Restriction map for
pFN21AE0851
(Circular) (Six-base) MAPSORT of: pFN21AE0851.seq Check: 1930 from: 1 to: 5608
pFN21AE0851 5608 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2705 2705
Size: 5608
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 1937
Size: 1014 4594
Acc65I G'GTAC_C
Cuts at: 2929 2929
Size: 5608
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3671 5055 5323 1632
Size: 2039 1384 268 1917
Fragments arranged by size:
2039 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 2278 1166
Size: 1112 4496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5608
AlwNI CAG_nnn'CTG
Cuts at: 3351 4923 3351
Size: 1572 4036
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2009 2127 2463 2917 3143 3295 5404 1493
Size: 516 118 336 454 226 152 2109 1697
Fragments arranged by size:
2109 1697 516 454 336 226 152 118
AseI AT'TA_AT
Cuts at: 160 1032 2499 4162 160
Size: 872 1467 1663 1606
Fragments arranged by size:
1663 1606 1467 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2933 1870
Size: 73 990 4545
Fragments arranged by size:
4545 990 73
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2602 2929 4384 618
Size: 325 217 627 815 327 1455 1842
Fragments arranged by size:
1842 1455 815 627 327 325 217
BanII G_rGCy'C
Cuts at: 1391 2927 1391
Size: 1536 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3799 3833 1903
Size: 34 1862 34 3678
Fragments arranged by size:
3678 1862 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2382 3500 4710 2382
Size: 1118 1210 3280
Fragments arranged by size:
3280 1210 1118
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4219 136
Size: 107 122 71 631 605 2547 1525
Fragments arranged by size:
2547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5603 5603
Size: 5608
BlpI GC'TnA_GC
Cuts at: 3025 3025
Size: 5608
Bme1580I G_kGCm'C
Cuts at: 1790 3669 4825 1790
Size: 1879 1156 2573
Fragments arranged by size:
2573 1879 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3339 4297 442
Size: 416 53 83 399 24 1922 958 1753
Fragments arranged by size:
1922 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 4268 1239
Size: 887 2142 2579
Fragments arranged by size:
2579 2142 887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2516 3733 4598 4896 2516
Size: 1217 865 298 3228
Fragments arranged by size:
3228 1217 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 882
Size: 617 2772 2219
Fragments arranged by size:
2772 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2347 3399 493
Size: 711 1143 1052 2702
Fragments arranged by size:
2702 1143 1052 711
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3415 3797 4432 275
Size: 53 83 186 936 1882 382 635 1451
Fragments arranged by size:
1882 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4041 4439 4713 4860 5271 4041
Size: 398 274 147 411 4378
Fragments arranged by size:
4378 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5383
Fragments arranged by size:
5383 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5608
BseYI C'CCAG_C
Cuts at: 4811 5307 4811
Size: 496 5112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5035
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3058 3819 4847 1540
Size: 275 186 1057 761 1028 2301
Fragments arranged by size:
2301 1057 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3669 3754 4825 3669
Size: 85 1071 4452
Fragments arranged by size:
4452 1071 85
BsmI GAATG_Cn'
Cuts at: 2223 2400 3119 3212 2223
Size: 177 719 93 4619
Fragments arranged by size:
4619 719 177 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2927 3669 3754 4825 1391
Size: 399 1137 742 85 1071 2174
Fragments arranged by size:
2174 1137 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5271 5271
Size: 5608
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2705 2729 2963 844
Size: 1861 24 234 3489
Fragments arranged by size:
3489 1861 234 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2138 3495 1394
Size: 429 315 1357 3507
Fragments arranged by size:
3507 1357 429 315
BsrDI GCAATG_nn'
Cuts at: 65 1248 2107 4103 4277 65
Size: 1183 859 1996 174 1396
Fragments arranged by size:
1996 1396 1183 859 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4252 4439 1700
Size: 110 2442 187 2869
Fragments arranged by size:
2869 2442 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 3668 4680 3668
Size: 1012 4596
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5608
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2938 3305 3690 3707 5159 5245 5257 5603
Size: 966 367 385 17 1452 86 12 346
Cuts at: 5603 1972
Size: 1977
Fragments arranged by size:
1977 1452 966 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2852 2852
Size: 5608
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4117
Fragments arranged by size:
4117 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4869
Fragments arranged by size:
4869 683 56
BtsI GCAGTG_nn'
Cuts at: 3124 3918 3938 3124
Size: 794 20 4794
Fragments arranged by size:
4794 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5573
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DraIII CAC_nnn'GTG
Cuts at: 2310 2310
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3055 3944 5573
Size: 54 1425 50 275 18 1225 889 1629
Cuts at: 5573 8
Size: 43
Fragments arranged by size:
1629 1425 1225 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3538 1353
Size: 2185 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 3671 4268 5055 5323 1239
Size: 393 494 1545 597 787 268 1524
Fragments arranged by size:
1545 1524 787 597 494 393 268
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
EcoRV GAT'ATC
Cuts at: 2866 2866
Size: 5608
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 4755 1678
Size: 3077 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4249 4281 4323 4355 1373
Size: 32 135 32 2677 32 42 32 2626
Fragments arranged by size:
2677 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 3198 669
Size: 988 281 1014 246 3079
Fragments arranged by size:
3079 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2534 748
Size: 1786 3822
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KpnI G_GTAC'C
Cuts at: 2933 2933
Size: 5608
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2670 2849 4722 4906 1305
Size: 1365 179 1873 184 2007
Fragments arranged by size:
2007 1873 1365 184 179
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3786
Fragments arranged by size:
3786 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2278 2555 3566 3925 4084 518
Size: 648 1112 277 1011 359 159 2042
Fragments arranged by size:
2042 1112 1011 648 359 277 159
MspA1I CmG'CkG
Cuts at: 1606 2407 3024 3701 4849 5094 1606
Size: 801 617 677 1148 245 2120
Fragments arranged by size:
2120 1148 801 677 617 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4117
Fragments arranged by size:
4117 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5608
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2460 2966 4511 1132
Size: 97 1231 506 1545 2229
Fragments arranged by size:
2229 1545 1231 506 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5608
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5608
PfoI T'CCnGG_A
Cuts at: 2420 2420
Size: 5608
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PstI C_TGCA'G
Cuts at: 830 2960 830
Size: 2130 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
PvuII CAG'CTG
Cuts at: 2407 2407
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 3856 5568 3856
Size: 1712 3896
SfcI C'TryA_G
Cuts at: 826 1045 2439 2956 4091 4772 4963 826
Size: 219 1394 517 1135 681 191 1471
Fragments arranged by size:
1471 1394 1135 681 517 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2935 1872
Size: 1063 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2531 3712 4613 4875 820
Size: 197 926 588 1181 901 262 1553
Fragments arranged by size:
1553 1181 926 901 588 262 197
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 2251 3532 5
Size: 47 2199 1281 2081
Fragments arranged by size:
2199 2081 1281 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 2004 3047 513
Size: 552 939 1043 3074
Fragments arranged by size:
3074 1043 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3635 3652 3805 3990 4435 325
Size: 1255 2055 17 153 185 445 1498
Fragments arranged by size:
2055 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3854 5566 96
Size: 274 80 33 51 1443 1877 1712 138
Fragments arranged by size:
1877 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5568 5600 5568
Size: 32 5576
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2933 1870
Size: 1063 4545
XmnI GAAnn'nnTTC
Cuts at: 2013 2251 3737 2013
Size: 238 1486 3884
Fragments arranged by size:
3884 1486 238
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI