Restriction map for
pFN21AE0886
(Circular) (Six-base) MAPSORT of: pFN21AE0886.seq Check: 1083 from: 1 to: 5674
pFN21AE0886 5674 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3017 1937
Size: 1080 4594
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2606 3737 5121 5389 1632
Size: 974 1131 1384 268 1917
Fragments arranged by size:
1917 1384 1131 974 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 4573 5550 1203
Size: 3370 977 1327
Fragments arranged by size:
3370 1327 977
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 2177 2870 1166
Size: 1011 693 3970
Fragments arranged by size:
3970 1011 693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AlwNI CAG_nnn'CTG
Cuts at: 3417 4989 3417
Size: 1572 4102
ApaLI G'TGCA_C
Cuts at: 3731 4887 3731
Size: 1156 4518
ApoI r'AATT_y
Cuts at: 1493 2983 3209 3361 5470 1493
Size: 1490 226 152 2109 1697
Fragments arranged by size:
2109 1697 1490 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2607 2999 1870
Size: 73 664 392 4545
Fragments arranged by size:
4545 664 392 73
BamHI G'GATC_C
Cuts at: 3004 3371 3004
Size: 367 5307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2995 4450 618
Size: 325 217 627 1208 1455 1842
Fragments arranged by size:
1842 1455 1208 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2444 2646 2932 2993 1391
Size: 1053 202 286 61 4072
Fragments arranged by size:
4072 1053 286 202 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4703
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3865 3899 1903
Size: 34 1928 34 3678
Fragments arranged by size:
3678 1928 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4285 136
Size: 107 122 71 631 605 2613 1525
Fragments arranged by size:
2613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 3091 3091
Size: 5674
Bme1580I G_kGCm'C
Cuts at: 1790 3735 4891 1790
Size: 1945 1156 2573
Fragments arranged by size:
2573 1945 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3405 4363 442
Size: 416 53 83 399 24 1988 958 1753
Fragments arranged by size:
1988 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4334 1239
Size: 3095 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2693 3799 4664 4962 2693
Size: 1106 865 298 3405
Fragments arranged by size:
3405 1106 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2807 4337 882
Size: 617 1308 1530 2219
Fragments arranged by size:
2219 1530 1308 617
BsaAI yAC'GTr
Cuts at: 493 1204 3465 493
Size: 711 2261 2702
Fragments arranged by size:
2702 2261 711
BsaBI GATnn'nnATC
Cuts at: 2630 5668 2630
Size: 3038 2636
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3481 3863 4498 275
Size: 53 83 186 936 1948 382 635 1451
Fragments arranged by size:
1948 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4107 4505 4779 4926 5337 4107
Size: 398 274 147 411 4444
Fragments arranged by size:
4444 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5449
Fragments arranged by size:
5449 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2689 1186
Size: 937 566 4171
Fragments arranged by size:
4171 937 566
BseYI C'CCAG_C
Cuts at: 4877 5373 4877
Size: 496 5178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2405 2559 1163
Size: 573 669 154 4278
Fragments arranged by size:
4278 669 573 154
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 3885 4913 1540
Size: 275 186 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3735 3820 4891 3735
Size: 85 1071 4518
Fragments arranged by size:
4518 1071 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2444 2646 2932 2993 3735 3820 4891
Size: 399 654 202 286 61 742 85 1071
Cuts at: 4891 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 654 399 286 202 85
61
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 2436 3563 5293 2436
Size: 1127 1730 2817
Fragments arranged by size:
2817 1730 1127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3029 844
Size: 2185 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3561 1394
Size: 429 1738 3507
Fragments arranged by size:
3507 1738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4169 4343 65
Size: 1183 2921 174 1396
Fragments arranged by size:
2921 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4318 4505 1700
Size: 110 2508 187 2869
Fragments arranged by size:
2869 2508 187 110
BsrGI T'GTAC_A
Cuts at: 96 2862 96
Size: 2766 2908
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 3734 4746 3734
Size: 1012 4662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2257 794
Size: 1463 4211
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 2872 1184
Size: 1688 3986
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2122
Size: 5674
BstYI r'GATC_y
Cuts at: 1972 2329 3004 3371 3756 3773 5225 5311 5323
Size: 357 675 367 385 17 1452 86 12
Cuts at: 5323 5669 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 675 385 367 357 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2906 2906
Size: 5674
BtgI C'CryG_G
Cuts at: 513 1065 2355 2460 513
Size: 552 1290 105 3727
Fragments arranged by size:
3727 1290 552 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4935
Fragments arranged by size:
4935 683 56
BtsI GCAGTG_nn'
Cuts at: 3190 3984 4004 3190
Size: 794 20 4860
Fragments arranged by size:
4860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2352 2376
Size: 54 1425 50 275 18 270 252 24
Cuts at: 2376 2520 3121 4010 5639 8
Size: 144 601 889 1629 43
Fragments arranged by size:
1629 1425 889 601 275 270 252 144
54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3604 1353
Size: 2251 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2606 3737 4334 5121 5389 1239
Size: 393 974 1131 597 787 268 1524
Fragments arranged by size:
1524 1131 974 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2679 2713 2679
Size: 34 5640
EcoRI G'AATT_C
Cuts at: 2983 3361 2983
Size: 378 5296
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 2471 4821 1678
Size: 793 2350 2531
Fragments arranged by size:
2531 2350 793
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4315 4347 4389 4421 1373
Size: 32 135 32 2743 32 42 32 2626
Fragments arranged by size:
2743 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3018 3264 669
Size: 988 281 1080 246 3079
Fragments arranged by size:
3079 1080 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5674
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2086 4788 4972 1305
Size: 781 2702 184 2007
Fragments arranged by size:
2702 2007 781 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2102 2354 2378 2522 10
Size: 54 1425 343 270 252 24 144 3162
Fragments arranged by size:
3162 1425 343 270 252 144 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2849 2855 2870 3632 3991 4150
Size: 648 1011 672 6 15 762 359 159
Cuts at: 4150 518
Size: 2042
Fragments arranged by size:
2042 1011 762 672 648 359 159 15
6
MspA1I CmG'CkG
Cuts at: 1606 3090 3767 4915 5160 1606
Size: 1484 677 1148 245 2120
Fragments arranged by size:
2120 1484 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2355 513
Size: 552 1290 3832
Fragments arranged by size:
3832 1290 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 2260 3032 4577 1132
Size: 97 1031 772 1545 2229
Fragments arranged by size:
2229 1545 1031 772 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5674
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2679 2713 2679
Size: 34 5640
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5674
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PspXI vC'TCGA_Gb
Cuts at: 2607 2607
Size: 5674
PstI C_TGCA'G
Cuts at: 830 2253 3026 830
Size: 1423 773 3478
Fragments arranged by size:
3478 1423 773
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2249 2899 3022 4157 4838 5029 826
Size: 219 1204 650 123 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 681 650 219 191 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2607 2708 3778 4679 4941 820
Size: 197 926 664 101 1070 901 262 1553
Fragments arranged by size:
1553 1070 926 901 664 262 197 101
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 3598 5
Size: 47 3546 2081
Fragments arranged by size:
3546 2081 47
StuI AGG'CCT
Cuts at: 1517 2741 1517
Size: 1224 4450
StyI C'CwwG_G
Cuts at: 513 1065 2355 2674 3113 513
Size: 552 1290 319 439 3074
Fragments arranged by size:
3074 1290 552 439 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2467 2611 3701 3718 3871 4056 4501
Size: 1255 887 144 1090 17 153 185 445
Cuts at: 4501 325
Size: 1498
Fragments arranged by size:
1498 1255 1090 887 445 185 153 144
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2307 2862 3920
Size: 274 80 33 51 1443 330 555 1058
Cuts at: 3920 5632 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1058 555 330 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 2846 1316
Size: 1530 4144
XbaI T'CTAG_A
Cuts at: 2332 3010 2332
Size: 678 4996
XhoI C'TCGA_G
Cuts at: 1943 2607 1943
Size: 664 5010
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 3803 3803
Size: 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI