Restriction map for
pFN21AE0898
(Circular) (Six-base) MAPSORT of: pFN21AE0898.seq Check: 3670 from: 1 to: 8812
pFN21AE0898 8812 bp
With 182 enzymes: SgfI *
September 16, 2010 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3776 3776
Size: 8812
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6622 278
Size: 53 83 186 936 5086 2468
Fragments arranged by size:
5086 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6155 1937
Size: 4218 4594
Acc65I G'GTAC_C
Cuts at: 3817 6133 3817
Size: 2316 6496
AclI AA'CG_TT
Cuts at: 1563 4177 6111 6939 7312 1563
Size: 2614 1934 828 373 3063
Fragments arranged by size:
3063 2614 1934 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2541 3078 3276 3576 5115 6875 8259 8527
Size: 909 537 198 300 1539 1760 1384 268
Cuts at: 8527 1632
Size: 1917
Fragments arranged by size:
1917 1760 1539 1384 909 537 300 268
198
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8615
AflIII A'CryG_T
Cuts at: 1203 5038 7711 8688 1203
Size: 3835 2673 977 1327
Fragments arranged by size:
3835 2673 1327 977
AgeI A'CCGG_T
Cuts at: 7643 7643
Size: 8812
AhdI GACnn_n'nnGTC
Cuts at: 7541 7541
Size: 8812
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8812
AlwNI CAG_nnn'CTG
Cuts at: 3319 6555 8127 3319
Size: 3236 1572 4004
Fragments arranged by size:
4004 3236 1572
ApaLI G'TGCA_C
Cuts at: 6869 8025 6869
Size: 1156 7656
ApoI r'AATT_y
Cuts at: 1493 2199 2537 2570 2746 2774 2900 3022 3100
Size: 706 338 33 176 28 126 122 78
Cuts at: 3100 3580 3970 4164 4226 4263 4361 5155 6121
Size: 480 390 194 62 37 98 794 966
Cuts at: 6121 6347 6499 8608 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 966 794 706 480 390 338
226 194 176 152 126 122 98 78
62 37 33 28
AseI AT'TA_AT
Cuts at: 160 1032 2274 4392 7366 160
Size: 872 1242 2118 2974 1606
Fragments arranged by size:
2974 2118 1606 1242 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8812
AvaI C'yCGr_G
Cuts at: 1870 1943 3563 4005 6137 1870
Size: 73 1620 442 2132 4545
Fragments arranged by size:
4545 2132 1620 442 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3671 3704 3801 3834 3671
Size: 33 97 33 8649
Fragments arranged by size:
8649 97 33 33
BamHI G'GATC_C
Cuts at: 6142 6509 6142
Size: 367 8445
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3817 5270 6133 7588 618
Size: 325 217 627 2030 1453 863 1455 1842
Fragments arranged by size:
2030 1842 1455 1453 863 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2178 6131 1391
Size: 787 3953 4072
Fragments arranged by size:
4072 3953 787
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2362 2934 4070 5924 928
Size: 971 463 572 1136 1854 3816
Fragments arranged by size:
3816 1854 1136 971 572 463
BbvCI CC'TCA_GC
Cuts at: 5127 5127
Size: 8812
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7003 7037 1903
Size: 34 5066 34 3678
Fragments arranged by size:
5066 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6704 7914 6704
Size: 1210 7602
BclI T'GATC_A
Cuts at: 1548 2307 5139 1548
Size: 759 2832 5221
Fragments arranged by size:
5221 2832 759
BfrBI ATG'CAT
Cuts at: 5426 5426
Size: 8812
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2108 7423 136
Size: 107 122 71 631 605 436 5315 1525
Fragments arranged by size:
5315 1525 631 605 436 122 107 71
BglII A'GATC_T
Cuts at: 5368 8807 5368
Size: 3439 5373
BlpI GC'TnA_GC
Cuts at: 3312 6229 3312
Size: 2917 5895
Bme1580I G_kGCm'C
Cuts at: 1790 6873 8029 1790
Size: 5083 1156 2573
Fragments arranged by size:
5083 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8812
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4436 5288 6543
Size: 416 53 83 399 24 3019 852 1255
Cuts at: 6543 7501 442
Size: 958 1753
Fragments arranged by size:
3019 1753 1255 958 852 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2019 2152 3425 3981 4473 4749 7472 1239
Size: 780 133 1273 556 492 276 2723 2579
Fragments arranged by size:
2723 2579 1273 780 556 492 276 133
Bpu10I CC'TnA_GC
Cuts at: 2883 4055 5127 2883
Size: 1172 1072 6568
Fragments arranged by size:
6568 1172 1072
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6937 7802 8100 6937
Size: 865 298 7649
Fragments arranged by size:
7649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7475 882
Size: 617 5976 2219
Fragments arranged by size:
5976 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3467 5039 6603 493
Size: 711 2263 1572 1564 2702
Fragments arranged by size:
2702 2263 1572 1564 711
BsaBI GATnn'nnATC
Cuts at: 4470 5575 8806 4470
Size: 1105 3231 4476
Fragments arranged by size:
4476 3231 1105
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6619 7001 7636 275
Size: 53 83 186 936 5086 382 635 1451
Fragments arranged by size:
5086 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3993 7245 7643 7917 8064 8475 3993
Size: 3252 398 274 147 411 4330
Fragments arranged by size:
4330 3252 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8587
Fragments arranged by size:
8587 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2571 2780 5115 1186
Size: 1385 209 2335 4883
Fragments arranged by size:
4883 2335 1385 209
BseYI C'CCAG_C
Cuts at: 2947 4949 5598 8015 8511 2947
Size: 2002 649 2417 496 3248
Fragments arranged by size:
3248 2417 2002 649 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3591 1163
Size: 573 1855 6384
Fragments arranged by size:
6384 1855 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2043 6262 7023 8051 1540
Size: 275 186 42 4219 761 1028 2301
Fragments arranged by size:
4219 2301 1028 761 275 186 42
BsiHKAI G_wGCw'C
Cuts at: 4459 6873 6958 8029 4459
Size: 2414 85 1071 5242
Fragments arranged by size:
5242 2414 1071 85
BsmI GAATG_Cn'
Cuts at: 3529 4714 4748 6323 6416 3529
Size: 1185 34 1575 93 5925
Fragments arranged by size:
5925 1575 1185 93 34
BsmBI CGTCTCn'nnnn_
Cuts at: 4799 4898 5741 4799
Size: 99 843 7870
Fragments arranged by size:
7870 843 99
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 4459 6131 6873 6958 8029 1391
Size: 399 388 2281 1672 742 85 1071 2174
Fragments arranged by size:
2281 2174 1672 1071 742 399 388 85
BspEI T'CCGG_A
Cuts at: 3993 8475 3993
Size: 4482 4330
BspHI T'CATG_A
Cuts at: 3088 4117 6701 8431 3088
Size: 1029 2584 1730 3469
Fragments arranged by size:
3469 2584 1730 1029
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3776 6167 844
Size: 2932 2391 3489
Fragments arranged by size:
3489 2932 2391
BsrBI CCG'CTC
Cuts at: 1394 1823 6699 1394
Size: 429 4876 3507
Fragments arranged by size:
4876 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2609 5152 5157 7307 7481 65
Size: 1183 1361 2543 5 2150 174 1396
Fragments arranged by size:
2543 2150 1396 1361 1183 174 5
BsrFI r'CCGG_y
Cuts at: 1700 1810 2137 2162 7456 7643 1700
Size: 110 327 25 5294 187 2869
Fragments arranged by size:
5294 2869 327 187 110 25
BsrGI T'GTAC_A
Cuts at: 96 4037 4983 96
Size: 3941 946 3925
Fragments arranged by size:
3941 3925 946
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8812
BssSI C'ACGA_G
Cuts at: 6872 7884 6872
Size: 1012 7800
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5372 794
Size: 4578 4234
BstBI TT'CG_AA
Cuts at: 7616 7616
Size: 8812
BstEII G'GTnAC_C
Cuts at: 1184 2462 1184
Size: 1278 7534
BstYI r'GATC_y
Cuts at: 1972 3559 3990 4546 5119 5368 5952 6142 6509
Size: 1587 431 556 573 249 584 190 367
Cuts at: 6509 6894 6911 8363 8449 8461 8807 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1587 1452 584 573 556 431 385
367 346 249 190 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 5391 513
Size: 552 939 39 3348 3934
Fragments arranged by size:
3934 3348 939 552 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8073
Fragments arranged by size:
8073 683 56
BtsI GCAGTG_nn'
Cuts at: 4246 6328 7122 7142 4246
Size: 2082 794 20 5916
Fragments arranged by size:
5916 2082 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5329 5364 5694 5729 529
Size: 35 4765 35 330 35 3612
Fragments arranged by size:
4765 3612 330 35 35 35
DraI TTT'AAA
Cuts at: 2848 3730 4470 6116 2848
Size: 882 740 1646 5544
Fragments arranged by size:
5544 1646 882 740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 7819 809
Size: 507 4010 2493 1802
Fragments arranged by size:
4010 2493 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2139 5388
Size: 54 1425 50 275 18 210 99 3249
Cuts at: 5388 6259 7148 8777 8
Size: 871 889 1629 43
Fragments arranged by size:
3249 1629 1425 889 871 275 210 99
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2040 6259 1537
Size: 275 228 4219 4090
Fragments arranged by size:
4219 4090 275 228
EarI CTCTTCn'nnn_
Cuts at: 1353 2538 2631 2757 3057 4365 4939 6742 1353
Size: 1185 93 126 300 1308 574 1803 3423
Fragments arranged by size:
3423 1803 1308 1185 574 300 126 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5411 7399 7773 7919 387
Size: 1064 138 3822 1988 374 146 1280
Fragments arranged by size:
3822 1988 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2019 2152 2541 3078 3276 3425 3576
Size: 393 387 133 389 537 198 149 151
Cuts at: 3576 3981 4473 4749 5115 6875 7472 8259 8527
Size: 405 492 276 366 1760 597 787 268
Cuts at: 8527 1239
Size: 1524
Fragments arranged by size:
1760 1524 787 597 537 492 405 393
389 387 366 276 268 198 151 149
133
EcoO109I rG'GnC_Cy
Cuts at: 2641 5965 2641
Size: 3324 5488
EcoRI G'AATT_C
Cuts at: 2746 3970 6121 6499 2746
Size: 1224 2151 378 5059
Fragments arranged by size:
5059 2151 1224 378
EcoRV GAT'ATC
Cuts at: 3986 3986
Size: 8812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3860 4094 5387 3860
Size: 234 1293 7285
Fragments arranged by size:
7285 1293 234
FseI GG_CCGG'CC
Cuts at: 2141 2141
Size: 8812
FspI TGC'GCA
Cuts at: 7318 7318
Size: 8812
HaeII r_GCGC'y
Cuts at: 1678 4939 7959 1678
Size: 3261 3020 2531
Fragments arranged by size:
3261 3020 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2950 2982 3175 3207 3975
Size: 32 135 32 1378 32 193 32 768
Cuts at: 3975 3988 4007 4020 4649 4681 5090 5122 7453
Size: 13 19 13 629 32 409 32 2331
Cuts at: 7453 7485 7527 7559 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2331 1378 768 629 409 193 135
42 32 32 32 32 32 32 32
32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 5520 6156 6402 669
Size: 988 281 3582 636 246 3079
Fragments arranged by size:
3582 3079 988 636 281 246
HindIII A'AGCT_T
Cuts at: 748 6032 748
Size: 5284 3528
HpaI GTT'AAC
Cuts at: 5520 6402 5520
Size: 882 7930
KpnI G_GTAC'C
Cuts at: 3821 6137 3821
Size: 2316 6496
MfeI C'AATT_G
Cuts at: 3744 5346 6411 3744
Size: 1602 1065 6145
Fragments arranged by size:
6145 1602 1065
MluI A'CGCG_T
Cuts at: 8688 8688
Size: 8812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3104 7926 8110 1305
Size: 1799 4822 184 2007
Fragments arranged by size:
4822 2007 1799 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5390 10
Size: 54 1425 343 3558 3432
Fragments arranged by size:
3558 3432 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6770 7129 7288 518
Size: 648 5604 359 159 2042
Fragments arranged by size:
5604 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2045 2147 2153 2159 3050 5598 5998
Size: 433 6 102 6 6 891 2548 400
Cuts at: 5998 6228 6905 8053 8298 1606
Size: 230 677 1148 245 2120
Fragments arranged by size:
2548 2120 1148 891 677 433 400 245
230 102 6 6 6
NaeI GCC'GGC
Cuts at: 2139 2164 2139
Size: 25 8787
NcoI C'CATG_G
Cuts at: 513 1065 2004 5391 513
Size: 552 939 3387 3934
Fragments arranged by size:
3934 3387 939 552
NdeI CA'TA_TG
Cuts at: 387 2632 387
Size: 2245 6567
NgoMIV G'CCGG_C
Cuts at: 2137 2162 2137
Size: 25 8787
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8812
NotI GC'GGCC_GC
Cuts at: 2040 6259 2040
Size: 4219 4593
NsiI A_TGCA'T
Cuts at: 5428 5428
Size: 8812
NspI r_CATG'y
Cuts at: 1132 1229 3307 4463 4511 6170 7715 1132
Size: 97 2078 1156 48 1659 1545 2229
Fragments arranged by size:
2229 2078 1659 1545 1156 97 48
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8812
PciI A'CATG_T
Cuts at: 7711 7711
Size: 8812
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8812
PfoI T'CCnGG_A
Cuts at: 4765 4765
Size: 8812
PmeI GTTT'AAAC
Cuts at: 6116 6116
Size: 8812
PmlI CAC'GTG
Cuts at: 3467 3467
Size: 8812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6881 6913 8394 8426 6881
Size: 32 1481 32 7267
Fragments arranged by size:
7267 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2641 2641
Size: 8812
PshAI GACnn'nnGTC
Cuts at: 1613 4910 1613
Size: 3297 5515
PsiI TTA'TAA
Cuts at: 6382 6382
Size: 8812
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4098 4130 5071 5103 4098
Size: 32 941 32 7807
Fragments arranged by size:
7807 941 32 32
PstI C_TGCA'G
Cuts at: 830 3049 4747 5314 6078 6164 830
Size: 2219 1698 567 764 86 3478
Fragments arranged by size:
3478 2219 1698 764 567 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8812
PvuII CAG'CTG
Cuts at: 3050 5598 5998 3050
Size: 2548 400 5864
Fragments arranged by size:
5864 2548 400
SacII CC_GC'GG
Cuts at: 2046 2046
Size: 8812
SalI G'TCGA_C
Cuts at: 1936 6154 1936
Size: 4218 4594
SapI GCTCTTCn'nnn_
Cuts at: 2538 4939 2538
Size: 2401 6411
SbfI CC_TGCA'GG
Cuts at: 6164 6164
Size: 8812
ScaI AGT'ACT
Cuts at: 3811 7060 8772 3811
Size: 3249 1712 3851
Fragments arranged by size:
3851 3249 1712
SexAI A'CCwGG_T
Cuts at: 4022 4022
Size: 8812
SfcI C'TryA_G
Cuts at: 826 1045 2994 3045 4743 5310 6074 6160 7295
Size: 219 1949 51 1698 567 764 86 1135
Cuts at: 7295 7976 8167 826
Size: 681 191 1471
Fragments arranged by size:
1949 1698 1471 1135 764 681 567 219
191 86 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8812
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8812
SmaI CCC'GGG
Cuts at: 1872 6139 1872
Size: 4267 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4005 6916 7817 8079 820
Size: 197 926 2062 2911 901 262 1553
Fragments arranged by size:
2911 2062 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6603 493
Size: 6110 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8812
SphI G_CATG'C
Cuts at: 1132 3307 4511 6170 1132
Size: 2175 1204 1659 3774
Fragments arranged by size:
3774 2175 1659 1204
SspI AAT'ATT
Cuts at: 5 52 4352 4600 4798 6736 5
Size: 47 4300 248 198 1938 2081
Fragments arranged by size:
4300 2081 1938 248 198 47
StuI AGG'CCT
Cuts at: 1517 2566 1517
Size: 1049 7763
StyI C'CwwG_G
Cuts at: 513 1065 2004 2478 3615 5391 5755 6251 513
Size: 552 939 474 1137 1776 364 496 3074
Fragments arranged by size:
3074 1776 1137 939 552 496 474 364
SwaI ATTT'AAAT
Cuts at: 4470 4470
Size: 8812
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5790 6839 6856 7009 7194 7639 325
Size: 1255 4210 1049 17 153 185 445 1498
Fragments arranged by size:
4210 1498 1255 1049 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3809 4037 4330
Size: 274 80 33 51 1443 1832 228 293
Cuts at: 4330 4488 4983 5659 5770 7058 8770 96
Size: 158 495 676 111 1288 1712 138
Fragments arranged by size:
1832 1712 1443 1288 676 495 293 274
228 158 138 111 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8772 8804 8772
Size: 32 8780
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8812
XbaI T'CTAG_A
Cuts at: 6148 6148
Size: 8812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4946 4946
Size: 8812
XhoI C'TCGA_G
Cuts at: 1943 4005 1943
Size: 2062 6750
XmaI C'CCGG_G
Cuts at: 1870 6137 1870
Size: 4267 4545
XmnI GAAnn'nnTTC
Cuts at: 2347 3207 3970 5931 6941 2347
Size: 860 763 1961 1010 4218
Fragments arranged by size:
4218 1961 1010 860 763
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6620 276
Size: 53 83 186 936 5086 2468
Fragments arranged by size:
5086 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FspAI KasI
NarI NruI PacI PspOMI PspXI RsrII SacI SanDI
SfiI SfoI SrfI