAC'GTG
Cuts at: 2073 2181 2655 2073
Size: 108 474 4984
Fragments arranged by size:
4984 474 108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 5148 5180 3635
Size: 32 1481 32 4021
Fragments arranged by size:
4021 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2515 2727 2778 2515
Size: 212 51 5303
Fragments arranged by size:
5303 212 51
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5566
PsiI TTA'TAA
Cuts at: 3136 3136
Size: 5566
PspOMI G'GGCC_C
Cuts at: 2491 2491
Size: 5566
PstI C_TGCA'G
Cuts at: 830 2427 2918 830
Size: 1597 491 3478
Fragments arranged by size:
3478 1597 491
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5566
PvuII CAG'CTG
Cuts at: 2530 2530
Size: 5566
SalI G'TCGA_C
Cuts at: 1936 2908 1936
Size: 972 4594
SanDI GG'GwC_CC
Cuts at: 2515 2778 2515
Size: 263 5303
SbfI CC_TGCA'GG
Cuts at: 2918 2918
Size: 5566
ScaI AGT'ACT
Cuts at: 3814 5526 3814
Size: 1712 3854
SfcI C'TryA_G
Cuts at: 826 1045 2423 2698 2914 4049 4730 4921 826
Size: 219 1378 275 216 1135 681 191 1471
Fragments arranged by size:
1471 1378 1135 681 275 219 216 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5566
SmaI CCC'GGG
Cuts at: 1872 2724 2893 1872
Size: 852 169 4545
Fragments arranged by size:
4545 852 169
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3670 4571 4833 820
Size: 197 926 1727 901 262 1553
Fragments arranged by size:
1727 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3357 493
Size: 2864 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5566
SphI G_CATG'C
Cuts at: 1132 2924 1132
Size: 1792 3774
SspI AAT'ATT
Cuts at: 5 52 3490 5
Size: 47 3438 2081
Fragments arranged by size:
3438 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5566
StyI C'CwwG_G
Cuts at: 513 1065 3005 513
Size: 552 1940 3074
Fragments arranged by size:
3074 1940 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3593 3610 3763 3948 4393 325
Size: 1255 2013 17 153 185 445 1498
Fragments arranged by size:
2013 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3812 5524 96
Size: 274 80 33 51 1443 1835 1712 138
Fragments arranged by size:
1835 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5526 5558 5526
Size: 32 5534
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5566
XbaI T'CTAG_A
Cuts at: 2902 2902
Size: 5566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2356 2750 2356
Size: 394 5172
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5566
XmaI C'CCGG_G
Cuts at: 1870 2722 2891 1870
Size: 852 169 4545
Fragments arranged by size:
4545 852 169
XmnI GAAnn'nnTTC
Cuts at: 3695 3695
Size: 5566
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3374 276
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI