Restriction map for
pFN21AE0836
(Circular) (Six-base) MAPSORT of: pFN21AE0836.seq Check: 2303 from: 1 to: 5659
pFN21AE0836 5659 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3002 1937
Size: 1065 4594
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2425 2881 2945 3722 5106 5374 1632
Size: 793 456 64 777 1384 268 1917
Fragments arranged by size:
1917 1384 793 777 456 268 64
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2663 1184
Size: 1479 4180
AlwNI CAG_nnn'CTG
Cuts at: 2904 3402 4974 2904
Size: 498 1572 3589
Fragments arranged by size:
3589 1572 498
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2576 2834 2968 3194 3346 5455 1493
Size: 1083 258 134 226 152 2109 1697
Fragments arranged by size:
2109 1697 1083 258 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2984 1870
Size: 73 1041 4545
Fragments arranged by size:
4545 1041 73
AvrII C'CTAG_G
Cuts at: 2188 2188
Size: 5659
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2980 4435 618
Size: 325 217 627 1193 1455 1842
Fragments arranged by size:
1842 1455 1193 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2457 2715 2978 1391
Size: 1066 258 263 4072
Fragments arranged by size:
4072 1066 263 258
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2344 2383 2674 928
Size: 971 445 39 291 3913
Fragments arranged by size:
3913 971 445 291 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5659
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4270 136
Size: 107 122 71 631 605 2598 1525
Fragments arranged by size:
2598 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 3720 4876 1790
Size: 1930 1156 2573
Fragments arranged by size:
2573 1930 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2709 3390 4348
Size: 416 53 83 399 24 1292 681 958
Cuts at: 4348 442
Size: 1753
Fragments arranged by size:
1753 1292 958 681 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2443 4319 1239
Size: 1204 1876 2579
Fragments arranged by size:
2579 1876 1204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2926 3784 4649 4947 2926
Size: 858 865 298 3638
Fragments arranged by size:
3638 865 858 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2007 4322 882
Size: 617 508 2315 2219
Fragments arranged by size:
2315 2219 617 508
BsaAI yAC'GTr
Cuts at: 493 1204 3450 493
Size: 711 2246 2702
Fragments arranged by size:
2702 2246 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3466 3848 4483 275
Size: 53 83 186 936 1933 382 635 1451
Fragments arranged by size:
1933 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2554 2584 1178
Size: 30 165 30 1151 30 4253
Fragments arranged by size:
4253 1151 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2741 1186
Size: 1555 4104
BseYI C'CCAG_C
Cuts at: 4862 5358 4862
Size: 496 5163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2741 2889 1163
Size: 573 1005 148 3933
Fragments arranged by size:
3933 1005 573 148
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3720 3805 4876 3720
Size: 85 1071 4503
Fragments arranged by size:
4503 1071 85
BsmI GAATG_Cn'
Cuts at: 2320 3170 3263 2320
Size: 850 93 4716
Fragments arranged by size:
4716 850 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2457 2715 2978 3720 3805 4876 1391
Size: 399 667 258 263 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 667 399 263 258 85
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2798 3014 844
Size: 1954 216 3489
Fragments arranged by size:
3489 1954 216
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2113 4154 4328 65
Size: 1183 865 2041 174 1396
Fragments arranged by size:
2041 1396 1183 865 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5659
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 2065 1184
Size: 881 4778
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2062
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2030 2989 3356 3741 3758 5210 5296 5308
Size: 58 959 367 385 17 1452 86 12
Cuts at: 5308 5654 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 959 385 367 346 86 58
17 12
Bsu36I CC'TnA_GG
Cuts at: 2698 2698
Size: 5659
BtgI C'CryG_G
Cuts at: 513 1065 2429 513
Size: 552 1364 3743
Fragments arranged by size:
3743 1364 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 2118 2619 3175 3969 3989 2118
Size: 501 556 794 20 3788
Fragments arranged by size:
3788 794 556 501 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2833 2963 2833
Size: 130 5529
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3106 3995 5624
Size: 54 1425 50 275 18 1276 889 1629
Cuts at: 5624 8
Size: 43
Fragments arranged by size:
1629 1425 1276 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2444 2591 2774 2900 2921 3589 1353
Size: 1091 147 183 126 21 668 3423
Fragments arranged by size:
3423 1091 668 183 147 126 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4246 4620 4766 387
Size: 1064 138 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2425 2443 2881 2945 3722 4319 5106
Size: 393 793 18 438 64 777 597 787
Cuts at: 5106 5374 1239
Size: 268 1524
Fragments arranged by size:
1524 793 787 777 597 438 393 268
64 18
EcoO109I rG'GnC_Cy
Cuts at: 2193 2193
Size: 5659
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 4806 1678
Size: 3128 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4300 4332 4374 4406 1373
Size: 32 135 32 2728 32 42 32 2626
Fragments arranged by size:
2728 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4773 4957 1305
Size: 3468 184 2007
Fragments arranged by size:
3468 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3837
Fragments arranged by size:
3837 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2639 3617 3976 4135 518
Size: 648 1473 978 359 159 2042
Fragments arranged by size:
2042 1473 978 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3075 3752 4900 5145 1606
Size: 1469 677 1148 245 2120
Fragments arranged by size:
2120 1469 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2429 513
Size: 552 1364 3743
Fragments arranged by size:
3743 1364 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 3017 4562 1132
Size: 97 1788 1545 2229
Fragments arranged by size:
2229 1788 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2328 1108
Size: 1220 4439
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2952 3728 3760 5241 5273 2920
Size: 32 776 32 1481 32 3306
Fragments arranged by size:
3306 1481 776 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 2214 2286 2809 2909 3011 830
Size: 1384 72 523 100 102 3478
Fragments arranged by size:
3478 1384 523 102 100 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SapI GCTCTTCn'nnn_
Cuts at: 2444 2774 2900 2444
Size: 330 126 5203
Fragments arranged by size:
5203 330 126
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2210 2282 2448 2805 2905 3007 4142
Size: 219 1165 72 166 357 100 102 1135
Cuts at: 4142 4823 5014 826
Size: 681 191 1471
Fragments arranged by size:
1471 1165 1135 681 357 219 191 166
102 100 72
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2941 3763 4664 4926 820
Size: 197 926 998 822 901 262 1553
Fragments arranged by size:
1553 998 926 901 822 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5659
StyI C'CwwG_G
Cuts at: 513 1065 2188 2429 2534 3098 513
Size: 552 1123 241 105 564 3074
Fragments arranged by size:
3074 1123 564 552 241 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2131 2396 2409 2830 3686 3703 3856
Size: 1255 551 265 13 421 856 17 153
Cuts at: 3856 4041 4486 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 856 551 445 421 265 185
153 17 13
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2278 3905 5617
Size: 274 80 33 51 1443 301 1627 1712
Cuts at: 5617 96
Size: 138
Fragments arranged by size:
1712 1627 1443 301 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 2554 2586 5619 5651 2022
Size: 32 500 32 3033 32 2030
Fragments arranged by size:
3033 2030 500 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI