Restriction map for
pFN21AE0911
(Circular) (Six-base) MAPSORT of: pFN21AE0911.seq Check: 7089 from: 1 to: 5422
pFN21AE0911 5422 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2229 2453 2229
Size: 224 5198
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3232 278
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2765 1937
Size: 828 4594
Acc65I G'GTAC_C
Cuts at: 2743 2743
Size: 5422
AclI AA'CG_TT
Cuts at: 1563 3549 3922 1563
Size: 1986 373 3063
Fragments arranged by size:
3063 1986 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2670 2736 3485 4869 5137 1632
Size: 1038 66 749 1384 268 1917
Fragments arranged by size:
1917 1384 1038 749 268 66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5225
AflIII A'CryG_T
Cuts at: 1203 4321 5298 1203
Size: 3118 977 1327
Fragments arranged by size:
3118 1327 977
AgeI A'CCGG_T
Cuts at: 4253 4253
Size: 5422
AhdI GACnn_n'nnGTC
Cuts at: 4151 4151
Size: 5422
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5422
AlwNI CAG_nnn'CTG
Cuts at: 3165 4737 3165
Size: 1572 3850
ApaI G_GGCC'C
Cuts at: 2170 2170
Size: 5422
ApaLI G'TGCA_C
Cuts at: 3479 4635 3479
Size: 1156 4266
ApoI r'AATT_y
Cuts at: 1493 2675 2683 2731 2957 3109 5218 1493
Size: 1182 8 48 226 152 2109 1697
Fragments arranged by size:
2109 1697 1182 226 152 48 8
AseI AT'TA_AT
Cuts at: 160 1032 3976 160
Size: 872 2944 1606
Fragments arranged by size:
2944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
AvaI C'yCGr_G
Cuts at: 1870 1943 2747 1870
Size: 73 804 4545
Fragments arranged by size:
4545 804 73
BamHI G'GATC_C
Cuts at: 2493 2752 3119 2493
Size: 259 367 4796
Fragments arranged by size:
4796 367 259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2743 4198 618
Size: 325 217 627 956 1455 1842
Fragments arranged by size:
1842 1455 956 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2170 2544 2741 1391
Size: 779 374 197 4072
Fragments arranged by size:
4072 779 374 197
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2724 928
Size: 971 825 3626
Fragments arranged by size:
3626 971 825
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3613 3647 1903
Size: 34 50 34 1592 34 3678
Fragments arranged by size:
3678 1592 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3314 4524 3314
Size: 1210 4212
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2331 4033 136
Size: 107 122 71 631 605 659 1702 1525
Fragments arranged by size:
1702 1525 659 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5417 5417
Size: 5422
BlpI GC'TnA_GC
Cuts at: 2839 2839
Size: 5422
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2170 3483 4639 1790
Size: 277 103 1313 1156 2573
Fragments arranged by size:
2573 1313 1156 277 103
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3153 4111 442
Size: 416 53 83 399 24 1736 958 1753
Fragments arranged by size:
1753 1736 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2072 2156 4082 1239
Size: 818 15 84 1926 2579
Fragments arranged by size:
2579 1926 818 84 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3547 4412 4710 3547
Size: 865 298 4259
Fragments arranged by size:
4259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4085 882
Size: 617 2586 2219
Fragments arranged by size:
2586 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2241 2410 3213 493
Size: 711 1037 169 803 2702
Fragments arranged by size:
2702 1037 803 711 169
BsaBI GATnn'nnATC
Cuts at: 5416 5416
Size: 5422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2205 3229 3611 4246
Size: 53 83 186 936 672 1024 382 635
Cuts at: 4246 275
Size: 1451
Fragments arranged by size:
1451 1024 936 672 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3855 4253 4527 4674 5085 3855
Size: 398 274 147 411 4192
Fragments arranged by size:
4192 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5197
Fragments arranged by size:
5197 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2273 1186
Size: 1087 4335
BseYI C'CCAG_C
Cuts at: 2329 4625 5121 2329
Size: 2296 496 2630
Fragments arranged by size:
2630 2296 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2057 2138 2143 2618 1163
Size: 573 321 81 5 475 3967
Fragments arranged by size:
3967 573 475 321 81 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2872 3633 4661 1540
Size: 275 186 871 761 1028 2301
Fragments arranged by size:
2301 1028 871 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3483 3568 4639 3483
Size: 85 1071 4266
Fragments arranged by size:
4266 1071 85
BsmI GAATG_Cn'
Cuts at: 2933 3026 2933
Size: 93 5329
BsmBI CGTCTCn'nnnn_
Cuts at: 2212 2212
Size: 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2170 2544 2741 3483 3568 4639
Size: 399 277 103 374 197 742 85 1071
Cuts at: 4639 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 374 277 197 103
85
BspEI T'CCGG_A
Cuts at: 5085 5085
Size: 5422
BspHI T'CATG_A
Cuts at: 2022 3311 5041 2022
Size: 1289 1730 2403
Fragments arranged by size:
2403 1730 1289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 2453 2777 844
Size: 1385 224 324 3489
Fragments arranged by size:
3489 1385 324 224
BsrBI CCG'CTC
Cuts at: 1394 1823 3309 1394
Size: 429 1486 3507
Fragments arranged by size:
3507 1486 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2370 3917 4091 65
Size: 1183 1122 1547 174 1396
Fragments arranged by size:
1547 1396 1183 1122 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2201 2325 4066 4253 1700
Size: 110 391 124 1741 187 2869
Fragments arranged by size:
2869 1741 391 187 124 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5422
BssHII G'CGCG_C
Cuts at: 1925 2197 1925
Size: 272 5150
BssSI C'ACGA_G
Cuts at: 2075 3482 4494 2075
Size: 1407 1012 3003
Fragments arranged by size:
3003 1407 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2445 794
Size: 1651 3771
BstBI TT'CG_AA
Cuts at: 4226 4226
Size: 5422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5422
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5422
BstYI r'GATC_y
Cuts at: 1972 2493 2752 3119 3504 3521 4973 5059 5071
Size: 521 259 367 385 17 1452 86 12
Cuts at: 5071 5417 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 521 385 367 346 259 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4870
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4683
Fragments arranged by size:
4683 683 56
BtsI GCAGTG_nn'
Cuts at: 2155 2938 3732 3752 2155
Size: 783 794 20 3825
Fragments arranged by size:
3825 794 783 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5422
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5387
DraI TTT'AAA
Cuts at: 2726 2726
Size: 5422
DraIII CAC_nnn'GTG
Cuts at: 2080 2596 2080
Size: 516 4906
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2306 4429 809
Size: 507 990 2123 1802
Fragments arranged by size:
2123 1802 990 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2179 2869 3758
Size: 54 1425 50 275 18 349 690 889
Cuts at: 3758 5387 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 690 349 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2869 1537
Size: 275 1057 4090
Fragments arranged by size:
4090 1057 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3352 1353
Size: 1999 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4009 4383 4529 387
Size: 1064 138 2420 374 146 1280
Fragments arranged by size:
2420 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2072 2156 2670 2736 3485 4082
Size: 393 425 15 84 514 66 749 597
Cuts at: 4082 4869 5137 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 749 597 514 425 393 268
84 66 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2111 2111
Size: 5422
EcoO109I rG'GnC_Cy
Cuts at: 2166 2167 2166
Size: 1 5421
EcoRI G'AATT_C
Cuts at: 2731 3109 2731
Size: 378 5044
FspI TGC'GCA
Cuts at: 3928 3928
Size: 5422
HaeII r_GCGC'y
Cuts at: 1678 4569 1678
Size: 2891 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2504 2536 4063 4095 4137
Size: 32 135 32 932 32 1527 32 42
Cuts at: 4137 4169 1373
Size: 32 2626
Fragments arranged by size:
2626 1527 932 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2766 3012 669
Size: 988 281 828 246 3079
Fragments arranged by size:
3079 988 828 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5422
HpaI GTT'AAC
Cuts at: 3012 3012
Size: 5422
KpnI G_GTAC'C
Cuts at: 2747 2747
Size: 5422
MfeI C'AATT_G
Cuts at: 3021 3021
Size: 5422
MluI A'CGCG_T
Cuts at: 5298 5298
Size: 5422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4536 4720 1305
Size: 3231 184 2007
Fragments arranged by size:
3231 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3600
Fragments arranged by size:
3600 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3380 3739 3898 518
Size: 648 2214 359 159 2042
Fragments arranged by size:
2214 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2152 2185 2838 3515 4663 4908 1606
Size: 546 33 653 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 653 546 245 33
NaeI GCC'GGC
Cuts at: 2203 2327 2203
Size: 124 5298
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4870
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5422
NgoMIV G'CCGG_C
Cuts at: 2201 2325 2201
Size: 124 5298
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5422
NotI GC'GGCC_GC
Cuts at: 2869 2869
Size: 5422
NspI r_CATG'y
Cuts at: 1132 1229 2780 4325 1132
Size: 97 1551 1545 2229
Fragments arranged by size:
2229 1551 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2011 1348
Size: 663 4759
PciI A'CATG_T
Cuts at: 4321 4321
Size: 5422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5422
PfoI T'CCnGG_A
Cuts at: 2133 2380 2133
Size: 247 5175
PmeI GTTT'AAAC
Cuts at: 2726 2726
Size: 5422
PmlI CAC'GTG
Cuts at: 2241 2410 2241
Size: 169 5253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 5004 5036 3491
Size: 32 1481 32 3877
Fragments arranged by size:
3877 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5422
PsiI TTA'TAA
Cuts at: 2992 2992
Size: 5422
PspOMI G'GGCC_C
Cuts at: 2166 2166
Size: 5422
PstI C_TGCA'G
Cuts at: 830 2774 830
Size: 1944 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5422
PvuII CAG'CTG
Cuts at: 2152 2185 2152
Size: 33 5389
SalI G'TCGA_C
Cuts at: 1936 2764 1936
Size: 828 4594
SbfI CC_TGCA'GG
Cuts at: 2774 2774
Size: 5422
ScaI AGT'ACT
Cuts at: 3670 5382 3670
Size: 1712 3710
SfcI C'TryA_G
Cuts at: 826 1045 2770 3905 4586 4777 826
Size: 219 1725 1135 681 191 1471
Fragments arranged by size:
1725 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
SgrAI Cr'CCGG_yG
Cuts at: 1700 2201 1700
Size: 501 4921
SmaI CCC'GGG
Cuts at: 1872 2749 1872
Size: 877 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3526 4427 4689 820
Size: 197 926 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3213 493
Size: 2720 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5422
SphI G_CATG'C
Cuts at: 1132 2780 1132
Size: 1648 3774
SspI AAT'ATT
Cuts at: 5 52 3346 5
Size: 47 3294 2081
Fragments arranged by size:
3294 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5422
StyI C'CwwG_G
Cuts at: 513 1065 2861 513
Size: 552 1796 3074
Fragments arranged by size:
3074 1796 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3449 3466 3619 3804 4249 325
Size: 1255 1869 17 153 185 445 1498
Fragments arranged by size:
1869 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2055 3668 5380
Size: 274 80 33 51 1443 78 1613 1712
Cuts at: 5380 96
Size: 138
Fragments arranged by size:
1712 1613 1443 274 138 80 78 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5382 5414 5382
Size: 32 5390
Tth111I GACn'n_nGTC
Cuts at: 1316 2129 1316
Size: 813 4609
XbaI T'CTAG_A
Cuts at: 2758 2758
Size: 5422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5422
XmaI C'CCGG_G
Cuts at: 1870 2747 1870
Size: 877 4545
XmnI GAAnn'nnTTC
Cuts at: 2522 3551 2522
Size: 1029 4393
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3230 276
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI