Restriction map for
pFN21AE0875
(Circular) (Six-base) MAPSORT of: pFN21AE0875.seq Check: 25 from: 1 to: 5746
pFN21AE0875 5746 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3089 1937
Size: 1152 4594
Acc65I G'GTAC_C
Cuts at: 2852 3067 2852
Size: 215 5531
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3809 5193 5461 1632
Size: 2177 1384 268 1917
Fragments arranged by size:
2177 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 2690 1166
Size: 1524 4222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5746
AlwNI CAG_nnn'CTG
Cuts at: 3489 5061 3489
Size: 1572 4174
ApaLI G'TGCA_C
Cuts at: 3803 4959 3803
Size: 1156 4590
ApoI r'AATT_y
Cuts at: 1493 2126 2557 2779 3055 3281 3433 5542 1493
Size: 633 431 222 276 226 152 2109 1697
Fragments arranged by size:
2109 1697 633 431 276 226 222 152
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 3071 1870
Size: 73 1128 4545
Fragments arranged by size:
4545 1128 73
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2852 3067 4522 618
Size: 325 217 627 1065 215 1455 1842
Fragments arranged by size:
1842 1455 1065 627 325 217 215
BanII G_rGCy'C
Cuts at: 1391 3065 1391
Size: 1674 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2800 928
Size: 971 901 3874
Fragments arranged by size:
3874 971 901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3937 3971 1903
Size: 34 2000 34 3678
Fragments arranged by size:
3678 2000 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2144 3638 4848 2144
Size: 1494 1210 3042
Fragments arranged by size:
3042 1494 1210
BclI T'GATC_A
Cuts at: 1548 2838 1548
Size: 1290 4456
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4357 136
Size: 107 122 71 631 605 2685 1525
Fragments arranged by size:
2685 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2063 5741 2063
Size: 3678 2068
BlpI GC'TnA_GC
Cuts at: 2818 3163 2818
Size: 345 5401
Bme1580I G_kGCm'C
Cuts at: 1790 3807 4963 1790
Size: 2017 1156 2573
Fragments arranged by size:
2573 2017 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4435 442
Size: 416 53 83 399 24 2060 958 1753
Fragments arranged by size:
2060 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4406 1239
Size: 3167 2579
Bpu10I CC'TnA_GC
Cuts at: 2501 2501
Size: 5746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2633 2871 3007 3871 4736 5034 2633
Size: 238 136 864 865 298 3345
Fragments arranged by size:
3345 865 864 298 238 136
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4409 882
Size: 617 2910 2219
Fragments arranged by size:
2910 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2814 3537 493
Size: 711 1610 723 2702
Fragments arranged by size:
2702 1610 723 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3553 3935 4570 275
Size: 53 83 186 936 2020 382 635 1451
Fragments arranged by size:
2020 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2546 4179 4577 4851 4998 5409 2546
Size: 1633 398 274 147 411 2883
Fragments arranged by size:
2883 1633 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5521
Fragments arranged by size:
5521 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2311 1186
Size: 1125 4621
BseYI C'CCAG_C
Cuts at: 4949 5445 4949
Size: 496 5250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2406 2411 1163
Size: 573 670 5 4498
Fragments arranged by size:
4498 670 573 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2819 3807 3892 4963 2819
Size: 988 85 1071 3602
Fragments arranged by size:
3602 1071 988 85
BsmI GAATG_Cn'
Cuts at: 3257 3350 3257
Size: 93 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2819 3065 3807 3892 4963 1391
Size: 399 1029 246 742 85 1071 2174
Fragments arranged by size:
2174 1071 1029 742 399 246 85
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3101 844
Size: 2257 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2224 2418 4241 4415 65
Size: 1183 976 194 1823 174 1396
Fragments arranged by size:
1823 1396 1183 976 194 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4390 4577 1700
Size: 110 2580 187 2869
Fragments arranged by size:
2869 2580 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5746
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5746
BstYI r'GATC_y
Cuts at: 1972 2063 3076 3443 3828 3845 5297 5383 5395
Size: 91 1013 367 385 17 1452 86 12
Cuts at: 5395 5741 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1013 385 367 346 91 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2688 513
Size: 552 1623 3571
Fragments arranged by size:
3571 1623 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 2394 3262 4056 4076 2394
Size: 868 794 20 4064
Fragments arranged by size:
4064 868 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2119 4753 809
Size: 507 803 2634 1802
Fragments arranged by size:
2634 1802 803 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2466 3193 4082
Size: 54 1425 50 275 18 636 727 889
Cuts at: 4082 5711 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 727 636 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3676 1353
Size: 2323 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3809 4406 5193 5461 1239
Size: 393 2177 597 787 268 1524
Fragments arranged by size:
2177 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2504 2504
Size: 5746
EcoRI G'AATT_C
Cuts at: 2779 3055 3433 2779
Size: 276 378 5092
Fragments arranged by size:
5092 378 276
EcoRV GAT'ATC
Cuts at: 2305 2697 2305
Size: 392 5354
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 4893 1678
Size: 3215 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2478 2510 4387 4419 4461
Size: 32 135 32 906 32 1877 32 42
Cuts at: 4461 4493 1373
Size: 32 2626
Fragments arranged by size:
2626 1877 906 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2421 3090 3336 669
Size: 988 281 483 669 246 3079
Fragments arranged by size:
3079 988 669 483 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 2856 3071 2856
Size: 215 5531
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4860 5044 1305
Size: 3555 184 2007
Fragments arranged by size:
3555 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2468 10
Size: 54 1425 343 636 3288
Fragments arranged by size:
3288 1425 636 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 2690 3704 4063 4222 518
Size: 648 1101 423 1014 359 159 2042
Fragments arranged by size:
2042 1101 1014 648 423 359 159
MspA1I CmG'CkG
Cuts at: 1606 2564 3162 3839 4987 5232 1606
Size: 958 598 677 1148 245 2120
Fragments arranged by size:
2120 1148 958 677 598 245
NcoI C'CATG_G
Cuts at: 513 1065 2688 513
Size: 552 1623 3571
Fragments arranged by size:
3571 1623 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 2631 3104 4649 1132
Size: 97 1402 473 1545 2229
Fragments arranged by size:
2229 1545 1402 473 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5746
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5746
PfoI T'CCnGG_A
Cuts at: 2298 2298
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PmlI CAC'GTG
Cuts at: 2814 2814
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PstI C_TGCA'G
Cuts at: 830 3098 830
Size: 2268 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 2564 2564
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 2980 3094 4229 4910 5101 826
Size: 219 1935 114 1135 681 191 1471
Fragments arranged by size:
1935 1471 1135 681 219 191 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 3073 1872
Size: 1201 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2648 2886 2986 3850 4751 5013
Size: 197 926 705 238 100 864 901 262
Cuts at: 5013 820
Size: 1553
Fragments arranged by size:
1553 926 901 864 705 262 238 197
100
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 2611 2637 2680 3020 3670 5
Size: 47 2559 26 43 340 650 2081
Fragments arranged by size:
2559 2081 650 340 47 43 26
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5746
StyI C'CwwG_G
Cuts at: 513 1065 2688 3185 513
Size: 552 1623 497 3074
Fragments arranged by size:
3074 1623 552 497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2087 2974 3773 3790 3943 4128 4573
Size: 1255 507 887 799 17 153 185 445
Cuts at: 4573 325
Size: 1498
Fragments arranged by size:
1498 1255 887 799 507 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2024 2271 3992
Size: 274 80 33 51 1443 47 247 1721
Cuts at: 3992 5704 96
Size: 1712 138
Fragments arranged by size:
1721 1712 1443 274 247 138 80 51
47 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2373 2405 5706 5738 2373
Size: 32 3301 32 2381
Fragments arranged by size:
3301 2381 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 3071 1870
Size: 1201 4545
XmnI GAAnn'nnTTC
Cuts at: 2130 2368 2557 3875 2130
Size: 238 189 1318 4001
Fragments arranged by size:
4001 1318 238 189
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI