Restriction map for
pFN21AE0915
(Circular) (Six-base) MAPSORT of: pFN21AE0915.seq Check: 1152 from: 1 to: 5494
pFN21AE0915 5494 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3304 278
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2837 1937
Size: 900 4594
Acc65I G'GTAC_C
Cuts at: 2815 2815
Size: 5494
AclI AA'CG_TT
Cuts at: 1563 3621 3994 1563
Size: 2058 373 3063
Fragments arranged by size:
3063 2058 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3557 4941 5209 1632
Size: 1925 1384 268 1917
Fragments arranged by size:
1925 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5297
AflIII A'CryG_T
Cuts at: 1203 4393 5370 1203
Size: 3190 977 1327
Fragments arranged by size:
3190 1327 977
AgeI A'CCGG_T
Cuts at: 2038 4325 2038
Size: 2287 3207
AhdI GACnn_n'nnGTC
Cuts at: 4223 4223
Size: 5494
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2350 1184
Size: 1166 4328
AlwNI CAG_nnn'CTG
Cuts at: 2343 3237 4809 2343
Size: 894 1572 3028
Fragments arranged by size:
3028 1572 894
ApaLI G'TGCA_C
Cuts at: 3551 4707 3551
Size: 1156 4338
ApoI r'AATT_y
Cuts at: 1493 2803 3029 3181 5290 1493
Size: 1310 226 152 2109 1697
Fragments arranged by size:
2109 1697 1310 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4048 160
Size: 872 3016 1606
Fragments arranged by size:
3016 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
AvaI C'yCGr_G
Cuts at: 1870 1943 2819 1870
Size: 73 876 4545
Fragments arranged by size:
4545 876 73
BamHI G'GATC_C
Cuts at: 2733 2824 3191 2733
Size: 91 367 5036
Fragments arranged by size:
5036 367 91
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2309 2474 2815 4270 618
Size: 325 217 627 522 165 341 1455 1842
Fragments arranged by size:
1842 1455 627 522 341 325 217 165
BanII G_rGCy'C
Cuts at: 1391 2813 1391
Size: 1422 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3685 3719 1903
Size: 34 1748 34 3678
Fragments arranged by size:
3678 1748 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3386 4596 3386
Size: 1210 4284
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5494
BfrBI ATG'CAT
Cuts at: 2585 2585
Size: 5494
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4105 136
Size: 107 122 71 631 605 2433 1525
Fragments arranged by size:
2433 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2080 5489 2080
Size: 3409 2085
BlpI GC'TnA_GC
Cuts at: 2911 2911
Size: 5494
Bme1580I G_kGCm'C
Cuts at: 1790 2477 3555 4711 1790
Size: 687 1078 1156 2573
Fragments arranged by size:
2573 1156 1078 687
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5494
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3225 4183 442
Size: 416 53 83 399 24 1808 958 1753
Fragments arranged by size:
1808 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2218 2728 4154 1239
Size: 979 510 1426 2579
Fragments arranged by size:
2579 1426 979 510
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3619 4484 4782 3619
Size: 865 298 4331
Fragments arranged by size:
4331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2023 4157 882
Size: 617 524 2134 2219
Fragments arranged by size:
2219 2134 617 524
BsaAI yAC'GTr
Cuts at: 493 1204 2521 2716 3285 493
Size: 711 1317 195 569 2702
Fragments arranged by size:
2702 1317 711 569 195
BsaBI GATnn'nnATC
Cuts at: 2288 5488 2288
Size: 3200 2294
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3301 3683 4318 275
Size: 53 83 186 936 1768 382 635 1451
Fragments arranged by size:
1768 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2038 2538 3927 4325 4599 4746 5157 2038
Size: 500 1389 398 274 147 411 2375
Fragments arranged by size:
2375 1389 500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2095 2125 2205 2235 2246
Size: 30 165 30 692 30 80 30 11
Cuts at: 2246 2276 2685 2715 1178
Size: 30 409 30 3957
Fragments arranged by size:
3957 692 409 165 80 30 30 30
30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5494
BseYI C'CCAG_C
Cuts at: 2139 2318 2490 4697 5193 2139
Size: 179 172 2207 496 2440
Fragments arranged by size:
2440 2207 496 179 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2097 2718 1163
Size: 573 361 621 3939
Fragments arranged by size:
3939 621 573 361
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2944 3705 4733 1540
Size: 275 186 943 761 1028 2301
Fragments arranged by size:
2301 1028 943 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3555 3640 4711 3555
Size: 85 1071 4338
Fragments arranged by size:
4338 1071 85
BsmI GAATG_Cn'
Cuts at: 3005 3098 3005
Size: 93 5401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2477 2813 3555 3640 4711 1391
Size: 399 687 336 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 687 399 336 85
BspEI T'CCGG_A
Cuts at: 5157 5157
Size: 5494
BspHI T'CATG_A
Cuts at: 3383 5113 3383
Size: 1730 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2310 2849 844
Size: 1466 539 3489
Fragments arranged by size:
3489 1466 539
BsrBI CCG'CTC
Cuts at: 1394 1823 3381 1394
Size: 429 1558 3507
Fragments arranged by size:
3507 1558 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2256 2372 3989 4163 65
Size: 1183 1008 116 1617 174 1396
Fragments arranged by size:
1617 1396 1183 1008 174 116
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 4138 4325 1700
Size: 110 228 2100 187 2869
Fragments arranged by size:
2869 2100 228 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5494
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5494
BssSI C'ACGA_G
Cuts at: 3554 4566 3554
Size: 1012 4482
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5494
BstBI TT'CG_AA
Cuts at: 2386 4298 2386
Size: 1912 3582
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5494
BstXI CCAn_nnnn'nTGG
Cuts at: 2023 2023
Size: 5494
BstYI r'GATC_y
Cuts at: 1972 2080 2089 2733 2824 3191 3576 3593 5045
Size: 108 9 644 91 367 385 17 1452
Cuts at: 5045 5131 5143 5489 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 644 385 367 346 108 91
86 17 12 9
BtgI C'CryG_G
Cuts at: 513 1065 2004 2209 2313 2469 513
Size: 552 939 205 104 156 3538
Fragments arranged by size:
3538 939 552 205 156 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4755
Fragments arranged by size:
4755 683 56
BtsI GCAGTG_nn'
Cuts at: 3010 3804 3824 3010
Size: 794 20 4680
Fragments arranged by size:
4680 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5494
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5459
DraI TTT'AAA
Cuts at: 2798 2798
Size: 5494
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2070 4501 809
Size: 507 754 2431 1802
Fragments arranged by size:
2431 1802 754 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2941 3830
Size: 54 1425 50 275 18 297 814 889
Cuts at: 3830 5459 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 814 297 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2941 1537
Size: 275 1129 4090
Fragments arranged by size:
4090 1129 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2148 3424 1353
Size: 795 1276 3423
Fragments arranged by size:
3423 1276 795
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2195 4081 4455 4601 387
Size: 1064 138 606 1886 374 146 1280
Fragments arranged by size:
1886 1280 1064 606 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2218 2728 3557 4154 4941 5209 1239
Size: 393 586 510 829 597 787 268 1524
Fragments arranged by size:
1524 829 787 597 586 510 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2504 2504
Size: 5494
EcoRI G'AATT_C
Cuts at: 2803 3181 2803
Size: 378 5116
FspI TGC'GCA
Cuts at: 4000 4000
Size: 5494
HaeII r_GCGC'y
Cuts at: 1678 2012 4641 1678
Size: 334 2629 2531
Fragments arranged by size:
2629 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2672 2685 2704 2717 4135
Size: 32 135 32 1100 13 19 13 1418
Cuts at: 4135 4167 4209 4241 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1418 1100 135 42 32 32 32
32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2704 2838 3084 669
Size: 988 281 766 134 246 3079
Fragments arranged by size:
3079 988 766 281 246 134
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5494
HpaI GTT'AAC
Cuts at: 3084 3084
Size: 5494
KpnI G_GTAC'C
Cuts at: 2819 2819
Size: 5494
MfeI C'AATT_G
Cuts at: 3093 3093
Size: 5494
MluI A'CGCG_T
Cuts at: 5370 5370
Size: 5494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2004 4608 4792 1305
Size: 699 2604 184 2007
Fragments arranged by size:
2604 2007 699 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3672
Fragments arranged by size:
3672 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3452 3811 3970 518
Size: 648 2286 359 159 2042
Fragments arranged by size:
2286 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2228 2246 2330 2414 2910 3587 4735 4980
Size: 622 18 84 84 496 677 1148 245
Cuts at: 4980 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 622 496 245 84 84
18
NcoI C'CATG_G
Cuts at: 513 1065 2004 2209 2313 2469 513
Size: 552 939 205 104 156 3538
Fragments arranged by size:
3538 939 552 205 156 104
NdeI CA'TA_TG
Cuts at: 387 2587 387
Size: 2200 3294
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5494
NotI GC'GGCC_GC
Cuts at: 2941 2941
Size: 5494
NsiI A_TGCA'T
Cuts at: 2587 2587
Size: 5494
NspI r_CATG'y
Cuts at: 1132 1229 2852 4397 1132
Size: 97 1623 1545 2229
Fragments arranged by size:
2229 1623 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5494
PciI A'CATG_T
Cuts at: 4393 4393
Size: 5494
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5494
PmeI GTTT'AAAC
Cuts at: 2798 2798
Size: 5494
PmlI CAC'GTG
Cuts at: 2716 2716
Size: 5494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 5076 5108 3563
Size: 32 1481 32 3949
Fragments arranged by size:
3949 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5494
PsiI TTA'TAA
Cuts at: 3064 3064
Size: 5494
PstI C_TGCA'G
Cuts at: 830 2846 830
Size: 2016 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5494
PvuII CAG'CTG
Cuts at: 2228 2414 2228
Size: 186 5308
SalI G'TCGA_C
Cuts at: 1936 2836 1936
Size: 900 4594
SapI GCTCTTCn'nnn_
Cuts at: 2148 2148
Size: 5494
SbfI CC_TGCA'GG
Cuts at: 2846 2846
Size: 5494
ScaI AGT'ACT
Cuts at: 3742 5454 3742
Size: 1712 3782
SfcI C'TryA_G
Cuts at: 826 1045 2241 2406 2562 2674 2842 3977 4658
Size: 219 1196 165 156 112 168 1135 681
Cuts at: 4658 4849 826
Size: 191 1471
Fragments arranged by size:
1471 1196 1135 681 219 191 168 165
156 112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5494
SmaI CCC'GGG
Cuts at: 1872 2821 1872
Size: 949 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3598 4499 4761 820
Size: 197 926 1655 901 262 1553
Fragments arranged by size:
1655 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3285 493
Size: 2792 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5494
SphI G_CATG'C
Cuts at: 1132 2852 1132
Size: 1720 3774
SspI AAT'ATT
Cuts at: 5 52 3418 5
Size: 47 3366 2081
Fragments arranged by size:
3366 2081 47
StuI AGG'CCT
Cuts at: 1517 2636 1517
Size: 1119 4375
StyI C'CwwG_G
Cuts at: 513 1065 2004 2209 2313 2469 2933 513
Size: 552 939 205 104 156 464 3074
Fragments arranged by size:
3074 939 552 464 205 156 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3521 3538 3691 3876 4321 325
Size: 1255 1941 17 153 185 445 1498
Fragments arranged by size:
1941 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2448 3740 5452
Size: 274 80 33 51 1443 471 1292 1712
Cuts at: 5452 96
Size: 138
Fragments arranged by size:
1712 1443 1292 471 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2095 2127 5454 5486 2095
Size: 32 3327 32 2103
Fragments arranged by size:
3327 2103 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5494
XbaI T'CTAG_A
Cuts at: 2830 2830
Size: 5494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2752 2752
Size: 5494
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5494
XmaI C'CCGG_G
Cuts at: 1870 2819 1870
Size: 949 4545
XmnI GAAnn'nnTTC
Cuts at: 2172 3623 2172
Size: 1451 4043
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3302 276
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI