Restriction map for
pFN21AE0840
(Circular) (Six-base) MAPSORT of: pFN21AE0840.seq Check: 382 from: 1 to: 5740
pFN21AE0840 5740 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2339 2339
Size: 5740
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2251 3803 5187 5455 1632
Size: 619 1552 1384 268 1917
Fragments arranged by size:
1917 1552 1384 619 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AlwNI CAG_nnn'CTG
Cuts at: 3483 5055 3483
Size: 1572 4168
ApaLI G'TGCA_C
Cuts at: 3797 4953 3797
Size: 1156 4584
ApoI r'AATT_y
Cuts at: 1493 2454 3019 3049 3275 3427 5536 1493
Size: 961 565 30 226 152 2109 1697
Fragments arranged by size:
2109 1697 961 565 226 152 30
AseI AT'TA_AT
Cuts at: 160 1032 2840 4294 160
Size: 872 1808 1454 1606
Fragments arranged by size:
1808 1606 1454 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 3065 1870
Size: 73 154 968 4545
Fragments arranged by size:
4545 968 154 73
BamHI G'GATC_C
Cuts at: 2093 2475 3070 3437 2093
Size: 382 595 367 4396
Fragments arranged by size:
4396 595 382 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3061 4516 618
Size: 325 217 627 1274 1455 1842
Fragments arranged by size:
1842 1455 1274 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3059 1391
Size: 1668 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3931 3965 1903
Size: 34 1994 34 3678
Fragments arranged by size:
3678 1994 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3632 4842 3632
Size: 1210 4530
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2225 2750 4351
Size: 107 122 71 631 605 553 525 1601
Cuts at: 4351 136
Size: 1525
Fragments arranged by size:
1601 1525 631 605 553 525 122 107
71
BglII A'GATC_T
Cuts at: 5735 5735
Size: 5740
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 3801 4957 1790
Size: 2011 1156 2573
Fragments arranged by size:
2573 2011 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5740
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2605 3471 4429
Size: 416 53 83 399 24 1188 866 958
Cuts at: 4429 442
Size: 1753
Fragments arranged by size:
1753 1188 958 866 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2555 4400 1239
Size: 1316 1845 2579
Fragments arranged by size:
2579 1845 1316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3865 4730 5028 3865
Size: 865 298 4577
Fragments arranged by size:
4577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4403 882
Size: 617 2904 2219
Fragments arranged by size:
2904 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3531 493
Size: 711 2327 2702
Fragments arranged by size:
2702 2327 711
BsaBI GATnn'nnATC
Cuts at: 2171 5734 2171
Size: 3563 2177
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3547 3929 4564 275
Size: 53 83 186 936 2014 382 635 1451
Fragments arranged by size:
2014 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4173 4571 4845 4992 5403 4173
Size: 398 274 147 411 4510
Fragments arranged by size:
4510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2302 2332 1178
Size: 30 165 30 899 30 4586
Fragments arranged by size:
4586 899 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2323 1186
Size: 1137 4603
BseYI C'CCAG_C
Cuts at: 4943 5439 4943
Size: 496 5244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2057 2177 1163
Size: 573 321 120 4726
Fragments arranged by size:
4726 573 321 120
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3190 3951 4979 1540
Size: 275 186 1189 761 1028 2301
Fragments arranged by size:
2301 1189 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3801 3886 4957 3801
Size: 85 1071 4584
Fragments arranged by size:
4584 1071 85
BsmI GAATG_Cn'
Cuts at: 3251 3344 3251
Size: 93 5647
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3059 3801 3886 4957 1391
Size: 399 1269 742 85 1071 2174
Fragments arranged by size:
2174 1269 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 3629 5359 3629
Size: 1730 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2339 3095 844
Size: 1495 756 3489
Fragments arranged by size:
3489 1495 756
BsrBI CCG'CTC
Cuts at: 1394 1823 3627 1394
Size: 429 1804 3507
Fragments arranged by size:
3507 1804 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2397 4235 4409 65
Size: 1183 1149 1838 174 1396
Fragments arranged by size:
1838 1396 1183 1149 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4384 4571 1700
Size: 110 2574 187 2869
Fragments arranged by size:
2869 2574 187 110
BsrGI T'GTAC_A
Cuts at: 96 2109 2490 96
Size: 2013 381 3346
Fragments arranged by size:
3346 2013 381
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 2817 1184
Size: 1633 4107
BstYI r'GATC_y
Cuts at: 1972 2093 2475 3070 3437 3822 3839 5291 5377
Size: 121 382 595 367 385 17 1452 86
Cuts at: 5377 5389 5735 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 595 385 382 367 346 121
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2951 513
Size: 552 1886 3302
Fragments arranged by size:
3302 1886 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 3256 4050 4070 3256
Size: 794 20 4926
Fragments arranged by size:
4926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 2997 3044 2997
Size: 47 5693
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2417 4747 809
Size: 507 1101 2330 1802
Fragments arranged by size:
2330 1802 1101 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2751 3187 4076
Size: 54 1425 50 275 18 921 436 889
Cuts at: 4076 5705 8
Size: 1629 43
Fragments arranged by size:
1629 1425 921 889 436 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3187 1537
Size: 275 1375 4090
Fragments arranged by size:
4090 1375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2362 3670 1353
Size: 1009 1308 3423
Fragments arranged by size:
3423 1308 1009
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4327 4701 4847 387
Size: 1064 138 2738 374 146 1280
Fragments arranged by size:
2738 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2251 2555 3803 4400 5187 5455 1239
Size: 393 619 304 1248 597 787 268 1524
Fragments arranged by size:
1524 1248 787 619 597 393 304 268
EcoO109I rG'GnC_Cy
Cuts at: 2064 2064
Size: 5740
EcoRI G'AATT_C
Cuts at: 3049 3427 3049
Size: 378 5362
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 4887 1678
Size: 3209 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2528 2560 2600 2632 4381
Size: 32 135 32 956 32 40 32 1749
Cuts at: 4381 4413 4455 4487 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1749 956 135 42 40 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2604 3084 3330 669
Size: 988 281 666 480 246 3079
Fragments arranged by size:
3079 988 666 480 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2225 4854 5038 1305
Size: 920 2629 184 2007
Fragments arranged by size:
2629 2007 920 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2753 10
Size: 54 1425 343 921 2997
Fragments arranged by size:
2997 1425 921 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 3698 4057 4216 518
Size: 648 1299 1233 359 159 2042
Fragments arranged by size:
2042 1299 1233 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2191 2496 3156 3833 4981 5226 1606
Size: 435 150 305 660 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 660 435 305 245 150
NcoI C'CATG_G
Cuts at: 513 1065 2951 513
Size: 552 1886 3302
Fragments arranged by size:
3302 1886 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 3098 4643 1132
Size: 97 1869 1545 2229
Fragments arranged by size:
2229 1869 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2782 1348
Size: 1434 4306
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5740
PfoI T'CCnGG_A
Cuts at: 2471 2471
Size: 5740
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2064 2064
Size: 5740
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 2864 2910 3310 2864
Size: 46 400 5294
Fragments arranged by size:
5294 400 46
PstI C_TGCA'G
Cuts at: 830 3092 830
Size: 2262 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
PvuII CAG'CTG
Cuts at: 2041 2191 2041
Size: 150 5590
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 3088 4223 4904 5095 826
Size: 219 2043 1135 681 191 1471
Fragments arranged by size:
2043 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 2099 3067 1872
Size: 227 968 4545
Fragments arranged by size:
4545 968 227
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3844 4745 5007 820
Size: 197 926 1901 901 262 1553
Fragments arranged by size:
1901 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 3664 5
Size: 47 3612 2081
Fragments arranged by size:
3612 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2951 3179 513
Size: 552 1886 228 3074
Fragments arranged by size:
3074 1886 552 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2237 2587 3767 3784 3937 4122 4567
Size: 1255 657 350 1180 17 153 185 445
Cuts at: 4567 325
Size: 1498
Fragments arranged by size:
1498 1255 1180 657 445 350 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2109 2318 2490
Size: 274 80 33 51 1443 132 209 172
Cuts at: 2490 3986 5698 96
Size: 1496 1712 138
Fragments arranged by size:
1712 1496 1443 274 209 172 138 132
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 5700 5732 2085
Size: 32 3583 32 2093
Fragments arranged by size:
3583 2093 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 2097 3065 1870
Size: 227 968 4545
Fragments arranged by size:
4545 968 227
XmnI GAAnn'nnTTC
Cuts at: 3869 3869
Size: 5740
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI