Restriction map for
pFN21AE0910
(Circular) (Six-base) MAPSORT of: pFN21AE0910.seq Check: 319 from: 1 to: 5398
pFN21AE0910 5398 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3208 278
Size: 53 83 186 936 1672 2468
Fragments arranged by size:
2468 1672 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2741 1937
Size: 804 4594
Acc65I G'GTAC_C
Cuts at: 2719 2719
Size: 5398
AclI AA'CG_TT
Cuts at: 1563 3525 3898 1563
Size: 1962 373 3063
Fragments arranged by size:
3063 1962 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 3461 4845 5113 1632
Size: 731 1098 1384 268 1917
Fragments arranged by size:
1917 1384 1098 731 268
AfeI AGC'GCT
Cuts at: 2433 2433
Size: 5398
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5201
AflIII A'CryG_T
Cuts at: 1203 4297 5274 1203
Size: 3094 977 1327
Fragments arranged by size:
3094 1327 977
AgeI A'CCGG_T
Cuts at: 4229 4229
Size: 5398
AhdI GACnn_n'nnGTC
Cuts at: 4127 4127
Size: 5398
AleI CACnn'nnGTG
Cuts at: 1166 2541 1166
Size: 1375 4023
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5398
AlwNI CAG_nnn'CTG
Cuts at: 2123 2638 3141 4713 2123
Size: 515 503 1572 2808
Fragments arranged by size:
2808 1572 515 503
ApaLI G'TGCA_C
Cuts at: 3455 4611 3455
Size: 1156 4242
ApoI r'AATT_y
Cuts at: 1493 2707 2933 3085 5194 1493
Size: 1214 226 152 2109 1697
Fragments arranged by size:
2109 1697 1214 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3952 160
Size: 872 2920 1606
Fragments arranged by size:
2920 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5398
AvaI C'yCGr_G
Cuts at: 1870 1943 2723 1870
Size: 73 780 4545
Fragments arranged by size:
4545 780 73
BamHI G'GATC_C
Cuts at: 2063 2728 3095 2063
Size: 665 367 4366
Fragments arranged by size:
4366 665 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2719 4174 618
Size: 325 217 627 932 1455 1842
Fragments arranged by size:
1842 1455 932 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2664 2717 1391
Size: 1273 53 4072
Fragments arranged by size:
4072 1273 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2483 928
Size: 971 584 3843
Fragments arranged by size:
3843 971 584
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3589 3623 1903
Size: 34 1652 34 3678
Fragments arranged by size:
3678 1652 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3290 4500 3290
Size: 1210 4188
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5398
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4009 136
Size: 107 122 71 631 605 2337 1525
Fragments arranged by size:
2337 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5393 5393
Size: 5398
BlpI GC'TnA_GC
Cuts at: 2815 2815
Size: 5398
Bme1580I G_kGCm'C
Cuts at: 1790 3459 4615 1790
Size: 1669 1156 2573
Fragments arranged by size:
2573 1669 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5398
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3129 4087 442
Size: 416 53 83 399 24 1712 958 1753
Fragments arranged by size:
1753 1712 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2253 1056
Size: 1197 4201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 2504 4058 1239
Size: 1163 102 1554 2579
Fragments arranged by size:
2579 1554 1163 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3523 4388 4686 3523
Size: 865 298 4235
Fragments arranged by size:
4235 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2247 4061 882
Size: 617 748 1814 2219
Fragments arranged by size:
2219 1814 748 617
BsaAI yAC'GTr
Cuts at: 493 1204 3189 493
Size: 711 1985 2702
Fragments arranged by size:
2702 1985 711
BsaBI GATnn'nnATC
Cuts at: 5392 5392
Size: 5398
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3205 3587 4222 275
Size: 53 83 186 936 1672 382 635 1451
Fragments arranged by size:
1672 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2408 3831 4229 4503 4650 5061 2408
Size: 1423 398 274 147 411 2745
Fragments arranged by size:
2745 1423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2418 2448 1178
Size: 30 165 30 1015 30 4128
Fragments arranged by size:
4128 1015 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5398
BseYI C'CCAG_C
Cuts at: 4601 5097 4601
Size: 496 4902
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2092 2656 1163
Size: 573 356 564 3905
Fragments arranged by size:
3905 573 564 356
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2463 2848 3609 4637 1540
Size: 275 186 462 385 761 1028 2301
Fragments arranged by size:
2301 1028 761 462 385 275 186
BsiHKAI G_wGCw'C
Cuts at: 3459 3544 4615 3459
Size: 85 1071 4242
Fragments arranged by size:
4242 1071 85
BsmI GAATG_Cn'
Cuts at: 2909 3002 2909
Size: 93 5305
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2664 2717 3459 3544 4615 1391
Size: 399 874 53 742 85 1071 2174
Fragments arranged by size:
2174 1071 874 742 399 85 53
BspEI T'CCGG_A
Cuts at: 5061 5061
Size: 5398
BspHI T'CATG_A
Cuts at: 3287 5017 3287
Size: 1730 3668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2000 2753 844
Size: 1156 753 3489
Fragments arranged by size:
3489 1156 753
BsrBI CCG'CTC
Cuts at: 1394 1823 3285 1394
Size: 429 1462 3507
Fragments arranged by size:
3507 1462 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3893 4067 65
Size: 1183 2645 174 1396
Fragments arranged by size:
2645 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4042 4229 1700
Size: 110 2232 187 2869
Fragments arranged by size:
2869 2232 187 110
BsrGI T'GTAC_A
Cuts at: 96 2469 96
Size: 2373 3025
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5398
BssSI C'ACGA_G
Cuts at: 3458 4470 3458
Size: 1012 4386
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5398
BstBI TT'CG_AA
Cuts at: 4202 4202
Size: 5398
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5398
BstYI r'GATC_y
Cuts at: 1972 2063 2728 3095 3480 3497 4949 5035 5047
Size: 91 665 367 385 17 1452 86 12
Cuts at: 5047 5393 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 665 385 367 346 91 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2193 2193
Size: 5398
BtgI C'CryG_G
Cuts at: 513 1065 2004 2448 513
Size: 552 939 444 3463
Fragments arranged by size:
3463 939 552 444
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4659
Fragments arranged by size:
4659 683 56
BtsI GCAGTG_nn'
Cuts at: 2914 3708 3728 2914
Size: 794 20 4584
Fragments arranged by size:
4584 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5398
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5363
DraI TTT'AAA
Cuts at: 2551 2702 2551
Size: 151 5247
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4405 809
Size: 507 3089 1802
Fragments arranged by size:
3089 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2460 2513 2845
Size: 54 1425 50 275 18 630 53 332
Cuts at: 2845 3734 5363 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 630 332 275 54 53
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2460 2845 1537
Size: 275 648 385 4090
Fragments arranged by size:
4090 648 385 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2652 3328 1353
Size: 1299 676 3423
Fragments arranged by size:
3423 1299 676
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3985 4359 4505 387
Size: 1064 138 2396 374 146 1280
Fragments arranged by size:
2396 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2402 2504 3461 4058 4845 5113
Size: 393 731 39 102 957 597 787 268
Cuts at: 5113 1239
Size: 1524
Fragments arranged by size:
1524 957 787 731 597 393 268 102
39
EcoRI G'AATT_C
Cuts at: 2707 3085 2707
Size: 378 5020
EcoRV GAT'ATC
Cuts at: 2170 2170
Size: 5398
FspI TGC'GCA
Cuts at: 3904 3904
Size: 5398
HaeII r_GCGC'y
Cuts at: 1678 2435 4545 1678
Size: 757 2110 2531
Fragments arranged by size:
2531 2110 757
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2538 2570 4039 4071 4113
Size: 32 135 32 966 32 1469 32 42
Cuts at: 4113 4145 1373
Size: 32 2626
Fragments arranged by size:
2626 1469 966 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2742 2988 669
Size: 988 281 804 246 3079
Fragments arranged by size:
3079 988 804 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5398
HpaI GTT'AAC
Cuts at: 2988 2988
Size: 5398
KpnI G_GTAC'C
Cuts at: 2723 2723
Size: 5398
MfeI C'AATT_G
Cuts at: 2997 2997
Size: 5398
MluI A'CGCG_T
Cuts at: 5274 5274
Size: 5398
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4512 4696 1305
Size: 3207 184 2007
Fragments arranged by size:
3207 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2515 10
Size: 54 1425 343 683 2893
Fragments arranged by size:
2893 1425 683 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2541 3356 3715 3874 518
Size: 648 1375 815 359 159 2042
Fragments arranged by size:
2042 1375 815 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2314 2814 3491 4639 4884 1606
Size: 708 500 677 1148 245 2120
Fragments arranged by size:
2120 1148 708 677 500 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3907
Fragments arranged by size:
3907 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5398
NheI G'CTAG_C
Cuts at: 1052 2249 1052
Size: 1197 4201
NotI GC'GGCC_GC
Cuts at: 2845 2845
Size: 5398
NspI r_CATG'y
Cuts at: 1132 1229 2756 4301 1132
Size: 97 1527 1545 2229
Fragments arranged by size:
2229 1545 1527 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5398
PciI A'CATG_T
Cuts at: 4297 4297
Size: 5398
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5398
PmeI GTTT'AAAC
Cuts at: 2702 2702
Size: 5398
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3467 3499 4980 5012 3467
Size: 32 1481 32 3853
Fragments arranged by size:
3853 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2483 1613
Size: 870 4528
PsiI TTA'TAA
Cuts at: 2968 2968
Size: 5398
PstI C_TGCA'G
Cuts at: 830 2643 2750 830
Size: 1813 107 3478
Fragments arranged by size:
3478 1813 107
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5398
SalI G'TCGA_C
Cuts at: 1936 2740 1936
Size: 804 4594
SapI GCTCTTCn'nnn_
Cuts at: 2652 2652
Size: 5398
SbfI CC_TGCA'GG
Cuts at: 2750 2750
Size: 5398
ScaI AGT'ACT
Cuts at: 2022 3646 5358 2022
Size: 1624 1712 2062
Fragments arranged by size:
2062 1712 1624
SfcI C'TryA_G
Cuts at: 826 1045 2082 2639 2746 3881 4562 4753 826
Size: 219 1037 557 107 1135 681 191 1471
Fragments arranged by size:
1471 1135 1037 681 557 219 191 107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5398
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5398
SmaI CCC'GGG
Cuts at: 1872 2725 1872
Size: 853 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3502 4403 4665 820
Size: 197 926 1559 901 262 1553
Fragments arranged by size:
1559 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3189 493
Size: 2696 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5398
SphI G_CATG'C
Cuts at: 1132 2756 1132
Size: 1624 3774
SspI AAT'ATT
Cuts at: 5 52 3322 5
Size: 47 3270 2081
Fragments arranged by size:
3270 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5398
StyI C'CwwG_G
Cuts at: 513 1065 2004 2837 513
Size: 552 939 833 3074
Fragments arranged by size:
3074 939 833 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3425 3442 3595 3780 4225 325
Size: 1255 1845 17 153 185 445 1498
Fragments arranged by size:
1845 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2020 2316 2469
Size: 274 80 33 51 1443 43 296 153
Cuts at: 2469 3644 5356 96
Size: 1175 1712 138
Fragments arranged by size:
1712 1443 1175 296 274 153 138 80
51 43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5358 5390 5358
Size: 32 5366
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5398
XbaI T'CTAG_A
Cuts at: 2734 2734
Size: 5398
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5398
XmaI C'CCGG_G
Cuts at: 1870 2723 1870
Size: 853 4545
XmnI GAAnn'nnTTC
Cuts at: 3527 3527
Size: 5398
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3206 276
Size: 53 83 186 936 1672 2468
Fragments arranged by size:
2468 1672 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI