Restriction map for
pFN21AE0890
(Circular) (Six-base) MAPSORT of: pFN21AE0890.seq Check: 1435 from: 1 to: 5644
pFN21AE0890 5644 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3707 5091 5359 1632
Size: 2075 1384 268 1917
Fragments arranged by size:
2075 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 4543 5520 1203
Size: 3340 977 1327
Fragments arranged by size:
3340 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 2618 4373 2618
Size: 1755 3889
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AlwNI CAG_nnn'CTG
Cuts at: 3387 4959 3387
Size: 1572 4072
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2953 3179 3331 5440 1493
Size: 1460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2969 1870
Size: 73 1026 4545
Fragments arranged by size:
4545 1026 73
BamHI G'GATC_C
Cuts at: 2451 2974 3341 2451
Size: 523 367 4754
Fragments arranged by size:
4754 523 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2965 4420 618
Size: 325 217 627 1178 1455 1842
Fragments arranged by size:
1842 1455 1178 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2905 2963 1391
Size: 1514 58 4072
Fragments arranged by size:
4072 1514 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4673
BbvCI CC'TCA_GC
Cuts at: 2192 2192
Size: 5644
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 2112 2844 1548
Size: 564 732 4348
Fragments arranged by size:
4348 732 564
BfrBI ATG'CAT
Cuts at: 2643 2643
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 3705 4861 1790
Size: 1915 1156 2573
Fragments arranged by size:
2573 1915 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2788 2927 4304 1239
Size: 1549 139 1377 2579
Fragments arranged by size:
2579 1549 1377 139
Bpu10I CC'TnA_GC
Cuts at: 2192 2192
Size: 5644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 2666 2925 3769 4634 4932 2543
Size: 123 259 844 865 298 3255
Fragments arranged by size:
3255 865 844 298 259 123
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 3833 4468 275
Size: 53 83 186 936 1918 382 635 1451
Fragments arranged by size:
1918 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5419
Fragments arranged by size:
5419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2477 1186
Size: 847 444 4353
Fragments arranged by size:
4353 847 444
BseYI C'CCAG_C
Cuts at: 2122 2496 4847 5343 2122
Size: 374 2351 496 2423
Fragments arranged by size:
2423 2351 496 374
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2368 3094 3855 4883 1540
Size: 275 186 367 726 761 1028 2301
Fragments arranged by size:
2301 1028 761 726 367 275 186
BsiHKAI G_wGCw'C
Cuts at: 3705 3790 4861 3705
Size: 85 1071 4488
Fragments arranged by size:
4488 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2905 2963 3705 3790 4861 1391
Size: 399 1115 58 742 85 1071 2174
Fragments arranged by size:
2174 1115 1071 742 399 85 58
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 2409 3533 5263 2409
Size: 1124 1730 2790
Fragments arranged by size:
2790 1730 1124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2999 844
Size: 1295 860 3489
Fragments arranged by size:
3489 1295 860
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2852 4139 4313 65
Size: 1183 1604 1287 174 1396
Fragments arranged by size:
1604 1396 1287 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 2835 96
Size: 2739 2905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 2671 1184
Size: 1487 4157
BstYI r'GATC_y
Cuts at: 1972 2451 2974 3341 3726 3743 5195 5281 5293
Size: 479 523 367 385 17 1452 86 12
Cuts at: 5293 5639 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 523 479 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2879 2879
Size: 5644
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5092
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 2032 3160 3954 3974 2032
Size: 1128 794 20 3702
Fragments arranged by size:
3702 1128 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2063 2376 2659
Size: 54 1425 50 275 18 233 313 283
Cuts at: 2659 3091 3980 5609 8
Size: 432 889 1629 43
Fragments arranged by size:
1629 1425 889 432 313 283 275 233
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 2921 3574 1353
Size: 650 918 653 3423
Fragments arranged by size:
3423 918 653 650
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2788 2927 3707 4304 5091 5359 1239
Size: 393 1156 139 780 597 787 268 1524
Fragments arranged by size:
1524 1156 787 780 597 393 268 139
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 5644
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 4791 1678
Size: 3113 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 2604 2636 4285
Size: 32 135 32 430 32 570 32 1649
Cuts at: 4285 4317 4359 4391 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1649 570 430 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2712 748
Size: 1964 3680
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4758 4942 1305
Size: 3453 184 2007
Fragments arranged by size:
3453 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2065 2378 2661 10
Size: 54 1425 343 233 313 283 2993
Fragments arranged by size:
2993 1425 343 313 283 233 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2354 2408 2638 3602 3961 4120
Size: 648 1011 177 54 230 964 359 159
Cuts at: 4120 518
Size: 2042
Fragments arranged by size:
2042 1011 964 648 359 230 177 159
54
MspA1I CmG'CkG
Cuts at: 1606 2500 3060 3737 4885 5130 1606
Size: 894 560 677 1148 245 2120
Fragments arranged by size:
2120 1148 894 677 560 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5092
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NsiI A_TGCA'T
Cuts at: 2645 2645
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 2107 2260 3002 4547 1132
Size: 97 878 153 742 1545 2229
Fragments arranged by size:
2229 1545 878 742 153 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PfoI T'CCnGG_A
Cuts at: 2046 2447 2046
Size: 401 5243
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2996 830
Size: 2166 3478
PvuI CG_AT'CG
Cuts at: 2001 2368 2001
Size: 367 5277
PvuII CAG'CTG
Cuts at: 2500 2500
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SfcI C'TryA_G
Cuts at: 826 1045 2992 4127 4808 4999 826
Size: 219 1947 1135 681 191 1471
Fragments arranged by size:
1947 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 2681 2940 3748 4649 4911
Size: 197 926 615 123 259 808 901 262
Cuts at: 4911 820
Size: 1553
Fragments arranged by size:
1553 926 901 808 615 262 259 197
123
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 2434 152
Size: 2282 3362
SphI G_CATG'C
Cuts at: 1132 2260 3002 1132
Size: 1128 742 3774
Fragments arranged by size:
3774 1128 742
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 3083 513
Size: 552 2018 3074
Fragments arranged by size:
3074 2018 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3671 3688 3841 4026 4471 325
Size: 1255 2091 17 153 185 445 1498
Fragments arranged by size:
2091 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2835 3890 5602
Size: 274 80 33 51 1443 858 1055 1712
Cuts at: 5602 96
Size: 138
Fragments arranged by size:
1712 1443 1055 858 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2332 2980 2332
Size: 648 4996
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 2864 3773 2864
Size: 909 4735
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI