Restriction map for
pFN21AE0900
(Circular) (Six-base) MAPSORT of: pFN21AE0900.seq Check: 2045 from: 1 to: 14959
pFN21AE0900 14959 bp
With 182 enzymes: SgfI *
December 21, 2016 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7537 7537
Size: 14959
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 12769 278
Size: 53 83 186 936 11233 2468
Fragments arranged by size:
11233 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2341 5532 6868 9626 10370 11830 12302 1937
Size: 404 3191 1336 2758 744 1460 472 4594
Fragments arranged by size:
4594 3191 2758 1460 1336 744 472 404
Acc65I G'GTAC_C
Cuts at: 12280 12280
Size: 14959
AclI AA'CG_TT
Cuts at: 1563 4494 13086 13459 1563
Size: 2931 8592 373 3063
Fragments arranged by size:
8592 3063 2931 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2097 2744 2934 3093 3312 3624 3981 4662
Size: 465 647 190 159 219 312 357 681
Cuts at: 4662 5014 5718 7851 8415 9440 9717 11158 11586
Size: 352 704 2133 564 1025 277 1441 428
Cuts at: 11586 13022 14406 14674 1632
Size: 1436 1384 268 1917
Fragments arranged by size:
2133 1917 1441 1436 1384 1025 704 681
647 564 465 428 357 352 312 277
268 219 190 159
AflII C'TTAA_G
Cuts at: 820 1017 6850 820
Size: 197 5833 8929
Fragments arranged by size:
8929 5833 197
AflIII A'CryG_T
Cuts at: 1203 2452 4028 8768 9508 13858 14835 1203
Size: 1249 1576 4740 740 4350 977 1327
Fragments arranged by size:
4740 4350 1576 1327 1249 977 740
AgeI A'CCGG_T
Cuts at: 11119 13790 11119
Size: 2671 12288
AhdI GACnn_n'nnGTC
Cuts at: 4905 10245 13688 4905
Size: 5340 3443 6176
Fragments arranged by size:
6176 5340 3443
AleI CACnn'nnGTG
Cuts at: 1166 10907 1166
Size: 9741 5218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 10707 10722 1184
Size: 9523 15 5421
Fragments arranged by size:
9523 5421 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2313 2345 9579 9611 2313
Size: 32 7234 32 7661
Fragments arranged by size:
7661 7234 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4121 4640 9404 12702 14274 4121
Size: 519 4764 3298 1572 4806
Fragments arranged by size:
4806 4764 3298 1572 519
ApaLI G'TGCA_C
Cuts at: 10668 13016 14172 10668
Size: 2348 1156 11455
Fragments arranged by size:
11455 2348 1156
ApoI r'AATT_y
Cuts at: 1493 2043 2303 2410 2818 2997 3334 3670 3712
Size: 550 260 107 408 179 337 336 42
Cuts at: 3712 5381 5992 6008 6346 6391 6790 7000 7653
Size: 1669 611 16 338 45 399 210 653
Cuts at: 7653 7788 8155 8249 8390 8800 8879 9046 9068
Size: 135 367 94 141 410 79 167 22
Cuts at: 9068 9800 9855 9926 10608 10619 11025 11133 11180
Size: 732 55 71 682 11 406 108 47
Cuts at: 11180 11956 11984 11994 12268 12494 12646 14755 1493
Size: 776 28 10 274 226 152 2109 1697
Fragments arranged by size:
2109 1697 1669 776 732 682 653 611
550 410 408 406 399 367 338 337
336 274 260 226 210 179 167 152
141 135 108 107 94 79 71 55
47 45 42 28 22 16 11 10
AseI AT'TA_AT
Cuts at: 160 1032 5199 9351 13513 160
Size: 872 4167 4152 4162 1606
Fragments arranged by size:
4167 4162 4152 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14959
AvaI C'yCGr_G
Cuts at: 1870 1943 12284 1870
Size: 73 10341 4545
Fragments arranged by size:
10341 4545 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 8985 9018 8985
Size: 33 14926
BamHI G'GATC_C
Cuts at: 2015 2569 9835 12289 12656 2015
Size: 554 7266 2454 367 4318
Fragments arranged by size:
7266 4318 2454 554 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6835 11918 12280 13735 618
Size: 325 217 627 5048 5083 362 1455 1842
Fragments arranged by size:
5083 5048 1842 1455 627 362 325 217
BanII G_rGCy'C
Cuts at: 1391 3223 8832 9814 10286 11166 12278 1391
Size: 1832 5609 982 472 880 1112 4072
Fragments arranged by size:
5609 4072 1832 1112 982 880 472
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2345 4669 8270 9446 10508 11649 928
Size: 971 446 2324 3601 1176 1062 1141 4238
Fragments arranged by size:
4238 3601 2324 1176 1141 1062 971 446
BbvCI CC'TCA_GC
Cuts at: 2562 2562
Size: 14959
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 12017 12051 13150 13184 1903
Size: 34 50 34 9996 34 1099 34 3678
Fragments arranged by size:
9996 3678 1099 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 12851 14061 12851
Size: 1210 13749
BclI T'GATC_A
Cuts at: 1548 4288 6082 6355 12174 1548
Size: 2740 1794 273 5819 4333
Fragments arranged by size:
5819 4333 2740 1794 273
BfrBI ATG'CAT
Cuts at: 7400 8971 11548 7400
Size: 1571 2577 10811
Fragments arranged by size:
10811 2577 1571
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 13570 136
Size: 107 122 71 631 605 11898 1525
Fragments arranged by size:
11898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 14954 14954
Size: 14959
BlpI GC'TnA_GC
Cuts at: 12376 12376
Size: 14959
Bme1580I G_kGCm'C
Cuts at: 1790 10672 13020 14176 1790
Size: 8882 2348 1156 2573
Fragments arranged by size:
8882 2573 2348 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 14959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3853 4217 6485
Size: 416 53 83 399 24 2436 364 2268
Cuts at: 6485 10189 12690 13648 442
Size: 3704 2501 958 1753
Fragments arranged by size:
3704 2501 2436 2268 1753 958 416 399
364 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 6588 1056
Size: 5532 9427
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5205 5205
Size: 14959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3465 8094 8919 9810 11363 11445 13619 1239
Size: 2226 4629 825 891 1553 82 2174 2579
Fragments arranged by size:
4629 2579 2226 2174 1553 891 825 82
Bpu10I CC'TnA_GC
Cuts at: 2562 6843 7009 9944 2562
Size: 4281 166 2935 7577
Fragments arranged by size:
7577 4281 2935 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6174 10775 11845 13084 13949 14247 6174
Size: 4601 1070 1239 865 298 6886
Fragments arranged by size:
6886 4601 1239 1070 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3195 6740 13622 882
Size: 617 1696 3545 6882 2219
Fragments arranged by size:
6882 3545 2219 1696 617
BsaAI yAC'GTr
Cuts at: 493 1204 3347 8105 9630 10667 12750 493
Size: 711 2143 4758 1525 1037 2083 2702
Fragments arranged by size:
4758 2702 2143 2083 1525 1037 711
BsaBI GATnn'nnATC
Cuts at: 3093 5453 5490 14953 3093
Size: 2360 37 9463 3099
Fragments arranged by size:
9463 3099 2360 37
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 10975 12766 13148 13783
Size: 53 83 186 936 9442 1791 382 635
Cuts at: 13783 275
Size: 1451
Fragments arranged by size:
9442 1791 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 11119 13392 13790 14064 14211 14622 11119
Size: 2273 398 274 147 411 11456
Fragments arranged by size:
11456 2273 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2550 2580 3137 3167 9579
Size: 30 165 30 1147 30 557 30 6412
Cuts at: 9579 9609 10431 10461 1178
Size: 30 822 30 5676
Fragments arranged by size:
6412 5676 1147 822 557 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4877 8838 11952 1186
Size: 3691 3961 3114 4193
Fragments arranged by size:
4193 3961 3691 3114
BseYI C'CCAG_C
Cuts at: 10744 14162 14658 10744
Size: 3418 496 11045
Fragments arranged by size:
11045 3418 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5196 10155 10728 11868 1163
Size: 573 3460 4959 573 1140 4254
Fragments arranged by size:
4959 4254 3460 1140 573 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 12409 13170 14198 1540
Size: 275 186 10408 761 1028 2301
Fragments arranged by size:
10408 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2631 8352 10672 13020 13105 14176 2631
Size: 5721 2320 2348 85 1071 3414
Fragments arranged by size:
5721 3414 2348 2320 1071 85
BsmI GAATG_Cn'
Cuts at: 3444 3736 3922 5483 5515 7750 9466 10738 12470
Size: 292 186 1561 32 2235 1716 1272 1732
Cuts at: 12470 12563 3444
Size: 93 5840
Fragments arranged by size:
5840 2235 1732 1716 1561 1272 292 186
93 32
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2631 3223 8352 8832 9814 10286 10672
Size: 399 841 592 5129 480 982 472 386
Cuts at: 10672 11166 12278 13020 13105 14176 1391
Size: 494 1112 742 85 1071 2174
Fragments arranged by size:
5129 2174 1112 1071 982 841 742 592
494 480 472 399 386 85
BspEI T'CCGG_A
Cuts at: 14622 14622
Size: 14959
BspHI T'CATG_A
Cuts at: 2671 2710 4291 4353 4732 5800 12848 14578 2671
Size: 39 1581 62 379 1068 7048 1730 3052
Fragments arranged by size:
7048 3052 1730 1581 1068 379 62 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2149 3725 7537 7667 8387 12314 844
Size: 1305 1576 3812 130 720 3927 3489
Fragments arranged by size:
3927 3812 3489 1576 1305 720 130
BsrBI CCG'CTC
Cuts at: 1394 1823 12846 1394
Size: 429 11023 3507
Fragments arranged by size:
11023 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 9646 10537 11530 13454 13628 65
Size: 1183 8398 891 993 1924 174 1396
Fragments arranged by size:
8398 1924 1396 1183 993 891 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 11119 13603 13790 1700
Size: 110 9309 2484 187 2869
Fragments arranged by size:
9309 2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 2447 10298 11864 96
Size: 2351 7851 1566 3191
Fragments arranged by size:
7851 3191 2351 1566
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 14959
BssSI C'ACGA_G
Cuts at: 8325 13019 14031 8325
Size: 4694 1012 9253
Fragments arranged by size:
9253 4694 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 11554 794
Size: 10760 4199
BstBI TT'CG_AA
Cuts at: 13763 13763
Size: 14959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 14959
BstYI r'GATC_y
Cuts at: 1972 2015 2569 4057 9835 10201 10833 11188 12289
Size: 43 554 1488 5778 366 632 355 1101
Cuts at: 12289 12656 13041 13058 14510 14596 14608 14954 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
5778 1977 1488 1452 1101 632 554 385
367 366 355 346 86 43 17 12
BstZ17I GTA'TAC
Cuts at: 9627 10371 9627
Size: 744 14215
Bsu36I CC'TnA_GG
Cuts at: 9921 9921
Size: 14959
BtgI C'CryG_G
Cuts at: 513 1065 4446 4782 10857 11156 513
Size: 552 3381 336 6075 299 4316
Fragments arranged by size:
6075 4316 3381 552 336 299
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 10407 487
Size: 683 56 9181 5039
Fragments arranged by size:
9181 5039 683 56
BtsI GCAGTG_nn'
Cuts at: 6025 10731 11613 12475 13269 13289 6025
Size: 4706 882 862 794 20 7695
Fragments arranged by size:
7695 4706 882 862 794 20
ClaI AT'CG_AT
Cuts at: 1554 5519 1554
Size: 3965 10994
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 10548 10583 529
Size: 35 9984 35 4905
Fragments arranged by size:
9984 4905 35 35
DraI TTT'AAA
Cuts at: 2191 2827 3592 4476 4917 8053 8395 9235 11980
Size: 636 765 884 441 3136 342 840 2745
Cuts at: 11980 12263 2191
Size: 283 4887
Fragments arranged by size:
4887 3136 2745 884 840 765 636 441
342 283
DraIII CAC_nnn'GTG
Cuts at: 7847 9420 7847
Size: 1573 13386
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2085 2349 4995 11755 13966 809
Size: 507 769 264 2646 6760 2211 1802
Fragments arranged by size:
6760 2646 2211 1802 769 507 264
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3692 6112 10152
Size: 54 1425 50 275 18 1862 2420 4040
Cuts at: 10152 11706 12406 13295 14924 8
Size: 1554 700 889 1629 43
Fragments arranged by size:
4040 2420 1862 1629 1554 1425 889 700
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 12406 1537
Size: 275 10594 4090
Fragments arranged by size:
10594 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3408 4191 5075 5414 7459 8815 10767 11803
Size: 2055 783 884 339 2045 1356 1952 1036
Cuts at: 11803 12889 1353
Size: 1086 3423
Fragments arranged by size:
3423 2055 2045 1952 1356 1086 1036 884
783 339
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 13546 13920 14066 387
Size: 1064 138 11957 374 146 1280
Fragments arranged by size:
11957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2744 2934 3093 3312 3465 3624
Size: 393 465 647 190 159 219 153 159
Cuts at: 3624 3981 4662 5014 5718 7851 8094 8415 8919
Size: 357 681 352 704 2133 243 321 504
Cuts at: 8919 9440 9717 9810 11158 11363 11445 11586 13022
Size: 521 277 93 1348 205 82 141 1436
Cuts at: 13022 13619 14406 14674 1239
Size: 597 787 268 1524
Fragments arranged by size:
2133 1524 1436 1348 787 704 681 647
597 521 504 465 393 357 352 321
277 268 243 219 205 190 159 159
153 141 93 82
EcoNI CCTnn'n_nnAGG
Cuts at: 11333 11634 11333
Size: 301 14658
EcoRI G'AATT_C
Cuts at: 2818 3334 3712 6790 11956 12268 12646 2818
Size: 516 378 3078 5166 312 378 5131
Fragments arranged by size:
5166 5131 3078 516 378 378 312
EcoRV GAT'ATC
Cuts at: 7289 7289
Size: 14959
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3338 3905 5802 8050 8537 3338
Size: 567 1897 2248 487 9760
Fragments arranged by size:
9760 2248 1897 567 487
FspI TGC'GCA
Cuts at: 13465 13465
Size: 14959
HaeII r_GCGC'y
Cuts at: 1678 14106 1678
Size: 12428 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3137 3169 3782 3814 3818
Size: 32 135 32 1565 32 613 32 4
Cuts at: 3818 3850 4115 4147 4250 4282 4325 4357 4891
Size: 32 265 32 103 32 43 32 534
Cuts at: 4891 4904 4923 4936 5818 5850 7631 7663 9025
Size: 13 19 13 882 32 1781 32 1362
Cuts at: 9025 9057 10231 10263 10490 10522 11644 11665 11676
Size: 32 1174 32 227 32 1122 21 11
Cuts at: 11676 11697 13600 13632 13674 13706 1373
Size: 21 1903 32 42 32 2626
Fragments arranged by size:
2626 1903 1781 1565 1362 1174 1122 882
613 534 265 227 135 103 43 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 21
21 19 13 13 11 4
HincII GTy'rAC
Cuts at: 669 1657 1938 6231 7106 11004 12303 12549 669
Size: 988 281 4293 875 3898 1299 246 3079
Fragments arranged by size:
4293 3898 3079 1299 988 875 281 246
HindIII A'AGCT_T
Cuts at: 748 7895 8700 9842 11540 11645 748
Size: 7147 805 1142 1698 105 4062
Fragments arranged by size:
7147 4062 1698 1142 805 105
HpaI GTT'AAC
Cuts at: 6231 11004 12549 6231
Size: 4773 1545 8641
Fragments arranged by size:
8641 4773 1545
KpnI G_GTAC'C
Cuts at: 12284 12284
Size: 14959
MfeI C'AATT_G
Cuts at: 12558 12558
Size: 14959
MluI A'CGCG_T
Cuts at: 9508 14835 9508
Size: 5327 9632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 10821 14073 14257 1305
Size: 9516 3252 184 2007
Fragments arranged by size:
9516 3252 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3694 6114 10154 11708 10
Size: 54 1425 343 1862 2420 4040 1554 3261
Fragments arranged by size:
4040 3261 2420 1862 1554 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4296 6057 6927 7263 9090 10907 11562
Size: 648 3130 1761 870 336 1827 1817 655
Cuts at: 11562 12917 13276 13435 518
Size: 1355 359 159 2042
Fragments arranged by size:
3130 2042 1827 1817 1761 1355 870 655
648 359 336 159
MspA1I CmG'CkG
Cuts at: 1606 2224 2272 6764 9404 9622 10049 12375 13052
Size: 618 48 4492 2640 218 427 2326 677
Cuts at: 13052 14200 14445 1606
Size: 1148 245 2120
Fragments arranged by size:
4492 2640 2326 2120 1148 677 618 427
245 218 48
NcoI C'CATG_G
Cuts at: 513 1065 4446 4782 10857 513
Size: 552 3381 336 6075 4615
Fragments arranged by size:
6075 4615 3381 552 336
NdeI CA'TA_TG
Cuts at: 387 3573 6095 9887 11355 387
Size: 3186 2522 3792 1468 3991
Fragments arranged by size:
3991 3792 3186 2522 1468
NheI G'CTAG_C
Cuts at: 1052 6584 1052
Size: 5532 9427
NotI GC'GGCC_GC
Cuts at: 12406 12406
Size: 14959
NsiI A_TGCA'T
Cuts at: 7402 8973 11550 7402
Size: 1571 2577 10811
Fragments arranged by size:
10811 2577 1571
NspI r_CATG'y
Cuts at: 1132 1229 2456 4032 4218 6201 6744 7240 8772
Size: 97 1227 1576 186 1983 543 496 1532
Cuts at: 8772 11561 12317 13862 1132
Size: 2789 756 1545 2229
Fragments arranged by size:
2789 2229 1983 1576 1545 1532 1227 756
543 496 186 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 14959
PciI A'CATG_T
Cuts at: 2452 4028 8768 13858 2452
Size: 1576 4740 5090 3553
Fragments arranged by size:
5090 4740 3553 1576
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5522 10150 1108
Size: 4414 4628 5917
Fragments arranged by size:
5917 4628 4414
PmeI GTTT'AAAC
Cuts at: 12263 12263
Size: 14959
PmlI CAC'GTG
Cuts at: 10667 10667
Size: 14959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3997 4029 9579 9611 13028 13060 14541 14573 3997
Size: 32 5550 32 3417 32 1481 32 4383
Fragments arranged by size:
5550 4383 3417 1481 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 14959
PsiI TTA'TAA
Cuts at: 2217 3804 4068 4488 9867 12529 2217
Size: 1587 264 420 5379 2662 4647
Fragments arranged by size:
5379 4647 2662 1587 420 264
PstI C_TGCA'G
Cuts at: 830 2146 4143 4213 5764 7129 8384 12311 830
Size: 1316 1997 70 1551 1365 1255 3927 3478
Fragments arranged by size:
3927 3478 1997 1551 1365 1316 1255 70
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 14959
PvuII CAG'CTG
Cuts at: 2224 6764 9404 9622 10049 2224
Size: 4540 2640 218 427 7134
Fragments arranged by size:
7134 4540 2640 427 218
SalI G'TCGA_C
Cuts at: 1936 12301 1936
Size: 10365 4594
SapI GCTCTTCn'nnn_
Cuts at: 3408 5075 7459 10767 11803 3408
Size: 1667 2384 3308 1036 6564
Fragments arranged by size:
6564 3308 2384 1667 1036
SbfI CC_TGCA'GG
Cuts at: 4143 12311 4143
Size: 8168 6791
ScaI AGT'ACT
Cuts at: 7171 10351 13207 14919 7171
Size: 3180 2856 1712 7211
Fragments arranged by size:
7211 3180 2856 1712
SexAI A'CCwGG_T
Cuts at: 9058 9058
Size: 14959
SfcI C'TryA_G
Cuts at: 826 1045 2142 3717 4097 4139 4209 5760 7125
Size: 219 1097 1575 380 42 70 1551 1365
Cuts at: 7125 7173 8380 9198 9874 12307 13442 14123 14314
Size: 48 1207 818 676 2433 1135 681 191
Cuts at: 14314 826
Size: 1471
Fragments arranged by size:
2433 1575 1551 1471 1365 1207 1135 1097
818 681 676 380 219 191 70 48
42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 14959
SmaI CCC'GGG
Cuts at: 1872 12286 1872
Size: 10414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6189 6850 10754 11824 13063 13964
Size: 197 926 4246 661 3904 1070 1239 901
Cuts at: 13964 14226 820
Size: 262 1553
Fragments arranged by size:
4246 3904 1553 1239 1070 926 901 661
262 197
SnaBI TAC'GTA
Cuts at: 493 8105 9630 12750 493
Size: 7612 1525 3120 2702
Fragments arranged by size:
7612 3120 2702 1525
SpeI A'CTAG_T
Cuts at: 152 5123 6511 6662 7358 10127 152
Size: 4971 1388 151 696 2769 4984
Fragments arranged by size:
4984 4971 2769 1388 696 151
SphI G_CATG'C
Cuts at: 1132 11561 12317 1132
Size: 10429 756 3774
Fragments arranged by size:
10429 3774 756
SspI AAT'ATT
Cuts at: 5 52 2244 3271 4441 5743 5896 6310 7095
Size: 47 2192 1027 1170 1302 153 414 785
Cuts at: 7095 9280 11509 12883 5
Size: 2185 2229 1374 2081
Fragments arranged by size:
2229 2192 2185 2081 1374 1302 1170 1027
785 414 153 47
StuI AGG'CCT
Cuts at: 1517 6719 11406 1517
Size: 5202 4687 5070
Fragments arranged by size:
5202 5070 4687
StyI C'CwwG_G
Cuts at: 513 1065 3156 4329 4446 4782 5154 10857 11814
Size: 552 2091 1173 117 336 372 5703 957
Cuts at: 11814 11906 12398 513
Size: 92 492 3074
Fragments arranged by size:
5703 3074 2091 1173 957 552 492 372
336 117 92
SwaI ATTT'AAAT
Cuts at: 8395 8395
Size: 14959
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2623 4236 10164 11950 12986 13003 13156
Size: 1255 1043 1613 5928 1786 1036 17 153
Cuts at: 13156 13341 13786 325
Size: 185 445 1498
Fragments arranged by size:
5928 1786 1613 1498 1255 1043 1036 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2447 4225 5423
Size: 274 80 33 51 1443 470 1778 1198
Cuts at: 5423 6708 6919 7169 8180 10298 10349 11597 11864
Size: 1285 211 250 1011 2118 51 1248 267
Cuts at: 11864 12161 13205 14917 96
Size: 297 1044 1712 138
Fragments arranged by size:
2118 1778 1712 1443 1285 1248 1198 1044
1011 470 297 274 267 250 211 138
80 51 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2550 2582 4136 4168 7531 7563 8499 8531 14919
Size: 32 1554 32 3363 32 936 32 6388
Cuts at: 14919 14951 2550
Size: 32 2558
Fragments arranged by size:
6388 3363 2558 1554 936 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 14959
XbaI T'CTAG_A
Cuts at: 4016 4361 7726 8297 9851 12295 4016
Size: 345 3365 571 1554 2444 6680
Fragments arranged by size:
6680 3365 2444 1554 571 345
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 7811 7811
Size: 14959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 14959
XmaI C'CCGG_G
Cuts at: 1870 12284 1870
Size: 10414 4545
XmnI GAAnn'nnTTC
Cuts at: 3943 5038 7039 13088 3943
Size: 1095 2001 6049 5814
Fragments arranged by size:
6049 5814 2001 1095
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 12767 276
Size: 53 83 186 936 11233 2468
Fragments arranged by size:
11233 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BsiWI BsmBI BstXI
EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SfiI SfoI SrfI