Restriction map for
pFN21AE0822
(Circular) (Six-base) MAPSORT of: pFN21AE0822.seq Check: 9357 from: 1 to: 5401
pFN21AE0822 5401 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2551 3211 278
Size: 53 83 186 936 1015 660 2468
Fragments arranged by size:
2468 1015 936 660 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2744 1937
Size: 807 4594
Acc65I G'GTAC_C
Cuts at: 2722 2722
Size: 5401
AclI AA'CG_TT
Cuts at: 1563 3528 3901 1563
Size: 1965 373 3063
Fragments arranged by size:
3063 1965 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3464 4848 5116 1632
Size: 1832 1384 268 1917
Fragments arranged by size:
1917 1832 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2245 820
Size: 197 1228 3976
Fragments arranged by size:
3976 1228 197
AflIII A'CryG_T
Cuts at: 1203 2337 2418 2483 4300 5277 1203
Size: 1134 81 65 1817 977 1327
Fragments arranged by size:
1817 1327 1134 977 81 65
AgeI A'CCGG_T
Cuts at: 4232 4232
Size: 5401
AhdI GACnn_n'nnGTC
Cuts at: 4130 4130
Size: 5401
AleI CACnn'nnGTG
Cuts at: 1166 2603 1166
Size: 1437 3964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2116 1184
Size: 932 4469
AlwNI CAG_nnn'CTG
Cuts at: 3144 4716 3144
Size: 1572 3829
ApaLI G'TGCA_C
Cuts at: 3458 4614 3458
Size: 1156 4245
ApoI r'AATT_y
Cuts at: 1493 2014 2710 2936 3088 5197 1493
Size: 521 696 226 152 2109 1697
Fragments arranged by size:
2109 1697 696 521 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3955 160
Size: 872 2923 1606
Fragments arranged by size:
2923 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
AvaI C'yCGr_G
Cuts at: 1870 1943 2726 1870
Size: 73 783 4545
Fragments arranged by size:
4545 783 73
BamHI G'GATC_C
Cuts at: 2731 3098 2731
Size: 367 5034
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2722 4177 618
Size: 325 217 627 935 1455 1842
Fragments arranged by size:
1842 1455 935 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2720 1391
Size: 1329 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2039 928
Size: 971 140 4290
Fragments arranged by size:
4290 971 140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3592 3626 1903
Size: 34 50 34 1571 34 3678
Fragments arranged by size:
3678 1571 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3293 4503 3293
Size: 1210 4191
BclI T'GATC_A
Cuts at: 1548 2400 1548
Size: 852 4549
BfrBI ATG'CAT
Cuts at: 2539 2539
Size: 5401
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4012 136
Size: 107 122 71 631 605 2340 1525
Fragments arranged by size:
2340 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5396 5396
Size: 5401
BlpI GC'TnA_GC
Cuts at: 2818 2818
Size: 5401
Bme1580I G_kGCm'C
Cuts at: 1790 3462 4618 1790
Size: 1672 1156 2573
Fragments arranged by size:
2573 1672 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5401
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3132 4090 442
Size: 416 53 83 399 24 1715 958 1753
Fragments arranged by size:
1753 1715 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4061 1239
Size: 2822 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3526 4391 4689 3526
Size: 865 298 4238
Fragments arranged by size:
4238 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4064 882
Size: 617 2565 2219
Fragments arranged by size:
2565 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2340 3192 493
Size: 711 1136 852 2702
Fragments arranged by size:
2702 1136 852 711
BsaBI GATnn'nnATC
Cuts at: 5395 5395
Size: 5401
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2548 3208 3590 4225
Size: 53 83 186 936 1015 660 382 635
Cuts at: 4225 275
Size: 1451
Fragments arranged by size:
1451 1015 936 660 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3834 4232 4506 4653 5064 3834
Size: 398 274 147 411 4171
Fragments arranged by size:
4171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2246 2276 1178
Size: 30 165 30 843 30 4303
Fragments arranged by size:
4303 843 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2301 1186
Size: 1115 4286
BseYI C'CCAG_C
Cuts at: 4604 5100 4604
Size: 496 4905
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4828
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2851 3612 4640 1540
Size: 275 186 850 761 1028 2301
Fragments arranged by size:
2301 1028 850 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 3462 3547 4618 2100
Size: 1362 85 1071 2883
Fragments arranged by size:
2883 1362 1071 85
BsmI GAATG_Cn'
Cuts at: 2912 3005 2912
Size: 93 5308
BsmBI CGTCTCn'nnnn_
Cuts at: 2539 2539
Size: 5401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2720 3462 3547 4618 1391
Size: 399 310 620 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 620 399 310 85
BspEI T'CCGG_A
Cuts at: 5064 5064
Size: 5401
BspHI T'CATG_A
Cuts at: 3290 5020 3290
Size: 1730 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2039 2756 844
Size: 1195 717 3489
Fragments arranged by size:
3489 1195 717
BsrBI CCG'CTC
Cuts at: 1394 1823 3288 1394
Size: 429 1465 3507
Fragments arranged by size:
3507 1465 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2076 2150 3896 4070 65
Size: 1183 828 74 1746 174 1396
Fragments arranged by size:
1746 1396 1183 828 174 74
BsrFI r'CCGG_y
Cuts at: 1700 1810 2372 4045 4232 1700
Size: 110 562 1673 187 2869
Fragments arranged by size:
2869 1673 562 187 110
BsrGI T'GTAC_A
Cuts at: 96 2361 96
Size: 2265 3136
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5401
BssSI C'ACGA_G
Cuts at: 3461 4473 3461
Size: 1012 4389
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5401
BstBI TT'CG_AA
Cuts at: 4205 4205
Size: 5401
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5401
BstYI r'GATC_y
Cuts at: 1972 2731 3098 3483 3500 4952 5038 5050 5396
Size: 759 367 385 17 1452 86 12 346
Cuts at: 5396 1972
Size: 1977
Fragments arranged by size:
1977 1452 759 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2639 2639
Size: 5401
BtgI C'CryG_G
Cuts at: 513 1065 2565 513
Size: 552 1500 3349
Fragments arranged by size:
3349 1500 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4662
Fragments arranged by size:
4662 683 56
BtsI GCAGTG_nn'
Cuts at: 2069 2098 2917 3711 3731 2069
Size: 29 819 794 20 3739
Fragments arranged by size:
3739 819 794 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5366
DraI TTT'AAA
Cuts at: 2705 2705
Size: 5401
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4408 809
Size: 507 3092 1802
Fragments arranged by size:
3092 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2848 3737
Size: 54 1425 50 275 18 306 712 889
Cuts at: 3737 5366 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 712 306 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2848 1537
Size: 275 1036 4090
Fragments arranged by size:
4090 1036 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2400 2534 3331 1353
Size: 1047 134 797 3423
Fragments arranged by size:
3423 1047 797 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3988 4362 4508 387
Size: 1064 138 2399 374 146 1280
Fragments arranged by size:
2399 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3464 4061 4848 5116 1239
Size: 393 1832 597 787 268 1524
Fragments arranged by size:
1832 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2250 2250
Size: 5401
EcoRI G'AATT_C
Cuts at: 2710 3088 2710
Size: 378 5023
FspI TGC'GCA
Cuts at: 3907 3907
Size: 5401
HaeII r_GCGC'y
Cuts at: 1678 4548 1678
Size: 2870 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4042 4074 4116 4148 1373
Size: 32 135 32 2470 32 42 32 2626
Fragments arranged by size:
2626 2470 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2745 2991 669
Size: 988 281 807 246 3079
Fragments arranged by size:
3079 988 807 281 246
HindIII A'AGCT_T
Cuts at: 748 2472 748
Size: 1724 3677
HpaI GTT'AAC
Cuts at: 2991 2991
Size: 5401
KpnI G_GTAC'C
Cuts at: 2726 2726
Size: 5401
MfeI C'AATT_G
Cuts at: 3000 3000
Size: 5401
MluI A'CGCG_T
Cuts at: 5277 5277
Size: 5401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4515 4699 1305
Size: 3210 184 2007
Fragments arranged by size:
3210 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2138 10
Size: 54 1425 343 306 3273
Fragments arranged by size:
3273 1425 343 306 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2081 2603 3359 3718 3877 518
Size: 648 915 522 756 359 159 2042
Fragments arranged by size:
2042 915 756 648 522 359 159
MspA1I CmG'CkG
Cuts at: 1606 2817 3494 4642 4887 1606
Size: 1211 677 1148 245 2120
Fragments arranged by size:
2120 1211 1148 677 245
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 5401
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4849
NdeI CA'TA_TG
Cuts at: 387 2122 387
Size: 1735 3666
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 5401
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5401
NotI GC'GGCC_GC
Cuts at: 2848 2848
Size: 5401
NsiI A_TGCA'T
Cuts at: 2541 2541
Size: 5401
NspI r_CATG'y
Cuts at: 1132 1229 2107 2331 2422 2487 2539 2759 4304
Size: 97 878 224 91 65 52 220 1545
Cuts at: 4304 1132
Size: 2229
Fragments arranged by size:
2229 1545 878 224 220 97 91 65
52
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5401
PciI A'CATG_T
Cuts at: 2418 2483 4300 2418
Size: 65 1817 3519
Fragments arranged by size:
3519 1817 65
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5401
PmeI GTTT'AAAC
Cuts at: 2705 2705
Size: 5401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 4983 5015 3470
Size: 32 1481 32 3856
Fragments arranged by size:
3856 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2250 2250
Size: 5401
PshAI GACnn'nnGTC
Cuts at: 1613 2504 1613
Size: 891 4510
PsiI TTA'TAA
Cuts at: 2971 2971
Size: 5401
PstI C_TGCA'G
Cuts at: 830 2458 2753 830
Size: 1628 295 3478
Fragments arranged by size:
3478 1628 295
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5401
SalI G'TCGA_C
Cuts at: 1936 2743 1936
Size: 807 4594
SbfI CC_TGCA'GG
Cuts at: 2753 2753
Size: 5401
ScaI AGT'ACT
Cuts at: 3649 5361 3649
Size: 1712 3689
SexAI A'CCwGG_T
Cuts at: 2193 2193
Size: 5401
SfcI C'TryA_G
Cuts at: 826 1045 2158 2377 2454 2749 3884 4565 4756
Size: 219 1113 219 77 295 1135 681 191
Cuts at: 4756 826
Size: 1471
Fragments arranged by size:
1471 1135 1113 681 295 219 219 191
77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5401
SmaI CCC'GGG
Cuts at: 1872 2728 1872
Size: 856 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2245 3505 4406 4668 820
Size: 197 926 302 1260 901 262 1553
Fragments arranged by size:
1553 1260 926 901 302 262 197
SnaBI TAC'GTA
Cuts at: 493 3192 493
Size: 2699 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5401
SphI G_CATG'C
Cuts at: 1132 2331 2539 2759 1132
Size: 1199 208 220 3774
Fragments arranged by size:
3774 1199 220 208
SspI AAT'ATT
Cuts at: 5 52 3325 5
Size: 47 3273 2081
Fragments arranged by size:
3273 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5401
StyI C'CwwG_G
Cuts at: 513 1065 2176 2840 513
Size: 552 1111 664 3074
Fragments arranged by size:
3074 1111 664 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3428 3445 3598 3783 4228 325
Size: 1255 1848 17 153 185 445 1498
Fragments arranged by size:
1848 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2361 3647 5359
Size: 274 80 33 51 1443 384 1286 1712
Cuts at: 5359 96
Size: 138
Fragments arranged by size:
1712 1443 1286 384 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5361 5393 5361
Size: 32 5369
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5401
XbaI T'CTAG_A
Cuts at: 2737 2737
Size: 5401
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5401
XmaI C'CCGG_G
Cuts at: 1870 2726 1870
Size: 856 4545
XmnI GAAnn'nnTTC
Cuts at: 2449 3530 2449
Size: 1081 4320
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2549 3209 276
Size: 53 83 186 936 1015 660 2468
Fragments arranged by size:
2468 1015 936 660 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI