Restriction map for
pFN21AE0873
(Circular) (Six-base) MAPSORT of: pFN21AE0873.seq Check: 436 from: 1 to: 5671
pFN21AE0873 5671 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3734 5118 5386 1632
Size: 2102 1384 268 1917
Fragments arranged by size:
2102 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 2184 2223 2723 4570 5547 1203
Size: 981 39 500 1847 977 1327
Fragments arranged by size:
1847 1327 981 977 500 39
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 2147 2234 2591 1166
Size: 981 87 357 4246
Fragments arranged by size:
4246 981 357 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5671
AlwNI CAG_nnn'CTG
Cuts at: 2080 2161 3414 4986 2080
Size: 81 1253 1572 2765
Fragments arranged by size:
2765 1572 1253 81
ApaI G_GGCC'C
Cuts at: 2517 2655 2517
Size: 138 5533
ApaLI G'TGCA_C
Cuts at: 2452 3728 4884 2452
Size: 1276 1156 3239
Fragments arranged by size:
3239 1276 1156
ApoI r'AATT_y
Cuts at: 1493 2980 3206 3358 5467 1493
Size: 1487 226 152 2109 1697
Fragments arranged by size:
2109 1697 1487 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2996 1870
Size: 73 1053 4545
Fragments arranged by size:
4545 1053 73
BamHI G'GATC_C
Cuts at: 3001 3368 3001
Size: 367 5304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2992 4447 618
Size: 325 217 627 1205 1455 1842
Fragments arranged by size:
1842 1455 1205 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2424 2517 2655 2990 1391
Size: 622 411 93 138 335 4072
Fragments arranged by size:
4072 622 411 335 138 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2824 928
Size: 971 925 3775
Fragments arranged by size:
3775 971 925
BbvCI CC'TCA_GC
Cuts at: 2324 2324
Size: 5671
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 1903
Size: 34 1925 34 3678
Fragments arranged by size:
3678 1925 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5671
BfrBI ATG'CAT
Cuts at: 2413 2413
Size: 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2297 2357 4282
Size: 107 122 71 631 605 625 60 1925
Cuts at: 4282 136
Size: 1525
Fragments arranged by size:
1925 1525 631 625 605 122 107 71
60
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 3088 3088
Size: 5671
Bme1580I G_kGCm'C
Cuts at: 1790 2241 2456 2517 2655 3732 4888 1790
Size: 451 215 61 138 1077 1156 2573
Fragments arranged by size:
2573 1156 1077 451 215 138 61
BmgBI CAC'GTC
Cuts at: 1318 2226 1318
Size: 908 4763
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2657 2784 2858
Size: 416 53 83 399 24 1240 127 74
Cuts at: 2858 3402 4360 442
Size: 544 958 1753
Fragments arranged by size:
1753 1240 958 544 416 399 127 83
74 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2177 2231 4331 1239
Size: 938 54 2100 2579
Fragments arranged by size:
2579 2100 938 54
Bpu10I CC'TnA_GC
Cuts at: 2324 2324
Size: 5671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2899 3796 4661 4959 2899
Size: 897 865 298 3611
Fragments arranged by size:
3611 897 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2449 4334 882
Size: 617 950 1885 2219
Fragments arranged by size:
2219 1885 950 617
BsaAI yAC'GTr
Cuts at: 493 1204 3462 493
Size: 711 2258 2702
Fragments arranged by size:
2702 2258 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 2935 2965 1178
Size: 30 165 30 801 30 701 30 3884
Fragments arranged by size:
3884 801 701 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5671
BseYI C'CCAG_C
Cuts at: 4874 5370 4874
Size: 496 5175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 1163
Size: 573 417 4681
Fragments arranged by size:
4681 573 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3882 4910 1540
Size: 275 186 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2456 3732 3817 4888 2456
Size: 1276 85 1071 3239
Fragments arranged by size:
3239 1276 1071 85
BsmI GAATG_Cn'
Cuts at: 3182 3275 3182
Size: 93 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2241 2424 2456 2517 2655 2990
Size: 399 223 228 183 32 61 138 335
Cuts at: 2990 3732 3817 4888 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 335 228 223 183
138 85 61 32
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 2916 3560 5290 2916
Size: 644 1730 3297
Fragments arranged by size:
3297 1730 644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2265 3026 844
Size: 1421 761 3489
Fragments arranged by size:
3489 1421 761
BsrBI CCG'CTC
Cuts at: 1394 1823 3558 1394
Size: 429 1735 3507
Fragments arranged by size:
3507 1735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4166 4340 65
Size: 1183 2918 174 1396
Fragments arranged by size:
2918 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4315 4502 1700
Size: 110 2505 187 2869
Fragments arranged by size:
2869 2505 187 110
BsrGI T'GTAC_A
Cuts at: 96 2181 96
Size: 2085 3586
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2080 794
Size: 1286 4385
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 2593 1184
Size: 1409 4262
BstXI CCAn_nnnn'nTGG
Cuts at: 2437 2437
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 3001 3368 3753 3770 5222 5308 5320 5666
Size: 1029 367 385 17 1452 86 12 346
Cuts at: 5666 1972
Size: 1977
Fragments arranged by size:
1977 1452 1029 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2145 513
Size: 552 939 141 4039
Fragments arranged by size:
4039 939 552 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 2852 3187 3981 4001 2852
Size: 335 794 20 4522
Fragments arranged by size:
4522 794 335 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DraIII CAC_nnn'GTG
Cuts at: 2337 2337
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 2701 3118
Size: 54 1425 50 275 18 546 325 417
Cuts at: 3118 4007 5636 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 546 417 325 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3601 1353
Size: 2248 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4258 4632 4778 387
Size: 1064 138 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 2231 3734 4331 5118 5386 1239
Size: 393 545 54 1503 597 787 268 1524
Fragments arranged by size:
1524 1503 787 597 545 393 268 54
EcoO109I rG'GnC_Cy
Cuts at: 2077 2158 2651 2652 2077
Size: 81 493 1 5096
Fragments arranged by size:
5096 493 81 1
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 2331 4818 1678
Size: 653 2487 2531
Fragments arranged by size:
2531 2487 653
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2568 2600 4312 4344 4386
Size: 32 135 32 996 32 1712 32 42
Cuts at: 4386 4418 1373
Size: 32 2626
Fragments arranged by size:
2626 1712 996 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3261 669
Size: 988 281 1077 246 3079
Fragments arranged by size:
3079 1077 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 2613 3270 2613
Size: 657 5014
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2481 4785 4969 1305
Size: 1176 2304 184 2007
Fragments arranged by size:
2304 2007 1176 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3303
Fragments arranged by size:
3303 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2147 2234 2591 2780 3629 3988
Size: 648 978 3 87 357 189 849 359
Cuts at: 3988 4147 518
Size: 159 2042
Fragments arranged by size:
2042 978 849 648 359 357 189 159
87 3
MspA1I CmG'CkG
Cuts at: 1606 2044 3087 3764 4912 5157 1606
Size: 438 1043 677 1148 245 2120
Fragments arranged by size:
2120 1148 1043 677 438 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4180
Fragments arranged by size:
4180 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NsiI A_TGCA'T
Cuts at: 2415 2415
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 2188 2727 3029 4574 1132
Size: 97 959 539 302 1545 2229
Fragments arranged by size:
2229 1545 959 539 302 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5671
PciI A'CATG_T
Cuts at: 2184 2723 4570 2184
Size: 539 1847 3285
Fragments arranged by size:
3285 1847 539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PspOMI G'GGCC_C
Cuts at: 2513 2651 2513
Size: 138 5533
PstI C_TGCA'G
Cuts at: 830 2387 3023 830
Size: 1557 636 3478
Fragments arranged by size:
3478 1557 636
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
PvuII CAG'CTG
Cuts at: 2044 2044
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SexAI A'CCwGG_T
Cuts at: 2429 2429
Size: 5671
SfcI C'TryA_G
Cuts at: 826 1045 2383 2872 3019 4154 4835 5026 826
Size: 219 1338 489 147 1135 681 191 1471
Fragments arranged by size:
1471 1338 1135 681 489 219 191 147
SfiI GGCCn_nnn'nGGCC
Cuts at: 2357 2357
Size: 5671
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2878 3775 4676 4938 820
Size: 197 926 935 897 901 262 1553
Fragments arranged by size:
1553 935 926 901 897 262 197
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5671
StyI C'CwwG_G
Cuts at: 513 1065 2004 3110 513
Size: 552 939 1106 3074
Fragments arranged by size:
3074 1106 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3698 3715 3868 4053 4498 325
Size: 1255 2118 17 153 185 445 1498
Fragments arranged by size:
2118 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2945 3917
Size: 274 80 33 51 1443 204 764 972
Cuts at: 3917 5629 96
Size: 1712 138
Fragments arranged by size:
1712 1443 972 764 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5631 5663 5631
Size: 32 5639
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2437 2437
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 2689 3800 2689
Size: 1111 4560
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfoI SrfI SwaI