Restriction map for
pFN21AE0864
(Circular) (Six-base) MAPSORT of: pFN21AE0864.seq Check: 9175 from: 1 to: 5662
pFN21AE0864 5662 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2747 2747
Size: 5662
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3472 278
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2850 3005 1937
Size: 913 155 4594
Fragments arranged by size:
4594 913 155
Acc65I G'GTAC_C
Cuts at: 2983 2983
Size: 5662
AclI AA'CG_TT
Cuts at: 1563 2961 3789 4162 1563
Size: 1398 828 373 3063
Fragments arranged by size:
3063 1398 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3725 5109 5377 1632
Size: 2093 1384 268 1917
Fragments arranged by size:
2093 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5465
AflIII A'CryG_T
Cuts at: 1203 2673 4561 5538 1203
Size: 1470 1888 977 1327
Fragments arranged by size:
1888 1470 1327 977
AgeI A'CCGG_T
Cuts at: 4493 4493
Size: 5662
AhdI GACnn_n'nnGTC
Cuts at: 4391 4391
Size: 5662
AleI CACnn'nnGTG
Cuts at: 1166 2240 1166
Size: 1074 4588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2462 2651 1184
Size: 1278 189 4195
Fragments arranged by size:
4195 1278 189
AlwNI CAG_nnn'CTG
Cuts at: 2078 2449 2951 3405 4977 2078
Size: 371 502 454 1572 2763
Fragments arranged by size:
2763 1572 502 454 371
ApaI G_GGCC'C
Cuts at: 2281 2314 2461 2281
Size: 33 147 5482
Fragments arranged by size:
5482 147 33
ApaLI G'TGCA_C
Cuts at: 2164 2437 2600 3719 4875 2164
Size: 273 163 1119 1156 2951
Fragments arranged by size:
2951 1156 1119 273 163
ApoI r'AATT_y
Cuts at: 1493 2081 2971 3197 3349 5458 1493
Size: 588 890 226 152 2109 1697
Fragments arranged by size:
2109 1697 890 588 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4216 160
Size: 872 3184 1606
Fragments arranged by size:
3184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2987 1870
Size: 73 1044 4545
Fragments arranged by size:
4545 1044 73
BamHI G'GATC_C
Cuts at: 2992 3359 2992
Size: 367 5295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2983 4438 618
Size: 325 217 627 1196 1455 1842
Fragments arranged by size:
1842 1455 1196 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2281 2314 2461 2981 1391
Size: 890 33 147 520 4072
Fragments arranged by size:
4072 890 520 147 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2017 928
Size: 971 118 4573
Fragments arranged by size:
4573 971 118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3853 3887 1903
Size: 34 50 34 1832 34 3678
Fragments arranged by size:
3678 1832 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3554 4764 3554
Size: 1210 4452
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5662
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4273 136
Size: 107 122 71 631 605 2601 1525
Fragments arranged by size:
2601 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5657 5657
Size: 5662
BlpI GC'TnA_GC
Cuts at: 3079 3079
Size: 5662
Bme1580I G_kGCm'C
Cuts at: 1790 2168 2281 2314 2441 2461 2604 3723 4879
Size: 378 113 33 127 20 143 1119 1156
Cuts at: 4879 1790
Size: 2573
Fragments arranged by size:
2573 1156 1119 378 143 127 113 33
20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3393 4351 442
Size: 416 53 83 399 24 1976 958 1753
Fragments arranged by size:
1976 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4322 1239
Size: 3083 2579
Bpu10I CC'TnA_GC
Cuts at: 2696 2696
Size: 5662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3787 4652 4950 3787
Size: 865 298 4499
Fragments arranged by size:
4499 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4325 882
Size: 617 2826 2219
Fragments arranged by size:
2826 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2674 3453 493
Size: 711 1470 779 2702
Fragments arranged by size:
2702 1470 779 711
BsaBI GATnn'nnATC
Cuts at: 5656 5656
Size: 5662
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2955 3469 3851 4486
Size: 53 83 186 936 1422 514 382 635
Cuts at: 4486 275
Size: 1451
Fragments arranged by size:
1451 1422 936 635 514 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4095 4493 4767 4914 5325 4095
Size: 398 274 147 411 4432
Fragments arranged by size:
4432 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5437
Fragments arranged by size:
5437 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2105 2938 1186
Size: 916 3 833 3910
Fragments arranged by size:
3910 916 833 3
BseYI C'CCAG_C
Cuts at: 2449 4865 5361 2449
Size: 2416 496 2750
Fragments arranged by size:
2750 2416 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2390 1163
Size: 573 654 4435
Fragments arranged by size:
4435 654 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3873 4901 1540
Size: 275 186 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2168 2441 2604 2920 3723 3808 4879 2168
Size: 273 163 316 803 85 1071 2951
Fragments arranged by size:
2951 1071 803 316 273 163 85
BsmI GAATG_Cn'
Cuts at: 3173 3266 3173
Size: 93 5569
BsmBI CGTCTCn'nnnn_
Cuts at: 2671 2671
Size: 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2168 2281 2314 2441 2461 2604 2920
Size: 399 378 113 33 127 20 143 316
Cuts at: 2920 2981 3723 3808 4879 1391
Size: 61 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 378 316 143 127
113 85 61 33 20
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 2421 3551 5281 2421
Size: 1130 1730 2802
Fragments arranged by size:
2802 1730 1130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2747 2805 3017 844
Size: 1903 58 212 3489
Fragments arranged by size:
3489 1903 212 58
BsrBI CCG'CTC
Cuts at: 1394 1823 3549 1394
Size: 429 1726 3507
Fragments arranged by size:
3507 1726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4157 4331 65
Size: 1183 2909 174 1396
Fragments arranged by size:
2909 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4306 4493 1700
Size: 110 2496 187 2869
Fragments arranged by size:
2869 2496 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5662
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 2633 2789 3722 4734 2633
Size: 156 933 1012 3561
Fragments arranged by size:
3561 1012 933 156
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5662
BstBI TT'CG_AA
Cuts at: 4466 4466
Size: 5662
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5662
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2272 2296 2011
Size: 261 24 5377
Fragments arranged by size:
5377 261 24
BstYI r'GATC_y
Cuts at: 1972 2992 3359 3744 3761 5213 5299 5311 5657
Size: 1020 367 385 17 1452 86 12 346
Cuts at: 5657 1972
Size: 1977
Fragments arranged by size:
1977 1452 1020 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2894 2894
Size: 5662
BtgI C'CryG_G
Cuts at: 513 1065 2241 2757 513
Size: 552 1176 516 3418
Fragments arranged by size:
3418 1176 552 516
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2571 487
Size: 683 56 1345 3578
Fragments arranged by size:
3578 1345 683 56
BtsI GCAGTG_nn'
Cuts at: 3178 3972 3992 3178
Size: 794 20 4848
Fragments arranged by size:
4848 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2032 2067 529
Size: 35 1468 35 4124
Fragments arranged by size:
4124 1468 35 35
DraI TTT'AAA
Cuts at: 2966 2966
Size: 5662
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4669 809
Size: 507 3353 1802
Fragments arranged by size:
3353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2524 3109 3998
Size: 54 1425 50 275 18 694 585 889
Cuts at: 3998 5627 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 694 585 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 1537
Size: 275 1297 4090
Fragments arranged by size:
4090 1297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 2111 2480 3592 1353
Size: 749 9 369 1112 3423
Fragments arranged by size:
3423 1112 749 369 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4249 4623 4769 387
Size: 1064 138 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3725 4322 5109 5377 1239
Size: 393 2093 597 787 268 1524
Fragments arranged by size:
2093 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2277 2310 2457 2458 2277
Size: 33 147 1 5481
Fragments arranged by size:
5481 147 33 1
EcoRI G'AATT_C
Cuts at: 2081 2971 3349 2081
Size: 890 378 4394
Fragments arranged by size:
4394 890 378
FspI TGC'GCA
Cuts at: 4168 4168
Size: 5662
HaeII r_GCGC'y
Cuts at: 1678 4809 1678
Size: 3131 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4303 4335 4377 4409 1373
Size: 32 135 32 2731 32 42 32 2626
Fragments arranged by size:
2731 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3006 3252 669
Size: 988 281 1068 246 3079
Fragments arranged by size:
3079 1068 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5662
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KpnI G_GTAC'C
Cuts at: 2987 2987
Size: 5662
MfeI C'AATT_G
Cuts at: 3261 3261
Size: 5662
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2232 2289 4776 4960 1305
Size: 927 57 2487 184 2007
Fragments arranged by size:
2487 2007 927 184 57
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3840
Fragments arranged by size:
3840 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2156 2189 2240 2444 2597 2633
Size: 648 843 147 33 51 204 153 36
Cuts at: 2633 3620 3979 4138 518
Size: 987 359 159 2042
Fragments arranged by size:
2042 987 843 648 359 204 159 153
147 51 36 33
MspA1I CmG'CkG
Cuts at: 1606 2404 3078 3755 4903 5148 1606
Size: 798 674 677 1148 245 2120
Fragments arranged by size:
2120 1148 798 677 674 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5110
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5662
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5662
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 3020 4565 1132
Size: 97 1791 1545 2229
Fragments arranged by size:
2229 1791 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2461 1348
Size: 1113 4549
PciI A'CATG_T
Cuts at: 4561 4561
Size: 5662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2154 1108
Size: 1046 4616
PfoI T'CCnGG_A
Cuts at: 2220 2220
Size: 5662
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2039 2071 2689 2721 3731 3763 5244 5276 2039
Size: 32 618 32 1010 32 1481 32 2425
Fragments arranged by size:
2425 1481 1010 618 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5662
PsiI TTA'TAA
Cuts at: 3232 3232
Size: 5662
PspOMI G'GGCC_C
Cuts at: 2277 2310 2457 2277
Size: 33 147 5482
Fragments arranged by size:
5482 147 33
PstI C_TGCA'G
Cuts at: 830 2490 2772 2786 3014 830
Size: 1660 282 14 228 3478
Fragments arranged by size:
3478 1660 282 228 14
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SbfI CC_TGCA'GG
Cuts at: 3014 3014
Size: 5662
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SfcI C'TryA_G
Cuts at: 826 1045 2486 2768 2782 3010 4145 4826 5017
Size: 219 1441 282 14 228 1135 681 191
Cuts at: 5017 826
Size: 1471
Fragments arranged by size:
1471 1441 1135 681 282 228 219 191
14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2989 1872
Size: 1117 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3766 4667 4929 820
Size: 197 926 1823 901 262 1553
Fragments arranged by size:
1823 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3453 493
Size: 2960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 3020 1132
Size: 1888 3774
SspI AAT'ATT
Cuts at: 5 52 3586 5
Size: 47 3534 2081
Fragments arranged by size:
3534 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5662
StyI C'CwwG_G
Cuts at: 513 1065 3101 513
Size: 552 2036 3074
Fragments arranged by size:
3074 2036 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2146 2441 2796 3689 3706 3859 4044
Size: 1255 566 295 355 893 17 153 185
Cuts at: 4044 4489 325
Size: 445 1498
Fragments arranged by size:
1498 1255 893 566 445 355 295 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3908 5620 96
Size: 274 80 33 51 1443 1931 1712 138
Fragments arranged by size:
1931 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5622 5654 5622
Size: 32 5630
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5662
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2155 2155
Size: 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5662
XmaI C'CCGG_G
Cuts at: 1870 2987 1870
Size: 1117 4545
XmnI GAAnn'nnTTC
Cuts at: 3791 3791
Size: 5662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3470 276
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI