Restriction map for
pFN21AE0908
(Circular) (Six-base) MAPSORT of: pFN21AE0908.seq Check: 2815 from: 1 to: 5380
pFN21AE0908 5380 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3190 278
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2723 1937
Size: 786 4594
Acc65I G'GTAC_C
Cuts at: 2701 2701
Size: 5380
AclI AA'CG_TT
Cuts at: 1563 3507 3880 1563
Size: 1944 373 3063
Fragments arranged by size:
3063 1944 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2588 3443 4827 5095 1632
Size: 956 855 1384 268 1917
Fragments arranged by size:
1917 1384 956 855 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5183
AflIII A'CryG_T
Cuts at: 1203 4279 5256 1203
Size: 3076 977 1327
Fragments arranged by size:
3076 1327 977
AgeI A'CCGG_T
Cuts at: 4211 4211
Size: 5380
AhdI GACnn_n'nnGTC
Cuts at: 4109 4109
Size: 5380
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2088 1184
Size: 904 4476
AlwNI CAG_nnn'CTG
Cuts at: 2111 2431 2488 2645 3123 4695 2111
Size: 320 57 157 478 1572 2796
Fragments arranged by size:
2796 1572 478 320 157 57
ApaI G_GGCC'C
Cuts at: 2204 2204
Size: 5380
ApaLI G'TGCA_C
Cuts at: 2050 3437 4593 2050
Size: 1387 1156 2837
Fragments arranged by size:
2837 1387 1156
ApoI r'AATT_y
Cuts at: 1493 2689 2915 3067 5176 1493
Size: 1196 226 152 2109 1697
Fragments arranged by size:
2109 1697 1196 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3934 160
Size: 872 2902 1606
Fragments arranged by size:
2902 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
AvaI C'yCGr_G
Cuts at: 1870 1943 2705 1870
Size: 73 762 4545
Fragments arranged by size:
4545 762 73
BamHI G'GATC_C
Cuts at: 2710 3077 2710
Size: 367 5013
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2064 2701 4156 618
Size: 325 217 627 277 637 1455 1842
Fragments arranged by size:
1842 1455 637 627 325 277 217
BanII G_rGCy'C
Cuts at: 1391 2204 2671 2699 1391
Size: 813 467 28 4072
Fragments arranged by size:
4072 813 467 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2007 2041 3571 3605 1903
Size: 34 70 34 1530 34 3678
Fragments arranged by size:
3678 1530 70 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3272 4482 3272
Size: 1210 4170
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5380
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2427 2436 3991
Size: 107 122 71 631 605 755 9 1555
Cuts at: 3991 136
Size: 1525
Fragments arranged by size:
1555 1525 755 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 5375 5375
Size: 5380
BlpI GC'TnA_GC
Cuts at: 2385 2797 2385
Size: 412 4968
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2204 3441 4597 1790
Size: 264 150 1237 1156 2573
Fragments arranged by size:
2573 1237 1156 264 150
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5380
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3111 4069 442
Size: 416 53 83 399 24 1694 958 1753
Fragments arranged by size:
1753 1694 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2408 2525 2622 4040 1239
Size: 935 234 117 97 1418 2579
Fragments arranged by size:
2579 1418 935 234 117 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2603 3505 4370 4668 2603
Size: 902 865 298 3315
Fragments arranged by size:
3315 902 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4043 882
Size: 617 2544 2219
Fragments arranged by size:
2544 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3171 493
Size: 711 1967 2702
Fragments arranged by size:
2702 1967 711
BsaBI GATnn'nnATC
Cuts at: 5374 5374
Size: 5380
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2451 3187 3569 4204
Size: 53 83 186 936 918 736 382 635
Cuts at: 4204 275
Size: 1451
Fragments arranged by size:
1451 936 918 736 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3813 4211 4485 4632 5043 3813
Size: 398 274 147 411 4150
Fragments arranged by size:
4150 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2497 2527 1178
Size: 30 165 30 1094 30 4031
Fragments arranged by size:
4031 1094 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5380
BseYI C'CCAG_C
Cuts at: 2177 2434 2481 4583 5079 2177
Size: 257 47 2102 496 2478
Fragments arranged by size:
2478 2102 496 257 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2398 2642 1163
Size: 573 662 244 3901
Fragments arranged by size:
3901 662 573 244
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2830 3591 4619 1540
Size: 275 186 829 761 1028 2301
Fragments arranged by size:
2301 1028 829 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2054 2061 2160 3441 3526 4597 2054
Size: 7 99 1281 85 1071 2837
Fragments arranged by size:
2837 1281 1071 99 85 7
BsmI GAATG_Cn'
Cuts at: 2891 2984 2891
Size: 93 5287
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2061 2160 2204 2671 2699 3441
Size: 399 264 7 99 44 467 28 742
Cuts at: 3441 3526 4597 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 467 399 264 99 85
44 28 7
BspEI T'CCGG_A
Cuts at: 5043 5043
Size: 5380
BspHI T'CATG_A
Cuts at: 3269 4999 3269
Size: 1730 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2735 844
Size: 1891 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3267 1394
Size: 429 1444 3507
Fragments arranged by size:
3507 1444 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2442 3875 4049 65
Size: 1183 1194 1433 174 1396
Fragments arranged by size:
1433 1396 1194 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2613 4024 4211 1700
Size: 110 803 1411 187 2869
Fragments arranged by size:
2869 1411 803 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5380
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5380
BssSI C'ACGA_G
Cuts at: 2026 3440 4452 2026
Size: 1414 1012 2954
Fragments arranged by size:
2954 1414 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5380
BstBI TT'CG_AA
Cuts at: 4184 4184
Size: 5380
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5380
BstYI r'GATC_y
Cuts at: 1972 2710 3077 3462 3479 4931 5017 5029 5375
Size: 738 367 385 17 1452 86 12 346
Cuts at: 5375 1972
Size: 1977
Fragments arranged by size:
1977 1452 738 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3889
Fragments arranged by size:
3889 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4641
Fragments arranged by size:
4641 683 56
BtsI GCAGTG_nn'
Cuts at: 2082 2379 2474 2896 3690 3710 2082
Size: 297 95 422 794 20 3752
Fragments arranged by size:
3752 794 422 297 95 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5345
DraI TTT'AAA
Cuts at: 2684 2684
Size: 5380
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4387 809
Size: 507 3071 1802
Fragments arranged by size:
3071 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2598 2827 3716
Size: 54 1425 50 275 18 768 229 889
Cuts at: 3716 5345 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 768 275 229 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2827 1537
Size: 275 1015 4090
Fragments arranged by size:
4090 1015 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3310 1353
Size: 1957 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3967 4341 4487 387
Size: 1064 138 2378 374 146 1280
Fragments arranged by size:
2378 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2408 2525 2588 2622 3443 4040
Size: 393 542 234 117 63 34 821 597
Cuts at: 4040 4827 5095 1239
Size: 787 268 1524
Fragments arranged by size:
1524 821 787 597 542 393 268 234
117 63 34
EcoO109I rG'GnC_Cy
Cuts at: 2101 2108 2181 2201 2428 2101
Size: 7 73 20 227 5053
Fragments arranged by size:
5053 227 73 20 7
EcoRI G'AATT_C
Cuts at: 2689 3067 2689
Size: 378 5002
FspI TGC'GCA
Cuts at: 3886 3886
Size: 5380
HaeII r_GCGC'y
Cuts at: 1678 2596 4527 1678
Size: 918 1931 2531
Fragments arranged by size:
2531 1931 918
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2620 2652 4021 4053 4095
Size: 32 135 32 1048 32 1369 32 42
Cuts at: 4095 4127 1373
Size: 32 2626
Fragments arranged by size:
2626 1369 1048 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2272 2724 2970 669
Size: 988 281 334 452 246 3079
Fragments arranged by size:
3079 988 452 334 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5380
HpaI GTT'AAC
Cuts at: 2970 2970
Size: 5380
KpnI G_GTAC'C
Cuts at: 2705 2705
Size: 5380
MfeI C'AATT_G
Cuts at: 2979 2979
Size: 5380
MluI A'CGCG_T
Cuts at: 5256 5256
Size: 5380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4494 4678 1305
Size: 3189 184 2007
Fragments arranged by size:
3189 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3558
Fragments arranged by size:
3558 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3338 3697 3856 518
Size: 648 2172 359 159 2042
Fragments arranged by size:
2172 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2488 2645 2796 3473 4621 4866 1606
Size: 882 157 151 677 1148 245 2120
Fragments arranged by size:
2120 1148 882 677 245 157 151
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3889
Fragments arranged by size:
3889 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5380
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5380
NotI GC'GGCC_GC
Cuts at: 2827 2827
Size: 5380
NspI r_CATG'y
Cuts at: 1132 1229 2738 4283 1132
Size: 97 1509 1545 2229
Fragments arranged by size:
2229 1545 1509 97
PasI CC'CwG_GG
Cuts at: 1348 2105 2259 1348
Size: 757 154 4469
Fragments arranged by size:
4469 757 154
PciI A'CATG_T
Cuts at: 4279 4279
Size: 5380
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2111 2548 1108
Size: 1003 437 3940
Fragments arranged by size:
3940 1003 437
PmeI GTTT'AAAC
Cuts at: 2684 2684
Size: 5380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 4962 4994 3449
Size: 32 1481 32 3835
Fragments arranged by size:
3835 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2101 2181 2101
Size: 80 5300
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5380
PsiI TTA'TAA
Cuts at: 2950 2950
Size: 5380
PspOMI G'GGCC_C
Cuts at: 2200 2200
Size: 5380
PstI C_TGCA'G
Cuts at: 830 2077 2580 2650 2732 830
Size: 1247 503 70 82 3478
Fragments arranged by size:
3478 1247 503 82 70
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5380
PvuII CAG'CTG
Cuts at: 2645 2645
Size: 5380
SalI G'TCGA_C
Cuts at: 1936 2722 1936
Size: 786 4594
SanDI GG'GwC_CC
Cuts at: 2101 2101
Size: 5380
SbfI CC_TGCA'GG
Cuts at: 2732 2732
Size: 5380
ScaI AGT'ACT
Cuts at: 3628 5340 3628
Size: 1712 3668
SfcI C'TryA_G
Cuts at: 826 1045 2073 2332 2576 2646 2728 3863 4544
Size: 219 1028 259 244 70 82 1135 681
Cuts at: 4544 4735 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1028 681 259 244 219 191
82 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5380
SmaI CCC'GGG
Cuts at: 1872 2707 1872
Size: 835 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2618 3484 4385 4647 820
Size: 197 926 675 866 901 262 1553
Fragments arranged by size:
1553 926 901 866 675 262 197
SnaBI TAC'GTA
Cuts at: 493 3171 493
Size: 2678 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5380
SphI G_CATG'C
Cuts at: 1132 2738 1132
Size: 1606 3774
SspI AAT'ATT
Cuts at: 5 52 3304 5
Size: 47 3252 2081
Fragments arranged by size:
3252 2081 47
StuI AGG'CCT
Cuts at: 1517 2330 1517
Size: 813 4567
StyI C'CwwG_G
Cuts at: 513 1065 2004 2601 2819 513
Size: 552 939 597 218 3074
Fragments arranged by size:
3074 939 597 552 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3407 3424 3577 3762 4207 325
Size: 1255 1827 17 153 185 445 1498
Fragments arranged by size:
1827 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3626 5338 96
Size: 274 80 33 51 1443 1649 1712 138
Fragments arranged by size:
1712 1649 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2497 2529 5340 5372 2497
Size: 32 2811 32 2505
Fragments arranged by size:
2811 2505 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5380
XbaI T'CTAG_A
Cuts at: 2716 2716
Size: 5380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2272 2489 2272
Size: 217 5163
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5380
XmaI C'CCGG_G
Cuts at: 1870 2705 1870
Size: 835 4545
XmnI GAAnn'nnTTC
Cuts at: 3509 3509
Size: 5380
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3188 276
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI