Restriction map for
pFN21AE0899
(Circular) (Six-base) MAPSORT of: pFN21AE0899.seq Check: 5362 from: 1 to: 8443
pFN21AE0899 8443 bp
With 182 enzymes: SgfI *
November 8, 2010 14:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2945 2945
Size: 8443
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2589 5408 6253 278
Size: 53 83 186 936 1053 2819 845 2468
Fragments arranged by size:
2819 2468 1053 936 845 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2627 5786 1937
Size: 690 3159 4594
Fragments arranged by size:
4594 3159 690
Acc65I G'GTAC_C
Cuts at: 5764 5764
Size: 8443
AclI AA'CG_TT
Cuts at: 1563 6570 6943 1563
Size: 5007 373 3063
Fragments arranged by size:
5007 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 2280 2784 5558 6506 7890 8158 1632
Size: 503 145 504 2774 948 1384 268 1917
Fragments arranged by size:
2774 1917 1384 948 504 503 268 145
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8246
AflIII A'CryG_T
Cuts at: 1203 2656 3454 7342 8319 1203
Size: 1453 798 3888 977 1327
Fragments arranged by size:
3888 1453 1327 977 798
AgeI A'CCGG_T
Cuts at: 7274 7274
Size: 8443
AhdI GACnn_n'nnGTC
Cuts at: 4135 7172 4135
Size: 3037 5406
AleI CACnn'nnGTG
Cuts at: 1166 4626 4722 4833 1166
Size: 3460 96 111 4776
Fragments arranged by size:
4776 3460 111 96
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3188 4612 1184
Size: 2004 1424 5015
Fragments arranged by size:
5015 2004 1424
AlwNI CAG_nnn'CTG
Cuts at: 3886 4939 5437 6186 7758 3886
Size: 1053 498 749 1572 4571
Fragments arranged by size:
4571 1572 1053 749 498
ApaI G_GGCC'C
Cuts at: 3210 3872 3210
Size: 662 7781
ApaLI G'TGCA_C
Cuts at: 3602 4592 4994 5090 6500 7656 3602
Size: 990 402 96 1410 1156 4389
Fragments arranged by size:
4389 1410 1156 990 402 96
ApoI r'AATT_y
Cuts at: 1493 2128 5752 5978 6130 8239 1493
Size: 635 3624 226 152 2109 1697
Fragments arranged by size:
3624 2109 1697 635 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3990 6997 160
Size: 872 2958 3007 1606
Fragments arranged by size:
3007 2958 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8443
AvaI C'yCGr_G
Cuts at: 1870 1943 3182 3619 3794 4791 5038 5768 1870
Size: 73 1239 437 175 997 247 730 4545
Fragments arranged by size:
4545 1239 997 730 437 247 175 73
BamHI G'GATC_C
Cuts at: 5042 5773 6140 5042
Size: 731 367 7345
Fragments arranged by size:
7345 731 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4919 5764 7219 618
Size: 325 217 627 3132 845 1455 1842
Fragments arranged by size:
3132 1842 1455 845 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2190 3210 3872 3939 3963 4272 4284 5268
Size: 799 1020 662 67 24 309 12 984
Cuts at: 5268 5738 5762 1391
Size: 470 24 4072
Fragments arranged by size:
4072 1020 984 799 662 470 309 67
24 24 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2589 4358 928
Size: 971 690 1769 5013
Fragments arranged by size:
5013 1769 971 690
BbvCI CC'TCA_GC
Cuts at: 4866 4866
Size: 8443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6634 6668 1903
Size: 34 4697 34 3678
Fragments arranged by size:
4697 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5266 6335 7545 5266
Size: 1069 1210 6164
Fragments arranged by size:
6164 1210 1069
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8443
BfrBI ATG'CAT
Cuts at: 2258 2258
Size: 8443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3656 5346 7054
Size: 107 122 71 631 605 1984 1690 1708
Cuts at: 7054 136
Size: 1525
Fragments arranged by size:
1984 1708 1690 1525 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5424 5589 8438 5424
Size: 165 2849 5429
Fragments arranged by size:
5429 2849 165
BlpI GC'TnA_GC
Cuts at: 5163 5860 5163
Size: 697 7746
Bme1580I G_kGCm'C
Cuts at: 1790 3210 3606 3872 4596 4998 5094 5691 6504
Size: 1420 396 266 724 402 96 597 813
Cuts at: 6504 7660 1790
Size: 1156 2573
Fragments arranged by size:
2573 1420 1156 813 724 597 402 396
266 96
BmgBI CAC'GTC
Cuts at: 1318 5686 1318
Size: 4368 4075
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3099 3213 4339
Size: 416 53 83 399 24 1682 114 1126
Cuts at: 4339 4631 5101 6174 7132 442
Size: 292 470 1073 958 1753
Fragments arranged by size:
1753 1682 1126 1073 958 470 416 399
292 114 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2373 2406 3506 4181 4214 4576 4755 5510
Size: 1134 33 1100 675 33 362 179 755
Cuts at: 5510 5695 7103 1239
Size: 185 1408 2579
Fragments arranged by size:
2579 1408 1134 1100 755 675 362 185
179 33 33
Bpu10I CC'TnA_GC
Cuts at: 2892 3741 4377 4866 5267 2892
Size: 849 636 489 401 6068
Fragments arranged by size:
6068 849 636 489 401
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3209 4310 6568 7433 7731 3209
Size: 1101 2258 865 298 3921
Fragments arranged by size:
3921 2258 1101 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4788 4794 5123 7106 882
Size: 617 3289 6 329 1983 2219
Fragments arranged by size:
3289 2219 1983 617 329 6
BsaAI yAC'GTr
Cuts at: 493 1204 2093 3457 4585 5509 6234 493
Size: 711 889 1364 1128 924 725 2702
Fragments arranged by size:
2702 1364 1128 924 889 725 711
BsaBI GATnn'nnATC
Cuts at: 8437 8437
Size: 8443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2586 3191 4419 5405
Size: 53 83 186 936 1053 605 1228 986
Cuts at: 5405 6250 6632 7267 275
Size: 845 382 635 1451
Fragments arranged by size:
1451 1228 1053 986 936 845 635 605
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3592 3781 6876 7274 7548 7695 8106 3592
Size: 189 3095 398 274 147 411 3929
Fragments arranged by size:
3929 3095 411 398 274 189 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3548 3578 4106 4136 4958
Size: 30 165 30 2145 30 528 30 822
Cuts at: 4958 4988 1178
Size: 30 4633
Fragments arranged by size:
4633 2145 822 528 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2564 2576 3343 4160 4418 4421 4883 4886
Size: 1378 12 767 817 258 3 462 3
Cuts at: 4886 5311 5428 1186
Size: 425 117 4201
Fragments arranged by size:
4201 1378 817 767 462 425 258 117
12 3 3
BseYI C'CCAG_C
Cuts at: 2162 2378 3853 3864 4531 7646 8142 2162
Size: 216 1475 11 667 3115 496 2463
Fragments arranged by size:
3115 2463 1475 667 496 216 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2973 3784 3899 5027 1163
Size: 573 1237 811 115 1128 4579
Fragments arranged by size:
4579 1237 1128 811 573 115
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2888 3432 3500 3516 5893 6654
Size: 275 186 887 544 68 16 2377 761
Cuts at: 6654 7682 1540
Size: 1028 2301
Fragments arranged by size:
2377 2301 1028 887 761 544 275 186
68 16
BsiHKAI G_wGCw'C
Cuts at: 3527 3606 3939 3963 4152 4596 4998 5094 5268
Size: 79 333 24 189 444 402 96 174
Cuts at: 5268 5500 5738 6504 6589 7660 3527
Size: 232 238 766 85 1071 4310
Fragments arranged by size:
4310 1071 766 444 402 333 238 232
189 174 96 85 79 24
BsmI GAATG_Cn'
Cuts at: 4857 5954 6047 4857
Size: 1097 93 7253
Fragments arranged by size:
7253 1097 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5396 5396
Size: 8443
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 3210 3527 3606 3872 3939 3963
Size: 399 400 1020 317 79 266 67 24
Cuts at: 3963 4152 4272 4284 4596 4998 5094 5268 5500
Size: 189 120 12 312 402 96 174 232
Cuts at: 5500 5691 5738 5762 6504 6589 7660 1391
Size: 191 47 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 1020 742 402 400 399 317
312 266 232 191 189 174 120 96
85 79 67 47 24 24 12
BspEI T'CCGG_A
Cuts at: 3781 8106 3781
Size: 4325 4118
BspHI T'CATG_A
Cuts at: 4500 4953 6332 8062 4500
Size: 453 1379 1730 4881
Fragments arranged by size:
4881 1730 1379 453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2682 2945 3228 5798 844
Size: 1838 263 283 2570 3489
Fragments arranged by size:
3489 2570 1838 283 263
BsrBI CCG'CTC
Cuts at: 1394 1823 3715 3943 6330 1394
Size: 429 1892 228 2387 3507
Fragments arranged by size:
3507 2387 1892 429 228
BsrDI GCAATG_nn'
Cuts at: 65 1248 2157 2408 3157 6938 7112 65
Size: 1183 909 251 749 3781 174 1396
Fragments arranged by size:
3781 1396 1183 909 749 251 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3858 4080 4152 4359 7087 7274 1700
Size: 110 2048 222 72 207 2728 187 2869
Fragments arranged by size:
2869 2728 2048 222 207 187 110 72
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8443
BssSI C'ACGA_G
Cuts at: 6503 7515 6503
Size: 1012 7431
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8443
BstBI TT'CG_AA
Cuts at: 7247 7247
Size: 8443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8443
BstXI CCAn_nnnn'nTGG
Cuts at: 2521 2521
Size: 8443
BstYI r'GATC_y
Cuts at: 1972 3672 4188 5042 5424 5589 5773 6140 6525
Size: 1700 516 854 382 165 184 367 385
Cuts at: 6525 6542 7994 8080 8092 8438 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1700 1452 854 516 385 382 367
346 184 165 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3683 3683
Size: 8443
BtgI C'CryG_G
Cuts at: 513 1065 2069 2829 3024 3510 3663 3927 4288
Size: 552 1004 760 195 486 153 264 361
Cuts at: 4288 5175 5451 513
Size: 887 276 3505
Fragments arranged by size:
3505 1004 887 760 552 486 361 276
264 195 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7704
Fragments arranged by size:
7704 683 56
BtsI GCAGTG_nn'
Cuts at: 3018 4594 4596 4633 5959 6753 6773 3018
Size: 1576 2 37 1326 794 20 4688
Fragments arranged by size:
4688 1576 1326 794 37 20 2
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3214 3249 529
Size: 35 2650 35 5723
Fragments arranged by size:
5723 2650 35 35
DraI TTT'AAA
Cuts at: 5747 5747
Size: 8443
DraIII CAC_nnn'GTG
Cuts at: 4294 5109 4294
Size: 815 7628
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4418 7450 809
Size: 507 3102 3032 1802
Fragments arranged by size:
3102 3032 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2841 3429 3497
Size: 54 1425 50 275 18 1011 588 68
Cuts at: 3497 3513 3660 4086 4357 5017 5890 6779 8408
Size: 16 147 426 271 660 873 889 1629
Cuts at: 8408 8
Size: 43
Fragments arranged by size:
1629 1425 1011 889 873 660 588 426
275 271 147 68 54 50 43 18
16
EagI C'GGCC_G
Cuts at: 1537 1812 3429 3497 3513 5890 1537
Size: 275 1617 68 16 2377 4090
Fragments arranged by size:
4090 2377 1617 275 68 16
EarI CTCTTCn'nnn_
Cuts at: 1353 2132 3333 3515 4376 4395 4563 6373 1353
Size: 779 1201 182 861 19 168 1810 3423
Fragments arranged by size:
3423 1810 1201 861 779 182 168 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3368 3569 3682 5378 7030 7404
Size: 1064 138 1779 201 113 1696 1652 374
Cuts at: 7404 7550 387
Size: 146 1280
Fragments arranged by size:
1779 1696 1652 1280 1064 374 201 146
138 113
EcoICRI GAG'CTC
Cuts at: 3937 3961 5266 5736 3937
Size: 24 1305 470 6644
Fragments arranged by size:
6644 1305 470 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2280 2373 2406 2784 3506 4181
Size: 393 503 145 93 33 378 722 675
Cuts at: 4181 4214 4576 4755 5510 5558 5695 6506 7103
Size: 33 362 179 755 48 137 811 597
Cuts at: 7103 7890 8158 1239
Size: 787 268 1524
Fragments arranged by size:
1524 811 787 755 722 675 597 503
393 378 362 268 179 145 137 93
48 33 33
EcoNI CCTnn'n_nnAGG
Cuts at: 4660 4660
Size: 8443
EcoO109I rG'GnC_Cy
Cuts at: 3292 3410 3424 3687 3868 3869 5660 3292
Size: 118 14 263 181 1 1791 6075
Fragments arranged by size:
6075 1791 263 181 118 14 1
EcoRI G'AATT_C
Cuts at: 2128 5752 6130 2128
Size: 3624 378 4441
Fragments arranged by size:
4441 3624 378
EcoRV GAT'ATC
Cuts at: 2436 2436
Size: 8443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2492 2492
Size: 8443
FspI TGC'GCA
Cuts at: 4058 6949 4058
Size: 2891 5552
HaeII r_GCGC'y
Cuts at: 1678 3564 3865 7590 1678
Size: 1886 301 3725 2531
Fragments arranged by size:
3725 2531 1886 301
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 3057 3089 3548
Size: 32 135 32 671 32 782 32 459
Cuts at: 3548 3580 3763 3795 4121 4153 4662 4694 5404
Size: 32 183 32 326 32 509 32 710
Cuts at: 5404 5436 5560 5592 7084 7116 7158 7190 1373
Size: 32 124 32 1492 32 42 32 2626
Fragments arranged by size:
2626 1492 782 710 671 509 459 326
183 135 124 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2628 2697 3118 5787 6033 669
Size: 988 281 690 69 421 2669 246 3079
Fragments arranged by size:
3079 2669 988 690 421 281 246 69
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8443
HpaI GTT'AAC
Cuts at: 6033 6033
Size: 8443
KpnI G_GTAC'C
Cuts at: 5768 5768
Size: 8443
MfeI C'AATT_G
Cuts at: 6042 6042
Size: 8443
MluI A'CGCG_T
Cuts at: 8319 8319
Size: 8443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5379 7557 7741 1305
Size: 4074 2178 184 2007
Fragments arranged by size:
4074 2178 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4088 5019 10
Size: 54 1425 343 2256 931 3434
Fragments arranged by size:
3434 2256 1425 931 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2617 2655 2936 3926 4505 4626 4722
Size: 648 1451 38 281 990 579 121 96
Cuts at: 4722 4833 6401 6760 6919 518
Size: 111 1568 359 159 2042
Fragments arranged by size:
2042 1568 1451 990 648 579 359 281
159 121 111 96 38
MspA1I CmG'CkG
Cuts at: 1606 2819 3113 3283 3301 3883 4213 5299 5859
Size: 1213 294 170 18 582 330 1086 560
Cuts at: 5859 6536 7684 7929 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1213 1148 1086 677 582 560 330
294 245 170 18
NaeI GCC'GGC
Cuts at: 3860 4361 3860
Size: 501 7942
NcoI C'CATG_G
Cuts at: 513 1065 3663 3927 5175 5451 513
Size: 552 2598 264 1248 276 3505
Fragments arranged by size:
3505 2598 1248 552 276 264
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8443
NgoMIV G'CCGG_C
Cuts at: 3858 4359 3858
Size: 501 7942
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8443
NotI GC'GGCC_GC
Cuts at: 5890 5890
Size: 8443
NsiI A_TGCA'T
Cuts at: 2260 2260
Size: 8443
NspI r_CATG'y
Cuts at: 1132 1229 2150 2660 2675 4009 4054 4510 5801
Size: 97 921 510 15 1334 45 456 1291
Cuts at: 5801 7346 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1334 1291 921 510 456 97
45 15
PasI CC'CwG_GG
Cuts at: 1348 4925 1348
Size: 3577 4866
PciI A'CATG_T
Cuts at: 2656 7342 2656
Size: 4686 3757
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3853 4294 5109 1108
Size: 2745 441 815 4442
Fragments arranged by size:
4442 2745 815 441
PfoI T'CCnGG_A
Cuts at: 4191 5256 4191
Size: 1065 7378
PmeI GTTT'AAAC
Cuts at: 5747 5747
Size: 8443
PmlI CAC'GTG
Cuts at: 3457 4585 5509 3457
Size: 1128 924 6391
Fragments arranged by size:
6391 1128 924
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6512 6544 8025 8057 6512
Size: 32 1481 32 6898
Fragments arranged by size:
6898 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5660 5660
Size: 8443
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8443
PsiI TTA'TAA
Cuts at: 6013 6013
Size: 8443
PspOMI G'GGCC_C
Cuts at: 3206 3868 3206
Size: 662 7781
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2073 2105 2073
Size: 32 8411
PstI C_TGCA'G
Cuts at: 830 3300 4098 4935 5397 5795 830
Size: 2470 798 837 462 398 3478
Fragments arranged by size:
3478 2470 837 798 462 398
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8443
PvuII CAG'CTG
Cuts at: 3283 3301 5299 3283
Size: 18 1998 6427
Fragments arranged by size:
6427 1998 18
RsrII CG'GwC_CG
Cuts at: 3733 3733
Size: 8443
SacI G_AGCT'C
Cuts at: 3939 3963 5268 5738 3939
Size: 24 1305 470 6644
Fragments arranged by size:
6644 1305 470 24
SalI G'TCGA_C
Cuts at: 1936 2626 5785 1936
Size: 690 3159 4594
Fragments arranged by size:
4594 3159 690
SapI GCTCTTCn'nnn_
Cuts at: 3515 3515
Size: 8443
SbfI CC_TGCA'GG
Cuts at: 5795 5795
Size: 8443
ScaI AGT'ACT
Cuts at: 4553 6691 8403 4553
Size: 2138 1712 4593
Fragments arranged by size:
4593 2138 1712
SfcI C'TryA_G
Cuts at: 826 1045 3296 4094 4931 5393 5791 6926 7607
Size: 219 2251 798 837 462 398 1135 681
Cuts at: 7607 7798 826
Size: 191 1471
Fragments arranged by size:
2251 1471 1135 837 798 681 462 398
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8443
SgrAI Cr'CCGG_yG
Cuts at: 1700 4152 1700
Size: 2452 5991
SmaI CCC'GGG
Cuts at: 1872 5040 5770 1872
Size: 3168 730 4545
Fragments arranged by size:
4545 3168 730
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3224 4325 4791 6547 7448 7710
Size: 197 926 1281 1101 466 1756 901 262
Cuts at: 7710 820
Size: 1553
Fragments arranged by size:
1756 1553 1281 1101 926 901 466 262
197
SnaBI TAC'GTA
Cuts at: 493 2093 6234 493
Size: 1600 4141 2702
Fragments arranged by size:
4141 2702 1600
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8443
SphI G_CATG'C
Cuts at: 1132 2150 4009 5801 1132
Size: 1018 1859 1792 3774
Fragments arranged by size:
3774 1859 1792 1018
SspI AAT'ATT
Cuts at: 5 52 4759 6367 5
Size: 47 4707 1608 2081
Fragments arranged by size:
4707 2081 1608 47
StuI AGG'CCT
Cuts at: 1517 4103 1517
Size: 2586 5857
StyI C'CwwG_G
Cuts at: 513 1065 2191 2844 3663 3696 3927 5136 5175
Size: 552 1126 653 819 33 231 1209 39
Cuts at: 5175 5451 5882 513
Size: 276 431 3074
Fragments arranged by size:
3074 1209 1126 819 653 552 431 276
231 39 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2874 2876 2939 3721 5120 6470 6487
Size: 1255 1294 2 63 782 1399 1350 17
Cuts at: 6487 6640 6825 7270 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1399 1350 1294 1255 782 445 185
153 63 17 2
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3015 4551 6689
Size: 274 80 33 51 1443 1038 1536 2138
Cuts at: 6689 8401 96
Size: 1712 138
Fragments arranged by size:
2138 1712 1536 1443 1038 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4958 4990 5034 5066 8403 8435 4958
Size: 32 44 32 3337 32 4966
Fragments arranged by size:
4966 3337 44 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8443
XbaI T'CTAG_A
Cuts at: 5779 5779
Size: 8443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3067 3331 4352 3067
Size: 264 1021 7158
Fragments arranged by size:
7158 1021 264
XhoI C'TCGA_G
Cuts at: 1943 4791 1943
Size: 2848 5595
XmaI C'CCGG_G
Cuts at: 1870 5038 5768 1870
Size: 3168 730 4545
Fragments arranged by size:
4545 3168 730
XmnI GAAnn'nnTTC
Cuts at: 2120 2472 2715 4310 6572 2120
Size: 352 243 1595 2262 3991
Fragments arranged by size:
3991 2262 1595 352 243
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2587 5406 6251 276
Size: 53 83 186 936 1053 2819 845 2468
Fragments arranged by size:
2819 2468 1053 936 845 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BstZ17I FseI FspAI KasI NarI NruI PacI PspXI
SacII SanDI SexAI SfiI SfoI SrfI SwaI