Restriction map for
pFN21AE0888
(Circular) (Six-base) MAPSORT of: pFN21AE0888.seq Check: 7205 from: 1 to: 5770
pFN21AE0888 5770 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2492 2492
Size: 5770
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3113 1937
Size: 1176 4594
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 3833 5217 5485 1632
Size: 947 1254 1384 268 1917
Fragments arranged by size:
1917 1384 1254 947 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 4669 5646 1203
Size: 3466 977 1327
Fragments arranged by size:
3466 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 2744 1166
Size: 1578 4192
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 3513 5085 3513
Size: 1572 4198
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 2036 3079 3305 3457 5566 1493
Size: 543 1043 226 152 2109 1697
Fragments arranged by size:
2109 1697 1043 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 2697 3095 1870
Size: 73 754 398 4545
Fragments arranged by size:
4545 754 398 73
AvrII C'CTAG_G
Cuts at: 2942 2942
Size: 5770
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2624 3091 4546 618
Size: 325 217 627 837 467 1455 1842
Fragments arranged by size:
1842 1455 837 627 467 325 217
BanII G_rGCy'C
Cuts at: 1391 3089 1391
Size: 1698 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4799
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 1903
Size: 34 2024 34 3678
Fragments arranged by size:
3678 2024 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3662 4872 3662
Size: 1210 4560
BclI T'GATC_A
Cuts at: 1548 2454 1548
Size: 906 4864
BfrBI ATG'CAT
Cuts at: 3013 3013
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4381 136
Size: 107 122 71 631 605 2709 1525
Fragments arranged by size:
2709 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5765 5765
Size: 5770
BlpI GC'TnA_GC
Cuts at: 3187 3187
Size: 5770
Bme1580I G_kGCm'C
Cuts at: 1790 2627 3831 4987 1790
Size: 837 1204 1156 2573
Fragments arranged by size:
2573 1204 1156 837
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3501 4459 442
Size: 416 53 83 399 24 2084 958 1753
Fragments arranged by size:
2084 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2252 1056
Size: 1196 4574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 2450 3051 4430 1239
Size: 791 420 601 1379 2579
Fragments arranged by size:
2579 1379 791 601 420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3895 4760 5058 3895
Size: 865 298 4607
Fragments arranged by size:
4607 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4433 882
Size: 617 2934 2219
Fragments arranged by size:
2934 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 2967 5764 2967
Size: 2797 2973
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3577 3959 4594 275
Size: 53 83 186 936 2044 382 635 1451
Fragments arranged by size:
2044 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4203 4601 4875 5022 5433 4203
Size: 398 274 147 411 4540
Fragments arranged by size:
4540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5545
Fragments arranged by size:
5545 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2794 3048 1186
Size: 886 722 254 3908
Fragments arranged by size:
3908 886 722 254
BseYI C'CCAG_C
Cuts at: 2437 2798 3050 4973 5469 2437
Size: 361 252 1923 496 2738
Fragments arranged by size:
2738 1923 496 361 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5197
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2368 3220 3981 5009 1540
Size: 275 186 367 852 761 1028 2301
Fragments arranged by size:
2301 1028 852 761 367 275 186
BsiHKAI G_wGCw'C
Cuts at: 2417 3831 3916 4987 2417
Size: 1414 85 1071 3200
Fragments arranged by size:
3200 1414 1071 85
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2417 2627 3089 3831 3916 4987 1391
Size: 399 627 210 462 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 627 462 399 210 85
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 3659 5389 3659
Size: 1730 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2492 3125 844
Size: 1648 633 3489
Fragments arranged by size:
3489 1648 633
BsrBI CCG'CTC
Cuts at: 1394 1823 3657 1394
Size: 429 1834 3507
Fragments arranged by size:
3507 1834 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4265 4439 65
Size: 1183 3017 174 1396
Fragments arranged by size:
3017 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2513 4414 4601 1700
Size: 110 703 1901 187 2869
Fragments arranged by size:
2869 1901 703 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 2113 1184
Size: 929 4841
BstXI CCAn_nnnn'nTGG
Cuts at: 2110 2593 2110
Size: 483 5287
BstYI r'GATC_y
Cuts at: 1972 2685 3100 3467 3852 3869 5321 5407 5419
Size: 713 415 367 385 17 1452 86 12
Cuts at: 5419 5765 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 713 415 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2331 513
Size: 552 939 327 3952
Fragments arranged by size:
3952 939 552 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5031
Fragments arranged by size:
5031 683 56
BtsI GCAGTG_nn'
Cuts at: 3286 4080 4100 3286
Size: 794 20 4956
Fragments arranged by size:
4956 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2358 2376
Size: 54 1425 50 275 18 315 213 18
Cuts at: 2376 2928 3217 4106 5735 8
Size: 552 289 889 1629 43
Fragments arranged by size:
1629 1425 889 552 315 289 275 213
54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2198 3700 1353
Size: 737 108 1502 3423
Fragments arranged by size:
3423 1502 737 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2450 2579 3051 3833 4430 5217
Size: 393 398 420 129 472 782 597 787
Cuts at: 5217 5485 1239
Size: 268 1524
Fragments arranged by size:
1524 787 782 597 472 420 398 393
268 129
EcoNI CCTnn'n_nnAGG
Cuts at: 2941 2941
Size: 5770
EcoRI G'AATT_C
Cuts at: 2036 3079 3457 2036
Size: 1043 378 4349
Fragments arranged by size:
4349 1043 378
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 4917 1678
Size: 3239 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4411 4443 4485 4517 1373
Size: 32 135 32 2839 32 42 32 2626
Fragments arranged by size:
2839 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2212 3114 3360 669
Size: 988 281 274 902 246 3079
Fragments arranged by size:
3079 988 902 281 274 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4884 5068 1305
Size: 3579 184 2007
Fragments arranged by size:
3579 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 2360 2378 10
Size: 54 1425 343 315 213 18 3402
Fragments arranged by size:
3402 1425 343 315 213 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2303 2618 2744 2852 3008 3728
Size: 648 1011 126 315 126 108 156 720
Cuts at: 3728 4087 4246 518
Size: 359 159 2042
Fragments arranged by size:
2042 1011 720 648 359 315 159 156
126 126 108
MspA1I CmG'CkG
Cuts at: 1606 3186 3863 5011 5256 1606
Size: 1580 677 1148 245 2120
Fragments arranged by size:
2120 1580 1148 677 245
NaeI GCC'GGC
Cuts at: 2515 2515
Size: 5770
NcoI C'CATG_G
Cuts at: 513 1065 2004 2331 513
Size: 552 939 327 3952
Fragments arranged by size:
3952 939 552 327
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NgoMIV G'CCGG_C
Cuts at: 2513 2513
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 2248 1052
Size: 1196 4574
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NsiI A_TGCA'T
Cuts at: 3015 3015
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 3128 4673 1132
Size: 97 1899 1545 2229
Fragments arranged by size:
2229 1899 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2905 1348
Size: 1557 4213
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5770
PfoI T'CCnGG_A
Cuts at: 2977 2977
Size: 5770
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PstI C_TGCA'G
Cuts at: 830 3122 830
Size: 2292 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 4018 5730 4018
Size: 1712 4058
SexAI A'CCwGG_T
Cuts at: 2109 2109
Size: 5770
SfcI C'TryA_G
Cuts at: 826 1045 2872 3118 4253 4934 5125 826
Size: 219 1827 246 1135 681 191 1471
Fragments arranged by size:
1827 1471 1135 681 246 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 3097 1872
Size: 1225 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3874 4775 5037 820
Size: 197 926 1931 901 262 1553
Fragments arranged by size:
1931 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5770
StyI C'CwwG_G
Cuts at: 513 1065 2004 2331 2942 3209 513
Size: 552 939 327 611 267 3074
Fragments arranged by size:
3074 939 611 552 327 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2191 2315 3797 3814 3967 4152 4597
Size: 1255 611 124 1482 17 153 185 445
Cuts at: 4597 325
Size: 1498
Fragments arranged by size:
1498 1482 1255 611 445 185 153 124
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2778 4016
Size: 274 80 33 51 1443 204 597 1238
Cuts at: 4016 5728 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1238 597 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5770
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2284
Size: 5770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 3095 1870
Size: 1225 4545
XmnI GAAnn'nnTTC
Cuts at: 2714 3899 2714
Size: 1185 4585
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NarI NruI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI