Restriction map for
pFN21AE0889
(Circular) (Six-base) MAPSORT of: pFN21AE0889.seq Check: 8751 from: 1 to: 5671
pFN21AE0889 5671 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2811 3734 5118 5386 1632
Size: 1179 923 1384 268 1917
Fragments arranged by size:
1917 1384 1179 923 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 4570 5547 1203
Size: 3367 977 1327
Fragments arranged by size:
3367 1327 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5671
AlwNI CAG_nnn'CTG
Cuts at: 3414 4986 3414
Size: 1572 4099
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2980 3206 3358 5467 1493
Size: 1487 226 152 2109 1697
Fragments arranged by size:
2109 1697 1487 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2996 1870
Size: 73 1053 4545
Fragments arranged by size:
4545 1053 73
BamHI G'GATC_C
Cuts at: 2729 3001 3368 2729
Size: 272 367 5032
Fragments arranged by size:
5032 367 272
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2549 2992 4447 618
Size: 325 217 627 762 443 1455 1842
Fragments arranged by size:
1842 1455 762 627 443 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2905 2990 1391
Size: 621 893 85 4072
Fragments arranged by size:
4072 893 621 85
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 1903
Size: 34 1925 34 3678
Fragments arranged by size:
3678 1925 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5671
BfrBI ATG'CAT
Cuts at: 2295 2295
Size: 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4282 136
Size: 107 122 71 631 605 2610 1525
Fragments arranged by size:
2610 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 3088 3088
Size: 5671
Bme1580I G_kGCm'C
Cuts at: 1790 2554 3732 4888 1790
Size: 764 1178 1156 2573
Fragments arranged by size:
2573 1178 1156 764
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3402 4360 442
Size: 416 53 83 399 24 1985 958 1753
Fragments arranged by size:
1985 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2666 4331 1239
Size: 1427 1665 2579
Fragments arranged by size:
2579 1665 1427
Bpu10I CC'TnA_GC
Cuts at: 2192 2192
Size: 5671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 2938 3796 4661 4959 2543
Size: 395 858 865 298 3255
Fragments arranged by size:
3255 865 858 395 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2780 4334 882
Size: 617 1281 1554 2219
Fragments arranged by size:
2219 1554 1281 617
BsaAI yAC'GTr
Cuts at: 493 1204 3462 493
Size: 711 2258 2702
Fragments arranged by size:
2702 2258 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5446
Fragments arranged by size:
5446 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2477 1186
Size: 847 444 4380
Fragments arranged by size:
4380 847 444
BseYI C'CCAG_C
Cuts at: 2122 2441 2496 4874 5370 2122
Size: 319 55 2378 496 2423
Fragments arranged by size:
2423 2378 496 319 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5098
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3882 4910 1540
Size: 275 186 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2473 3732 3817 4888 2473
Size: 1259 85 1071 3256
Fragments arranged by size:
3256 1259 1071 85
BsmI GAATG_Cn'
Cuts at: 3182 3275 3182
Size: 93 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2473 2554 2905 2990 3732 3817
Size: 399 222 461 81 351 85 742 85
Cuts at: 3817 4888 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 461 399 351 222 85
85 81
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 2409 3560 5290 2409
Size: 1151 1730 2790
Fragments arranged by size:
2790 1730 1151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 3026 844
Size: 1295 887 3489
Fragments arranged by size:
3489 1295 887
BsrBI CCG'CTC
Cuts at: 1394 1823 3558 1394
Size: 429 1735 3507
Fragments arranged by size:
3507 1735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4166 4340 65
Size: 1183 2918 174 1396
Fragments arranged by size:
2918 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4315 4502 1700
Size: 110 2505 187 2869
Fragments arranged by size:
2869 2505 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5671
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 2671 1184
Size: 1487 4184
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2122
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 2729 3001 3368 3753 3770 5222 5308 5320
Size: 757 272 367 385 17 1452 86 12
Cuts at: 5320 5666 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 757 385 367 346 272 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2115 513
Size: 552 1050 4069
Fragments arranged by size:
4069 1050 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2440 487
Size: 683 56 1214 3718
Fragments arranged by size:
3718 1214 683 56
BtsI GCAGTG_nn'
Cuts at: 2940 3187 3981 4001 2940
Size: 247 794 20 4610
Fragments arranged by size:
4610 794 247 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2063 2376 3118
Size: 54 1425 50 275 18 233 313 742
Cuts at: 3118 4007 5636 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 742 313 275 233 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3601 1353
Size: 2248 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4258 4632 4778 387
Size: 1064 138 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2666 2811 3734 4331 5118 5386 1239
Size: 393 1034 145 923 597 787 268 1524
Fragments arranged by size:
1524 1034 923 787 597 393 268 145
EcoO109I rG'GnC_Cy
Cuts at: 2445 2445
Size: 5671
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 4818 1678
Size: 3140 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2721 2753 4312 4344 4386
Size: 32 135 32 1149 32 1559 32 42
Cuts at: 4386 4418 1373
Size: 32 2626
Fragments arranged by size:
2626 1559 1149 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3261 669
Size: 988 281 1077 246 3079
Fragments arranged by size:
3079 1077 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4785 4969 1305
Size: 3480 184 2007
Fragments arranged by size:
3480 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3303
Fragments arranged by size:
3303 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2354 2408 2435 2638 2852 3629
Size: 648 1011 177 54 27 203 214 777
Cuts at: 3629 3988 4147 518
Size: 359 159 2042
Fragments arranged by size:
2042 1011 777 648 359 214 203 177
159 54 27
MspA1I CmG'CkG
Cuts at: 1606 3087 3764 4912 5157 1606
Size: 1481 677 1148 245 2120
Fragments arranged by size:
2120 1481 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5119
NdeI CA'TA_TG
Cuts at: 387 2362 2705 387
Size: 1975 343 3353
Fragments arranged by size:
3353 1975 343
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NsiI A_TGCA'T
Cuts at: 2297 2297
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 2107 2260 3029 4574 1132
Size: 97 878 153 769 1545 2229
Fragments arranged by size:
2229 1545 878 769 153 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5671
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2445 2445
Size: 5671
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PstI C_TGCA'G
Cuts at: 830 3023 830
Size: 2193 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 3019 4154 4835 5026 826
Size: 219 1974 1135 681 191 1471
Fragments arranged by size:
1974 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 2917 3775 4676 4938 820
Size: 197 926 615 359 858 901 262 1553
Fragments arranged by size:
1553 926 901 858 615 359 262 197
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5671
StyI C'CwwG_G
Cuts at: 513 1065 2545 2697 3110 513
Size: 552 1480 152 413 3074
Fragments arranged by size:
3074 1480 552 413 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3698 3715 3868 4053 4498 325
Size: 1255 2118 17 153 185 445 1498
Fragments arranged by size:
2118 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3917 5629
Size: 274 80 33 51 1443 204 1736 1712
Cuts at: 5629 96
Size: 138
Fragments arranged by size:
1736 1712 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5631 5663 5631
Size: 32 5639
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 2864 3800 2864
Size: 936 4735
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI